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Citations for Lonza :
51 - 100 of 1130 citations for 1 Benzyl 3 Tert Butyldimethylsilyl Oxy Methyl Piperidin 4 One since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biophysics 2021Quote: ... HUVECs (Lonza, expanded to passage 3) were transduced with GFP tagged VE-cadherin (65 ...
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bioRxiv - Bioengineering 2020Quote: ... For transfection 4 × 106 cells/mL cells were resuspended in ProCHO-4 Medium (Lonza) supplemented with 4 mM Ultraglutamine (Lonza) ...
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bioRxiv - Cancer Biology 2021Quote: ... One million cells were electroporated using the Amaxa 4D Nucleofector kit (V4XC-9064, Lonza). Guide RNA and Cas9 complexes were pre-formed using 160μM crRNA annealed to 160μM tracrRNA (Dharmacon ...
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bioRxiv - Molecular Biology 2024Quote: ... 0.1% (v/v) NP40) followed by one last wash with molecular grade water (Lonza). The beads were then subjected to RNase digestion in a 25 ul reaction containing 10mM Tris-HCl (pH 7.5) ...
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bioRxiv - Cell Biology 2023Quote: ... or 4-D nucleofector (LONZA) according to the manufacturer’s instructions.
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bioRxiv - Genomics 2022Quote: ... donors (passage 4-9; Lonza) were grown in complete Endopan-3 medium kit (PAN-Biotech ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... Measured tissues originated from six F508del homozygous patients (CF patient #1: CF-AB0609, female, 31 years-old; CF patient# 3: #28388-0000450918, Lonza, male ...
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bioRxiv - Cell Biology 2024Quote: ... together with a plasmid encoding the piggyBac transposase in a 3:1 ratio (vector:transposase) using the P3 Primary cell 4D-Nucleofector™ kit (Lonza). Prior to targeting ...
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bioRxiv - Molecular Biology 2024Quote: ... The cells were first electroporated with a PiggyBac-configured plasmid containing the Dox-inducible NbALFA-ABEL degrader cassette and a plasmid encoding the PiggyBac transposase at a 3:1 mass ratio via nucleofection (solution E with program CM138) according to manufacturer’s instructions (Lonza Inc.), followed by puromycin selection (5μg/ml ...
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bioRxiv - Synthetic Biology 2024Quote: ... The optimised sequences were used to design gBlocks with appropriate overhangs (Fig. 1-3) and cloned into pMAX-GFP plasmid from Lonza, digested with KpnI and SacI (Fig ...
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bioRxiv - Bioengineering 2024Quote: SP8 or DP T cells were isolated as described above and stimulated in vitro using irradiated K562 CD19-CD137L aAPCs at a 1:3 aAPC:T cell ratio in X-VIVO™ 15 (Lonza), supplemented with 5% human AB serum (Cat ...
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bioRxiv - Bioengineering 2022Quote: ... and 3% FBS (all from Lonza Biosciences), termed astrocyte medium ...
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bioRxiv - Bioengineering 2021Quote: ... were used between passages 3-5 for paxillin experiments and culture medium was changed every 2-3 days (Lonza FBM Basal Medium supplemented with 2 v/v% fetal bovine serum (FBS) ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: Dry powder (2-3 mg) was loaded into an HPMC size 3 capsule (VCaps® Plus, Lonza, Morristown, NJ). The capsule was placed in a Plastiape high resistance RS00 DPI that was then attached to a Next Generation Impactor (NGI ...
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bioRxiv - Microbiology 2021Quote: ... Vero cell cultures were seeded at 1-3 × 104 cells/cm2 in Dulbecco’s minimal essential medium (DMEM, LONZA, Alpharetta, GA, USA) supplemented with 9% foetal bovine serum (FBS ...
