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Citations for Lonza :
901 - 950 of 1183 citations for Human METTL3 shRNA Plasmid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genetics 2019Quote: ... Human multipotent adipose-derived stem cells (hMADS) were cultured in growth medium which consisted of: DMEM (Lonza, BE12-707F), 10% FBS ...
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bioRxiv - Immunology 2019Quote: ... washed once in PBS and resuspended in Mouse B cell Nucleofector Solution with Supplement (murine B cells) or Primary Cell Nucleofector Solution 3 with Supplement (human B cells) prepared to the manufacturer’s instructions (Lonza) at a concentration of 4 - 5 × 106 cells / 86 μL ...
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bioRxiv - Microbiology 2020Quote: A549 cells (human lung carcinoma cell line, ATCC CCL-185) were grown in Dulbecco’s modified eagle medium (DMEM) (Lonza) supplemented with 10% heat-inactivated fetal bovine serum (FBS ...
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bioRxiv - Genetics 2020Quote: ... 20 µg ssODNs and 4 µg pSpCas9(BB)-2A-GFP construct using Human Stem Cell Nucleofector Kit 2 (Lonza). For the generation of UE-RASGEF1A-int1-KO and PIK3C2B-int10-KO hPSC lines ...
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bioRxiv - Cell Biology 2020Quote: ... Eriksson’s lab) and human osteosarcoma (U2OS) (ATCC HTB-96) cells were cultured in high-glucose DMEM (Lonza, BE12-119F), supplemented with 10% FBS (Gibco ...
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bioRxiv - Genetics 2019Quote: PBT cells were transfected with 5 μg DNA for 5.106 cells in 100 μl of Human T Cell Nucleofector Solution (Lonza) with the program U-014 (Amaxa Biosystems).
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bioRxiv - Microbiology 2021Quote: Human airway tracheobronchial epithelial cells isolated from airway specimens from donors without underlying lung disease were provided by Lonza, Inc ...
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bioRxiv - Microbiology 2021Quote: Primary human bronchial epithelial cells (HBECs) derived from a non-smoking donor (Cat# CC-2540, male; Lonza; Donor 1) or derived directly from a patient at Cambridge University Hospitals NHS Trust (Research Ethics Committee Reference 19/SW/0152 ...
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bioRxiv - Microbiology 2021Quote: Human airway tracheobronchial epithelial cells isolated from airway specimens from donors without underlying lung disease were provided by Lonza, Inc ...
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bioRxiv - Cell Biology 2022Quote: Human umbilical vein endothelial cells (HUVEC) were cultured between passage 3 and 6 in EGM-2 complete medium (Lonza)3,52–55 ...
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bioRxiv - Neuroscience 2022Quote: Primary human astrocytes were cultured on Matrigel-coated (0.08 mg/well) 6-well plates in astrocyte growth medium (Lonza). On the day of plating ...
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bioRxiv - Cancer Biology 2022Quote: ... HEK293T cells (human embryonic kidney; LGC Nordic ATCC CRL-3216) were cultured in DMEM high glucose (#BE12-614Q, Lonza), supplemented with 2 mM L-glutamine ...
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bioRxiv - Bioengineering 2023Quote: ... 0.1% r-human fibroblast growth factor (rhFGF) and 0.1% Gentamicin sulfate amphotericin-B (GA-1000) (Lonza, Quakertown, PA, USA). HLFs were used from passages 1 to 10 ...
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bioRxiv - Neuroscience 2023Quote: Electroporation with freshly assembled RNP complex was performed on hiPSCs using the Human Stem Cell Nucleofector Kit 1 (Lonza) in conjunction with the Amaxa Nucleofector 2b system (Lonza ...
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bioRxiv - Cancer Biology 2022Quote: ... The B16 and the human melanoma cells were cultured in Dulbecco’s modified Eagle’s medium (DMEM, Lonza; BE12-604F/U1) supplemented with 10% fetal bovine serum (FBS ...
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bioRxiv - Cell Biology 2022Quote: Normal human bronchial epithelial cells (HBECs) from 3 independent donors (Supplemental Table S1) were obtained from Lonza (CC-2540) and cultured in BEGM growth media (Lonza CC-3170) ...
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bioRxiv - Immunology 2023Quote: ... Cas9-gRNA ribonucleoproteins were assembled as described previously53 and nucleofected into 5×106 monocytes in 100μL nucleofection buffer (Human Monocyte Nucleofection Kit, Lonza) using a Nucleofector 2b (Lonza ...
