Labshake search
Citations for Lonza :
801 - 850 of 1031 citations for Recombinant Human VAPB Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Physiology 2022Quote: ... The cells used were human marrow derived mesenchymal stem cells (hMSCs, Lonza, Slough, UK, cat No PT- 2507). In the EPL001-terminus study they were seeded at 10,000 cells per well ...
-
bioRxiv - Synthetic Biology 2022Quote: ... T cells were cultured in human T cell medium (HTCM) consisting of X-VIVO 15 (Lonza #04-418Q), 5% Human AB serum ...
-
bioRxiv - Developmental Biology 2022Quote: Primary human umbilical vein endothelial cells (HUVECs, passage 2) were thawed and cultured in EGM-2 media (Lonza). Media was replaced every 48h ...
-
bioRxiv - Neuroscience 2022Quote: ... 20,000 cells were resuspended in 100 μL nucleofection buffer from Human Stem Cell Nucleofector™ Kit 2 (Lonza). Salsa6f-AAVS1 SHL plasmid Template (2 μg ...
-
bioRxiv - Cell Biology 2022Quote: Human MSC were cultured in MSCBM™ Mesenchymal Stem Cell Basal Medium (MSCBM hMSC Basal Medium) (Lonza, UK) with necessary supplements (MSCGM hMSC SingleQuot Kit ...
-
bioRxiv - Cancer Biology 2021Quote: ... A human breast MCF10A cell line (CRL10317, ATCC) was grown with completed growth medium: MEGM Kit (Lonza CC3150) without gentamycin-amphotericin B mix (GA1000 ...
-
bioRxiv - Neuroscience 2022Quote: ... Human umbilical vein endothelial cells (HUVECs) were cultured in EBM-2 medium supplemented with EGM-2 SingleQuots (Lonza) on plates coated with rat tail collagen I (20 µg/cm2 ...
-
bioRxiv - Cell Biology 2022Quote: ... EGM-2 was then added to each well along with 200µl of normal human lung fibroblasts (CC2512, Lonza) at a concentration of 2×105 cells/ml ...
-
bioRxiv - Cancer Biology 2019Quote: ... Human breast epithelial cell line MCF10a (ATCC CRL- 10317) was cultured in complete MEGM media (Lonza, Walkersville, MD) with 100 ng/ml cholera toxin at 37°C in 5% CO2 ...
-
bioRxiv - Cancer Biology 2021Quote: A human breast MCF10A cell line (CRL10317, ATCC) was grown with completed growth medium: MEGM Kit (Lonza CC3150) without gentamycin-amphotericin B mix (GA1000 ...
-
bioRxiv - Developmental Biology 2021Quote: ... 2×106 iPSC single cells were transfected using the Amaxa Human Stem Cell Nucleofector® Kit 2 (Lonza) with 4 μg ...
-
bioRxiv - Bioengineering 2020Quote: Human bone marrow-derived mesenchymal stromal cells (MSCs) from a single donor were purchased from Lonza (Walkersville, MD). The trilineage potential of the cells was confirmed through induction in lineage-specific media ...
-
bioRxiv - Microbiology 2021Quote: ... The human lung epithelial cell line Calu-3 (ATCC HTB-55) was maintained in DMEM F-12 (Lonza) supplemented with 10% FBS ...
-
PSGL-1 inhibits HIV-1 infection by restricting actin dynamics and sequestering HIV envelope proteinsbioRxiv - Microbiology 2020Quote: ... the siRNAs were transfected into human CD4+ T cells using the Amaxa Nucleofector following the manufacturer’s protocols (Lonza). The siRNAs used in this study were purchased from GenePharma and the sequences are as below:
-
bioRxiv - Systems Biology 2022Quote: Human Dermal blood microvascular endothelial cells (DMECs) isolated from a single donor were purchased from Lonza (CC-2183). DMECs were cultured in EGM2-MV (Lonza ...
-
bioRxiv - Synthetic Biology 2024Quote: ... T cells were cultured in human T cell medium (hTCM) consisting of X-VIVO 15 (Lonza #04-418Q), 5% Human AB serum and 10 mM neutralized N-acetyl L-Cysteine (Sigma-Aldrich #A9165 ...
-
bioRxiv - Cell Biology 2023Quote: Human mammary epithelial cells (HMECs) were cultured at 37°C with 5% CO2 in MEBM basal medium (Lonza) supplemented with MEGM SingleQuots (Lonza ...
