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Citations for Lonza :
51 - 100 of 155 citations for 6 6' Di O tert butyldimethylsilyl D lactal since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2020Quote: ... EBs were collected and transferred into 6-well cell culture plate (12-16 EBs/well) in X-VIVO15 (Lonza) supplemented with IL-3 ...
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bioRxiv - Bioengineering 2020Quote: ... Cells were routinely tested for mycoplasma every 6 months to ensure cell quality (MycoAlert™ Mycoplasma Detection Kit; Lonza).
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bioRxiv - Microbiology 2021Quote: ... Cells were tested for Mycoplasma contamination every 4 - 6 weeks and before each experiment (Mycoalert Mycoplasma Detection kit, Lonza).
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bioRxiv - Cancer Biology 2020Quote: ... were used in passages 3-6 and cultured on 0.1% gelatin-coated tissue culture plates in complete EGM2 (Lonza) supplemented to a total of 10% Fetal Bovine Serum (FBS) ...
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bioRxiv - Neuroscience 2020Quote: ... 4-6 μg of plasmid was electroporated using the AMAXA Nucleofector device (Neurons Rat DRG, G-013 program; Lonza) before plating and maintained for 48 h.
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bioRxiv - Systems Biology 2022Quote: DMECs were plated on poly-L-lysine-coated 6-well glass-bottom plates at 8,450 cells per cm2 in either BPM or growth medium (EGM2-MV, Lonza). After 24 hours ...
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bioRxiv - Cell Biology 2022Quote: Human umbilical vein endothelial cells (HUVEC) were cultured between passage 3 and 6 in EGM-2 complete medium (Lonza)3,52–55 ...
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bioRxiv - Neuroscience 2022Quote: Primary human astrocytes were cultured on Matrigel-coated (0.08 mg/well) 6-well plates in astrocyte growth medium (Lonza). On the day of plating ...
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Tuning trophoblast invasion in a gelatin hydrogel via soluble cues from the maternal-fetal interfacebioRxiv - Bioengineering 2020Quote: ... Routine mycoplasma testing was performed every 6 months to ensure cell quality using the MycoAlert™ Mycoplasma Detection Kit (Lonza).
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bioRxiv - Microbiology 2021Quote: ... Viral supernatants were collected 3 and 6 days post-infection and induced-cell death and viral titers were determined in the ToxiLight Bioassay (Lonza) and PFA ...
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bioRxiv - Cell Biology 2020Quote: ... were resuspended at a density of 6×106 cells mL−1 in Microvascular Endothelial Cell Growth Medium-2 media (EGM-2MV, Lonza). The bottom channel of the chip was washed with EGM-2MV and loaded with 6 μL of HIMEC cell suspension (~36,000 cells per chip) ...
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bioRxiv - Bioengineering 2022Quote: ... were used in passage 2 to 6 and were cultivated in Endothelial Cell Growth Medium-2 (EBM-2 medium supplemented with EGM-2 SingleQuots supplements, Lonza).
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bioRxiv - Bioengineering 2022Quote: Two donors of human mesenchymal stem cells (hMSCs) at passage 5-6 were seeded on mineralized collagen scaffolds for osteogenesis experiments (seeded separately, BM-17, Lonza, Maryland ...
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bioRxiv - Biophysics 2022Quote: ... 4-6 µg of DNA was mixed with the resuspended cells and electroporated using the Amaxa cell nucleofector (Lonza Laboratories) program Q-001 ...
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bioRxiv - Genomics 2022Quote: ... hiPSC-derived epicardial cells were dissociated with Accutase and plated at a density of 104 cells/cm2 in 6-well plates and cultured in fibroblast growth medium (Lonza) supplemented with 20 ng/ml FGF2 and 10 μM SB431542 ...
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bioRxiv - Developmental Biology 2021Quote: ... and E15.5 (n=2–6) Six2-TROMA-1 stained kidneys were embedded in 1 % low melting agar (50100; Lonza Group). The lateral cortex of the kidneys was imaged with a Nikon A1R MP+ multiphoton microscope (Tokyo ...
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bioRxiv - Cancer Biology 2020Quote: ... Cell line authenticity was confirmed by STR genotyping (July 2019) and mycoplasma testing was performed every 4-6 weeks (MycoAlert, Lonza).
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bioRxiv - Microbiology 2022Quote: ... for 24 h then interleukin-2 (50U/ml) for 4-6 days before nucleofection (as recommended by the manufacturer, Lonza) or infection by HIV-1.
