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Citations for Lonza :
51 - 100 of 4003 citations for Dengue Virus Serotype 4 NS1 Protein Insect Cells since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2023Quote: ... in Insect-XPRESS medium (Lonza, BELN12-730Q) medium at a cell density of 2.0□×□106 cells/ml for 64 to 70□h at 27□°C.
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bioRxiv - Biophysics 2023Quote: ... 150µl serum-free media (Insect-XPRESS, Lonza) was added to the 24 deep well plate ...
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bioRxiv - Biochemistry 2024Quote: ... in serum-free Insect-XPRESS medium (Lonza) were infected with recombinant virus (MOI>2 ...
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bioRxiv - Microbiology 2021Quote: The insect cell line Ld652Y (22) was maintained at 28°C in TC100 medium (Lonza) supplemented with 10% heat-inactivated fetal calf serum (Thermo scientific) ...
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bioRxiv - Developmental Biology 2022Quote: ... the cells were weaned off complete Schneider’s medium and into serum-free Insect-XPRESS (Lonza) for large-scale protein production ...
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bioRxiv - Biophysics 2023Quote: ... Sf9 cells were maintained as a suspension culture in serum-free medium (Insect-XPRESS, Lonza) at 27°C.
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bioRxiv - Cell Biology 2019Quote: ... in Grace’s insect media (Lonza, Walkersville, MD, USA)) into a 9-well glass plate using forceps ...
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bioRxiv - Biochemistry 2024Quote: ... grown in Insect-Xpress medium (Lonza, 12-730Q), as described previously35 ...
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bioRxiv - Evolutionary Biology 2022Quote: ... The cells (1.6 × 106 cells* ml) were grown in 50 ml of Insect-Xpress medium (Lonza, Walkersville, MD, USA) with 15 mg/ml gentamycin (Roth ...
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bioRxiv - Molecular Biology 2022Quote: Sf9 cells (used for generating baculovirus) were cultured in Insect-Xpress medium (Lonza, Cat#BELN12-730Q) supplemented with 0.1 % pluronic acid (Gibco ...
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bioRxiv - Cell Biology 2024Quote: ... Large scale D.Mel-2 cultures were prepared by diluting cells to a density of 106 cells/mL in Insect Xpress culture medium (Lonza) in Erlenmeyer flasks ...
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bioRxiv - Developmental Biology 2022Quote: ... ovaries were dissected in Grace’s insect medium (GIM) (Lonza) and fixed with 5.3% paraformaldehyde (PFA)/GIM for 13 min ...
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bioRxiv - Biochemistry 2020Quote: ... 20 mL overnight cultures (in Insect Xpress media (Lonza)) of selected clones were grown ...
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bioRxiv - Cell Biology 2022Quote: ... BUB-1 and CLS-2::GFP production was performed in 2 L SF9 cells in Insect-XPRESS (Lonza) medium (1×10^6 cells/mL) ...
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bioRxiv - Biochemistry 2023Quote: ... Briefly, D.mel-2 cells (CRL-1963, ATCC) were grown at 25°C in Insect-Xpress medium (181562, Lonza) supplemented with 1% Pen/Strep (15140122 ...
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bioRxiv - Bioengineering 2023Quote: ... 2×109 Freestyle CHO-S cells were resuspended in 500ml ProCHO 4 Protein-free Medium (Lonza, Cat#BEBP12-029 ...
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bioRxiv - Evolutionary Biology 2022Quote: ... and used to infect fresh Sf9 cells (1.2 × 106 cells* ml) in 10 ml of Insect-Xpress medium (Lonza, Walkersville, MD, USA) with 15 mg/ml gentamycin (Roth ...
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bioRxiv - Cell Biology 2023Quote: K562 cells stably expressing proinsulin [12] and HEK293T cells for virus production were cultured in IMDM (Lonza). All media were supplemented with 10% FCS ...
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bioRxiv - Microbiology 2022Quote: ... All cells and virus stocks tested negative for mycoplasma using MycoAlert Plus reagents (Lonza). Heat-inactivated serum samples were serially diluted in 3-fold steps in DMEM supplemented with 2% heat-inactivated fetal bovine serum (HI-FBS) ...
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bioRxiv - Biochemistry 2022Quote: ... S2 cells were selected at 27°C in complete Schneider’s medium and then transferred to Insect Xpress medium (Lonza) for large-scale expression in 2-liter Erlenmeyer flasks ...
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bioRxiv - Molecular Biology 2022Quote: ... The resulting vector was used to generate a D.mel-2 stable cell line adapted to grow in suspension in serum-free Insect-Xpress medium (Lonza). In brief ...
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bioRxiv - Genetics 2020Quote: ... intestines were dissected in ice-cold Grace’s Insect Medium (Lonza Bioscience) and fixed in a 1:1 (V/V ...
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bioRxiv - Biochemistry 2023Quote: ... in 450mL using Insect-XPRESS with L-Glutamine (Lonza LZBE12-730Q). For catalytic subunits ...
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bioRxiv - Molecular Biology 2021Quote: ... Bacmids were prepared by transposition of these plasmids and were used to transfect Sf9 cells in Insect Express media (Lonza) before viral amplification in the same cell line using standard techniques ...
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bioRxiv - Cell Biology 2019Quote: Whole-mount Drosophila ovary samples were dissected into Grace’s insect medium (Lonza, Walkersville ...
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bioRxiv - Bioengineering 2020Quote: ... For transfection 4 × 106 cells/mL cells were resuspended in ProCHO-4 Medium (Lonza) supplemented with 4 mM Ultraglutamine (Lonza) ...
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bioRxiv - Microbiology 2022Quote: ... The virus pellet was resuspended to EBM2 (Lonza) media ...
