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Citations for Lonza :
851 - 900 of 973 citations for Recombinant Human ABHD15 His tagged since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2022Quote: Primary human astrocytes were cultured on Matrigel-coated (0.08 mg/well) 6-well plates in astrocyte growth medium (Lonza). On the day of plating ...
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bioRxiv - Cancer Biology 2022Quote: ... HEK293T cells (human embryonic kidney; LGC Nordic ATCC CRL-3216) were cultured in DMEM high glucose (#BE12-614Q, Lonza), supplemented with 2 mM L-glutamine ...
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bioRxiv - Cancer Biology 2022Quote: ... The B16 and the human melanoma cells were cultured in Dulbecco’s modified Eagle’s medium (DMEM, Lonza; BE12-604F/U1) supplemented with 10% fetal bovine serum (FBS ...
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bioRxiv - Cell Biology 2022Quote: Normal human bronchial epithelial cells (HBECs) from 3 independent donors (Supplemental Table S1) were obtained from Lonza (CC-2540) and cultured in BEGM growth media (Lonza CC-3170) ...
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bioRxiv - Immunology 2023Quote: ... Cas9-gRNA ribonucleoproteins were assembled as described previously53 and nucleofected into 5×106 monocytes in 100μL nucleofection buffer (Human Monocyte Nucleofection Kit, Lonza) using a Nucleofector 2b (Lonza ...
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bioRxiv - Immunology 2023Quote: Low passage primary human bronchial epithelial cells (HBEC) from healthy adult donors were obtained commercially (Lonza, Walkersville, MD, USA) and cultured at air-liquid interface according to the manufacturer’s instructions (Stem Cell Technologies ...
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bioRxiv - Developmental Biology 2024Quote: ... The constructs were stably introduced into the human iPSCs and mouse EpiSCs by electroporation with a 4D Nucleofector (Lonza) or using lipofectamine (Invitrogen) ...
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bioRxiv - Molecular Biology 2024Quote: ... Plasmids were transfected into human U2OS cells (ATCC HTB-96) by Amaxa electroporation with Nucleofector™ Kit V (Lonza) and plated in 6 well plates containing glass slides.
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bioRxiv - Developmental Biology 2024Quote: ... The constructs were stably introduced into the human iPSCs and mouse EpiSCs by electroporation with a 4D Nucleofector (Lonza) or using lipofectamine (Invitrogen).
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bioRxiv - Microbiology 2024Quote: ... from Lonza and maintained at low passages as previously described.9 Normal human astrocytes (NHA) were commercially obtained (CC-2565, Lonza) and maintained using Astrocyte Growth Medium (AGM ...
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bioRxiv - Immunology 2024Quote: ... 107 schizonts were resuspended 100ul in human T cell transfection buffer containing 5μg digested DNA for transfection by U-33 program (Lonza).
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bioRxiv - Molecular Biology 2024Quote: Human fibroblasts were seeded in 12-well plates in adipogenesis differentiation medium consisting of ɑMEM (Lonza, Geleen, The Netherlands) supplemented with 10% FBS ...
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bioRxiv - Cancer Biology 2024Quote: ... Primary human umbilical vein endothelial cells (HUVECs) were maintained in an EGM-2 Bullet Kit (CC-3162, Lonza, USA). AS1842856 (Cat#HY-100596 ...
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bioRxiv - Biophysics 2024Quote: ... Human mesenchymal stem cells (hMSCs, ATCC) were cultured in DMEM with 10% FBS and differentiated with osteogenic medium (Lonza) on day 2 post-seeding ...
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bioRxiv - Bioengineering 2024Quote: ... Primary human T cells were cultured in complete X-VIVO 15 (cXVIVO 15) (Lonza Bioscience, Visp, Switzerland #04-418Q) which consists of 5% FCS (R&D systems ...
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bioRxiv - Cell Biology 2024Quote: Primary human mammary epithelial cells (HMECs) were cultured at 37°C with 5% CO2 in MEBM basal medium (Lonza) supplemented with MEGM SingleQuots (Lonza ...
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bioRxiv - Cell Biology 2020Quote: ... RNP complex was added to ssDNA GATA4 V267M repair template (IDT) and delivered to wildtype iPSCs by electroporation with the Amaxa human stem cell nucleofector starter kit (Lonza). Following electroporation ...
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bioRxiv - Genomics 2020Quote: ... Transfection of these iPSCs with the plasmid and Super piggyBacTM transposase mRNA (Transposagen) was done using the Human Stem Cell Nucleofector Kit 1 (VAPH-5012) by Nucleofector 2b Device (AAB-1001, Lonza) according to the manual ...