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bioRxiv - Immunology 2023Quote: Low passage primary human bronchial epithelial cells (HBEC) from healthy adult donors were obtained commercially (Lonza, Walkersville, MD, USA) and cultured at air-liquid interface according to the manufacturer’s instructions (Stem Cell Technologies ...
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bioRxiv - Microbiology 2023Quote: Human airway tracheobronchial epithelial cells isolated from airway specimens from donors without underlying lung disease were provided by Lonza, Inc ...
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bioRxiv - Immunology 2023Quote: ... The precomplexed HDR-RNP complex was electroporated into primary human T cells as described above using P3 buffer (Lonza). The T cells were cultured in T cell media with 300 IU/ml IL-2 for 7-10 days ...
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bioRxiv - Bioengineering 2023Quote: Primary human alveolar epithelial cells (HPAECs, CellBiologics) were cultured at 37°C and 5% CO2 with SABM medium (Lonza), SAGM supplements (Lonza) ...
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bioRxiv - Biochemistry 2024Quote: Human HeLa cells (ATCC) were grown at 37°C in 5% CO2 using Dulbecco’s Modified Eagle Medium (Lonza, USA) supplemented with 10% FBS superior (Biochrom ...
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bioRxiv - Bioengineering 2024Quote: Commercially available primary adult human mesenchymal stem cells (hMSCs) from Donor 47506 were used (Lonza Group Ltd., Basel, Switzerland). Cells were cultured at passage 3 and seeded in triplicate in hMSC Growth Medium (MSCGM™ Mesenchymal Stem Cell Growth Medium ...
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bioRxiv - Cancer Biology 2024Quote: ... Human Umbilical Vein Endothelial Cells (HUVECs) were cultured in complete EGM-2 (cat# CC-3162, Lonza, Allendale, NJ, USA) and were not used beyond passage five for any experiments.
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bioRxiv - Cancer Biology 2024Quote: ... and (iv) the NDF-2 and NDF-3 cell lines (human neonatal dermal fibroblast cell lines, #CC-2509; Lonza Bioscience ...
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bioRxiv - Cell Biology 2019Quote: ... Recombinant plasmids were verified by Sanger sequencing and electroporated into E14 cells using the Nucleofector II platform (Lonza) (program A-013) ...
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bioRxiv - Immunology 2022Quote: ... 2×106 B cells were then nucleofected with 2 μg of plasmid DNA using Nucleofector Kit V (Lonza) and rested for at least 16-24 hours using complete media containing 5 ng/ml BAFF and lacking LPS ...
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bioRxiv - Bioengineering 2022Quote: ... Transfection efficiency was assessed by including a control plasmid encoding for constitutive GFP (pmax-GFP, Lonza, #V4YP-1A24). Media was changed within 7-8 hours after transfection to E8 flex and ...
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bioRxiv - Immunology 2019Quote: ... gRNA and Cas9 containing plasmids were introduced to prostate epithelial cells using the basic nucleofector kit (Amaxa, Lonza) following the manufacturer’s instructions for primary mammalian epithelial cells (program W001) ...
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bioRxiv - Evolutionary Biology 2020Quote: ... 5 µl OptiMEM were mixed with 0.75 µl Lipofectamine and then added to 25 µl OptiMEM containing 0.1 µg GFP pMAX plasmid (Lonza) and 1 µl P3000 reagent ...
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bioRxiv - Cell Biology 2021Quote: ... at 0.1-1 μg/mL or as a control a GFP plasmid (PmaxGFP, Lonza, Cat. No. D-00059) using Lipofectamine 3000 (Invitrogen ...
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bioRxiv - Genomics 2022Quote: ... Wild type K562 cells were transfected with both the donor and guide plasmids by nucleofection (Lonza, program T016). Cells were selected 3 days after transfection with puromycin (2µg/mL ...
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bioRxiv - Pathology 2020Quote: ... Plasmid delivery through electroporation of passage three neurospheres was performed using Mouse neural stem cell nucleofector kit (Lonza).
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bioRxiv - Cancer Biology 2020Quote: Two million cells were harvested and transfected with or without 1.5μg of BRAFV600E plasmid (obtained from Dr. Dan Gough) using AMAXA Nucleofector (Lonza), according to supplier’s instructions ...