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... Successful test compounds from the mf assay were diluted to 10 μM and added to the trans-wells in 6 ml endothelial basal media with supplements (EGM-2 MV; Lonza). Twelve replicates (n = 6 wells ...
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bioRxiv - Genomics 2021Quote: ... were nucleofected with 2 µg of each sgRNA/Cas9 plasmid and 3 pmol or 30 pmol (for E14-STNΔTsixP) repair oligo (Supplementary Table 6) using the Lonza 4D-Nucleofector with the P3 Primary Cell 4D-NucleofectorTM kit (Lonza) and CP-106 (for TXΔXertP and E14-STNΔTsixP ...
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bioRxiv - Cell Biology 2021Quote: ... were isolated from healthy donors (n=3) as described[4] and seeded on 6 cm dishes within BEGM media (Lonza) before transduction with lentivirus (empty vector (Origene ...
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bioRxiv - Immunology 2021Quote: Spleens and cervical lymph nodes were isolated from 6-10 weeks old C57B6 mice and homogenized through a sieve in B cell culture medium (RPMI-1640 (Lonza), 10% fetal calf serum (FCS ...
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bioRxiv - Immunology 2023Quote: Hepatocytes (7×105 cells) were transfected with various plasmids (at 6 μM or indicated concentrations) using the Mouse/ Rat hepatocyte Nucleofector kit (Lonza) following the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2023Quote: ... 5-6 million primary human keratinocytes were electroporated with 1 nmol siPools using the Amaxa human keratinocyte Nucleofector kit (Lonza) according to the manufacturer’s instructions and the T-018 program of the Amaxa Nucleofector II device (Lonza) ...
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bioRxiv - Bioengineering 2022Quote: ... 30 µl was mixed with 6 µl 6X gel loading dye and run on a 50 ml gel containing 2% SeaKem Agarose (Lonza), 1x Tris-Acetate-EDTA (Boston BioProducts) ...
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bioRxiv - Bioengineering 2023Quote: ... washed three times in PBS and cultured on collagen-coated 6-well plates in endothelial growth medium (EGM-2, Lonza) composed of endothelial basal medium supplemented with 2% fetal bovine serum ...
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bioRxiv - Immunology 2024Quote: ... were transfected with various plasmids (at 6 μM concentration) and siRNA (Dharmacon siGENOMESMARTpool) using the Mouse/ Rat hepatocyte Nucleofector kit (Lonza) following the manufacturer’s protocol ...
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bioRxiv - Immunology 2024Quote: ... 8×106 CTLs were transfected with 6 µg of plasmid DNA of each construct with P3 Primary Cell 4D-Nucleofector X Kit (Lonza). For the CLEM experiments the cells were transfected with TSP-1-GFPSpark and TSP-4-mCherry ...
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bioRxiv - Neuroscience 2024Quote: ... the EBs were transferred to 6-well plates (15 EBs/well) and cultured in X-VIVO15 medium (Lonza, Basel, Switzerland) supplemented with 1% GlutaMAX ...
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bioRxiv - Cell Biology 2024Quote: ... cells (5×105/well in 6-well plates) were transfected with 2 μg of DNA by nucleofection using Amaxa device (Lonza) following manufacturer’s instructions ...
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bioRxiv - Cell Biology 2020Quote: Bone marrow cells were extracted from long bones of C57BL/6J mice 6 to 8 weeks of age as described (Guimbal et al.. 2019) and cultured in α-minimal essential medium (αMEM. Lonza) containing 10% Fetal Bovine Serum (FBS ...
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bioRxiv - Neuroscience 2022Quote: ... After four days 12 EBs were transferred into each well of 6 well plate in X-VIVO 15 (Lonza, BE02-060Q) containing 100 ng/mL M-CSF (Peprotech ...
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bioRxiv - Immunology 2020Quote: ... Slices were immediately placed in a 6-well plate containing 3 mL per well of “complete RPMI”: RPMI (Lonza, 16-167F) supplemented with 10 % FBS (VWR ...
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bioRxiv - Immunology 2020Quote: ... and brachial lymph nodes were collected and then embedded in 6 % w/v low melting point agarose (Lonza, Walkersville MD, USA) in 1X PBS ...