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bioRxiv - Immunology 2021Quote: ... suspension CHO cells expressing wildtype human CTLA-4 were plated at 1 × 106 cells/well in DPBS (Lonza, Basel, Switzerland) containing 0.5% BSA (MilliporeSigma ...
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bioRxiv - Genetics 2020Quote: ... ∼1×106 cells were transfected with pairs of 5 µg gRNA plasmid and 4 µg ssODN (Supplementary Table 3) using AmaxaTM Human CD34 Cell NucleofectorTM Kit (Lonza) in the 2B-NucleofectorTM on the U-08 setting ...
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bioRxiv - Genetics 2020Quote: ... Primary neonatal Human Umbilical Vein Endothelial Cells (HUVEC) were sourced from three suppliers to provide genetic diversity (Lonza, Gibco, PromoCell). HUVECs were expanded in endothelial cell growth medium (Sigma ...
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bioRxiv - Bioengineering 2020Quote: ... Human umbilical vein endothelial cell (HUVEC, C25271) and endothelial growth medium (EGM-2MV, CC4147) were purchased from LONZA (Basel, Switzerland). Rabbit anti-perilipin antibody (9349S ...
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bioRxiv - Cell Biology 2021Quote: ... Patients-derived iPS cells were electroporated with plasmids and single stranded DNA donor oligos using Human Stem Cell NucleofectorTM Kit 1 (Lonza). After 36 hours ...
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bioRxiv - Microbiology 2021Quote: ... Specific cells used were human bronchial epithelial cells (HBECs) derived from a non-smoking donor (Lonza; Cat# CC-2540, male); human bronchial epithelial cells from a male smoking donor undergoing bronchoscopy for a non-cancer indication ...
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bioRxiv - Cancer Biology 2020Quote: ... Immortalised human cerebral microvascular endothelial cells (hCMEC/D3) and Human Umbilical Vein Endothelial Cell (HUVEC) were maintained in Endothelial Basal Medium-2 (EBM-2, Lonza), containing 5% foetalbovine serum (FBS Serum Gold ...
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bioRxiv - Pathology 2022Quote: The ability of CTEPH ECs to form tube-like structures on basement membrane matrix was compared to control human pulmonary artery endothelial cells (PAECs) obtained commercially (Lonza). The Cultrex® Reduced Growth Factor Basement Membrane Matrix (Trevigen cat.no ...
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bioRxiv - Molecular Biology 2022Quote: ... and human ACTB (assay control, primer composition not disclosed by manufacturer) were loaded on a 2.5% agarose gel (Lonza, 50010) and stained with GelRed® (Merck ...
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bioRxiv - Bioengineering 2022Quote: ... Human hepatic stellate cell LX-2 was cultured in HEPES-buffered Dulbecco’s modified Eagle medium (DMEM) (Lonza BioWhittaker, Verviers, Belgium) supplemented with 4 mmol/L L-glutamine (Lonza) ...
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bioRxiv - Cell Biology 2022Quote: ... and diseased COPD human lung fibroblasts (DHLFs) from 3 donors (donor IDs: OF3353, OF3418 and OF3238) were purchased from Lonza and tested for their response to FGF2 and TGFβ stimulation.
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bioRxiv - Cancer Biology 2022Quote: ... siControl and siIRAK4 were purchased from Horizon Discovery and transfected into human AML samples using Amaxa Nucleofector Kit T (Lonza) (program number G-016 ...
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bioRxiv - Immunology 2020Quote: ... Rinsed cells were resuspended in buffer P4 (mouse) or P2 (human) at 1-10 x 106 cells/20 μL (Lonza). Non-targeting Alt-R crRNA #1 ...
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bioRxiv - Neuroscience 2021Quote: ... dissociated with Accutase and then transfected with one of the Tau or Tubulin pUCM vectors along with AAVS1 TALEN pairs using a Human Stem Cell Nucleofector Kit 1 (Lonza) with the Nucleofector 2b Device (Lonza) ...
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bioRxiv - Physiology 2022Quote: ... All human iPSC lines were negative for mycoplasma contamination as tested using the Mycoalert Mycoplasma testing kits (LT07-318, Lonza) and no karyotype abnormalities were found (KaryoStat+ ...
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bioRxiv - Developmental Biology 2020Quote: ... The guide RNA vector was then electroporated into the parent line containing the CRISPRi system using the Human Stem Cell Nucleofector Kit 1 solution with the Amaxa nucleofector 2b device (Lonza). Nucleofected cells were then seeded into a 6-well plate in mTeSR™-1 supplemented with Y-27632 (10 μM ...
