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Citations for Lonza :
1101 - 1150 of 2167 citations for 1 5 Bis 2 2 methyl 1 oxoallyl oxy ethyl dihydrogen benzene 1 2 4 5 tetracarboxylate since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2023Quote: ... spanning the whole SARS-CoV-2 genome was transfected into 2×106 HEK293T cells using the SF Cell Line 4D-Nucleofector-X Kit (Cat# V4XC-2012, Lonza) and the 4D-Nucleofector X Unit (Lonza ...
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bioRxiv - Immunology 2023Quote: ... Mixtures of complexed sgRNA and Cas9 (0.3 nmol synthetic sgRNA + 62 µmol Cas9 nuclease) and 2-10×106 enriched murine CD8+ T cells were suspended in 25ul of P3 electroporation buffer (Lonza) and electroporated using the Lonza 4D Nucleofector (pulse code DN100) ...
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bioRxiv - Bioengineering 2022Quote: ... 30 µl was mixed with 6 µl 6X gel loading dye and run on a 50 ml gel containing 2% SeaKem Agarose (Lonza), 1x Tris-Acetate-EDTA (Boston BioProducts) ...
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bioRxiv - Bioengineering 2022Quote: ... Bone marrow mononuclear cells were plated in endothelial growth medium 2 + 10% fetal bovine serum (FBS) (EGM-2MV, Lonza, Switzerland) on fibronectin coated plates and cultured at 37°C in 5% CO2 incubators ...
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bioRxiv - Cell Biology 2022Quote: ... Three slices were placed in one well of a 12-well plate and incubated with 2 mL DMEM (Lonza, #42430) with 0.6% amphotericin B ...
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bioRxiv - Systems Biology 2022Quote: ... The red blood cell lysis was performed by resuspending the pellet in 2 ml sterile ACK (Ammonium-Chloride-Potassium) lysis buffer (ACK Lysing Buffer, Lonza) and incubating on ice for 5 minutes ...
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bioRxiv - Bioengineering 2023Quote: ... washed three times in PBS and cultured on collagen-coated 6-well plates in endothelial growth medium (EGM-2, Lonza) composed of endothelial basal medium supplemented with 2% fetal bovine serum ...
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bioRxiv - Genomics 2022Quote: ... cells (from two donors) were grown to 80% to 90% confluence in endothelial basal medium 2-MV with supplements (EBM; Lonza) and 5% fetal bovine serum (FBS ...
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bioRxiv - Cell Biology 2023Quote: ... Five million BJAB or OCI-LY1 cells were transfected with 2 μg plasmid DNA using the SF Cell Line 4D-Nucleofector X Kit L (Lonza) and the AMAXA Nucleofector biosystem (BJAB ...
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bioRxiv - Developmental Biology 2024Quote: ... we proceeded with 50% Basal media (+10uh/ml HGF) and 50% SkGM™-2 Skeletal Muscle Cell Growth Medium (Lonza). Subsequently ...
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bioRxiv - Developmental Biology 2024Quote: ... A total of 2 × 104 cells were seeded in each well with 150 μl EC specific media (Lonza, CC-3202). TIFF images of capillary-like networks were captured using a Zeiss Axio Image M2 microscope equipped with a digital camera at 4 ...
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bioRxiv - Molecular Biology 2024Quote: ... resuspended in RDM to OD600 of ≈0.003 and several 1-µl drops of the diluted cell suspension was placed on an agarose pad prepared with RDM and 2% agarose (SeaPlaque GTG Agarose, Lonza). The agarose pad was surrounded with a gene frame (Thermo Fisher ...
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bioRxiv - Immunology 2024Quote: ... siRNAs were added to 2 µM and electroporation was performed using the program U-001 on the 3D-Nucleofector (Lonza). The siRNAs targeting Clever-1 ...
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bioRxiv - Microbiology 2024Quote: ... and 2.5µg of each of the sgRNAs were incubated together for at least 10 minutes at room temperature before nucleofection of iPSC cells occurred using a Human Stem Cell Nucleofector Kit 2 (Lonza). Cells were then plated at 25 cell/cm2 on 2x Cultrex-coated plates in mTeSR-Plus media with 1× CloneR supplement (STEMCELL Technologies) ...