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bioRxiv - Genomics 2021Quote: The MPRA libraries were electroporated in 4 to 6 million cells each using the Nucleofector 2b or 4D (Lonza, Basel, Switzerland) with 6 µg of plasmid DNA and program T-030 or Y-001 and DS-132 ...
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bioRxiv - Physiology 2024Quote: The gRNA plasmid (1µg) and donor template plasmid (3µg) were introduced into 3T3-L1 cells (1X 10 6 cell) by electroporation (LONZA, Basel, Switzerland). After 48h of electroporation ...
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bioRxiv - Physiology 2022Quote: ... 5 million cells were washed with phosphate buffered saline (PBS) and electroporated with 4-6 μg of appropriate plasmid construct using an Amaxa cell nucleofector (Lonza laboratories) and nucleofection reagent (362.88 mM ATP-disodium salt ...
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bioRxiv - Developmental Biology 2021Quote: Human isogenic ESCs containing different lengths of CAG repeats in the HTT Exon1 locus (HD-RUES2 6) were nucleofected using the Cell Line Nucelofector II (Kit L from Lonza, Walkersville, MD) by applying the B-016 program ...
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bioRxiv - Cell Biology 2020Quote: ... 1×106 cells (grown in 6-well dishes) were subjected to nucleofection with Amaxa® Cell Line Nucleofector® kit V (VCA-1003, Lonza), following the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2021Quote: ... Collected cells were counted at a density of 100,000 per well and seeded on matrigel pre-coated 6-well plates supplied with KBM-Gold keratinocyte growth medium (Lonza, Cat# 00192060) at 37°C in a humidified chamber with 5% carbon dioxide ...
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bioRxiv - Cancer Biology 2021Quote: ... Primary uveal melanoma cells were equally divided onto two wells of a fibronectin-covered 6-well tissue culture plate and grown in 5% CO2 in MDMF medium which consists of HAM’s F12 (Lonza, Walkersville MD, USA) supplemented with 1 mg/mL BSA (Sigma-Aldrich ...
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bioRxiv - Cell Biology 2023Quote: ... RPE1 cell pellets containing 1×10^6 cells were resuspended in Nucleofection Solution for Primary Mammalian Epithelial Cells (Lonza cat# VPI-1005) in the presence of nucleofection enhancer (cat# 1075915 ...
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bioRxiv - Microbiology 2022Quote: ... pre-coated cell culture 6-well plate with EBM-2 endothelial media supplemented with 10% FBS and a growth factor bullet kit (Lonza, Walkersville, USA) 3 mL/well ...
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bioRxiv - Neuroscience 2020Quote: ... cells were subjected to electroporation protocol O-003 in an Amaxa Biosystem (Lonza) and plated onto 12-mm poly-D-lysine- and laminin-coated glass coverslips ...
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bioRxiv - Neuroscience 2023Quote: ... The RNP and HDR donor was delivered to MIN-6 cells by electroporation using the Lonza 4D-Nucleofector (Lonza Group AG, Basel, Switzerland), using the 96-well SE reagent kit and program CM-150 ...
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bioRxiv - Neuroscience 2022Quote: ... Harvested EBs were transferred to a tissue-culture treated 6-well plate (Falcon, #353224) in 3 ml of EB Differentiation media: X-VIVO 15 (Lonza Biosciences, #04-418Q) + 1% Glutamax (Life Technologies ...
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bioRxiv - Bioengineering 2023Quote: Cells were cultured at 37°C and 5% CO2 in cell-specific media: primary human umbilical vascular endothelial cells (HUVECs, Lonza; up to passage 6) in EGM-2 (Lonza ...
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bioRxiv - Cell Biology 2020Quote: ... Oil Red O staining was performed as described by the manufacturer (Lonza AG, Basel, Switzerland) and was followed by haematoxylin and eosin (H&E ...
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bioRxiv - Neuroscience 2023Quote: ... were added to 20 µL P3 buffer containing 1.6 × 105 Accutase-dissociated iPSCs and the nucleofection procedure was carried out in a 16-well Amaxa 4D cuvette (Lonza; Primary Cell P3, pulse code CA137). Cells were cultured for 3 days under cold-shock conditions (32 °C/5% CO2 ...
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bioRxiv - Cell Biology 2024Quote: ... on a 6-well plate for 5 days and seeded at 5 x 104 cells in complete EGM2™ media (Lonza Bioscience, CC-3156 and CC-4176) on Basement Membrane Matrix (Fisher Scientific ...