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Citations for Lonza :
901 - 950 of 5178 citations for Recombinant Human Programmed Cell Death 1 Ligand 2 His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2019Quote: ... All experiments were done with cells at passage 2 to 5 and cells were regularly checked for mycoplasma contamination (MicoAlert Lonza).
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bioRxiv - Molecular Biology 2022Quote: ... HEK293 cells were washed twice with PBS and 2 × 106 cells were resuspended in 100 μl of Solution V (Lonza) and then mixed with 5 μg purified sgRNA expression vector or the empty vector as a negative control before nucleofection using program Q-001 of a Nucleofector™ 2b (Lonza) ...
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bioRxiv - Cell Biology 2023Quote: ... Five million BJAB or OCI-LY1 cells were transfected with 2 μg plasmid DNA using the SF Cell Line 4D-Nucleofector X Kit L (Lonza) and the AMAXA Nucleofector biosystem (BJAB ...
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bioRxiv - Molecular Biology 2023Quote: ... spanning the whole SARS-CoV-2 genome was transfected into 2×106 HEK293T cells using the SF Cell Line 4D-Nucleofector-X Kit (Cat# V4XC-2012, Lonza) and the 4D-Nucleofector X Unit (Lonza ...
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bioRxiv - Cell Biology 2023Quote: ... JAWS transfection was achieved using 20 μg DNA and 2 x 106 million cells per 100 μl nucleofection solution from the Amaxa Nucleofector Mouse Dendritic Cell kit (Lonza), and the Y-001 program ...
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bioRxiv - Bioengineering 2024Quote: ... HSPCs (2×105 cells/condition) were transfected with RNP complexes using the P3 Primary Cell 4D-Nucleofector X Kit (Lonza) and the CA137 program (Nucleofector 4D ...
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bioRxiv - Cell Biology 2024Quote: ... Large scale D.Mel-2 cultures were prepared by diluting cells to a density of 106 cells/mL in Insect Xpress culture medium (Lonza) in Erlenmeyer flasks ...
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bioRxiv - Molecular Biology 2019Quote: ... ES cells were grown in 1:1 mixture of DMEM (Lonza BioWhittaker Cat ...
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bioRxiv - Microbiology 2022Quote: ... A 1:1 mixture of Bronchial Epithelial Cell Basal Medium (Lonza) with Dulbecco’s Modified Eagle Medium (DMEM ...
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bioRxiv - Immunology 2022Quote: ... were coated overnight at 4°C with 25μL of SEC fractions 1 to 5 diluted 1:2 with PBS (Cat. No. LZBE17-512F, Lonza). Wells were washed with 0.05% Tween-20 (Cat ...
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bioRxiv - Cell Biology 2022Quote: ... Cusps were placed in a petri dish filled with VEC medium (EGM™-2 MV Microvascular Endothelial Cell Growth Medium-2 BulletKit™ (Lonza, #C-3202)) and VECs were scraped into the medium using a sterile scalpel ...
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bioRxiv - Cell Biology 2022Quote: ... was induced in hVECs and pVECs on a 12-well plate using EGM™-2 MV Microvascular Endothelial Cell Growth Medium-2 BulletKit™ (Lonza, #CC-3202), without VEGF ...
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bioRxiv - Cell Biology 2020Quote: ... RNP complex was added to ssDNA GATA4 V267M repair template (IDT) and delivered to wildtype iPSCs by electroporation with the Amaxa human stem cell nucleofector starter kit (Lonza). Following electroporation ...
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bioRxiv - Genetics 2020Quote: ... ∼1×106 cells were transfected with pairs of 5 µg gRNA plasmid and 4 µg ssODN (Supplementary Table 3) using AmaxaTM Human CD34 Cell NucleofectorTM Kit (Lonza) in the 2B-NucleofectorTM on the U-08 setting ...
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bioRxiv - Genetics 2020Quote: ... Primary neonatal Human Umbilical Vein Endothelial Cells (HUVEC) were sourced from three suppliers to provide genetic diversity (Lonza, Gibco, PromoCell). HUVECs were expanded in endothelial cell growth medium (Sigma ...
