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Citations for Lonza :
51 - 63 of 63 citations for 2x SYBR Green qPCR Master Mix since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2021Quote: ... mouse embryonic fibroblasts (MEFs) or African green monkey kidney fibroblasts (COS7) were cultured (37°C, 5% CO2) in DMEM (Lonza), supplemented with 10% fetal bovine serum (Life Technologies) ...
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bioRxiv - Microbiology 2023Quote: ... 190 μL was mixed with 10 μL of 20X SYBER Green I nucleic acid stain (Lonza Walkersville Inc, Walkersville, MD) in a well of a transparent 96-well plate and incubated in the dark for 15 minutes before being placed in the Guava easyCyte HT flow cytometer (488 nm ...
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bioRxiv - Physiology 2023Quote: ... 2 μl of KCNQ2/3 cDNA plasmid (0.9 g/l) and 1 μl of pmax green fluorescent protein vector (0.5 g/l) (Lonza, Cologne, Germany) were diluted in 125 μl of Opti-MEM medium (Life Technologies) ...
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bioRxiv - Molecular Biology 2019Quote: ... A total of 5 × 105 cells were suspended in 100 μL of Nucleofector V solution mix (Lonza, VCA-1003) with 2 μg of GZMA and RASGRP1 CRISPR/Cas9 plasmids and subjected to nucleofection using the cell line-specific program T-O17 ...
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bioRxiv - Neuroscience 2023Quote: ... TG trigeminal ganglion neurons were transfected with 3 µg of NaV1.7-PEPCy3 plasmid and 0.75 µg green fluorescent protein (GFP) using the rat neuron 4D-Nucleofector solution (P3 Primary Cell Solution, program DR 114; Lonza Biosciences). Neurons were plated on round bottom glass dishes (cat ...
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bioRxiv - Cell Biology 2022Quote: hTERT immortalized RPE-1 (RPE1) cell lines were grown in an 1:1 mix of DMEM and F-10 (Lonza) and Human Embryonic Kidney (HEK ...
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bioRxiv - Cell Biology 2020Quote: ... The cell / Amaxa solution mixture was added to the RNP mix and then pipetted into the bottom of a 96-well nucleofection plate (Lonza). This sample was then nucleofected using the 4D-Nucleofector Core unit and 96-well shuttle device (Lonza ...
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bioRxiv - Neuroscience 2023Quote: ... 01F49i-N-B7 iPSCs were dissociated to single cells and 250,000 cells transfected with 25 μL of the prepared transfection mix containing 20 µL of nucleofection buffer (P3 Primary Cell 4D-NucleofectorTM X Kit S, Lonza), 5 µL of the RNP complex ...
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bioRxiv - Genomics 2023Quote: ... 5 µg of the 4sgRNA plasmid or 10 µM synthetic guide RNAs were mixed and the cell/reagent/nucleofection mix was transferred to Nucleofection cuvette strips (Lonza). Cells were electroporated using a 4D nucleofector (4D-Nucleofector Core Unit ...
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bioRxiv - Cancer Biology 2022Quote: ... and mixed by pipetting with the full gRNA-Cas9 complex mix previously assembled then added in the electroporation chamber well (Lonza, V4XP3032). Cells were electroporated with the program EO-100 using the Lonza Nucleofector ...
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bioRxiv - Cancer Biology 2022Quote: ... Pelleted organoids were resuspended in 20uL of nucleofection mix and transferred to electroporation chamber (Lonza kit, #V4XP-3032, 96-well format) for electroporation using Lonza X Unit Nucleofector under the [ES ...
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bioRxiv - Physiology 2020Quote: Growth media used for the expansion of human muscle-derived cell populations consisted of Hams F-10 nutrient mix (Lonza, Basel, Switzerland) with added L-glutamine (2.5 mM) ...
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bioRxiv - Bioengineering 2023Quote: ... MCF-10A cells were cultured using an MEGM™ Mammary Epithelial Cell Growth Medium BulletKit™ without gentamycin-amphotericin B mix (Lonza) following ATCC’s instructions ...