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bioRxiv - Immunology 2024Quote: ... 1-2*106 T-ALL cells were resuspended in 100uL P3 nucleofector solution (82 uL nucleofection solution + 18 uL supplement; Lonza P3 Primary Cell 4D-NucleofectorTM X Kit) ...
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bioRxiv - Evolutionary Biology 2024Quote: ... 4 µL SpCas9/gRNA RNP + 2 µL repair template (2 to 8 µg) + 2 µL primed cells (4E6 cells) + 10 µg pUC19 plasmid (used as carrier DNA) + SF buffer (Lonza) to a final volume of 24 µL ...
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bioRxiv - Cell Biology 2024Quote: ... 100 µL aliquots (7 × 106 cells) were then transferred into 2 mm EP cuvettes and electroporated on an Amaxa Nucleofector I (Lonza). For A2780 cells ...
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bioRxiv - Bioengineering 2024Quote: ... Unlabelled HUVECs or HUVEC with a constitutive green fluorescent protein (GFP) reporter (CMV-GFP HUVEC, ATCC) were cultured in EGM-2 Bulletkit medium (Lonza) and used before 10 passages ...
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bioRxiv - Neuroscience 2024Quote: ... The CD146-positive cells remaining in the column were collected and cultured on collagen type I-coated dishes in in endothelial basal medium (EBM-2, Lonza) supplemented with endothelial growth factors EGM-2 SingleQuots (Lonza).
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bioRxiv - Cancer Biology 2024Quote: Whole bone marrow cells were obtained by flushing one femur with PBS 2% FBS followed by erythrocyte elimination with ACK Lysing Buffer (10-548E, BioWhittaker, Lonza). The remaining cells were filtered through 40 μm pore size cell strainer and counted ...
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bioRxiv - Cancer Biology 2024Quote: Bone marrow cells were obtained by flushing two femurs of each NSG mouse with 2% FBS in PBS followed by erythrocyte elimination with ACK Lysing Buffer (10-548E, BioWhittaker, Lonza). Cells filtered through 40 μm strainer were counted and 1×106 cells/well were stained with Live/Dead Blue (Thermo ...
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bioRxiv - Bioengineering 2024Quote: ... were transduced with a lentivirus to express tdTomato fluorescent protein (Vectorbuilder) and were cultured in EGM-2 media (Promocell or Lonza). Human astrocytes (Sciencell ...
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bioRxiv - Biophysics 2021Quote: ... The WT and mutant genes were then amplified from these expression plasmids using the PCR primers 5’-GCGCTAGCAGCACCCATATGCCTGAG-3’and 5’-ACGAAGCTTTCAGTGGTGGTGGTGGT-3’and subcloned into the pMaxCloning vector (Lonza, VDC-1040) via NheI and BamHI sites to generate the pMax_WT_NEIL1 and pMax_Mutant_NEIL1 expression plasmids that were utilized throughout this analysis.
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bioRxiv - Immunology 2021Quote: ... suspension CHO cells expressing wildtype human CTLA-4 were plated at 1 × 106 cells/well in DPBS (Lonza, Basel, Switzerland) containing 0.5% BSA (MilliporeSigma ...
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bioRxiv - Microbiology 2020Quote: ... The same number of cells (2 x 106 cells) were lysed in 100 µl of 1% CHAPS/PBS and analyzed on 4-20% SDS-PAGE gel (Lonza). The envelope proteins were detected by 2F5 (specific for gp41 ...
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bioRxiv - Cell Biology 2024Quote: ... Cells were passaged 1:10 every 3-4 days by trypsinisation and were regularly screened for mycoplasma contamination (Mycoalert, Lonza).
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bioRxiv - Neuroscience 2024Quote: ... along with 20 μg recombinant Cas9 (IDT) and 1 pmol of each bridging oligonucleotide using the P3 Primary Cell 4-D-Nucleofector kit (Lonza) using the CA-137 program34 ...
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bioRxiv - Microbiology 2021Quote: ... treated with 5 ml of ACK lysing buffer (Lonza), and frozen with 3ml of Bambanker freezing media ...