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bioRxiv - Bioengineering 2019Quote: ... RFP-labelled human lung microvascular endothelial cells (VeraVec, Angiocrine Biosciences Inc., San Diego, CA, USA) were cultured in EGM2 medium (Lonza) supplemented with growth factors according to the manufacturer’s instructions (EGM2-MV BulletKit ...
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bioRxiv - Bioengineering 2020Quote: ... Human umbilical vein endothelial cell (HUVEC, C25271) and endothelial growth medium (EGM-2MV, CC4147) were purchased from LONZA (Basel, Switzerland). Rabbit anti-perilipin antibody (9349S ...
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bioRxiv - Pathology 2022Quote: The ability of CTEPH ECs to form tube-like structures on basement membrane matrix was compared to control human pulmonary artery endothelial cells (PAECs) obtained commercially (Lonza). The Cultrex® Reduced Growth Factor Basement Membrane Matrix (Trevigen cat.no ...
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bioRxiv - Microbiology 2021Quote: ... Cells were stimulated by CD3/CD28 Abs for 2 days and nuclofected with 100 μM RORC or non-targeting (NT1) siRNA (ON-TARGETplus SMART pool, Dharmacon) using the Amaxa Human T cell Nucleofector Kit (Amaxa, Lonza), according to the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2020Quote: Adipose-derived human mesenchymal stem cells (AD-hMSCs) and dermal fibroblasts (DF) used in this study were purchased from Lonza. AD-hMSCs and DF were isolated from a 42-year-old ...
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bioRxiv - Cell Biology 2019Quote: Mesenchymal stromal cells originating from the bone marrow of two healthy human donors were purchased from Lonza (Walkersville, MD, USA) and stored at −180°C in liquid nitrogen prior to experimentation ...
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bioRxiv - Microbiology 2021Quote: ... The human lung epithelial cell line Calu-3 (ATCC HTB-55) was maintained in DMEM F-12 (Lonza, Basel, Switzerland) supplemented with 10% FBS ...
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bioRxiv - Immunology 2020Quote: ... Activated cells were transfected using the Human T cell Nucleofector Kit (Amaxa Biosystem, #VPA-1002) and the Amaxa Nucleofector II system (Lonza), Program T-023 with ribonucleoprotein complexes ...
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bioRxiv - Immunology 2020Quote: ... Transfection of PBMCs: ASOs were electroporated into PBMCs using the Human T cell nucleofector kit (VPA-1002, Lonza, Basel, Switzerland). Transfection of TILs ...
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bioRxiv - Cell Biology 2023Quote: BCi-NS1.1 cells (passage 17-25) (kind gift of Dr. Ronald Crystal) and Normal Human Bronchial Epithelial cells (NHBE) (cat. no. CC-2541, Lonza, lot numbers 623950 ...
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bioRxiv - Cell Biology 2023Quote: ... HMLE cells were generated by introducing SV40 large T-antigen and human telomerase reverse transcriptase into a primary culture of normal mammary epithelial cells (Lonza). HMLE cells were cultured in MEGM ...
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bioRxiv - Microbiology 2023Quote: ... amazonensis promastigotes using the Human T-Cell Nucleofector kit and the Amaxa Nucleofector electroporator (program U-033, Lonza, Basel, Switzerland), for integration into the 18S rRNA locus within the nuclear DNA ...
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Efficient Methods for Target Gene Manipulation in Haematopoietic Stem Cell Derived Human NeutrophilsbioRxiv - Cell Biology 2023Quote: ... CD34+ HSPC selected as previously described were preconditioned in SFEM II and StemSpan cytokines for 72 hours and electroporated with the Cas9-gRNP mixture using the Human CD34+ Cell Nucleofector kit (Lonza) in a Lonza IIb Nucleofector using program U-008 ...
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bioRxiv - Immunology 2023Quote: ... of target genes in primary human T cells were generated by Cas9 ribonucleoprotein (RNP) electroporation using a 4D Nucleofector (Lonza) and P3 primary cell nucleofection reagents (Lonza ...
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bioRxiv - Bioengineering 2023Quote: ... Human umbilical vein endothelial cells (HUVEC) (cAP-0001, Angio Proteomie, USA) were suspended in EGM2 medium (CC-3162, Lonza, Switzerland) at a density of 1–2 × 107 cells/ml ...
