Labshake search
Citations for Lonza :
1151 - 1200 of 1401 citations for 6 AMINO 3 HYDROXY PYRIDO 2 3 B PYRAZINE 2 CARBOXYLIC ACID since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Microbiology 2021Quote: ... Cells were infected for 30 min with exponential-phase bacteria at a multiplicity of infection (MOI) of 2 and treated with 20 μg/ml of gentamicin (Lonza) for 60 min as described [22] ...
-
bioRxiv - Microbiology 2021Quote: Purified SARS-CoV-2 RBD and SED (Spike ectodomain) were diluted in DPBS (without Ca and Mg, Lonza, 17-512F) and coated onto 96-well plates (MaxiSorp - ThermoFisher or High binding - Greiner Bio-one ...
-
bioRxiv - Immunology 2021Quote: ... The inoculum was then removed and replaced with 2 ml of DMEM complete medium with 1% SeaPlaque agarose (Cat# 50100, Lonza). Plaques were visualized using Neutral red (Sigma-Aldrich ...
-
bioRxiv - Cell Biology 2019Quote: ... 2 x 106 cells of MEFs were transfected with 1.2 nM of siRNAs using Amaxa Nucleofector II (Lonza, Basel, Switzerland) and MF 1 Nucleofector kit (Lonza ...
-
bioRxiv - Molecular Biology 2019Quote: ... Primary HBECs (human bronchial epithelial cells) were grown in 2% oxygen using a 1:1 mixture of bronchial epithelial basal medium (BEBM; Lonza) and DMEM and supplemented with SingleQuot (Lonza ...
-
bioRxiv - Microbiology 2021Quote: ... HeLa (ATCC® CCL-2™), and HaCaT cells (gift from Jonathan Garlick, Tufts University) were grown in Dulbecco’s modified Eagle medium (DMEM; Lonza) containing high glucose ...
-
bioRxiv - Cancer Biology 2020Quote: ... murine 3B11 and human HUVECs endothelial cells (all purchased from ATCC) were cultured in RPMI 1640+ 10% Fetal Bovine Serum (FBS) or in endothelial grown media (EGM-2-Lonza).
-
bioRxiv - Immunology 2021Quote: ... The inoculum was then removed and replaced with 2□ml of DMEM complete medium with 1% SeaPlaque agarose (Cat. # 50100, Lonza). The cells were incubated at 37 °C ...
-
bioRxiv - Cell Biology 2019Quote: ... EGM-2 was prepared by combining components of an EGM-2 “bullet kit” (Cat no. CC-4176) and endothelial basal medium (EBM, Lonza, Walkersville ...
-
bioRxiv - Immunology 2021Quote: ... and soluble anti-CD28 (500 ng/mL) at a density of 2 × 106 cells/mL of X-vivo 15 serum-free medium (Lonza). For iTreg differentiation ...
-
bioRxiv - Immunology 2020Quote: ... or media were cultured in 96 well flat bottom plates overnight then PBMC (2×105) from healthy donors were cultured at 37 °C in X-VIVO 15 media (Lonza) with 50-200 U/mL IL-2 (Peprotech) ...
-
bioRxiv - Immunology 2021Quote: Human telomerase-immortalized corneal epithelial (hTCEpi) cells (26) were maintained at 37°C/5% CO2 in regular keratinocyte growth medium KGM-2 (Lonza). Prior to treatment ...
-
Incidence of an intracellular multiplication niche amongst Acinetobacter baumannii clinical isolatesbioRxiv - Microbiology 2021Quote: ... pH 7.4 and grown in supplemented keratinocyte growth medium containing 0.15 mM CaCl2 (KBM-2 BulletKit, Lonza Biosciences, Basel, Switzerland) as previously described (38) ...
-
bioRxiv - Immunology 2021Quote: ... CD3/CD28 beads were removed 48 hours after stimulation and 5 × 106 CTLs were electroporated with 2 μg plasmid using 4D-Nucleofector (Lonza). Medium was changed 6 hours after nucleofection and transfected cells were used 24-36 hours after electroporation ...
-
bioRxiv - Neuroscience 2021Quote: ... the suspension was centrifuged (215g for 5min at 4°C) and the pellet was resuspended in BMEC-media that comprised of EBM-2 medium (Lonza) supplemented with the following ...
-
bioRxiv - Bioengineering 2022Quote: ... Bone marrow mononuclear cells were plated in endothelial growth medium 2 + 10% fetal bovine serum (FBS) (EGM-2MV, Lonza, Switzerland) on fibronectin coated plates and cultured at 37°C in 5% CO2 incubators ...
