Labshake search
Citations for Lonza :
1101 - 1150 of 1165 citations for High mobility group protein B1 HMGB1 Human HEK293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Cell Biology 2023Quote: ... MCF10A cells (female human mammary epithelial cells) were purchased from ATCC (CRL-10317) and cultured as recommended by ATCC in MEGM (Lonza CC-3150) supplemented with bovine pituitary extract ...
-
bioRxiv - Immunology 2023Quote: ... 10% FBS heat-inactivated and 1% Penicillin/Streptomycin. Human Small Airway Epithelial Cells (SAECs, Cat. CC-2547) and the corresponding medium were commercially obtained by Lonza (NJ, USA). Human Pulmonary Microvascular Endothelial Cells (HPMECs ...
-
bioRxiv - Molecular Biology 2023Quote: Induced pluripotent stem cells were transfected with a total of 5 μg target Cas9/gRNA plasmids with or without 1 μg repair templates using Human Stem Cell Nucleofector Kit (LONZA, Basel, Switzerland) and Nucleofector 2b device (LONZA ...
-
bioRxiv - Cell Biology 2023Quote: ... The linearized construct and the purified schizonts were mixed with Nucleofector solution from an Amaxa human T cell Nucleofector Kit and electroporated using the Amaxa Nucleofector II device (Lonza, Köln, Germany). Directly after transfection 50 µl RPMI-1640 complete was added to the transfection reaction followed by intravenous injection into one SWISS mouse ...
-
bioRxiv - Microbiology 2023Quote: ... BEAS-2B cells (human bronchial epithelial; CRL-9609ATCC) cells were grown in bronchial epithelial cell growth basal media (BEBM; Lonza/CC-3170). LLC-MK-2 cells (rhesus monkey kidney ...
-
bioRxiv - Genomics 2024Quote: ... we generated Unrelated Control 2 iPSC using purchased human dermal fibroblasts from a healthy unrelated male (CC-2511, Lot No. 0000293971, Lonza, Basel, Switzerland). We conducted fibroblast collection and culturing ...
-
bioRxiv - Cell Biology 2024Quote: ... 8 x 105 H9 hESCs in single-cell suspension were resuspended in 100 µL electroporation buffer from the Human Stem Cell Nucleofector Solution 2 kit (Lonza, VPH-5022). Alt-R Cas9 Electroporation enhancer (1.1 µM ...
-
bioRxiv - Neuroscience 2020Quote: ... two pairs of sgRNA vectors and Cas9 nickase plasmid were transduced into the AD hPSCs by using Human Stem Cell Nucleofector® Kit 1 (Lonza VPH-5012). Three days after electroporation ...
-
bioRxiv - Neuroscience 2021Quote: ... 1 × 106 cells were resuspended in 100 μl of the nucleofection hESC solution 1 (Human Stem Cell Nucleofector® Kit 1/ Lonza #VPH-5012) where 5 μg of plasmids were added previously ...
-
bioRxiv - Immunology 2020Quote: ... Human buffy coats were initially added to 40 ml chemical-defined serum-free culture X-VIVO 15™ mediums (Lonza, Walkersville, MD, USA) and mixed thoroughly with 10ml pipette ...
-
bioRxiv - Immunology 2022Quote: ... Activated cells were nuclofected with 100 µM AhR or non-targeting (NT1) siRNA (ON-TARGETplus SMART pool, Dharmacon) using the Amaxa Human T cell Nucleofector Kit (Lonza, Walkersville, MD, USA), according to the manufacturer’s protocol ...
-
bioRxiv - Cell Biology 2024Quote: The commercially available human myoblast (hMB) stock isolated from a healthy 35-year-old female (CC-2580, lot 0000483427; Lonza, Walkersville, MD, USA) was used [7,10] ...
-
bioRxiv - Microbiology 2023Quote: ... plates were incubated at 37°C for 30 minutes in blocking buffer (PBS with 1% BSA, 5% FBS, and 1% human AB serum (Lonza, Walkersville, MD, USA), washed three times in PBS with 0.05% tween 20 and incubated with a commercial anti-SARS-CoV-2 Spike antibody (1/1000 ...
-
bioRxiv - Immunology 2019Quote: ... the ribonucleoprotein (RNP) complex was first formed by incubating 10μg of TrueCut Cas9 Protein V2 (Lonza) with 5μg of sgRNA for 10-30min at room temperature ...
-
bioRxiv - Plant Biology 2020Quote: Ubiquitinated GST-SYP61 proteins were separated by SDS-PAGE and stained with SYPRO Ruby (Lonza, Switzerland) as described in the manufacturer’s protocol ...
-
bioRxiv - Bioengineering 2023Quote: ... 2×109 Freestyle CHO-S cells were resuspended in 500ml ProCHO 4 Protein-free Medium (Lonza, Cat#BEBP12-029 ...