-
bioRxiv - Immunology 2023Quote: ... PBMCs were loaded in these wells in human T cell media (X-VIVO media, Lonza, Cat# 04-418Q) supplemented with 5% human AB serum ...
-
bioRxiv - Cell Biology 2023Quote: ... All human cell lines were tested for Mycoplasma contamination using a MycoAlert Mycoplasma Detection Kit (Lonza, Basel, Switzerland).
-
bioRxiv - Immunology 2023Quote: ... The MPRA vector library was nucleofected into TPP macrophages (5µg vector into 5×106 cells) in 100μl nucleofection buffer (Human Macrophage Nucleofection Kit, Lonza) using a Nucleofector 2b (program Y-011) ...
-
bioRxiv - Molecular Biology 2023Quote: Human embryonic kidney 293 (HEK293) cells (ATCC; CRL-1573) were grown in high-glucose DMEM (Lonza; BE12-604F) supplemented with 10% foetal bovine serum (FBS ...
-
bioRxiv - Immunology 2023Quote: ... beads were removed on day 3 followed by electroporation using the Human T Cell Nucleofector™ Kit (Lonza) and the Amaxa Nucleofactor® 2b (Lonza ...
-
bioRxiv - Developmental Biology 2023Quote: Human skeletal muscle myoblasts (CC-2561) and subcutaneous preadipocyte cells (PT-5020) were purchased from Lonza (Lonza Walkersville). Subcutaneous preadipocyte cells were induced to differentiate into terminal white adipocytes according to the manufacturer’s protocol.
-
bioRxiv - Biophysics 2023Quote: Human aortic endothelial cells (ATCC, Manassas, VA) were cultured in EGM-2 culture media (Lonza Walkersville, Basel, Switzerland) on 0.1% gelatin-coated plastic dishes until ∼80% confluence ...
-
bioRxiv - Immunology 2023Quote: ... PBMCs were loaded in these wells in human T cell media (X-VIVO media, Lonza, Cat# 04-418Q) supplemented with 5% human AB serum ...
-
bioRxiv - Microbiology 2024Quote: ... Human umbilical endothelial cells (HUVEC-C; ATCC, CRL-1730) were cultured in Lonza EGM-Plus (Lonza, CC-4542) supplemented with bullet kit (Lonza ...
-
bioRxiv - Cell Biology 2023Quote: ... S2Xpress cells were cultured in Insect-XPRESSTM protein-free medium (LONZA, Belgium) supplemented with 100 U/ml penicillin and 100 mg/ml streptomycin (GIBCO) ...
-
bioRxiv - Immunology 2023Quote: ... Protein expression was induced with 5µM CdCl2 in Insect-XPRESS medium (Lonza) for 5-7 days and supernatants were concentrated and purified using strep-tacting affinity chromatography columns in an AKTA FPLC system ...
-
bioRxiv - Molecular Biology 2024Quote: ... at 21°C in Insect Xpress Protein-free Insect Cell Medium (Lonza) supplemented with GlutaMAX (GIBCO ...
-
bioRxiv - Microbiology 2020Quote: ... Human neuroblastoma SH-SY5Y cells were grown in a 1:1 (v/v) mixture of EMEM with EBSS (Lonza) and Ham’s F12 (Gibco ...
-
bioRxiv - Evolutionary Biology 2021Quote: ... Human Stem Cell Nucleofector Kit 1 (Cat. No. VPH-5012) and mESC Nucleofector Kit (Lonza, Cat. No. VVPH-1001) were used for delivering DNA into human and mouse ESCs ...
-
bioRxiv - Molecular Biology 2019Quote: Wild type SV40-immortalized human fibroblasts (MRC5) were cultured in a 1:1 mixture of Ham’s F10 and DMEM (Lonza) supplemented with 1% antibiotics (penicillin and streptomycin ...
-
bioRxiv - Neuroscience 2019Quote: ... 8 × 105 cells were co-transfected with 3 µg plasmid-sgRNA construct and ssODN (0.6 µM final concentration) with Amaxa Human Stem Cell Nucleofector Kit 1 (Lonza) and program A-023 for the Amaxa Nucleofector Device II ...
