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Citations for Lonza :
1001 - 1050 of 1092 citations for Porcine Circovirus 2 PCV2 Protein since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2024Quote: ... The mixture was transferred to a confocal plate and subsequently UV crosslinked at 15 mW/cm2 for 1 minute and cultured for 4 days in EGM-2 media (Lonza).
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bioRxiv - Cell Biology 2024Quote: PC-3 cells (1 × 106 cells) were transfected with 2 μg of mGFP-GPI cDNA using a 4-D nucleofector (LONZA) and cultured in two 150-mm dishes until reaching approximately 100% confluence (2 × 107 cells) ...
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bioRxiv - Immunology 2024Quote: ... were incubated with sgRNA and CAS9 protein complex and electroporation was done using P3 Primary Cell 4D-NucleofectorTM X Kit L (Lonza, cat # V4XP-3024) and Lonza 4D nucleofector system ...
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bioRxiv - Synthetic Biology 2021Quote: ... and γδ T cells were activated at 1 x 106 cells mL-1 for 2 days in complete XVivo15 medium (Lonza) (5% fetal bovine serum ...
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bioRxiv - Molecular Biology 2021Quote: ... Cells were electroporated with enhancer (IDT, #1075915, final concentration 4 μM) in a nucleofector device (Lonza, Nucleofector 2; program D-032). Cells were incubated for 24 h to allow them to recover and then detached and cloned by serial dilution ...
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bioRxiv - Immunology 2022Quote: ... The concentration of IL-2 was increased to 1000 IU/ml on day 7 and the expanded T cells were electroporated with the indicated mRNA at a concentration of 2 pg mRNA/cell on day 8 using 4D Nucleofector™ System (Lonza) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: ... pcDNA3-JUP or empty vector were transfected into Caco-2 cells using SE Cell Line 4D-Nucleofector™ X Kit (Lonza) and an Amaxa 4D Nucleofector device from Lonza ...
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bioRxiv - Cancer Biology 2021Quote: ... expressing the human CD133 antigen45 was co-delivered with a PBase expression vector (2.5 μg) into 2 ⨯ 106 U251 cells using Nucleofector 2b (Lonza, Köln, Germany), followed by puromycin (500 ng/ml ...
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bioRxiv - Bioengineering 2020Quote: 2 million UCB-MNC/mL were cultured in X-VIVO 15 serum-free cell-culture medium (Lonza Group Ltd, Basel, Switzerland), supplemented with 0.5 μg/mL of FMS-like tyrosine kinase-3 and 0.5 μg/mL of stem-cell factor ...
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bioRxiv - Cell Biology 2022Quote: ... Cells were maintained at 37°C in 5% CO2 in either EGM-2 MV complete media (CC-3125, Lonza, Walkersville, MD) or in M199 supplemented with 20% FBS (Atlanta Biologicals ...
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bioRxiv - Bioengineering 2022Quote: ... the basal channels of the Intestine Chips were first rinsed with endothelial cell culture medium consisting of stem cell medium supplemented with components from EGM™-2 MV Microvascular Endothelial SingleQuots Kit (CC-4147; Lonza), and then 50 μL HIMECS (450,000 cells/chip ...
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bioRxiv - Genomics 2021Quote: ... The medium was changed to fresh fibroblast medium containing β-mercaptoethanol on day 2 and to a defined hepatocyte growth medium (HCM, Lonza) on day On day 6 ...
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bioRxiv - Developmental Biology 2022Quote: ... Days 5-10 (hepatoblast differentiation/ Stage 2) switched to complete Hepatocyte Culture Medium (HCM) (consisting of HBM Basal Medium and HCM SingleQuot Supplements (Lonza, UK)) containing 20 ng/ ml BMP2 and 30 ng/ ml FGF2 (both Peprotech) ...
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bioRxiv - Immunology 2020Quote: ... The inoculum was then removed and replaced with 2 mL of complete DMEM medium with 1% SeaPlaque agarose (Lonza, Cat# 50100). The plate was incubated at 37°C and 5% CO2 for 3 days ...
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bioRxiv - Biochemistry 2021Quote: ... 500,000 cells were seeded per well and 2 days later the experiment was started by replacing the culture medium with 2.5 ml Pro293a medium (Lonza, Basel, Switzerland), supplemented with 2 mM L-glutamine and 100 units pen/strep (Gibco ...
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bioRxiv - Cell Biology 2021Quote: ... and Subset Four (podoplanin+CD36+) were sorted into 5mL FACS tubes containing endothelial basal media (EGM-2 medium without supplements added, Lonza, USA) supplemented with 10% FBS at 4°C degrees ...
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bioRxiv - Immunology 2023Quote: ... vectors was performed on day 2 of activation into 1×106 T cells using 4D-Nucleofector according to the manufacturer’s instructions (Lonza, Cologne, Germany).
