Labshake search
Citations for Lonza :
1151 - 1200 of 1258 citations for Fipronil 3 Cyano Pyrazole 3 4 5 13C4 99%; 3 Cyano 5 15N2 98% 100 Ug Ml In Methanol since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Evolutionary Biology 2019Quote: ... inactivated trypsin by adding 2 volumes of medium (500 ml DMEM (Lonza, Catalog no. BE12-733F), 55 ml FBS (PAN ...
-
bioRxiv - Microbiology 2021Quote: ... Agarose-medium was made by autoclaving a 17.6 µg/ml SeaKem ME agarose (Lonza, Basel, Switzerland) dilution and heating it to 65°C ...
-
bioRxiv - Immunology 2022Quote: ... Absence of endotoxins (< 1EU/mL) was confirmed using the PyroGene™ Recombinant Factor C Assay (Lonza) according to manufacturer’s instructions.
-
bioRxiv - Neuroscience 2023Quote: ... and the cell pellet resuspended in 2 ml of ACK lysing buffer (Lonza, Cat# 10-548E). After centrifugation ...
-
bioRxiv - Cancer Biology 2022Quote: Primary OCSC cultures were seeded at 5000 cells/ml in MEBM™ (Lonza, cat# CC-3151) supplemented with 2 mM L-glutamine ...
-
bioRxiv - Bioengineering 2021Quote: ... We then electroporated 4.0×105 of wildtype or the stably integrated mCherry K562 cell lines in 100 μL Amaxa solution (Lonza Nucleofector 2b, program T-016) with pJT396 in combination with an equimolar amount of either pUC19 or a Cp36 expression vector ...
-
bioRxiv - Cancer Biology 2021Quote: ... cells were also transfected with electroporation using Amaxa™ Nucleofector Kit R (Cat.no.VCA-100) and the Nucleofector™II device (program W-001, Lonza Group AG, Cologne, Germany) as suggested by the manufacturer.
-
bioRxiv - Neuroscience 2019Quote: ... the dissociated neurons were centrifuged down to remove the supernatant and resuspended in 80 to 100 μl of Amaxa electroporation buffer for mouse neurons (Lonza Cologne GmbH; Cologne, Germany) with mixtures of siRNA (0.2 nmol per transfection ...
-
bioRxiv - Biochemistry 2023Quote: ... Mm.Cas9.NOS2.1.AA) and 1 µM HiFi Cas9 enzyme (IDT, catalog no. 1081060) in 100 µL Nucleofector Solution V plus supplement (Lonza, catalog no. VCA-1003), using the preprogrammed electroporation protocol B-032 on Nucleofector II/2b ...
-
bioRxiv - Neuroscience 2023Quote: ... 4−106 cells were pelleted at 300xg and then resuspended in 100 μL prewarmed nucleofector solution (Human Stem Cell Kit 1, Lonza, catalog no. VPH-5012); 4 μg of NGN2 plasmid and 1.5 μg each of 1R and 1L plasmids were added directly to the resuspended cells ...
-
bioRxiv - Bioengineering 2021Quote: ... 1.0×106 K562 cells harboring a monoclonal Kp03 landing pad and multiclonal rTetR-VP48 expression construct were electroporated in 100 μL Amaxa solution (Lonza Nucleofector 2b, program T-016) with 1000 ng of reporter donor plasmid (pMMH56,59,59) ...
-
bioRxiv - Molecular Biology 2019Quote: ... 150,000 cells were resuspended in 20 µL of P3 Primary Cell Nucleofector Solution with Supplement 1 and mixed with the gRNA/rCas9 solutions before electroporation (4D-Nucleofector System, Lonza, Walkersville, MD; program DC-100). For experiments involving the Neon Transfection System (Thermo Fisher Scientific) ...
-
bioRxiv - Genomics 2021Quote: ... PBMCs were then treated with 2 ml of erythrocytes lysis buffer (Lonza Walkersville inc. #120-02-070) for 1 min ...
-
bioRxiv - Bioengineering 2021Quote: ... EV pellets obtained from approximately 90 mL CM were re-suspended in 150-200 µL PBS (Lonza). Protein concentration was determined with the Bradford assay.
-
bioRxiv - Molecular Biology 2020Quote: ... Days 16-24 media contained recombinant Oncostatin M (20ng/ml, Thermo) in HCM media lacking EGF (Lonza).
-
bioRxiv - Bioengineering 2023Quote: ... the ground bones in 50 ml tubes were washed with phosphate-buffered saline (PBS, Lonza, Slough, UK) containing 2% penicillin/streptomycin (P/S ...