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bioRxiv - Microbiology 2021Quote: ... Cells were stimulated by CD3/CD28 Abs for 2 days and nuclofected with 100 μM RORC or non-targeting (NT1) siRNA (ON-TARGETplus SMART pool, Dharmacon) using the Amaxa Human T cell Nucleofector Kit (Amaxa, Lonza), according to the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2020Quote: Adipose-derived human mesenchymal stem cells (AD-hMSCs) and dermal fibroblasts (DF) used in this study were purchased from Lonza. AD-hMSCs and DF were isolated from a 42-year-old ...
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bioRxiv - Genomics 2020Quote: Primary human dermal LECs and BECs were isolated from neonatal human foreskin as described previously88 and cultured in endothelial basal medium (EBM, Lonza) supplemented with 20% FBS ...
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bioRxiv - Microbiology 2021Quote: ... The human lung epithelial cell line Calu-3 (ATCC HTB-55) was maintained in DMEM F-12 (Lonza, Basel, Switzerland) supplemented with 10% FBS ...
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bioRxiv - Immunology 2020Quote: ... Activated cells were transfected using the Human T cell Nucleofector Kit (Amaxa Biosystem, #VPA-1002) and the Amaxa Nucleofector II system (Lonza), Program T-023 with ribonucleoprotein complexes ...
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bioRxiv - Immunology 2020Quote: ... Transfection of PBMCs: ASOs were electroporated into PBMCs using the Human T cell nucleofector kit (VPA-1002, Lonza, Basel, Switzerland). Transfection of TILs ...
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bioRxiv - Cell Biology 2023Quote: BCi-NS1.1 cells (passage 17-25) (kind gift of Dr. Ronald Crystal) and Normal Human Bronchial Epithelial cells (NHBE) (cat. no. CC-2541, Lonza, lot numbers 623950 ...
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bioRxiv - Immunology 2023Quote: ... at bead-to-cell ratio of 1:1 in human IL-2 medium (X-VIVO 15, serum-free hematopoietic cell medium, with 2mM L-Glutamine and gentamicin (Lonza) supplemented with 5% Human AB serum (Valley Medical) ...
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bioRxiv - Cancer Biology 2023Quote: ... The dissociated GSCs (1 × 106 cells) were resuspended in 100 μl of supplemented solution of the Human Stem Cell Nucleofector Kit 1 (Lonza) containing a combination of the px458 plasmid targeting each gene and the ssODN and then electroporated using B-016 program of Nucleofector 2b (Lonza) ...
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bioRxiv - Microbiology 2023Quote: ... amazonensis promastigotes using the Human T-Cell Nucleofector kit and the Amaxa Nucleofector electroporator (program U-033, Lonza, Basel, Switzerland), for integration into the 18S rRNA locus within the nuclear DNA ...
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Efficient Methods for Target Gene Manipulation in Haematopoietic Stem Cell Derived Human NeutrophilsbioRxiv - Cell Biology 2023Quote: ... CD34+ HSPC selected as previously described were preconditioned in SFEM II and StemSpan cytokines for 72 hours and electroporated with the Cas9-gRNP mixture using the Human CD34+ Cell Nucleofector kit (Lonza) in a Lonza IIb Nucleofector using program U-008 ...
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bioRxiv - Molecular Biology 2024Quote: ... All cell lines used are of human origin and were confirmed negative for mycoplasma before experimental use by using the MycoAlert Mycoplasma Detection Kit (Lonza) according to the the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2023Quote: ... and Cas9-GFP were then transfected into human iPSCs for INS mutation correction using embryonic stem cell Nucleofector Kit (VVPH-5012, Lonza). Transfected cells were cultivated in StemFlex medium (catalog #A3349401 ...
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bioRxiv - Physiology 2023Quote: Human lung microvascular ECs were purchased from ATCC and cultured in EBM-2 medium supplemented with EGM-2 BulletKit (Lonza). THP-1 and U937 cells were purchased from ATCC and cultured in RPMI medium 1640 (Corning ...
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bioRxiv - Synthetic Biology 2024Quote: ... negative selection of T cells was performed using the EasySep human T cell isolation kit (Stemcell) and cultured in X-VIVO 15 medium (Lonza) supplemented with 5% fetal bovine serum (FBS) ...
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bioRxiv - Immunology 2023Quote: ... of target genes in primary human T cells were generated by Cas9 ribonucleoprotein (RNP) electroporation using a 4D Nucleofector (Lonza) and P3 primary cell nucleofection reagents (Lonza ...