-
bioRxiv - Cell Biology 2023Quote: ... were cultured at 27°C in Insect-XPRESS protein-free insect medium with L-Glutamine (Lonza) in shaking suspension ...
-
bioRxiv - Immunology 2023Quote: ... a ribonucleoprotein (RNP) complex was first formed by incubating 5µg of TrueCut Cas9 Protein V2 (Lonza) with 10µg of sgRNA for 10 min at room temperature ...
-
bioRxiv - Neuroscience 2020Quote: ... 6 µg of total plasmid DNA (2 µg for each of the three episomal vectors) were electroporated into 5×10^5 fibroblasts with the Amaxaμ Human Dermal Fibroblast Nucleofectorμ Kit (Lonza, Basel, Switzerland; VDP-1001). The fibroblasts were further cultured for 6 days to allow them to recover ...
-
bioRxiv - Microbiology 2020Quote: ... The previously described human proximal tubular epithelial cell line HPT-1b[26] was maintained in renal epithelial cell growth media (Lonza, cat. no. CC-4127). To generate the HPT-1b-mCherry cell line ...
-
bioRxiv - Cancer Biology 2019Quote: ... and 3-4 hours later were transfected with 2 μg of pBabe KRAS WT and pBabe KRAS G12D or pBabe KRAS Q61H plasmid DNA by electroporation using the Amaxa® Human T Cell Nucleofector® Kit (Lonza, Basel, Switzerland) following the manufacturer’s protocol ...
-
bioRxiv - Bioengineering 2023Quote: Cells were cultured at 37°C and 5% CO2 in cell-specific media: primary human umbilical vascular endothelial cells (HUVECs, Lonza; up to passage 6) in EGM-2 (Lonza ...
-
bioRxiv - Neuroscience 2023Quote: ... 4−106 cells were pelleted at 300xg and then resuspended in 100 μL prewarmed nucleofector solution (Human Stem Cell Kit 1, Lonza, catalog no. VPH-5012); 4 μg of NGN2 plasmid and 1.5 μg each of 1R and 1L plasmids were added directly to the resuspended cells ...
-
bioRxiv - Bioengineering 2023Quote: ... Complete growth medium for culturing of human adipose derived stem cells (ASCs, ATCC SCRC-4000) consists of Lonza ADSC Basal Medium (Lonza, Lexington, MA PT-3273), 10% MSC FBS (ThermoFisher Scientific) ...
-
bioRxiv - Molecular Biology 2019Quote: ... the total protein was size-separated on an 8% polyacrylamide gel and stained with SYPRO Ruby (Lonza). When the contents of the protein preparation were analyzed by western blotting ...
-
bioRxiv - Cell Biology 2023Quote: ... Sf21 cells were grown as a monolayer culture in Insect-XPRESS Protein-free Insect Cell Medium (Lonza) in standard T175 culture flasks at 27 °C ...
-
bioRxiv - Genomics 2021Quote: ... were thawed onto a 60 mm tissue-treated culture dish seeded with irradiated mouse embryonic fibroblasts (MEFs) as feeders in DMEM high glucose base medium supplemented with 15% fetal bovine serum (FBS, Lonza cat. no. 14-501F lot no. 0000217266), 1X Pen/Strep ...
-
bioRxiv - Immunology 2021Quote: Primary normal human bronchial epithelial cells (NHBECs) were obtained from a 65 year old Caucasian male without identifiers sourced from Lonza (NHBE CC-2540; Walkersville, MD). The NHBECs were revived from cryopreservation and expanded with PneumaCult™ Ex-Plus media (StemCell #05040 ...
-
bioRxiv - Cell Biology 2024Quote: Human primary lung microvascular endothelial cells (LMVECs) and pulmonary artery endothelial cells (PAECs) from different donors were purchased from Lonza (Walkersville, MD. See Table S1). Cells were seeded onto plates and cultured in complete medium comprising of endothelial basal medium-2 (EBM-2 ...
-
bioRxiv - Biophysics 2019Quote: ... 1 L of Sf9 insect cells in Insect-XPRESS Protein-free Insect Cell Medium with L-glutamine (Lonza) in a 3 L flask was infected with 5 ml of the harvested P2 (second passage ...
-
bioRxiv - Cancer Biology 2020Quote: CRISPR/Cas9 genome editing was performed by ribonucleotide-protein (RNP) nucleofection using the 4D-Nucleofector™ System (Lonza), following the electroporation conditions already optimized in the laboratory ...
-
bioRxiv - Immunology 2022Quote: ... used for baculovirus preparation were maintained in Insect-XPRESSTM Protein-free Insect Cell Medium with L-Glutamine (Lonza) at 28°C under agitation ...
-
bioRxiv - Cell Biology 2023Quote: ... with a blue fluorescent protein (BFP)-tag were cultured in smooth muscle cell growth medium-2 (SMGM2) (Lonza). Human umbilical cord vein endothelial cells (HUVEC ...
