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Citations for Lonza :
901 - 934 of 934 citations for CD122 IL 2RB Human HEK293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2020Quote: Cryopreserved PHHs were purchased from vendors permitted to sell products derived from human organs procured in the United States of America by federally designated Organ Procurement Organizations (BioIVT, Baltimore, MD, and Lonza, Walkersville, MD). PHH lots included HUM4055A (54-year-old female Caucasian ...
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bioRxiv - Neuroscience 2020Quote: ... hESCs human cortical neurons were nucleofected with shRNAs constructs against ASH1L using the X-unit of the Amaxa nucleofector unit (Lonza, catalog # AAF1002X). Human neurons between days 30 to 32 of neuronal induction were single cell dissociated using single cell dissociation reagent (stem cell technologies CT catalog # 07174 ...
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bioRxiv - Cell Biology 2021Quote: ... OC activity was measured by releasing the europium conjugated human Type I collagen-coated on the bottom of the OsteoLyse™ Assay Kit (Lonza Biosciences) at each time point ...
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bioRxiv - Cancer Biology 2021Quote: ... Immortalized human bronchial epithelial BEAS-2B cells were purchased from ATCC and cultured in BEGM bronchial epithelial cell growth medium (Lonza #CC-4175). All the cells were grown at 37°C with 5% CO2.
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bioRxiv - Developmental Biology 2022Quote: ... Cells were electroporated with 10 µg lineage recorder DNA and 1 µg Sleeping Beauty transposase in 100 µl Human Stem Cell nucleofection buffer (Lonza, VVPH-5022) using the B-016 program of the 4D-Nucleofector (Lonza ...
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bioRxiv - Physiology 2020Quote: Growth media used for the expansion of human muscle-derived cell populations consisted of Hams F-10 nutrient mix (Lonza, Basel, Switzerland) with added L-glutamine (2.5 mM) ...
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bioRxiv - Immunology 2021Quote: Primary BEAS-2B (ATCC® CRL-9609TM) (normal human airway epithelial cells) as well as all the basal media and growth supplements were obtained from Lonza (Walkersville, MD). Cells were cultivated according to the instructions of the manufacturer on plastic dishes or flasks (BD Bioscience ...
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bioRxiv - Cancer Biology 2020Quote: ... 10 mmol/L HEPES (PAA Laboratory) and 1 ng/mL human basic fibroblast growth factor (bFGF) (Sigma-Aldrich) (Lonza, Walkersville, MD, USA). The generation number of ECs is maintained between 30-40 generations ...
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bioRxiv - Immunology 2019Quote: Primary human normal airway tracheobronchial epithelial cells from de-identified donors (NHBE) (sex: male and female) were provided by Lonza (Walkersville, MD) and grown in BEM media supplemented with the BEGM bullet kit (Lonza) ...
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bioRxiv - Evolutionary Biology 2020Quote: ... plasmids pSpCas9n(BB)-ZEB2-Guide-A &-B (0.5 µg of each plasmid) were electroporated into 1×106 H9 cells using the Human Stem Cell Nucleofector Kit 1 (Lonza, VPH-5012). Following electroporation ...
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bioRxiv - Cell Biology 2021Quote: ... 5 µg of sgRNA and 5 µg of ssDNA donor template (Table S1) using Human Stem Cell NucleofectorTM kit 1 (Lonza, VVPH-5012) according to the manufactures protocol and cells replated ...
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bioRxiv - Cancer Biology 2021Quote: ... Cells were transfected with pcDNA3.1 empty vector or pcDNA3.1 containing the human coding sequence of the VGLL2-NCOA2 fusion using the AMAXA cell line Nucleofection kit V (Lonza #VCA-1003;) and were selected in growth media containing 1μg/ml concentration of G418 (ThermoFisher #10131035) ...
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bioRxiv - Immunology 2020Quote: ... 1 × 106 BLaER1 cells were transfected with 2 μg plasmid DNA using the Human B Cell Nucleofector Kit and Nucleofector I device (program U-15; both Lonza, Basel, Schweiz). On day 2 post transfection ...
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bioRxiv - Molecular Biology 2023Quote: Induced pluripotent stem cells were transfected with a total of 5 μg target Cas9/gRNA plasmids with or without 1 μg repair templates using Human Stem Cell Nucleofector Kit (LONZA, Basel, Switzerland) and Nucleofector 2b device (LONZA ...
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bioRxiv - Cell Biology 2023Quote: ... The linearized construct and the purified schizonts were mixed with Nucleofector solution from an Amaxa human T cell Nucleofector Kit and electroporated using the Amaxa Nucleofector II device (Lonza, Köln, Germany). Directly after transfection 50 µl RPMI-1640 complete was added to the transfection reaction followed by intravenous injection into one SWISS mouse ...
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bioRxiv - Immunology 2023Quote: ... 10% FBS heat-inactivated and 1% Penicillin/Streptomycin. Human Small Airway Epithelial Cells (SAECs, Cat. CC-2547) and the corresponding medium were commercially obtained by Lonza (NJ, USA). Human Pulmonary Microvascular Endothelial Cells (HPMECs ...