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bioRxiv - Physiology 2022Quote: ... A second enrichment with anti-CD102-coated Dynabeads was performed before culturing cells at 37Cº on fibronectin-coated tissue culture dishes in EGM-2 media (Lonza Walkersville). Cells (passage two ...
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bioRxiv - Bioengineering 2024Quote: ... HepG2 and K562 cells (2×105 cells/condition) were electroporated using the SF Cell Line 96-well Nucleofector™ Kit (Lonza) and the CM-130 ...
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bioRxiv - Bioengineering 2024Quote: ... with L-ascorbic acid-2-phosphate (AA2P) (5mg/ml stock, 10 µl/ml), and β-glycerolphosphate (BGP) (20 mM stock, 20 µl/ml) (Lonza). The cell culture medium was changed every 3 days ...
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bioRxiv - Neuroscience 2024Quote: ... digested for 3 min at 37 °C in Accutase and then resuspended in 60 μL nucleofection buffer from the Human Stem Cell Nucleofector™ Kit 2 (Lonza). The suspension was combined 2 μM Electroporation Enhancer (IDTDNA ...
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bioRxiv - Microbiology 2024Quote: ... Passage 2 cells were transferred to 75 mL flasks and grown in confluence in Dulbecco Modified Eagle medium (DMEM) (Lonza BioWhittaker) containing 10% heat-inactivated fetal bovine serum (Gibco ...
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bioRxiv - Immunology 2024Quote: ... and spun at 200xg for 10 min and resuspended at a concentration of 2-10x106 cells/100µL in Lonza Buffer P3/supplement (Lonza #V4XP-3024). The RNP complex and 100µL of resuspended cells were combined and electroporated using pulse code EO-115 on the Lonza 4D-Nucleofector Core/X Unit.
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bioRxiv - Molecular Biology 2024Quote: ... HSVECs were obtained by enzymatic collagenase digestion of human saphenous veins (Ethics 15/ES/0094) and maintained in EC growth medium (EGM-2 BulletKit™) (Lonza) supplemented with foetal bovine serum (10% ...
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bioRxiv - Microbiology 2024Quote: ... falciparum strains were cultured at 2% haematocrit in O+ erythrocytes and RPMI-1640 medium (Lonza, BE12-167F or Gibco, 31870-025) supplemented to give the following final concentrations ...
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bioRxiv - Physiology 2022Quote: ... (5 g/L trypsin and 2 g/L EDTA diluted 1 in 5 in calcium- and magnesium-free PBS) (Lonza, UK).
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bioRxiv - Cell Biology 2023Quote: ... Human visceral pre-adipocytes were purchased from Lonza (PT-5005) and cultured according to the manufacturer’s instructions in PGM-2TM Preadipocyte Growth Medium-2 BulletKitTM from Lonza (PT-8002). Once cells were expanded ...
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bioRxiv - Cancer Biology 2023Quote: ... HUVEC cells were maintained in growth factor-supplemented media (EBM + EGM-2 aliquots + 10% FBS) from Lonza (C-3121, CC-4542). MDA-MB-231 cell line was modified to express GFP (green fluorescent protein ...
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bioRxiv - Molecular Biology 2023Quote: ... Cells were infected with virus dilutions (from 10-1 to 10-9) prepared in DMEM supplemented with 2% FBS and 1%Penicillin/Streptomycin mixture (Lonza Biosciences). Infected cells were incubated at 37°C for 7 to 15 days ...
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bioRxiv - Cancer Biology 2024Quote: ... K562 and NALM6 cells (2×105 cells/transfection) were transfected using a Lonza 4D nucleofector™ with a SF nucleofection kit (Lonza) following manufacturer’s recommendations ...
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bioRxiv - Molecular Biology 2024Quote: ... 2 μL of the final 100 μM siRNA duplex was mixed with 100 μL of Nucleofection solution V (Lonza, VVCA-1003) and transfected into 4 million cells (48 h knockdown ...
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bioRxiv - Molecular Biology 2024Quote: ... 2 μL of the final 100 μM siRNA duplex was mixed with 100 μL of Nucleofection solution V (Lonza, VVCA-1003) and transfected into 10 million cells using a NucleofectorTM 2b Device and program T-029 (Lonza) ...
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bioRxiv - Biochemistry 2024Quote: ... Stable cell lines containing an empty vector or HA-labeled H2B variant were derived by electroporating ∼1 x 106 MCF10A cells and 2 µg of the minigene plasmid with a Lonza 4D-Nucleofector® X Unit (Lonza) and SF Cell Line 4D-Nucleofector™ X Kit S (Lonza ...