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bioRxiv - Evolutionary Biology 2020Quote: ... and PX458-AAVS1 (3 µg) were electroporated into 1.3×106 H9 cells using the Human Stem Cell Nucleofector Kit 1 (Lonza, VPH-5012). Following electroporation the cells were seeded across 3-wells of a 6 well plate coated with Matrigel in StemFlex supplemented with RevitaCell supplement (Thermo Fisher ...
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bioRxiv - Bioengineering 2020Quote: ... supplemented with 4 mM Ultraglutamine (Lonza). 0.625 μg of plasmid DNAs per million cells followed by 2.5 μg polyethyleneimine per million cells (Polysciences ...
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bioRxiv - Immunology 2021Quote: ... suspension CHO cells expressing wildtype human CTLA-4 were plated at 1 × 106 cells/well in DPBS (Lonza, Basel, Switzerland) containing 0.5% BSA (MilliporeSigma ...
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bioRxiv - Microbiology 2020Quote: ... The same number of cells (2 x 106 cells) were lysed in 100 µl of 1% CHAPS/PBS and analyzed on 4-20% SDS-PAGE gel (Lonza). The envelope proteins were detected by 2F5 (specific for gp41 ...
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bioRxiv - Neuroscience 2024Quote: ... along with 20 μg recombinant Cas9 (IDT) and 1 pmol of each bridging oligonucleotide using the P3 Primary Cell 4-D-Nucleofector kit (Lonza) using the CA-137 program34 ...
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bioRxiv - Cell Biology 2024Quote: ... supplemented with 11 mM glucose and 25 mM HEPES (4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid) pH 7.4 (Lonza, Basel, Switzerland). The apical (AP ...
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bioRxiv - Bioengineering 2024Quote: ... The mixture was transferred to a confocal plate and subsequently UV crosslinked at 15 mW/cm2 for 1 minute and cultured for 4 days in EGM-2 media (Lonza).
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bioRxiv - Immunology 2022Quote: ... supplemented with fetal calf serum (FCS, 3%, Lonza) and HEPES (1.8%) ...
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bioRxiv - Immunology 2022Quote: ... supplemented with fetal calf serum (FCS, 3%, Lonza) and HEPES (1.8% ...
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bioRxiv - Microbiology 2023Quote: ... and 3 ml of ACK lysis buffer (Lonza) was added to the cells and incubated for 5 min ...
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bioRxiv - Molecular Biology 2024Quote: ... 3% MetaPhorTM Agarose gels (Cat. No. 50181, Lonza) were used ...
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bioRxiv - Neuroscience 2024Quote: ... were nucleofected into 3 million cells by LONZA 4D-Nucleofector using program CB-150 ...
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bioRxiv - Cancer Biology 2022Quote: ... NHEM-Ad cells were grown in MGM-4 Melanocyte Growth Medium-4 BulletKit (Lonza, Cat# CC-3249). We added penicillin (50 U/mL ...
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bioRxiv - Molecular Biology 2024Quote: ... Deletion of 4 nucleotides was then directly checked on 4% low melting MetaPhor® agarose gel (Lonza) and in some cases verified by sequencing.
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bioRxiv - Molecular Biology 2021Quote: ... and 199 (M199) medium (3: 1) supplemented with 10% fetal bovine serum (FBS),10 mM glutamine and penicillin-streptomycin (all from Lonza, Basel, Switzerland) and their purity was verified with the fibroblast marker TE-7 (Merck ...
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bioRxiv - Bioengineering 2024Quote: ... at a ratio of 3:1 bead/cell and 20 IU of IL-2 (Preprotech, 200-02) in X-Vivo media (Lonza, 02-053Q) supplemented with 5% human serum and Pen/Strep ...
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bioRxiv - Microbiology 2024Quote: ... One million cells resuspended in 100 μL of SF Cell Line Nucleofector solution (Lonza V4XC-2012) were electroporated with 2 μg plasmid using pulse code FF-120 (Lonza Amaxa 4D Nucleofector) ...
