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Citations for Lonza :
51 - 75 of 75 citations for Clostridium Difficile Toxin B since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2022Quote: ... NHBE cells were infected with viruses at an MOI of 0.01 and cultured in B-ALI growth medium (Lonza) at 33°C ...
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bioRxiv - Developmental Biology 2019Quote: ... were nucleofected into 1×106 pluripotent RUES2 cells using the B-016 setting on an Amaxa Nucleofector II (Lonza). Nucleofected cells were then plated as per maintenance conditions ...
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bioRxiv - Developmental Biology 2020Quote: ... Cells were nucleofected with 10ug lineage recorder DNA and 1ug Sleeping Beauty transposase following the manufacturer’s protocol and using the B-16 program of the Nucleofector 2b (Lonza) in cuvettes for 100μl Human Stem Cell nucleofection buffer (Lonza ...
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bioRxiv - Cell Biology 2021Quote: ... were grown in Dulbecco’s modified Eagle’s medium (DMEM) supplemented with 10% (v/v) fetal bovine serum (FBS) and 1X penicillin/streptomycin/amphotericin B (Lonza) at 37°C with 5% CO2 ...
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bioRxiv - Bioengineering 2023Quote: ... 0.1% r-human fibroblast growth factor (rhFGF) and 0.1% Gentamicin sulfate amphotericin-B (GA-1000) (Lonza, Quakertown, PA, USA). HLFs were used from passages 1 to 10 ...
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bioRxiv - Cancer Biology 2019Quote: ... Human cerebral microvascular endothelial cells (hCMEC/D3 [hCMEC]; gift from Dr. B. Weksler, Cornell Medical College) were grown in endothelial basal medium-2 (Lonza) supplemented with 5% FBS ...
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bioRxiv - Cell Biology 2022Quote: ... Expression of eGFP-Centrin1 was achieved by electroporating 4.106 B lymphoma cells with 4 μg of plasmid using the Amaxa Cell Line Nucleofector Kit R (T-016 program, Lonza). Cells were cultured in CLICK medium for 5 to 16 h before imaging.
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bioRxiv - Neuroscience 2022Quote: ... and 252 pmol Cas9-HiFi or Cpf1-Ultra (both IDT) using the B-16 program of the Nucleofector 2b Device (Lonza) in cuvettes for 100 µl Human Stem Cell nucleofection buffer (Lonza ...
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bioRxiv - Immunology 2022Quote: ... Knockdown of Vps4a/b was performed using Amaxa Nucleofector II and the Mouse T-Cell Nucleofector Kit (Lonza, Basel, Switzerland) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2019Quote: ... A20 and A20 D1.3 B cells (3 × 106cells) were transiently transfected using the AMAXA nucleofector kit V (Lonza, #VCA-1003) or the Ingenio electroporation kit (Mirus ...
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bioRxiv - Immunology 2021Quote: Spleens and cervical lymph nodes were isolated from 6-10 weeks old C57B6 mice and homogenized through a sieve in B cell culture medium (RPMI-1640 (Lonza), 10% fetal calf serum (FCS ...
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bioRxiv - Immunology 2022Quote: ... was prepared by mixing 250 nmol target gene sgRNA+ 50 nmol eGFP sgRNA+ 50 nmol SpCas9 2NLS Nuclease (Synthego) in 50 μL mouse B cell nucleofection buffer (Lonza) at RT for 30min ...
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bioRxiv - Cancer Biology 2023Quote: ... containing a combination of the px458 plasmid targeting each gene and the ssODN and then electroporated using B-016 program of Nucleofector 2b (Lonza). The electroporated GSCs were cultured for 48 h followed by Single cell sorting of GFP-positive cells (SH800 ...
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bioRxiv - Bioengineering 2022Quote: ... B and T cell electroporations were performed using the Amaxa™ 96-well Shuttle™ with the 4D Nucleofector (Lonza). HSPC electroporations were performed in MaxCyte ATx ...
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bioRxiv - Biochemistry 2022Quote: ... ALI culture conditions were initiated when cells reached confluence by removal of apical medium and replacement of basolateral growth medium with B-ALI differentiation medium (cat# 193517, Lonza) supplemented with SingleQuots (cat# 193515 ...
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bioRxiv - Neuroscience 2023Quote: ... we used the human iPSC line SFC840-03-01 (STBCi026-B) generated within the StemBANCC project (part of Innovative Medicines Initiative) based on a commercially available fibroblast line (Lonza) from a healthy 67-years old female donor (Morrison et al. ...
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bioRxiv - Cell Biology 2024Quote: ... Differentiation medium consisted of 50 % DMEM with pyruvate and 50 % BEBM supplemented with BEGM singlequots (except amphotericin B, triiodothyronine, and retinoic acid) (Lonza). Medium was supplemented with 100 nM all-trans retinoic acid (Sigma-Aldrich ...
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bioRxiv - Bioengineering 2024Quote: ... NHDF cells were cultured in FBM fibroblast basal media (supplemented with FGM-2 SingleQuot supplements: insulin, hEGF-B, GA-1000, and FBS) (Lonza). NHEK cells were expanded in KBM gold basal medium (KGM gold supplemented with Lonza’s SingleQuot supplements ...
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bioRxiv - Cell Biology 2021Quote: ... the media was changed to α-MEM containing macrophage colony-stimulating factor (M-CSF; 10 ng/ml) and and 66ng/ml receptor activator of nuclear factor kappa-B ligand (RANKL, Lonza Biosciences). OC activity was measured by releasing the europium conjugated human Type I collagen-coated on the bottom of the OsteoLyse™ Assay Kit (Lonza Biosciences ...
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bioRxiv - Immunology 2021Quote: ... The purified CD19+ B cells were cultured in the chemical-defined and serum-free X’VIVO 15 medium (Lonza, Walkersville, MD, USA), in the absence or presence of 100 U/ml penicillin ...
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bioRxiv - Systems Biology 2024Quote: ... MT2 and MDA-MB-231 cells with HLA-A,B,C CRISPR-Cas9 knockout were generated through electroporation of Cas9-sgRNA ribonucleoprotein (RNP) complex using 4D-Nucleofector (Lonza Bioscience). Three sgRNAs targeting HLA gene (HLA sgRNA#1 ...
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bioRxiv - Biochemistry 2022Quote: ... For dual sgRNA gene editing 100 pmols of Cas9 RNP with cut site A sgRNA and 100 pmols of Cas9 RNP with cut site B sgRNA were nucleofected (Lonza 4D nucleofector) with CA137 pulse code ...
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bioRxiv - Cell Biology 2021Quote: ... and CRX +/tdtomato were maintained in reduced growth factor Matrigel (50ug/cm^2) in mTeSR1 plus with 1x Pen/Strep Amphotericin B (Lonza, 17-745E).
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bioRxiv - Immunology 2020Quote: ... 1 × 106 BLaER1 cells were transfected with 2 μg plasmid DNA using the Human B Cell Nucleofector Kit and Nucleofector I device (program U-15; both Lonza, Basel, Schweiz). On day 2 post transfection ...
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bioRxiv - Bioengineering 2023Quote: ... MCF-10A cells were cultured using an MEGM™ Mammary Epithelial Cell Growth Medium BulletKit™ without gentamycin-amphotericin B mix (Lonza) following ATCC’s instructions ...