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bioRxiv - Cell Biology 2020Quote: Plasmids were electroporated in ESC using the Lonza NucleofectorTM 2B Device using program A-23 (Lonza, AAB-1001). 10 µg of plasmids were mixed with 5 millions ESC in buffer “Mouse ES cell nucleofector kit (Lonza ...
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bioRxiv - Genomics 2021Quote: ... K562 cells were grown to 1×106 and transfected with 1,000ng of gRNA plasmid and 3,000 ng of pCMV_AncBE4max_P2A_GFP (Amaxa® Cell Line Nucleofector® Kit V, Lonza). K562 cells were cultured for an additional 48 hours then single clones of green cells were isolated using flow cytometry (Aria II) ...
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bioRxiv - Cell Biology 2022Quote: ... expression constructs were transfected into cells using the Amaxa™ 4D-Nucleofector (10μg plasmid cDNA; Lonza, Basel, Switzerland) or JetPrime (2μg plasmid cDNA ...
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bioRxiv - Immunology 2023Quote: ... and nucleofected with 5 μg of a plasmid using program X-001 in the Nucleofector IIb device (Lonza) according to the manufacturer’s procedure ...
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bioRxiv - Cell Biology 2023Quote: For immunoprecipitations performed on THP-1 cells were first transfected with 2 μg of empty vector pCDNA 3.1 or plasmid containing 3x FLAG_Vamp-3 using the Amaxa 4D nucleoporator system (Lonza) with the Amaxa SG Cell line kit and program FF-100 and following manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2023Quote: ... Around five million WT serum-grown ESCs were transfected with 1μg of plasmids using Amaxa 4d Nucleofector (Lonza) and plated at a low density ...
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bioRxiv - Cell Biology 2020Quote: ... RNP complex was added to ssDNA GATA4 V267M repair template (IDT) and delivered to wildtype iPSCs by electroporation with the Amaxa human stem cell nucleofector starter kit (Lonza). Following electroporation ...
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bioRxiv - Genomics 2020Quote: ... Transfection of these iPSCs with the plasmid and Super piggyBacTM transposase mRNA (Transposagen) was done using the Human Stem Cell Nucleofector Kit 1 (VAPH-5012) by Nucleofector 2b Device (AAB-1001, Lonza) according to the manual ...
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bioRxiv - Immunology 2021Quote: ... suspension CHO cells expressing wildtype human CTLA-4 were plated at 1 × 106 cells/well in DPBS (Lonza, Basel, Switzerland) containing 0.5% BSA (MilliporeSigma ...
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bioRxiv - Physiology 2019Quote: ... 10% AIM-V and 100 units/ml penicillin/ streptomycin and transiently transfected using Nucleofector™ Kits for Human Melanocytes (Lonza) or magnetofection with PolyMag Neo magnetic beads (OZ Biosciences) ...
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bioRxiv - Genetics 2019Quote: ... Electroporation was performed using the Human Adult Dermal Fibroblast Nucleofector™ Kit and the Nucleofector™ II/2b device (Lonza). Both cell lines were harvested 72-hours post-transfection for qRT-PCR ...
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bioRxiv - Genetics 2020Quote: ... Primary neonatal Human Umbilical Vein Endothelial Cells (HUVEC) were sourced from three suppliers to provide genetic diversity (Lonza, Gibco, PromoCell). HUVECs were expanded in endothelial cell growth medium (Sigma ...
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bioRxiv - Cell Biology 2019Quote: Human lung microvascular endothelial cells (HLMVECs) and Endothelial growth media-2 (EGM-2) were purchased from Lonza (Walkersville, MD, USA). Lung endothelial cells (ECs ...
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bioRxiv - Bioengineering 2019Quote: ... RFP-labelled human lung microvascular endothelial cells (VeraVec, Angiocrine Biosciences Inc., San Diego, CA, USA) were cultured in EGM2 medium (Lonza) supplemented with growth factors according to the manufacturer’s instructions (EGM2-MV BulletKit ...
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bioRxiv - Bioengineering 2020Quote: ... Human umbilical vein endothelial cell (HUVEC, C25271) and endothelial growth medium (EGM-2MV, CC4147) were purchased from LONZA (Basel, Switzerland). Rabbit anti-perilipin antibody (9349S ...
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bioRxiv - Microbiology 2021Quote: ... Specific cells used were human bronchial epithelial cells (HBECs) derived from a non-smoking donor (Lonza; Cat# CC-2540, male); human bronchial epithelial cells from a male smoking donor undergoing bronchoscopy for a non-cancer indication ...