-
bioRxiv - Microbiology 2023Quote: ... siRNAs (1 μg per ml) were nucleofected into ISE6 cells using the 4D-Nucleofector System (Lonza Bioscience). Tick cells were centrifuged at 100 x g for 10 minutes to pellet the cells ...
-
bioRxiv - Cell Biology 2019Quote: ... Minced tissue were put into a 15-mL falcon tube filled with 3mL EBM-2MV (Lonza, Cologne, Germany) supplemented with 0.5% FBS ...
-
bioRxiv - Bioengineering 2019Quote: ... cell culture surfaces were treated with 20 μg/mL PDL diluted in sterile cell culture sterile water (Lonza) overnight ...
-
bioRxiv - Cancer Biology 2020Quote: ... and 10 ng/mL basic fibroblast growth factor or MEGM™ culture medium (Lonza, cat. no. CC-3150), completed as per manufacturer’s protocol ...
-
bioRxiv - Cell Biology 2021Quote: ... at 0.1-1 μg/mL or as a control a GFP plasmid (PmaxGFP, Lonza, Cat. No. D-00059) using Lipofectamine 3000 (Invitrogen ...
-
bioRxiv - Immunology 2019Quote: ... 0.8×106 CD8+ enriched PBMCs/well were seeded with 1µg/mL soluble αCD28 (clone CD28.2, eBioscience) in 1mL IMDM (LONZA) supplemented with 8% Fetal Calf Serum ...
-
bioRxiv - Bioengineering 2019Quote: ... or γδ-T cells were activated and cultured for 2 days at 0.5 to 1.0 million cells/mL in XVivo15 medium (Lonza) with 5% Fetal Bovine Serum ...
-
bioRxiv - Molecular Biology 2021Quote: ... at a density of 2 million cells/mL in serum-free cell culture medium (Lonza AG, Basel, Switzerland) supplemented with 0.5 μg/mL FMS-like tyrosine kinase-3 (Peprotech ...
-
bioRxiv - Microbiology 2022Quote: ... diluted in an equal volume of DMEM containing 20% FBS and 200 U/ml of penicillin/streptomycin (Lonza), and ∼1.5×104 hemolymph sporozoites were added per well ...
-
bioRxiv - Microbiology 2022Quote: ... Agarose-medium was made by autoclaving a dilution of 17.6 μg/ml SeaKem ME agarose (Lonza, Basel, Switzerland) and heating it to 65°C ...
-
bioRxiv - Pathology 2023Quote: ... and the cell pellet was resuspended in 10 mL of Endothelial Cell Growth Medium-2 (EGM-2, Lonza). The cell suspension was plated into a 75 cm2 tissue culture treated flask and cultured in a 5% CO2 incubator at 37°C ...
-
bioRxiv - Molecular Biology 2023Quote: ... 6-well dishes were seeded with 1.0 × 106 Sf9 cells per well in 2.0 mL InsectExpress medium (Lonza). Cells were transfected with 20 mL of bacmid per well ...
-
bioRxiv - Microbiology 2021Quote: ... 500 EBs were transferred per T175 tissue culture flasks in 20 mL X-VIVO™15 (04-418Q, Lonza), supplemented with 100 ng mL-1 M-CSF (PHC9504 ...
-
bioRxiv - Neuroscience 2021Quote: ... High Five cells were seeded 1 day prior to infection at 1×106 cells/mL in InsectXpress medium (Lonza). Cells were co-infected with low-passage recombinant baculovirus for COL6A3WT or COL6A3ΔC4 along with P4HA1/P4HB at a MOI of 2 and 0.2 respectively ...
-
bioRxiv - Immunology 2020Quote: ... Colons were incubated 2 times at 200 rpm in 40 mL HBSS + 0.1% BSA + 1% Penicillin-Streptomycin (PS, Lonza) + 5mM EDTA (Sigma-Aldrich ...
-
bioRxiv - Bioengineering 2021Quote: ... CD3+ T cells were debeaded with an EasySep magnet (STEMCELL) prior to resuspension and electroporation at 0.5-1×106 cell mL−1 in P3 buffer (Lonza). Following electroporations with RNPs and HDRTs ...
-
bioRxiv - Biochemistry 2022Quote: ... the media supernatant was removed and we washed the cells with 2 ml of prewarmed 1xDPBS (Lonza, 17-512F). The PBS was then removed and cells were then incubated at 37°C in 250 μL of trypsin (Corning ...