-
bioRxiv - Cancer Biology 2020Quote: ... E4ORF1-transduced primary human umbilical vein endothelial cells (HUVECs) were cultured using the EGM-2 Bullet Kit (Lonza, Germany). All cell lines were kept at 37°C and 5% CO2 in a fully humidified incubator and negatively tested for mycoplasma by PCR.
-
bioRxiv - Immunology 2022Quote: ... and human monocytic leukemia cell line THP-1 (ATCC TIB-202) were cultured in RPMI-1640 (Lonza, 12-115F) containing 10% fetal bovine serum (FBS ...
-
bioRxiv - Developmental Biology 2020Quote: ... expressing Cas9 and single sgRNAs targeting upstream and downstream regions of the lncRNA promoter/locus were co-transfected into 1.5 × 106 H1 hESCs using the Human Stem Cell Nucleofector Kit 2 (Lonza) and the Amaxa Nucleofector II (Lonza) ...
-
bioRxiv - Microbiology 2021Quote: ... Pre-assembled Cas9-gRNA RNPs were electroporated into cells using Lonza Human T cell Nucleofector kit (Lonza, VPA-1002) and the Nucleofector2b equipment ...
-
bioRxiv - Immunology 2022Quote: NIH3T3 WT fibroblast cells (3T3) and human CD40L-expressing 3T3 (Urashima et al., 1996) were cultured in IMDM (Lonza) containing 10% FCS (Bodinco) ...
-
bioRxiv - Genetics 2019Quote: ... Human multipotent adipose-derived stem cells (hMADS) were cultured in growth medium which consisted of: DMEM (Lonza, BE12-707F), 10% FBS ...
-
bioRxiv - Immunology 2019Quote: ... washed once in PBS and resuspended in Mouse B cell Nucleofector Solution with Supplement (murine B cells) or Primary Cell Nucleofector Solution 3 with Supplement (human B cells) prepared to the manufacturer’s instructions (Lonza) at a concentration of 4 - 5 × 106 cells / 86 μL ...
-
bioRxiv - Microbiology 2020Quote: A549 cells (human lung carcinoma cell line, ATCC CCL-185) were grown in Dulbecco’s modified eagle medium (DMEM) (Lonza) supplemented with 10% heat-inactivated fetal bovine serum (FBS ...
-
bioRxiv - Genetics 2020Quote: ... 20 µg ssODNs and 4 µg pSpCas9(BB)-2A-GFP construct using Human Stem Cell Nucleofector Kit 2 (Lonza). For the generation of UE-RASGEF1A-int1-KO and PIK3C2B-int10-KO hPSC lines ...
-
bioRxiv - Cell Biology 2020Quote: ... Eriksson’s lab) and human osteosarcoma (U2OS) (ATCC HTB-96) cells were cultured in high-glucose DMEM (Lonza, BE12-119F), supplemented with 10% FBS (Gibco ...
-
bioRxiv - Genetics 2019Quote: PBT cells were transfected with 5 μg DNA for 5.106 cells in 100 μl of Human T Cell Nucleofector Solution (Lonza) with the program U-014 (Amaxa Biosystems).
-
bioRxiv - Microbiology 2021Quote: Human airway tracheobronchial epithelial cells isolated from airway specimens from donors without underlying lung disease were provided by Lonza, Inc ...
-
bioRxiv - Microbiology 2021Quote: Primary human bronchial epithelial cells (HBECs) derived from a non-smoking donor (Cat# CC-2540, male; Lonza; Donor 1) or derived directly from a patient at Cambridge University Hospitals NHS Trust (Research Ethics Committee Reference 19/SW/0152 ...
-
bioRxiv - Microbiology 2021Quote: Human airway tracheobronchial epithelial cells isolated from airway specimens from donors without underlying lung disease were provided by Lonza, Inc ...
-
bioRxiv - Cell Biology 2022Quote: Human umbilical vein endothelial cells (HUVEC) were cultured between passage 3 and 6 in EGM-2 complete medium (Lonza)3,52–55 ...
-
bioRxiv - Neuroscience 2022Quote: Primary human astrocytes were cultured on Matrigel-coated (0.08 mg/well) 6-well plates in astrocyte growth medium (Lonza). On the day of plating ...
-
bioRxiv - Cancer Biology 2022Quote: ... HEK293T cells (human embryonic kidney; LGC Nordic ATCC CRL-3216) were cultured in DMEM high glucose (#BE12-614Q, Lonza), supplemented with 2 mM L-glutamine ...