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Citations for Lonza :
8201 - 8250 of 9615 citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2020Quote: ... After red blood cell lysis with ammonium-chloride-potassium lysis buffer (Lonza), cells were incubated for 4 hours at 37° C with 5% CO2 in a nontissue culture (TC ...
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bioRxiv - Developmental Biology 2020Quote: Nucleofection (NSCs) was performed using the AMAXA Nucleofection Technology (Lonza, VCA-1003). Lipofection (ESCs ...
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bioRxiv - Cancer Biology 2020Quote: ... Cultured cells were regularly tested for mycoplasma using the mycoAlert Detection Kit (Lonza).
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bioRxiv - Molecular Biology 2020Quote: ... 1 x 106 MLN cells were cultured in X-vivo 15 medium (Lonza) supplemented with 1% L-glutamine (Invitrogen) ...
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bioRxiv - Molecular Biology 2020Quote: ... Residual endotoxin was assessed by using an Endotoxin Standards kit (Lonza, Anaheim, USA) to ensure there was no LPS contamination in the SEA extracted from control and AChE-KI eggs) ...
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bioRxiv - Immunology 2020Quote: ... Spleens were incubated with ammonium chloride potassium lysing buffer (Lonza) for 3 min at room temperature to lyse red blood cells ...
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bioRxiv - Immunology 2020Quote: ... All cell lines were tested and confirmed to be Mycoplasma and endotoxin-free using the MycoAlert Detection kit (Lonza) and the Endosafe-PTS system (Charles River Laboratories) ...
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bioRxiv - Immunology 2020Quote: ... RPMI 1640 (Lonza) containing 0.292 ng/ml glutamine ...
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bioRxiv - Molecular Biology 2020Quote: ... 100U/ml penicillin and 100mg/ml streptomycin (Lonza). For doxycycline treatment 200 ng/ml Doxycycline (Sigma ...
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bioRxiv - Molecular Biology 2020Quote: hTERT-immortalized Retinal Pigment Epithelial cells (RPE-1) were cultured in DMEM-F12 (Lonza) containing 10% FBS (Bodinco) ...
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bioRxiv - Immunology 2020Quote: ... Leukocytes were washed thoroughly in PBS buffer (without Ca+2 and Mg+2) (Lonza, Walkersville, MD) and cell viabilities were assessed using trypan blue exclusion ...
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bioRxiv - Synthetic Biology 2020Quote: ... and 1 mM sodium pyruvate (Lonza). Polyethylenimine (PEI ...
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bioRxiv - Molecular Biology 2020Quote: ... The plug and 600 ng of Lambda Hind III DNA markers were separated by electrophoresis on a 0.4% agarose gel (SeaKem Agarose LE, Lonza) in 1× TBE buffer at 1.32 V/cm for 14 hr at room temperature with buffer circulation in a Sub-cell GT electrophoresis system (15 × 20 cm gel ...
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bioRxiv - Neuroscience 2020Quote: ... All lines were routinely tested for mycoplasma infection (MycoAlert, Lonza).
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bioRxiv - Neuroscience 2020Quote: ... Cells were routinely tested to confirm the absence of mycoplasma contamination (Lonza MycoAlert). CRISPR-Cas9 based genome engineering experiments were carried out as previously described (35–37) ...
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bioRxiv - Neuroscience 2020Quote: ... and 1% penicillin/streptomycin (Lonza, 17-602E). Cells were transitioned to serum-free DMEM-based media for collection of conditioned media from both lines ...
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bioRxiv - Neuroscience 2020Quote: ... the DMEM-based culture medium was replaced with Minimum Essential Eagle’s Medium (EMEM; Lonza, 12-125F) containing 2.38 mM sodium bicarbonate (Sigma ...
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bioRxiv - Neuroscience 2020Quote: ... cells were transfected with the Amaxa Mouse Neuron Nucleofector Kit (Lonza, VPG-1001) using the Amaxa Nucleofector II device (Lonza) ...
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bioRxiv - Neuroscience 2020Quote: ... Human Brain Microvascular Endothelial Cells (HBMECs) (Alphabioregen-CliniSciences, Nanterre, France) were cultured in endothelial basal medium-2 (EBM-2) supplemented with EGM™-2 BulletKits™ (Lonza, Bäle, Switzerland). Cell from passage 3 to passage 6 were used ...
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bioRxiv - Neuroscience 2020Quote: ... non-essential amino acids 2 % (Lonza, Bäle, Switzerland), FGF 1 ng/mL (PeproTech ...
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bioRxiv - Neuroscience 2020Quote: ... Cell media was collected and the presence of adenylate kinase was measured with the ToxiLightTM cytotoxicity assay kit (Lonza, Basel, Switzerland) to check the integrity of the cell membrane ...
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bioRxiv - Neuroscience 2020Quote: ... 500 µl of cell media was collected and the presence of adenylate kinase was measured with the ToxiLightTM cytotoxicity assay kit (Lonza, Basel, Switzerland) to check the integrity of the cell membrane ...
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bioRxiv - Synthetic Biology 2020Quote: Human Umbilical Vein Endothelial Cells (HUVECs) (Lonza, Germany) were grown on 0.1% gelatin-coated 35mm cell culture dish in EGM2 media (20% Fetal Bovine Serum ...
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bioRxiv - Biochemistry 2020Quote: Chinese hamster ovary DG-44 cells (Thermo Fischer Scientific) were cultured in the ProCHO 5 medium (Lonza, Switzerland), supplemented by 4 mM glutamine ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... and were cultured in DMEM (Lonza) supplemented with 10% heat inactivated fetal calf serum ...
