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Citations for Lonza :
601 - 650 of 822 citations for Tryptic Soy Agar TSA CRITERION Dehydrated Culture Media since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2023Quote: ... The supernatant medium from cell cultures was analyzed monthly for the presence of mycoplasma using MycoAlert PLUS (Lonza) or via polymerase chain reaction (PCR).
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bioRxiv - Cell Biology 2022Quote: ... Cell cultures were washed with Dulbecco’s phosphate buffered saline without calcium or magnesium (DPBS-; Lonza, Walkersville, MD, USA) and detached with 0.25% trypsin with 0.53 mM EDTA (ATCC ...
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bioRxiv - Cell Biology 2024Quote: ... expanded in submerged culture for one or two passages in bronchial epithelial growth medium (BEGM, Lonza, Walksville, MD), and then seeded on Transwell membranes (Corning ...
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bioRxiv - Neuroscience 2024Quote: ... Lymph node cells were dissociated and kept in culture with X-vivo 15 (02-053Q, Lonza, Basel, Switzerland) supplemented with 10% fetal bovine serum for 24 h ...
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bioRxiv - Cancer Biology 2024Quote: ... The cell culture was checked for mycoplasma contamination with the MycoAlert® Mycoplasma Detection Kit (LT07-118, Lonza) according to the manufacturer’s guidelines ...
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bioRxiv - Cell Biology 2021Quote: Whole-mount Drosophila ovary samples (approximately 5 flies per experiment) were dissected into Grace’s insect media (Lonza, Walkersville, MD) and fixed for 10 minutes at room temperature in 4% paraformaldehyde in Grace’s insect media ...
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bioRxiv - Biochemistry 2020Quote: ... Large-scale suspension cultures (300 mL) of High Five insect cells at 0.5 × 106 cells/ml were grown in Insect-Xpress media (Lonza) and inoculated with P2 baculovirus solution containing the Rpc5 constructs ...
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bioRxiv - Cell Biology 2021Quote: ... PBMCs were seeded onto collagen I-coated wells at a cell density of 1.5 - 2 × 106 cells/cm2 in endothelial growth media 2 (EGM-2) medium (Lonza) supplemented with 16% defined fetal bovine serum (FBS ...
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bioRxiv - Microbiology 2021Quote: CHO cells stably expressing recombinant RBD of SARS-CoV-2 Spike (aa. 319-591) (GenBank accession no. AAP13567.1) were propagated in ProCho5 media (Lonza) containing glutamine ...
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bioRxiv - Immunology 2022Quote: ... primary human CD8+ T cells were thawed into complete human T cell media: X-VIVO 15 (Lonza # 04-418Q), 5% Human AB serum (Valley Biomedical #HP1022 ...
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bioRxiv - Immunology 2020Quote: ... DCs were seeded in a 24-well plate at a density of 1× 106 cells/well on 1.5mm coverslips (Warner Instrument) and cultured in complete phenol-red free RPMI 1640 media (Lonza) for 30 minutes at room temperature ...
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bioRxiv - Microbiology 2021Quote: ... 2x overlay media was added to the inoculum to give a final concentration of 2% (v/v) FBS / MEM media and 0.4% (w/v) SeaPrep Agarose (Lonza) to achieve a semi-solid overlay ...
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bioRxiv - Immunology 2022Quote: Cryopreserved CAR T cells produced from heathy donors in the Seattle Children’s GMP production facility were thawed and cultured overnight in XVIVO media (Lonza) supplemented with IL-2 at 4.6ng/ml ...
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bioRxiv - Cell Biology 2021Quote: Tracheal cells (60,000-100,000 cells/ well in 96 well plate) were cultured in small airway growth media (SABMTMBasal Medium, CC-3119 Lonza) containing 1× insulin ...
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bioRxiv - Neuroscience 2023Quote: ... containing ∼1000 neurons) was transferred to 9 mL of wash media (DMEM/F12 with penicillin/streptomycin [Lonza; Allendale, NJ]), then recovered by centrifugation (∼350 xG ...
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bioRxiv - Bioengineering 2022Quote: A total of 500,000 CD34+ hiPSC-EPs were centrifuged twice and resuspended in Complete Endothelial Growth Media 2 (EGM-2, Lonza) supplemented with 50 ng/mL recombinant human vascular endothelial growth factor (VEGF ...