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bioRxiv - Neuroscience 2024Quote: ... The RNP complex was subsequently transfected into dissociated iPSCs by electroporation with Lonza Nucleofector 2B using the Amaxa Human Stem Cell Nucleofector Starter Kit (Lonza) according to manufacturer’s manual ...
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bioRxiv - Cell Biology 2023Quote: ... and Cas9-GFP were then transfected into human iPSCs for INS mutation correction using embryonic stem cell Nucleofector Kit (VVPH-5012, Lonza). Transfected cells were cultivated in StemFlex medium (catalog #A3349401 ...
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bioRxiv - Cell Biology 2023Quote: ... and 2000 p mol of a degron-tagged control protein (mTagBFP2-RxxG) in a 100 µl nucleofection reaction (4D nucleofector kit SE plus supplement SF1, Lonza) 14 hours following nucleofection ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: Human dermal fibroblasts (HDFBs) (Lonza, Basel, Switzerland) cultured in low-glucose Dulbecco’s Modified Eagle Medium (DMEM ...
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bioRxiv - Bioengineering 2022Quote: Human skeletal myoblasts (hskMPs, Lonza, CC-2580) isolated from donated human tissue of 20 year old healthy Caucasians were used at passages 3-5 after obtaining permission for their use in research applications by the Cantonal Ethical Commission of canton de Vaud (CER-VD) ...
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bioRxiv - Bioengineering 2021Quote: ... Normal human lung fibroblasts (CC-2512, Lonza) were used between passages 3-5 for paxillin experiments and culture medium was changed every 2-3 days (Lonza FBM Basal Medium supplemented with 2 v/v% fetal bovine serum (FBS) ...
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bioRxiv - Immunology 2021Quote: ... and the Human Monocyte Nucleofector Kit (Lonza) as previously described (Schnoor et al. ...
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bioRxiv - Molecular Biology 2021Quote: Human aortic EC (HAEC; Lonza, CC-2535), human pulmonary artery EC (HPAEC ...
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bioRxiv - Neuroscience 2020Quote: ... Normal Human Astrocytes (NHA) (Lonza, Bäle, Switzerland) were cultured in astrocyte basal medium (ABM ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: Normal human lung fibroblasts (Lonza, Basel, Switzerland) were cultured in DMEM (high glucose ...
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bioRxiv - Cell Biology 2022Quote: Human Umbilical Arterial ECs (HUAEC) from LONZA® or PromoCell® were used between passage 1 to 3 ...
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bioRxiv - Microbiology 2022Quote: ... Primary human AECs were purchased from Lonza (Healthy = 2547 ...
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bioRxiv - Cell Biology 2019Quote: ... Human lung FBs were purchased from Lonza. Both FBs and PCs were serially cultured in Medium 199 (Gibco ...
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bioRxiv - Immunology 2020Quote: ... Human Monocyte Nucleofector buffer (V4XP-3024, Lonza) and the Lonza 4D-Nucleofector™ platform were used according to the manufacturers’ instructions with human monocytes nucleofection program ...
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bioRxiv - Microbiology 2020Quote: ... NHBE (primary human bronchial/tracheal epithelial, Lonza) cells were grown in Bronchial Epithelial Cell Growth Basal Medium (BEGM ...
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bioRxiv - Cell Biology 2020Quote: ... and human lung fibroblasts (LFs) (Lonza Inc.). We used optimized growth media supplied by Lonza Inc ...
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bioRxiv - Bioengineering 2020Quote: Primary adult human myoblasts (Lonza CC-2561), infantile Pompe fibroblast lines GM20089 and GM20090 (Coriell) ...
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bioRxiv - Molecular Biology 2022Quote: ... Primary human lung fibroblasts (CC-2512, Lonza) were grown and maintained in FGM-2 complete media ...
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bioRxiv - Bioengineering 2022Quote: Bone marrow-derived human MSCs (Lonza Inc.) were cultured using NutriStem XF (biological Industries ...
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bioRxiv - Cell Biology 2022Quote: Native human lung fibroblasts (Lonza, Basel, Switzerland) were grown in Gibco™ Minimal Essential Media Alpha (Thermofisher ...