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bioRxiv - Immunology 2022Quote: ... and 4 mM of L-glutamine (Lonza). HLA-E expressing K562 cells were sorted and maintained in complete RPMI supplemented with 2 µg/mL blasticidin (Sigma-Aldrich ...
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bioRxiv - Biophysics 2021Quote: ... The WT and mutant genes were then amplified from these expression plasmids using the PCR primers 5’-GCGCTAGCAGCACCCATATGCCTGAG-3’and 5’-ACGAAGCTTTCAGTGGTGGTGGTGGT-3’and subcloned into the pMaxCloning vector (Lonza, VDC-1040) via NheI and BamHI sites to generate the pMax_WT_NEIL1 and pMax_Mutant_NEIL1 expression plasmids that were utilized throughout this analysis.
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bioRxiv - Genomics 2022Quote: ... One million sorted Pro-E cells were resuspended with 100 μl solution P3 for primary cells (Lonza); preassembled RNP were then added to the cell suspension for nucleofection ...
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bioRxiv - Molecular Biology 2023Quote: One million Aire wild-type mTEC 3.10 cells were suspended in 100 μL of nucleofector solution (Lonza) containing 20 pmol of spCas9-GFP ...
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bioRxiv - Microbiology 2024Quote: ... primary cells from six foreskin donors were used: five isolated in house and one commercially available (Lonza).
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bioRxiv - Neuroscience 2021Quote: ... embedded in 3% agarose (Lonza, # 50004, Rockland, ME, USA) and coronally sectioned (Leica VT1000S vibratome ...
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bioRxiv - Cell Biology 2023Quote: ... 4 μM Alt-R Cas9 Electroporation enhancer (IDT) and 4 μM single-strand DNA homology template (Ultramer DNA Oligonucleotide, Lonza). After electroporation ...
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bioRxiv - Biochemistry 2021Quote: Plateable, cryopreserved primary human hepatocytes (pHep) from one male donor (18 years, BMI 28.7) were purchased from Lonza, Switzerland ...
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bioRxiv - Microbiology 2021Quote: ... Each transfection reaction was transferred to one well in 16-well nucleofection strip (Lonza; Cat. No. V4XC-2032). The nucleofection strip was placed in the X-unit (Lonza ...
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bioRxiv - Cell Biology 2024Quote: ... expanded in submerged culture for one or two passages in bronchial epithelial growth medium (BEGM, Lonza, Walksville, MD), and then seeded on Transwell membranes (Corning ...
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bioRxiv - Neuroscience 2021Quote: ... a 4% agarose (SeaPlaqueTM GTGTM Agarose, 50111; Lonza) solution was prepared in 0.1M PBS and left at 45°C in a dry bath ...
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bioRxiv - Bioengineering 2022Quote: ... supplemented with 4 mmol/L L-glutamine (Lonza), 100 IU/ml Penicillin/100 mg/ml Streptomycin (Lonza) ...
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bioRxiv - Neuroscience 2021Quote: ... containing 4% low melting point agarose (50111, Lonza) which was then allowed to solidify at 4°C for a few minutes ...
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bioRxiv - Bioengineering 2020Quote: Passage 4 human mesenchymal stem cells (Lonza, Switzerland) were expanded to passage 5 in an incubator set at 37°C and 5% CO2 and fed with low glucose DMEM and glutamine ...
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bioRxiv - Cancer Biology 2020Quote: ... and embedded in 4% low-melting agarose (Lonza) preheated at 42 °C ...
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bioRxiv - Microbiology 2023Quote: ... 4 x 104 in 0.4 mL MEM (Lonza), 10% FBS (Corning) ...
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bioRxiv - Genomics 2022Quote: ... 4 million cells were electroporated (Amaxa Nucleofection, Lonza) with 25μg of L1-Cag/FLAG_NLS_ORF-1L plasmid and 15μg of pIC-Cre ...
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bioRxiv - Cell Biology 2023Quote: ... or Melanocyte Growth Basal Medium-4 (Lonza, Switzerland) after pigmented cells were dominant.