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bioRxiv - Bioengineering 2022Quote: ... primary hepatocytes were seeded 400,000 cells per well in complete hepatocyte plating media (HCM kit, Lonza, Cat# CC-3198). After four hours of incubation at 37 °C and 5 % CO2 ...
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bioRxiv - Immunology 2022Quote: ... PBMC were seeded at 1.5×106 cells per cm2 in X-VIVO 10 media (Cat. No. BE04-743Q, Lonza) +1% penicillin-streptomycin (Cat ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: NIH-3T3 cells were grown in complete NIH-3T3 media (DMEM with 4.5g/L Glucose and 4mM L-Glutamine (Lonza), 10% ...
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bioRxiv - Genetics 2023Quote: ... IMR90 fetal lung fibroblasts at passage 7 were cultured in FGM-2 lung fibroblast basal media (Lonza, #CC-3131) according to the manufacturer’s instructions.
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bioRxiv - Biochemistry 2024Quote: ... the media supernatant was removed and we washed the cells with 2 ml of prewarmed 1xDPBS (Lonza, 17-512F). The PBS was then removed and cells were then incubated at 37°C in 250 μL of trypsin (Corning ...
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bioRxiv - Genetics 2024Quote: ... 1.25 × 104 HRCE cells were plated in a 96-well plate in renal epithelial growth media (Lonza, CC-3190). 24 hours later cells were transfected with 50ng regulatory element plasmid and 50ng Renilla plasmid (pGL 4.74 ...
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bioRxiv - Biochemistry 2024Quote: ... Viral amplification was achieved by P1 infection of a 25ml culture of Sf9 cells growing in suspension at a cell density of 0.5x106 cells/ml in Insect-XPRESS media (Lonza). Cells were incubated at 27°C at 130 r.p.m ...
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bioRxiv - Biochemistry 2024Quote: ... Large-scale protein expression was carried out through addition of 2ml of P2 viral stock to 500ml of High Five cells grown to 0.5x106 cells/ml in Insect-XPRESS media (Lonza) in a roller bottle flask (Greiner) ...
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bioRxiv - Bioengineering 2024Quote: ... or no antigen (K562-mKate2) at a 1:1 Target:T cell ratio in 250 μl X-VIVO 15 media (Cat: 02-053Q, Lonza), supplemented with 5% human AB serum (Gemini Bio) ...
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bioRxiv - Genetics 2024Quote: ... Naïve T cells were cultured in basal media comprised of X-VIVO 15 Serum-free Hematopoietic Cell Medium (Lonza) with 5% FBS (v/v ...
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bioRxiv - Bioengineering 2022Quote: NK-92 cell line was acquired from the American Type Culture Collection and maintained in X-VIVO 10 (Lonza) supplemented with 20% FBS and 0.02 mM folic acid (Millipore Sigma ...
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bioRxiv - Immunology 2021Quote: ... density gradient centrifugation (20 min, 800g) and culture for 12-18 h in X-VIVO 10 TM medium (Lonza) supplemented with gentamicin ...
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bioRxiv - Microbiology 2021Quote: ... 500 EBs were transferred per T175 tissue culture flasks in 20 mL X-VIVO™15 (04-418Q, Lonza), supplemented with 100 ng mL-1 M-CSF (PHC9504 ...
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bioRxiv - Bioengineering 2021Quote: ... were used between passages 3-5 for paxillin experiments and culture medium was changed every 2-3 days (Lonza FBM Basal Medium supplemented with 2 v/v% fetal bovine serum (FBS) ...
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bioRxiv - Neuroscience 2020Quote: ... EBs were collected and transferred into 6-well cell culture plate (12-16 EBs/well) in X-VIVO15 (Lonza) supplemented with IL-3 ...
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bioRxiv - Neuroscience 2022Quote: ... culture conditions and characterization: The isolated epidermis was placed in a petri dish containing HBSS buffer (Lonza, # CC-5022) for 10 minutes at room temperature ...
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bioRxiv - Cancer Biology 2022Quote: ... Cells were maintained in culture for the indicated time in the following medium: X-VIVO 10 (Lonza, BE04-380Q) supplemented with 20% BIT 9500 Serum Substitute (StemCell technologies ...
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bioRxiv - Cancer Biology 2020Quote: ... were used in passages 3-6 and cultured on 0.1% gelatin-coated tissue culture plates in complete EGM2 (Lonza) supplemented to a total of 10% Fetal Bovine Serum (FBS) ...