-
bioRxiv - Cell Biology 2022Quote: ... Three slices were placed in one well of a 12-well plate and incubated with 2 mL DMEM (Lonza, #42430) with 0.6% amphotericin B ...
-
bioRxiv - Systems Biology 2022Quote: ... The red blood cell lysis was performed by resuspending the pellet in 2 ml sterile ACK (Ammonium-Chloride-Potassium) lysis buffer (ACK Lysing Buffer, Lonza) and incubating on ice for 5 minutes ...
-
bioRxiv - Physiology 2022Quote: HUVEC were grown at 80% confluence (5×103 cells per well) on glass coverslips in a 24-well plate and starved overnight in serum-free medium (EBM-2, Lonza). They were then incubated for 24 hr in EGM2 containing 10μM BrdU ...
-
bioRxiv - Molecular Biology 2022Quote: ... HEK293 cells were washed twice with PBS and 2 × 106 cells were resuspended in 100 μl of Solution V (Lonza) and then mixed with 5 μg purified sgRNA expression vector or the empty vector as a negative control before nucleofection using program Q-001 of a Nucleofector™ 2b (Lonza) ...
-
bioRxiv - Molecular Biology 2024Quote: HUVEC were grown at 80% confluence (5×103 cells per well) on glass coverslips in a 24-well plate and starved overnight in serum-free medium (EBM-2, Lonza). They were then incubated for 24 hr in EGM2 containing 10µM BrdU ...
-
bioRxiv - Plant Biology 2024Quote: ... The fixed cells were washed three times with the same buffer and then embedded in 2 % low-melting-temperature agarose (SeaPlaque, Lonza). The embedded samples were post-fixed with 4 % potassium permanganate at 4 °C for 2 h ...
-
bioRxiv - Cell Biology 2024Quote: ... Large scale D.Mel-2 cultures were prepared by diluting cells to a density of 106 cells/mL in Insect Xpress culture medium (Lonza) in Erlenmeyer flasks ...
-
bioRxiv - Cancer Biology 2024Quote: ... by flushing one femur with PBS 2% FBS followed by erythrocyte elimination with ACK Lysing Buffer (10-548E, BioWhittaker, Lonza) (Figure 4BC) ...
-
bioRxiv - Bioengineering 2024Quote: ... HSPCs (2×105 cells/condition) were transfected with RNP complexes using the P3 Primary Cell 4D-Nucleofector X Kit (Lonza) and the CA137 program (Nucleofector 4D ...
-
bioRxiv - Cancer Biology 2024Quote: ... were isolated from 8 to 16-week-old Rptorfl/fl or Tsc2fl/fl mice and maintained in EGM-2 medium (Lonza), as previously described71–75 ...
-
bioRxiv - Cancer Biology 2024Quote: ... The OCI-LY3 cell line was cultured in IMDM with 25 mM HEPES and 2 mM L-glutamine (Lonza; Switzerland), supplemented with P/S ...
-
bioRxiv - Developmental Biology 2023Quote: ... 2 µg of PX459 V2.0 containing the sgRNA sequence were electroporated into 2×106 DVI2 ESCs (HM1 background) using program A023 of the Amaxa Nucleofector II (Lonza) to ablate endogenous Shh gene expression ...
-
bioRxiv - Cancer Biology 2023Quote: ... Cells were co-transfected with 2 µg of the CRISPR/Cas9 plasmid and 2 µg of ssODN (50 µM) using a Nucleofector II and Amaxa Nucleofector kits (Lonza) according to the manufacturer’s instructions ...
-
bioRxiv - Immunology 2023Quote: ... Transfections were performed with an Amaxa Nucleofector device: 2×106 cells were resuspended in 100 μL Nucleofector solution T (Lonza) and added to 2 µg of pcDNA3.1 expression vector encoding full-length IL-2Rβ ...
-
bioRxiv - Immunology 2023Quote: ... at bead-to-cell ratio of 1:1 in human IL-2 medium (X-VIVO 15, serum-free hematopoietic cell medium, with 2mM L-Glutamine and gentamicin (Lonza) supplemented with 5% Human AB serum (Valley Medical) ...
-
bioRxiv - Developmental Biology 2022Quote: ... Primary MEC were cultured in endothelial growth media (EGM) containing 5% FBS with growth factors (EBM-2; Lonza, Basel, Switzerland) at 37°C in 5% CO2 ...
-
bioRxiv - Genomics 2022Quote: ... cells (from two donors) were grown to 80% to 90% confluence in endothelial basal medium 2-MV with supplements (EBM; Lonza) and 5% fetal bovine serum (FBS ...