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bioRxiv - Biochemistry 2020Quote: ... Resulting viruses were used to infect Sf21 expression cultures in insect-Xpress media (Lonza). Expression culture cells were harvested 48 hours after proliferation arrest ...
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bioRxiv - Cell Biology 2019Quote: Approximately 5 whole ovary pairs were dissected in room temperature Grace’s insect media (Lonza, Walkersville ...
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bioRxiv - Cell Biology 2023Quote: Drosophila ovaries (5-8 pairs per sample) were dissected into room temperature Grace’s insect medium (Lonza). Ovaries were fixed for 10 min using 4% paraformaldehyde diluted in Grace’s medium ...
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bioRxiv - Bioengineering 2020Quote: Passage 4 human mesenchymal stem cells (Lonza, Switzerland) were expanded to passage 5 in an incubator set at 37°C and 5% CO2 and fed with low glucose DMEM and glutamine ...
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bioRxiv - Genomics 2022Quote: ... 4 million cells were electroporated (Amaxa Nucleofection, Lonza) with 25μg of L1-Cag/FLAG_NLS_ORF-1L plasmid and 15μg of pIC-Cre ...
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bioRxiv - Cancer Biology 2020Quote: ... CHO-S cells were resuspended at a density of 4 mio cells/mL in ProCHO 4 medium (Lonza, #LZ-BE12-029Q) supplemented with 8 mM ultraglutamine (Lonza ...
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bioRxiv - Cell Biology 2019Quote: ... Flies were then dissected at room temperature (RT) in Grace’s Insect Medium (modified) (BioWhittaker, Lonza, Cologne, Germany). Ovaries were homogenized in 150 uL of sample buffer and proteins were separated on 4-20% polyacrylamide gels using a standard SDS-PAGE protocol for Western blotting ...
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bioRxiv - Microbiology 2023Quote: ... Cells were co-incubated with virus and then supplemented with overlay medium that contains 1% SeaPlaque agarose (Lonza Bioscience, Basel, Switzerland). After 2 days of incubation ...
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bioRxiv - Microbiology 2022Quote: ... Virus was removed and 2 mL of overlay media (EMEM 1X Lonza 12-684F ...
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bioRxiv - Cancer Biology 2022Quote: ... NHEM-Ad cells were grown in MGM-4 Melanocyte Growth Medium-4 BulletKit (Lonza, Cat# CC-3249). We added penicillin (50 U/mL ...
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bioRxiv - Cell Biology 2019Quote: ... 3x 10 flies were then dissected at room temperature (RT) in Grace’s Insect Medium (modified) (BioWhittaker, Lonza, Cologne, Germany). Ovaries were fixed for 20 minutes in 4% paraformaldehyde and 20mM formic acid solution (Sigma ...
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bioRxiv - Cell Biology 2021Quote: Whole-mount Drosophila ovary samples (approximately 5 flies per experiment) were dissected into Grace’s insect media (Lonza, Walkersville, MD) and fixed for 10 minutes at room temperature in 4% paraformaldehyde in Grace’s insect media ...
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bioRxiv - Genetics 2019Quote: ... Between 1×10^4 – 5×10^4 cells per condition were resuspended in 20μl of Buffer P3 (Lonza) per reaction and quickly added to the Eppendorf tube containing the Cas9 gRNA RNP complex ...
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bioRxiv - Immunology 2019Quote: ... and primary T cells were electroporated using the P3 Primary Cell 4-D Kit (Lonza). For Cas9 and sgRNA delivery ...
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bioRxiv - Immunology 2023Quote: ... and primary T cells were electroporated using the P3 Primary Cell 4-D Kit (Lonza). For Cas9 and sgRNA delivery ...
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bioRxiv - Bioengineering 2023Quote: Primary human umbilical vein endothelial cells (HUVECs; Lonza; passages 4 to 7) were cultured on dishes in Endothelial Cell Growth Medium-2 (EGM-2 ...
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bioRxiv - Cell Biology 2021Quote: ... Western blots were conducted by running equivalent amounts of protein on 4-20% Tris-Glycine SDS/polyacrylamide gradient gels (Lonza) after reduction with 2-mercaptoethanol and heating to 95°C for 5m ...
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bioRxiv - Neuroscience 2023Quote: ... iPS cells were passaged every 3-4 days with Accutase (Lonza, Basel, Switzerland) and seeded on new plates with density 1:5 – 1:10 in iPS medium with ROCK inhibitor Y-27632 (Sigma-Aldrich) ...
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bioRxiv - Cell Biology 2024Quote: ... Cells were nucleofected with the 4-D Nucleofector System (Lonza, Cat#AAF-1003X) using the CA-137 program ...
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bioRxiv - Molecular Biology 2024Quote: ... 2-4 x 105 cells were resuspended in 100uL of P2 medium (Lonza) containing 10 µg of Cas9 or Cas9/guide plasmidic DNA and transferred to a cuvette (Lonza) ...
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bioRxiv - Biochemistry 2023Quote: ... Virus was concentrated using ultracentrifugation (95000 g for 3 hr) and suspended using OPTI-MEM (Lonza). Viral transfections assays were performed by plating target 293T (ACE2+ve ...
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bioRxiv - Cell Biology 2022Quote: ... The cell/DNA mixture was then nucleofected using the 4-D nucleofector system (Lonza) code CB-150 and densely replated on Matrigel coated 6-well plates at 2.5×106 cells per well ...
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bioRxiv - Immunology 2020Quote: ... Sorted B cells were cultured for 4 days in RPMI 1640 with UltraGlutamine (Lonza) containing 10% FBS (Dominique Dutscher) ...