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bioRxiv - Biophysics 2020Quote: SARS-CoV-2 was isolated from the oropharyngeal swab of a laboratory-confirmed COVID-19 patient in Hungary and passed two times in VeroE6 cell line (European Collection of Authenticated Cell Culture, Salisbury, U.K.) in Dulbecco’s Modified Eagle’s Medium (Lonza, Basel, Switzerland) supplemented with 5% fetal bovine serum (EuroClone ...
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bioRxiv - Immunology 2021Quote: ... cells were washed with PBS and 1 ml of fresh Hepatocyte Culture Medium (HCM) was added per well (Lonza). At 6 or 72 hpi ...
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bioRxiv - Immunology 2021Quote: ... Cells were centrifuged for 5 min at 1200 rpm at 20°C and resuspended at 2.5×106 cells/ml in X-VIVO 15 cell culture medium (Lonza) with 50 U/ml penicillin–streptomycin ...
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bioRxiv - Bioengineering 2023Quote: ... cAP-0001GFP) were cultured in 0.2% gelatin-coated T75 tissue culture flasks with EGM-2 medium (Lonza, CC-3162). The medium was changed every other day ...
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bioRxiv - Neuroscience 2023Quote: ... We regularly monitored all cell cultures for mycoplasma contamination using the MycoAlert™ Mycoplasma Detection Kit (Lonza, #LT07-218).
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bioRxiv - Molecular Biology 2024Quote: ... once the cell confluency reached 90% the culture medium was changed to 10 mL XVIVO-10 medium (Lonza #(BE)BP04-743Q ...
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bioRxiv - Microbiology 2022Quote: ... and neonatal dermal microvascular endothelial cells (hDMVEC) were maintained as monolayer cultures in EBM-2 medium (Lonza, Walkersville, USA) supplemented with 5% fetal bovine serum ...
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bioRxiv - Microbiology 2022Quote: ... NHBE cells from a single donor were amplified and differentiated into air-liquid interface cultures as recommended by Lonza and described in (49) ...
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bioRxiv - Developmental Biology 2024Quote: ... Mycoplasma testing was carried out by DAPI staining of fixed cultures and using the mycoalert mycoplasma detection kit (Lonza). We confirm that the cell lines used in this study were free of mycoplasma and aerobic bacteria and fungi.
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bioRxiv - Neuroscience 2024Quote: ... Presence of mycoplasma in culture was assessed using the MicoAlert assay control test according to the manufacturer’s instructions (cat #LT07-518, Lonza). Sanger sequencing was performed to verify the presence of pathogenic variant in patient cell lines (clones 11 and 13 ...
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bioRxiv - Genomics 2024Quote: ... Mycoplasma contamination testing was routinely performed in the cell lines while in culture (MycoAlert™ Mycoplasma Detection Kit, Lonza). LNCaP cells were seeded at 80% confluence and grown for 3 days in phenol red-free RPMI Medium with 5% charcoal-stripped Fetal Bovine Serum (FBS ...
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bioRxiv - Cancer Biology 2024Quote: ... All cell cultures were routinely tested for the presence of mycoplasma using the MycoAlert Mycoplasma Detection Kit (Lonza, USA) following manufacturer’s protocol.
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bioRxiv - Cancer Biology 2024Quote: ... The cell cultures were regularly checked for mycoplasma contamination with the MycoAlert® Mycoplasma Detection Kit (LT07-118, Lonza) according to the manufacturer’s guidelines ...
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bioRxiv - Genomics 2020Quote: LNCAP (ATCC) and PC3 (ATCC) cells were cultured and maintained in RPMI media supplemented with 10% fetal bovine serum (FBS; Lonza) 1mM sodium pyruvate (Gibco ...
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bioRxiv - Cancer Biology 2020Quote: ... William Hahn (Dana-Farber Cancer Institute) and were cultured in small airway epithelial growth media (SAGM) with SAGM supplements and growth factors (PromoCell or Lonza). All cells were maintained at 37°C in 5% CO2 and confirmed mycoplasma-free.
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bioRxiv - Immunology 2021Quote: ... of four genetically independent donors were grown as monolayers in 100% humidity and 5% CO2 at 37 °C in serum-free defined growth media (BEGM, Lonza). NHBEs (passage 3 ...