-
bioRxiv - Genomics 2023Quote: ... of the PCR products were analyzed by electrophoresis on a 2% Agarose S gel (Nippon gene) in 1× TBE buffer and stained with GelStar (Lonza). The remaining PCR product of the condition that displayed a single-band or near single-band product on the gel was double size-selected and purified using AMPure XP beads (Beckman coulter ...
-
bioRxiv - Cell Biology 2023Quote: ... Five million BJAB or OCI-LY1 cells were transfected with 2 μg plasmid DNA using the SF Cell Line 4D-Nucleofector X Kit L (Lonza) and the AMAXA Nucleofector biosystem (BJAB ...
-
bioRxiv - Molecular Biology 2023Quote: ... 1×105 cells were grown in each well of 12 well culture plates in SkGM-2 BulletKit growth medium (Lonza) to >90% confluency under standard culture conditions of 37°C and 5% CO2 ...
-
bioRxiv - Immunology 2023Quote: ... Mixtures of complexed sgRNA and Cas9 (0.3 nmol synthetic sgRNA + 62 µmol Cas9 nuclease) and 2-10×106 enriched murine CD8+ T cells were suspended in 25ul of P3 electroporation buffer (Lonza) and electroporated using the Lonza 4D Nucleofector (pulse code DN100) ...
-
bioRxiv - Molecular Biology 2023Quote: ... MRTF-WT and MRTF knockout in NIH 3T3 (MRTF-KO-1 and MRTF-KO-2) cells were cultured in DMEM (BE12-614Q, Lonza) supplemented with 10 % Fetal Bovine Serum (FBS ...
-
bioRxiv - Molecular Biology 2023Quote: ... A cell count ranging from 1 × 10^5 to 2 × 10^5 cells was collected and suspended in 15 µL of nucleofection buffer (Lonza). Electroporations were conducted in the strip format ...
-
bioRxiv - Immunology 2023Quote: ... and cultured in 96-well culture plates (2×103 cells/well) with serum or EP-stimulated MDMs supernatants in X-VIVO 15 medium (Lonza) with 10 U/mL of IL-2 (PeproTech ...
-
bioRxiv - Developmental Biology 2023Quote: ... An mTmG lens carrying the MLR39-Cre allele was placed in a small hole in the center of an agarose pad that was prepared by loading 1 mL of 2% agarose (SeaKem GTG, Lonza) in M199 on the surface of 35 mm glass-bottom dish (3970-035 ...
-
bioRxiv - Cell Biology 2023Quote: ... JAWS transfection was achieved using 20 μg DNA and 2 x 106 million cells per 100 μl nucleofection solution from the Amaxa Nucleofector Mouse Dendritic Cell kit (Lonza), and the Y-001 program ...
-
bioRxiv - Molecular Biology 2023Quote: ... spanning the whole SARS-CoV-2 genome was transfected into 2×106 HEK293T cells using the SF Cell Line 4D-Nucleofector-X Kit (Cat# V4XC-2012, Lonza) and the 4D-Nucleofector X Unit (Lonza ...
-
bioRxiv - Microbiology 2023Quote: ... Stable cell lines were generated by nucleofecting 2 µg of pBud-PCID2-Flag plasmid or empty pBud as control using Amaxa Nucleofector (Lonza) and Nucleofector Kit R (Lonza ...
-
bioRxiv - Cell Biology 2024Quote: ... 100 µL aliquots (7 × 106 cells) were then transferred into 2 mm EP cuvettes and electroporated on an Amaxa Nucleofector I (Lonza). For A2780 cells ...
-
bioRxiv - Immunology 2024Quote: ... 1-2*106 T-ALL cells were resuspended in 100uL P3 nucleofector solution (82 uL nucleofection solution + 18 uL supplement; Lonza P3 Primary Cell 4D-NucleofectorTM X Kit) ...
-
bioRxiv - Developmental Biology 2024Quote: ... we proceeded with 50% Basal media (+10uh/ml HGF) and 50% SkGM™-2 Skeletal Muscle Cell Growth Medium (Lonza). Subsequently ...
-
bioRxiv - Developmental Biology 2024Quote: ... A total of 2 × 104 cells were seeded in each well with 150 μl EC specific media (Lonza, CC-3202). TIFF images of capillary-like networks were captured using a Zeiss Axio Image M2 microscope equipped with a digital camera at 4 ...
-
bioRxiv - Immunology 2019Quote: ... HAE cultures were grown in B-ALI medium supplemented with inducer (Lonza Inc.) at each media change with provision of an air-liquid interface for approximately 6 weeks to form differentiated ...
-
bioRxiv - Developmental Biology 2021Quote: ... Nucleofection was performed with program B-016 on an Amaxa Nucleofector II (Lonza). Cells were then grown for ten days under selection by 1 μg/mL puromycin and 10 μg/mL blasticidin ...