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bioRxiv - Cell Biology 2023Quote: ... MCF10A cells (female human mammary epithelial cells) were purchased from ATCC (CRL-10317) and cultured as recommended by ATCC in MEGM (Lonza CC-3150) supplemented with bovine pituitary extract ...
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bioRxiv - Microbiology 2024Quote: ... 5 million parasites were electroporated with 5-10ug of digested plasmid with an AMAXA Nucleofector II using X-001 in Human T-cell Nucleofector Solution (Lonza VPA-1002).
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bioRxiv - Microbiology 2023Quote: ... BEAS-2B cells (human bronchial epithelial; CRL-9609ATCC) cells were grown in bronchial epithelial cell growth basal media (BEBM; Lonza/CC-3170). LLC-MK-2 cells (rhesus monkey kidney ...
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bioRxiv - Neuroscience 2020Quote: ... two pairs of sgRNA vectors and Cas9 nickase plasmid were transduced into the AD hPSCs by using Human Stem Cell Nucleofector® Kit 1 (Lonza VPH-5012). Three days after electroporation ...
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bioRxiv - Neuroscience 2021Quote: ... 1 × 106 cells were resuspended in 100 μl of the nucleofection hESC solution 1 (Human Stem Cell Nucleofector® Kit 1/ Lonza #VPH-5012) where 5 μg of plasmids were added previously ...
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bioRxiv - Immunology 2020Quote: ... Human buffy coats were initially added to 40 ml chemical-defined serum-free culture X-VIVO 15™ mediums (Lonza, Walkersville, MD, USA) and mixed thoroughly with 10ml pipette ...
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bioRxiv - Immunology 2022Quote: ... Activated cells were nuclofected with 100 µM AhR or non-targeting (NT1) siRNA (ON-TARGETplus SMART pool, Dharmacon) using the Amaxa Human T cell Nucleofector Kit (Lonza, Walkersville, MD, USA), according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2023Quote: ... plates were incubated at 37°C for 30 minutes in blocking buffer (PBS with 1% BSA, 5% FBS, and 1% human AB serum (Lonza, Walkersville, MD, USA), washed three times in PBS with 0.05% tween 20 and incubated with a commercial anti-SARS-CoV-2 Spike antibody (1/1000 ...
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bioRxiv - Cell Biology 2024Quote: The commercially available human myoblast (hMB) stock isolated from a healthy 35-year-old female (CC-2580, lot 0000483427; Lonza, Walkersville, MD, USA) was used [7,10] ...
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bioRxiv - Neuroscience 2020Quote: ... 6 µg of total plasmid DNA (2 µg for each of the three episomal vectors) were electroporated into 5×10^5 fibroblasts with the Amaxaμ Human Dermal Fibroblast Nucleofectorμ Kit (Lonza, Basel, Switzerland; VDP-1001). The fibroblasts were further cultured for 6 days to allow them to recover ...
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bioRxiv - Microbiology 2020Quote: ... The previously described human proximal tubular epithelial cell line HPT-1b[26] was maintained in renal epithelial cell growth media (Lonza, cat. no. CC-4127). To generate the HPT-1b-mCherry cell line ...
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bioRxiv - Cancer Biology 2019Quote: ... and 3-4 hours later were transfected with 2 μg of pBabe KRAS WT and pBabe KRAS G12D or pBabe KRAS Q61H plasmid DNA by electroporation using the Amaxa® Human T Cell Nucleofector® Kit (Lonza, Basel, Switzerland) following the manufacturer’s protocol ...
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bioRxiv - Bioengineering 2023Quote: Cells were cultured at 37°C and 5% CO2 in cell-specific media: primary human umbilical vascular endothelial cells (HUVECs, Lonza; up to passage 6) in EGM-2 (Lonza ...
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bioRxiv - Neuroscience 2023Quote: ... 4−106 cells were pelleted at 300xg and then resuspended in 100 μL prewarmed nucleofector solution (Human Stem Cell Kit 1, Lonza, catalog no. VPH-5012); 4 μg of NGN2 plasmid and 1.5 μg each of 1R and 1L plasmids were added directly to the resuspended cells ...
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bioRxiv - Bioengineering 2023Quote: ... Complete growth medium for culturing of human adipose derived stem cells (ASCs, ATCC SCRC-4000) consists of Lonza ADSC Basal Medium (Lonza, Lexington, MA PT-3273), 10% MSC FBS (ThermoFisher Scientific) ...
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bioRxiv - Immunology 2021Quote: Primary normal human bronchial epithelial cells (NHBECs) were obtained from a 65 year old Caucasian male without identifiers sourced from Lonza (NHBE CC-2540; Walkersville, MD). The NHBECs were revived from cryopreservation and expanded with PneumaCult™ Ex-Plus media (StemCell #05040 ...
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bioRxiv - Cell Biology 2024Quote: Human primary lung microvascular endothelial cells (LMVECs) and pulmonary artery endothelial cells (PAECs) from different donors were purchased from Lonza (Walkersville, MD. See Table S1). Cells were seeded onto plates and cultured in complete medium comprising of endothelial basal medium-2 (EBM-2 ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: Human umbilical vein endothelial cells (HUVEC) cells were grown at 37° C in the Endothelial cell basal medium-2 (LONZA #Cat No-CC-3156 and CC-4176) under the atmosphere containing 5% CO2 ...