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bioRxiv - Bioengineering 2024Quote: ... Liver sinusoidal endothelial cells were isolated using CD146 microbeads (Miltenyi Bio 130-092-007) according to manufacturer’s instructions and cultured in EBM-2 media with supplements (Lonza CC-3162). LSECs were plated at 2×104 cells per well in 96-well tissue culture plates ...
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bioRxiv - Cell Biology 2024Quote: ... The reagents were mixed with 1 million cells resuspended in 100 μl human stem cell buffer kit 2 (Lonza, VCA-1005); the cells were electroporated using the Amaxa nucleofector IIb program B-016 ...
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bioRxiv - Immunology 2020Quote: ... 1.3×10^5 C25cl48 cells/reaction were electroporated with a ribonucleoprotein consisting of 125 pmol TrueCut Cas9 Protein v2 (A36498) + 150 pmol gRNA + 120 pmol ssDNA using P5 Primary Cell 4D-Nucleofector X Kit S (Lonza, Walkersville, MD, V4XP-5032) in a 16-well Nucleocuvette Strip and the Amaxa 4D-Nucleofector X Unit (Lonza ...
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bioRxiv - Genetics 2023Quote: ... of sg1617 or sg1618 was mixed with 500 pmol (5 μM) of 3xNLS-SpCas9-SpCas9 protein and added electroporation buffer (Lonza 4D, cat# V4XP-3024) up to 25 μl in one tube ...
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bioRxiv - Genetics 2023Quote: ... of sg1617 or sg1618 (see sequences of sgRNAs in Table S2) was mixed with 100 pmol (5 μM) of 3xNLS-SpCas9 protein and added electroporation buffer (Lonza 4D, cat# V4XP-3032) up to 5 μl in one tube ...
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bioRxiv - Biochemistry 2022Quote: All proteins were expressed using the baculoviral expression system and the lepidopteran High Five cell line in Insect-XPRESS (Lonza, cat. no. BELN12-730Q). Baculoviruses were produced using homologous recombination in insect cells by co-transfection with linearized baculoviral DNA and the transfer vector pAcGP67A (BD Biosciences) ...
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bioRxiv - Genomics 2024Quote: ... 300 pmol (15 μM) of sg1617 was mixed with 100 pmol (5 μM) of 3xNLS-SpCas9-HiFi protein and added electroporation buffer (Lonza 4D, cat# V4XP-3032) up to 5 μl in one tube ...
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bioRxiv - Cell Biology 2022Quote: ... and CD31+Rspo3+ cells were centrifuged at 500 g for 5 min and resuspended in 500 μl endothelial cell media: EGM-2 MV Bullet Kit (Lonza, cc-3202) supplemented with 50 ng/mL VEGF-C (R&D ...
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bioRxiv - Systems Biology 2020Quote: ... The pellets were resuspended in 10 mL of complete mammary epithelial growth medium with 2% v/v FBS without GA-1000 (MEGM; Lonza CC-3150). Cells were incubated at 37 °C for 1 h ...
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bioRxiv - Cell Biology 2021Quote: Genes were knocked out using 1-2 synthetic sgRNAs (Synthego) in complex with spCas9 (Aldeveron 9214) that were electroporated into cells using a nucleofector system (Lonza V4XC- 1032) according to published methods (Hoellerbauer et al. ...
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bioRxiv - Biophysics 2021Quote: All experiments using HUVEC were performed with an hTERT-immortalized HUVEC line previously described (28) and cultured in EGM-2 media (Lonza, CC-3162). For transient DNA transfections ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: HUVEC cells were grown at 37° C in the Endothelial cell basal medium-2 (LONZA #Cat No-CC-3156 and CC-4176) under the atmosphere containing 5% CO2 ...
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bioRxiv - Microbiology 2022Quote: ... 1.2E6 BHK21 cells were nucleofected with 2 µg of Spike plasmid using an Amaxa Nucleofector II with cell line kit L (Lonza #VCA-1005) and program A-031 ...
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bioRxiv - Systems Biology 2022Quote: ... Early passage HUVECs were cultured in EGM™2- Bulletkit™ medium with growth supplements CC-3156 & CC-4176 purchased from Lonza. At 80% confluency ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... Cells were maintained in RPMI 1640 medium supplemented with 2 mM Glutamine and 10% (v/v) heat-inactivated FBS (Lonza Group Ltd.) containing 100µg streptomycin ml−1 and 100 U penicillin ml−1 and passaged as per the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2021Quote: ... and CRX +/tdtomato were maintained in reduced growth factor Matrigel (50ug/cm^2) in mTeSR1 plus with 1x Pen/Strep Amphotericin B (Lonza, 17-745E).
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bioRxiv - Molecular Biology 2021Quote: ... Myoblasts derived from an adult DM1 patient biopsy (DM-05 (Xia et al., 2013)) were cultured in SkGM-2 skeletal muscle cell growth medium (Lonza, #CC-3245).