-
bioRxiv - Microbiology 2019Quote: ... the monolayers were washed three times with ice-cold PBS and overlaid with 2 ml of Joklik’s minimal essential medium (Lonza) supplemented with 5% fetal bovine serum (Life Technologies) ...
-
bioRxiv - Microbiology 2019Quote: ... the monolayers were washed three times with ice-cold PBS and overlaid with 2 ml of Joklik’s minimal essential medium (Lonza) supplemented with 5% fetal bovine serum (Life Technologies) ...
-
bioRxiv - Microbiology 2019Quote: ... the monolayers were washed three times with ice-cold PBS and overlaid with 2 ml of Joklik’s minimal essential medium (Lonza) supplemented with 5% fetal bovine serum (Life Technologies) ...
-
bioRxiv - Microbiology 2019Quote: ... the monolayers were washed three times with ice-cold PBS and overlaid with 2 ml of Joklik’s minimal essential medium (Lonza) supplemented with 5% fetal bovine serum (Life Technologies) ...
-
bioRxiv - Cancer Biology 2020Quote: 1×105 cells were suspended in 1 ml of 0.33% select agar in small airway growth medium without EGF (Lonza) and plated on a bottom layer of 0.5% select agar in the same media in six-well dishes ...
-
bioRxiv - Immunology 2019Quote: ... Colons were incubated 2 times at 200 rpm in 40 mL HBSS + 0.1% BSA + 1% Penicillin-Streptomycin (PS, Lonza) + 5mM EDTA (Sigma-Aldrich ...
-
bioRxiv - Biochemistry 2021Quote: ... then the ventricles were dissected and dissociated by serial digestion at 37°C with 0.44 mg/ml (6800 U) Worthington Type II collagenase (supplied by Lonza) and 0.6 mg/ml pancreatin (Sigma-Aldrich ...
-
bioRxiv - Developmental Biology 2020Quote: ... Wells were mounted by pouring 1 ml of 2.5% melted agarose (50080, Lonza Rockland, ME in Danieau buffer (DBx1) into the μ-dish with the mold inside ...
-
bioRxiv - Cell Biology 2021Quote: ... The harvested EV pellet was resuspended in 1 mL RNase-free Phosphate buffered saline (RNase-free PBS, Lonza, Australia) and washed using ultracentrifugation at 100,000 × g for 60 min at 4°C ...
-
bioRxiv - Immunology 2021Quote: ... cells were washed with PBS and 1 ml of fresh Hepatocyte Culture Medium (HCM) was added per well (Lonza). At 6 or 72 hpi ...
-
bioRxiv - Immunology 2021Quote: ... washed in PBS by centrifugation for 5 min at 1300 rpm at 4°C and resuspended at 4.0 × 106 cells/ml in complete medium (IMDM 2 mM glutamax I supplemented with 8% heat-inactivated FCS (Lonza), 2% heat-inactivated chicken serum ...
-
bioRxiv - Immunology 2021Quote: ... Cells were centrifuged for 5 min at 1200 rpm at 20°C and resuspended at 2.5×106 cells/ml in X-VIVO 15 cell culture medium (Lonza) with 50 U/ml penicillin–streptomycin ...
-
bioRxiv - Immunology 2021Quote: ... 50 μg/mL Streptomycin and 50 μM β-mercaptoethanol) and regularly tested for mycoplasma (MycoAlert Mycoplasma Detection kit, Lonza). The B16-F1-mCherry and B16-F1-Azurite cell lines were generated by lentiviral transduction ...
-
bioRxiv - Developmental Biology 2023Quote: ... Smiths Medical) were connected to two 1-mL gas-tight syringes (SGE or Hamilton) and filled with PBS (Lonza). The tubing was manually filled with PBS ...
-
bioRxiv - Synthetic Biology 2024Quote: ... Isolated T cells were cultured at an initial density of 106 cells/mL in X-Vivo 15 medium (Lonza) supplemented with human serum (5% ...
-
bioRxiv - Neuroscience 2023Quote: ... containing ∼1000 neurons) was transferred to 9 mL of wash media (DMEM/F12 with penicillin/streptomycin [Lonza; Allendale, NJ]), then recovered by centrifugation (∼350 xG ...
-
bioRxiv - Molecular Biology 2024Quote: ... once the cell confluency reached 90% the culture medium was changed to 10 mL XVIVO-10 medium (Lonza #(BE)BP04-743Q ...