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bioRxiv - Cell Biology 2022Quote: ... 5 μL of 200× SYBR Green I (Lonza, Switzerland) was added to 200 μL of the diluted culture for a final concentration of 5× SYBR Green I ...
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bioRxiv - Microbiology 2021Quote: ... treated with 5 ml of ACK lysing buffer (Lonza), and frozen with 3ml of Bambanker freezing media ...
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bioRxiv - Cancer Biology 2023Quote: ... incubated with 5 ml of 0.5 mM EDTA (Lonza) at 37° C for 2 minutes to detach the cells ...
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bioRxiv - Microbiology 2022Quote: ... treated with 5 ml of ACK lysing buffer (Lonza), and frozen with 3 ml of Bambanker freezing media ...
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bioRxiv - Bioengineering 2023Quote: ... 1X (5 mL) of L-glutamine (Lonza, 17-605E), and 1X (5 mL ...
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bioRxiv - Physiology 2024Quote: ... containing microvascular SingleQuots Supplement Pack in 5% FBS (Lonza). Cells were studied after 12-14 days of differentiation at 37°C and were free of Mycoplasma infection ...
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bioRxiv - Physiology 2024Quote: ... supplemented with 5% fetal bovine serum (Lonza, Basel, Switzerland) at 37°C in a 5% CO2 atmosphere ...
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bioRxiv - Immunology 2022Quote: ... the apical medium was removed and the basal medium was changed to 1:1 BEBM (Lonza) with DMEM (Gibco) ...
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bioRxiv - Neuroscience 2023Quote: ... supplemented with neuronal survival factor-1 (NSF-1) (Lonza, Charles City, IA, USA, Cat# CC-4323), N2 supplement (Invitrogen ...
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bioRxiv - Cell Biology 2023Quote: ... Human SH-SY5Y dopaminergic neuroblastoma cells were cultured in Dulbecco’s modified Eagle’s medium:F-12 (DMEM:F-12; 1:1) containing high glucose (3.151 g/L) (Lonza), supplemented with 10% (v/v ...
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bioRxiv - Microbiology 2024Quote: ... 1 μL of which was spotted onto a 1% agarose PYE pad (SeaKem LE, Lonza Bioscience). The agarose pad was sandwiched between glass coverslips for imaging ...
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bioRxiv - Immunology 2022Quote: ... 1 × Penicillin/Streptomycin (Lonza, 17-602E) and centrifuged to pellet ...
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bioRxiv - Neuroscience 2021Quote: ... with 1% Penicillin-Streptomycin (Lonza, Switzerland) plus 10% FBS for primary microglia ...
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bioRxiv - Immunology 2021Quote: ... and 1% penicillin-streptomycin antibiotics (Lonza) and mycoplasma free (Mycozap antibiotics ...
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bioRxiv - Biochemistry 2020Quote: ... 1% non-essential amino acids (Lonza) and 2 mM L-glutamine ...
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bioRxiv - Bioengineering 2020Quote: ... 1 mM L-Glutamine (Lonza, 17605E), 100 μM MEM-Non-Essential Amino Acid solution (NEAA ...
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bioRxiv - Genetics 2022Quote: ... A 1% SeaKem agarose gel (Lonza) was poured in 0.25 X TBE buffer ...
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bioRxiv - Microbiology 2022Quote: ... non-essential amino acids (1×, Lonza), penicillin (100 IU/mL) ...
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bioRxiv - Cancer Biology 2021Quote: ... 1% Penicillin-Streptomycin (Lonza, 17-745E), 10% Fetal Bovine Sera (F4135 ...
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bioRxiv - Immunology 2022Quote: ... 1% sodium pyruvate (Lonza, Walkersville, MD), 1% nonessential amino acids (Lonza ...
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bioRxiv - Immunology 2022Quote: ... and 1× Mycozap (Lonza, VZA-2021). The cell suspension was seeded at a density of 50-100 MBCs and 3000 irr-3T3s per well of 384-well plates and co-cultured for 10 days to allow for MBC expansion and activation of Ig secretion ...
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bioRxiv - Synthetic Biology 2020Quote: ... and 1 mM sodium pyruvate (Lonza). Polyethylenimine (PEI ...