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Citations for Lonza :
1051 - 1072 of 1072 citations for PRG2 Protein Human Recombinant His Tag HPLC verified since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2023Quote: ... 1.5-2.0 x 105 cells were electroporated with 200 pmol of protein in 20 µl cuvettes using a 4D nucleofector device (Lonza) according to the manufacturer’s recommendations ...
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bioRxiv - Physiology 2023Quote: ... 2 μl of KCNQ2/3 cDNA plasmid (0.9 g/l) and 1 μl of pmax green fluorescent protein vector (0.5 g/l) (Lonza, Cologne, Germany) were diluted in 125 μl of Opti-MEM medium (Life Technologies) ...
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bioRxiv - Cell Biology 2024Quote: ... with a density of 1.5 x106 using a 1:70 inoculation from the V1 stock for 92 hours at 21°C in Insect Xpress Protein-free Insect Cell Medium (Lonza) supplemented with GlutaMAX (GIBCO ...
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bioRxiv - Cancer Biology 2024Quote: ... were annealed to Cas9 protein (IDT) and the ribonucleoprotein complex was transfected into SKOV-3 cells using a 4D-Nucleofector (Lonza). Bulk transfected cells were checked by flow cytometry for successful knock-down ...
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bioRxiv - Immunology 2024Quote: ... 2 × 106 NK cells were electroporated with 40 pmol of purified Cas9-NLS protein (MacroLab) complexed with 80 pmol of sgRNA (Synthego) using a 4D nucleofector (Lonza) in 20 μl of P3 buffer and supplement (Lonza ...
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bioRxiv - Bioengineering 2024Quote: ... were transduced with a lentivirus to express tdTomato fluorescent protein (Vectorbuilder) and were cultured in EGM-2 media (Promocell or Lonza). Human astrocytes (Sciencell ...
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bioRxiv - Bioengineering 2024Quote: ... Unlabelled HUVECs or HUVEC with a constitutive green fluorescent protein (GFP) reporter (CMV-GFP HUVEC, ATCC) were cultured in EGM-2 Bulletkit medium (Lonza) and used before 10 passages ...
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bioRxiv - Pathology 2020Quote: ... The purity of the final recombinant proteins was determined to be more than 99% by sodium dodecyl sulfate and polyacrylamide gel (SDS-PAGE) with an endotoxin concentration lower than 2 units/mg protein measured by Limulus Amebocyte Lysate PYROGENT™ 125 Plus (Lonza). In previous experiments we demonstrated that these recombinant proteins were functionally devoid of significant endotoxin contamination ...
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bioRxiv - Immunology 2020Quote: ... Six micrograms of plasmid DNA encoding the cDNA of the desired proteins was used for a single transfection reaction using program T-019 on the Amaxa Nucleofector II (Lonza, MD).
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bioRxiv - Neuroscience 2023Quote: ... TG trigeminal ganglion neurons were transfected with 3 µg of NaV1.7-PEPCy3 plasmid and 0.75 µg green fluorescent protein (GFP) using the rat neuron 4D-Nucleofector solution (P3 Primary Cell Solution, program DR 114; Lonza Biosciences). Neurons were plated on round bottom glass dishes (cat ...
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bioRxiv - Immunology 2024Quote: ... all from Dharmacon) complexed with TrueCut Cas9 Protein v2 using the SE Cell Line kit and 4D-Nucleofector X Unit (Lonza, RRID:SCR_023155) according to manufacturer’s protocol.
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bioRxiv - Neuroscience 2020Quote: ... Single iPSCs were then nucleofected with the pX459v2 plasmid coding for the Cas9 protein and the sgRNA using P3 primary Cell 4D nucleofector kit (Lonza, V4XP-302). After nucleofection cells were plated on a 6-well plate in E8 flex medium supplemented with RevitaCell ...
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bioRxiv - Immunology 2023Quote: ... The levels of endotoxin in the purified protein samples were assessed using the QCL-1000 Limulus amebocyte lysate system (Lonza, Basel, Switzerland), and it was found that the recombinant proteins had endotoxin levels of ∼ 4 pg/µg ...
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bioRxiv - Systems Biology 2024Quote: ... HiFi Cas9 Nuclease V3 protein (IDT, 1081058) were introduced into low-passage dual-reporter ESCs using the Mouse Embyronic Stem Cell Nucleofector Kit (Lonza VPH-1001). ESCs were treated with 0.025% trypsin/1% EDTA (Gibco ...
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bioRxiv - Immunology 2024Quote: ... sgRNAs targeting Mavs and NLSx2-Cas9 protein were mixed with FLAMs in P2 primary cell nucleofection solution (Lonza, catalog no. V4XP-2024). Nucleofection was carried out using the 4D-Nucleofector® Core and X Unit (Lonza ...
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bioRxiv - Pathology 2020Quote: ... The purity of the final recombinant proteins was more than 99% with an endotoxin concentration lower than 2 units/mg protein by Limulus Amebocyte Lysate PYROGENT™ 125 Plus (Lonza; Walkersville, MD).
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bioRxiv - Immunology 2024Quote: ... were incubated with sgRNA and CAS9 protein complex and electroporation was done using P3 Primary Cell 4D-NucleofectorTM X Kit L (Lonza, cat # V4XP-3024) and Lonza 4D nucleofector system ...
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bioRxiv - Immunology 2020Quote: ... 1.3×10^5 C25cl48 cells/reaction were electroporated with a ribonucleoprotein consisting of 125 pmol TrueCut Cas9 Protein v2 (A36498) + 150 pmol gRNA + 120 pmol ssDNA using P5 Primary Cell 4D-Nucleofector X Kit S (Lonza, Walkersville, MD, V4XP-5032) in a 16-well Nucleocuvette Strip and the Amaxa 4D-Nucleofector X Unit (Lonza ...
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bioRxiv - Biochemistry 2022Quote: All proteins were expressed using the baculoviral expression system and the lepidopteran High Five cell line in Insect-XPRESS (Lonza, cat. no. BELN12-730Q). Baculoviruses were produced using homologous recombination in insect cells by co-transfection with linearized baculoviral DNA and the transfer vector pAcGP67A (BD Biosciences) ...
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bioRxiv - Genetics 2023Quote: ... of sg1617 or sg1618 was mixed with 500 pmol (5 μM) of 3xNLS-SpCas9-SpCas9 protein and added electroporation buffer (Lonza 4D, cat# V4XP-3024) up to 25 μl in one tube ...
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bioRxiv - Genetics 2023Quote: ... of sg1617 or sg1618 (see sequences of sgRNAs in Table S2) was mixed with 100 pmol (5 μM) of 3xNLS-SpCas9 protein and added electroporation buffer (Lonza 4D, cat# V4XP-3032) up to 5 μl in one tube ...
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bioRxiv - Genomics 2024Quote: ... 300 pmol (15 μM) of sg1617 was mixed with 100 pmol (5 μM) of 3xNLS-SpCas9-HiFi protein and added electroporation buffer (Lonza 4D, cat# V4XP-3032) up to 5 μl in one tube ...