-
bioRxiv - Physiology 2024Quote: ... Proteins were separated by electrophoresis (ProSieve EX Running buffer 10x, diluted in H2O to 1x, (Lonza, Basel, CH); Precision Plus Protein WesternC Standards ...
-
bioRxiv - Cell Biology 2020Quote: ... MDCK II cells stably expressing these proteins were generated by transfection of 1.0×106 cells with Amaxa Nucleofector II (Lonza) using the L-005 program ...
-
bioRxiv - Immunology 2019Quote: ... Endotoxin concentrations were measured in all protein samples using the Limulus Amebocyte Lysate Kit – QCL-1000 (Lonza, Basel, Switzerland). The rMIC1 ...
-
bioRxiv - Cell Biology 2023Quote: ... RNP were formed by mixing 5 to 9 μgr base editor protein with 1.5 μgr of sgRNA in 20 μL of P3 buffer (Lonza, Amaxa P3 Primary Cell 4D-Nucleofector Kit ...
-
bioRxiv - Pharmacology and Toxicology 2021Quote: Human umbilical vein endothelial cells (HUVEC) cells were grown at 37° C in the Endothelial cell basal medium-2 (LONZA #Cat No-CC-3156 and CC-4176) under the atmosphere containing 5% CO2 ...
-
bioRxiv - Biochemistry 2019Quote: ... Sf21 cells (2 × 106 cells mL−1) were infected with the P3 viral stock in Insect-XPRESS protein-free medium (Lonza). The infected insect cells were grown at 27 °C with shaking for 66 hours ...
-
bioRxiv - Microbiology 2020Quote: ... major promastigotes expressing Cas9 and T7 RNA polymerase proteins [30] were co-transfected with donor DNA and sgRNA templates using V-033 program of Amaxa Nucleofector System (Lonza). Transfected parasites were selected with 50 μg/ml neomycin and cloned in semi-solid culture medium ...
-
bioRxiv - Microbiology 2020Quote: ... cells were transfected with pcDNA3 HA eIF4GI plasmid or control green fluorescent protein (GFP)-expressing plasmid (pMAX-GFP plasmid, Lonza). pcDNA3 HA eIF4GI (1–1599 ...
-
bioRxiv - Bioengineering 2021Quote: Primary human umbilical vein endothelial cells (HUVECs) and red fluorescent protein-expressing HUVECs (RFP-HUVECs) were maintained in EMG-2 medium (complete EGM-2 BulletKitTM, Lonza). We obtained human umbilical cords from Xinhua Hospital Affiliated to Shanghai Jiaotong University School of Medicine ...
-
bioRxiv - Cell Biology 2020Quote: ... The purity of CyaA batches is higher than 90% as judged by SDS PAGE analysis and contained less than 1 EU of LPS/μg of protein as determined by a standard LAL assay (Lonza). Finally ...
-
bioRxiv - Cell Biology 2021Quote: ... Western blots were conducted by running equivalent amounts of protein on 4-20% Tris-Glycine SDS/polyacrylamide gradient gels (Lonza) after reduction with 2-mercaptoethanol and heating to 95°C for 5m ...
-
bioRxiv - Immunology 2021Quote: ... for RNP formation 180 pmol crRNA-tracrRNA duplex was mixed with 60 pmol Cas9 protein in Nucleofection Buffer P3 (Lonza) and incubated for at least 10 min at room temperature ...
-
bioRxiv - Developmental Biology 2022Quote: ... This cocktail was provided to the cells together with a ribonucleoprotein complex consisting of crRNA, tracrRNA and Cas9 protein (IDT, refer to “Oligonucleotides” for crRNA sequence) via nucleofection using a 4D nucleofector (Lonza). Next ...
-
bioRxiv - Immunology 2024Quote: ... The reaction contained 400 pmol of Cas9 protein and 1 × 107 of the transduced NK cells in 100 µL of P3 solution (Lonza). Immediately after electroporation ...
-
bioRxiv - Molecular Biology 2023Quote: ... complexes for each peak to be deleted were prepared individually by mixing 120 pmol of sgRNA with 20 pmol of Cas9 protein (QB3 MacroLab, University of California, Berkeley) in 5 ul of P3 primary cell nucleofection buffer (Lonza) and incubated at room temperature for 15 minutes ...
-
bioRxiv - Physiology 2023Quote: ... 2 μl of KCNQ2/3 cDNA plasmid (0.9 g/l) and 1 μl of pmax green fluorescent protein vector (0.5 g/l) (Lonza, Cologne, Germany) were diluted in 125 μl of Opti-MEM medium (Life Technologies) ...
-
bioRxiv - Cell Biology 2023Quote: ... and 2000 p mol of a degron-tagged control protein (mTagBFP2-RxxG) in a 100 µl nucleofection reaction (4D nucleofector kit SE plus supplement SF1, Lonza) 14 hours following nucleofection ...