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Citations for Lonza :
851 - 867 of 867 citations for Polyhomeotic Like Protein 1 PHC1 Antibody FITC since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2020Quote: ... Conditioned media (containing EVs) was removed and the cells washed x 2 with PBS before trypsinisation using 1 × Trypsin-EDTA (Lonza, UK cat: T3924). Cell counts and viability were checked at the time of EV harvest using the trypan blue exclusion assay (0.4% Trypan blue solution ...
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bioRxiv - Microbiology 2022Quote: ... Each pellet was carefully resuspended in 20 μL of SF Cell Line Nucleofector Solution with Supplement 1 (Lonza Cat. #V4XC-2032, Basel, Switzerland) and 1.5 μL of pX458 plasmids (150 ng each of BAX g2 ...
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bioRxiv - Synthetic Biology 2024Quote: ... 1-5x105 iPSCs were resuspended in 20μL of P3 primary cell nucleofector solution plus supplement (cat.: V4XP-3032; Lonza Bioscience, Walkersville, MD, USA) along with the RNP complex and nucleofected using Lonza 4D Nucleofector (program CA-137) ...
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bioRxiv - Genetics 2024Quote: ... were transfected with 1 µg of plasmid DNA via electroporation using an AMAXA nucleofector kit following the manufacturer’s recommendation (Lonza Group Ltd., Basel, Switzerland). Plasmids included the empty pIRES-CD8 plasmid and pIRES-CD8 plasmids containing wild type MCOLN3 (MCOLN3WT ...
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bioRxiv - Cell Biology 2024Quote: ... and 5 μM of single-stranded donor oligonucleotides (ssODN) were delivered to 1×106 single cells by nucleofection using Lonza 4D nucleofection system (#V4XP-3024; Lonza Biologics, Slough, UK) according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2021Quote: ... electroporated with 10 μg of linearised plasmid DNA or PCR product using the Amaxa Basic Parasite Nucleofector Kit 1 and Nucleofector II device (Lonza, Switzerland, program X-001).
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bioRxiv - Microbiology 2020Quote: ... cells were collected by centrifugation and washed once with 50:50 TE and resuspended in 50ul SybrGreen (1:50 dilution with 50:50 TE; Lonza, CAT#12001-798, 10,000X) and incubated overnight at 4°C ...
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bioRxiv - Cancer Biology 2023Quote: ... The trachea was then exposed and a 21G needle was inserted to either 1) inflate the airway with 2cc of 1.5% low melt agarose (Lonza SeaPlaque™; Tm 65°C) for immediate fixation in cold 4% paraformaldehyde (Sigma ...
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bioRxiv - Biochemistry 2023Quote: ... Mm.Cas9.NOS2.1.AA) and 1 µM HiFi Cas9 enzyme (IDT, catalog no. 1081060) in 100 µL Nucleofector Solution V plus supplement (Lonza, catalog no. VCA-1003), using the preprogrammed electroporation protocol B-032 on Nucleofector II/2b ...
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bioRxiv - Neuroscience 2023Quote: ... 4−106 cells were pelleted at 300xg and then resuspended in 100 μL prewarmed nucleofector solution (Human Stem Cell Kit 1, Lonza, catalog no. VPH-5012); 4 μg of NGN2 plasmid and 1.5 μg each of 1R and 1L plasmids were added directly to the resuspended cells ...
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bioRxiv - Immunology 2023Quote: ... 2 million of CAR-T cells were electroporated with 1 μg Cas9 mRNA in 16-well Nucleocuvette™ Strips using EO-115 program (Lonza, per manufacturer’s instructions). Cells were expanded in human T cell media for another 3 days before analysis or transfer into mice.
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bioRxiv - Immunology 2023Quote: ... 2 million of CAR-T cells were electroporated with 1 μg Cas9 mRNA in 16-well Nucleocuvette™ Strips using EO-115 program (Lonza, per manufacturer’s instructions). Cells were expanded for another 2 days before transfer into NSG mice.
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bioRxiv - Synthetic Biology 2024Quote: ... were mixed at a 5:1 molar ratio and nucleofected using the CB-150 program and P3 primary reagents (Lonza, Cat. No. V4XP-3032) on a Lonza 4D nucleofector following manufacturer protocol ...
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bioRxiv - Bioengineering 2024Quote: ... and 800,000 cells were nucleofected with 2.5 µg of Super piggyBac Transposase (SBI cat. no. PB200A-1) and 10 µg of transposon plasmid using Lonza Cell Line Nucleofector Kit V (Lonza cat. no. VCA-1003) on an Amaxa IIb Nucleofector with program B-016 ...
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bioRxiv - Cell Biology 2021Quote: ... at a concentration of 10,000 units/l (penicillin) and 1 mg/l (streptomycin) and 2 mM L-glutamine (BioWhitaker, Lonza, 200 mM, cat. No. 17-605E), respectively ...
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bioRxiv - Neuroscience 2021Quote: ... DRGs were then mechanically triturated and washed in a complete neurobasal A medium (NBA, Gibco® Invitrogen™ supplemented with 2% B27, 2mM L-glutamine and 1% antibiotics: penicillin + streptomycin, Lonza™ BioWhittaker™), before being plated in 35- mm petri dishes ...
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bioRxiv - Neuroscience 2024Quote: ... A total of 0.5 µg per 106 of siRNA and 1 µg per 106 of DNA were transfected by Amaxa Nucleofector Technology using the Basic Nucleofector Kit (Lonza, Switzerland, EU, Cat# VPI-1001). After 18 h ...