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bioRxiv - Immunology 2020Quote: ... Red blood cells were removed by treatment with a hypotonic lysis buffer (Lonza). The cells were resuspended in FACS buffer (HBSS/ 1% FBS ...
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bioRxiv - Cancer Biology 2020Quote: ... supplemented with endothelial cell growth medium (EGM™ SingleQuots™, Lonza), 10% of FBS ...
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bioRxiv - Cancer Biology 2020Quote: ... were maintained in endothelial cell basal medium (EBM™, Lonza) supplemented with endothelial cell growth medium (EGM™ SingleQuots™ ...
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bioRxiv - Cancer Biology 2020Quote: Human umbilical vein endothelial cells (HUVEC, Lonza) were maintained in endothelial cell basal medium (EBM™ ...
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bioRxiv - Genetics 2020Quote: ... 2×105 UE control hPSCs were transfected with a pair of pSpCas9(BB)-2A-GFP constructs containing the specific sgRNAs (350 ng per construct) using P3 Primary Cell 4D-Nuclecfector X Kit (Lonza). The transfected cells were plated in Matrigel-coated dish and cultured for 2 days ...
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bioRxiv - Genetics 2020Quote: ... 20 µg ssODNs and 4 µg pSpCas9(BB)-2A-GFP construct using Human Stem Cell Nucleofector Kit 2 (Lonza). For the generation of UE-RASGEF1A-int1-KO and PIK3C2B-int10-KO hPSC lines ...
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bioRxiv - Immunology 2020Quote: ... and CDR3B gRNA (10 nmol) were used to transfect 1×108 TnT cells using the 4D-Nucleofector LV unit (Lonza, #AAF-1002L). TnT cells with restored CD3 surface expression were bulk-sorted (SEL 1 ...
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bioRxiv - Immunology 2020Quote: ... The inoculum was then removed and replaced with 2 mL of complete DMEM medium with 1% SeaPlaque agarose (Lonza, Cat# 50100). The plate was incubated at 37°C and 5% CO2 for 3 days ...
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bioRxiv - Immunology 2020Quote: ... Transfection of primary T cells with Cas9 RNP complexes and TCRβα HDR templates was performed 3-4 days following activation using the 4D-Nucleofector and a 20 uL format P3 Primary Cell kit (Lonza, V4XP-3032). Briefly ...
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bioRxiv - Immunology 2020Quote: Human umbilical vein endothelial cells (ECs) were obtained from Lonza and cultured in EGM-2 complete culture medium (Lonza) ...
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bioRxiv - Immunology 2020Quote: ... HEK293T cells were routinely tested and found negative by MycoAlert™ mycoplasma detection kit (Lonza Nottingham, Ltd.). HEK293T cells were plated into cell chambers of 10 trays in one litre of complete medium and were transfected with DNAS-EV expression plasmid using PEI ...
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bioRxiv - Immunology 2020Quote: ... were obtained from Lonza and cultured in EGM-2 complete culture medium (Lonza). ECs were seeded at 5000 cells/cm2 and cultured to at least 70% confluence before co-culture with CD4+ T cells.
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bioRxiv - Immunology 2020Quote: ... in 10% heat inactivated human serum with RPMI (Lonza) at a concentration of 2 × 10^7/ml ...
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bioRxiv - Immunology 2020Quote: ... and used to infect Trichoplusiani ni (High Five) cells grown in InsectXpress media (Lonza, #BELN12-730Q) for protein expression ...
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bioRxiv - Immunology 2020Quote: ... All transfection experiments were performed with Amaxa Cell Line Nucleofector Kit V for monocytic cells(Lonza, Germany) by using Amaxa Electroporation System (Amaxa Inc ...
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bioRxiv - Immunology 2020Quote: ... and pmaxGFP (0.5 ug; Amaxa Noclecfector Kit V for THP-1, Lonza). All transfection experiments were performed with Amaxa Cell Line Nucleofector Kit V for monocytic cells(Lonza ...
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bioRxiv - Developmental Biology 2020Quote: ... HLECs were grown on culture dishes or glass slide coated with 0.2% gelatin and were maintained in EGM-2 EC Growth Medium-2 Bullet Kit (Lonza). All experiments were conducted using cells until passage (P ...
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bioRxiv - Cancer Biology 2020Quote: ... Stable pools of cells were maintained in small airway growth medium (Lonza).
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bioRxiv - Cancer Biology 2020Quote: 1×105 cells were suspended in 1 ml of 0.33% select agar in small airway growth medium without EGF (Lonza) and plated on a bottom layer of 0.5% select agar in the same media in six-well dishes ...
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bioRxiv - Physiology 2020Quote: ... 0,147 μg/mL TLCK (Lonza) and 10 μg/mL DNAse 1 (Roche) ...
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bioRxiv - Immunology 2020Quote: ... hEGF (Lonza), preservative-free heparin 10 IU/mL (Biochrom) ...
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bioRxiv - Cell Biology 2020Quote: ... Cells were grown in flasks in Dulbecco’s Modified Eagle’s Medium (DMEM; Lonza, Basel, Switzerland) with 1 g/L D-glucose ...
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bioRxiv - Pathology 2020Quote: Human coronary artery endothelial cells (HCAECs) from Lonza, Switzerland were cultured in endothelial cell basal medium (EBM ...
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bioRxiv - Cancer Biology 2020Quote: ... Stable overexpression of HIP1 was generated by transfection using Nucleofector technology (Lonza). Stable knockdown of HIP1 was generated by nucleofection (Lonza ...
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bioRxiv - Cancer Biology 2020Quote: ... Stable knockdown of HIP1 was generated by nucleofection (Lonza) of the two shHIP1 constructs (Origene ...