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Citations for Lonza :
451 - 500 of 510 citations for Sodium hypochlorite solution cp since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genomics 2023Quote: ... and 120 pmol of in vitro synthesized gRNA were electroporated into 250,000 MCF7 cells with the P3 primary nucleofection solution (Lonza, V4XP-3024), using the DN-100 Lonza 4D-Nucleofector program ...
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bioRxiv - Genetics 2023Quote: All iPSC lines were passaged as clumps or single cells upon reaching 80-85% confluency using Versene (0.02% EDTA solution, Lonza #17-711E) or StemPro Accutase (Gibco #A1110501) ...
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bioRxiv - Biochemistry 2024Quote: ... 5.0 × 106 OSCs were mixed with 200 pmol of siRNAs in 20 μL of Solution SF of the Cell Line Nucleofector Kit SF (Lonza Bioscience) and electroporation was performed using a Nucleofector 96-well Shuttle device (Lonza Bioscience) ...
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bioRxiv - Molecular Biology 2024Quote: ... 2 μL of the final 100 μM siRNA duplex was mixed with 100 μL of Nucleofection solution V (Lonza, VVCA-1003) and transfected into 10 million cells using a NucleofectorTM 2b Device and program T-029 (Lonza) ...
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bioRxiv - Cancer Biology 2024Quote: ... 20 µL of cell suspension (200,000 cells) was mixed with the RNP-containing solution and electroporated using the 4D-Nucleofector X Unit (Lonza, Basel, Switzerland) with the CM138 nucleofection program ...
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bioRxiv - Immunology 2024Quote: ... The DPP4 KO and NTC groups were washed with 1X PBS and resuspended with P3 primary nucleofection solution (Lonza, cat. V4XP3012) containing ribonucleoprotein (RNP ...
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bioRxiv - Molecular Biology 2024Quote: ... 2 μL of the final 100 μM siRNA duplex was mixed with 100 μL of Nucleofection solution V (Lonza, VVCA-1003) and transfected into 4 million cells (48 h knockdown ...
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bioRxiv - Physiology 2021Quote: ... were mixed with 1.49 μL of ssODN (100 μM) and Cas9 complex consisting of 18 μL SF 4D-nucleofector X solution + supplement1 (Lonza V4XC-2012), 6 μL of sgRNA (30 pmol/μL) ...
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bioRxiv - Microbiology 2022Quote: ... Cells that were harvested at 48 hours were first washed by dispensing and aspirating 37°C HEPES buffered saline solution (Lonza, CC-5022), and then trypsinized with 0.025% Trypsin/EDTA (Lonza ...
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bioRxiv - Bioengineering 2022Quote: ... supplemented with 10% (v/v) fetal bovine serum (FBS, Biochrom AG, Berlin, Germany) and 1% (v/v) antibiotic–antimycotic solution (AAS, Lonza, Basel, Switzerland) was used for culturing human dermal fibroblasts (HDF ...
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bioRxiv - Immunology 2020Quote: ... 50 pmol sgRNA and 20 pmol Cas9 were combined and diluted to 20 µL with nucleofection solution P1 or P3 (with supplement added, Lonza, Basel, Switzerland). The mixture was incubated at 25 °C for 10 min or up to 2 h and immediately used to electroporate moDCs ...
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bioRxiv - Genomics 2021Quote: ... mESCs in 6-cm dishes were transfected with 7.2 μg pX330-Cas9 plus 1.8 μg GFP expression vector by 4D-nucleofector X (Lonza, solution Cytomix, program GC104), then harvested for genomic DNA 3 days after transfection.
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bioRxiv - Neuroscience 2020Quote: ... dissociated ganglia were pelleted at 100 x g for 5 min and resuspended in 100 µl ‘Nucleofector solution’ (Rat Neuron Nucleofector kit; Lonza, Alpharetta, GA). 4-6 μg of plasmid was electroporated using the AMAXA Nucleofector device (Neurons Rat DRG ...
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bioRxiv - Bioengineering 2022Quote: ... Hydrogels were fabricated using a solution consisting of lyophilized GelMA (5 wt%) dissolved at 37°C in phosphate buffered saline (PBS; Lonza 17-516F) and combined with 0.1% w/v lithium acylphosphinate (LAP ...
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bioRxiv - Cancer Biology 2022Quote: ... 2 × 105 single cells were resuspended with Lonza P3 Primary Cell Nucleofector® Solution and mixed with the pre-formed RNP complexes and transferred to a Nucleocuvette™ (Lonza). Nucleofection was performed using program EO-115 on the 4D Nucleofector™ X unit (Lonza) ...
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bioRxiv - Systems Biology 2024Quote: ... Dissociated cells were aspirated using a wide-bore pipette tip and placed in a tube containing ice-cold Trypsin Neutralization Solution (Lonza, CC-5002); this was repeated to maximize cell collection ...
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bioRxiv - Immunology 2023Quote: ... T cells were then rinsed with PBS and 10 × 106 cells were resuspended in 20 µl of P4 primary cell nucleofection solution (P4 Primary Cell 4D-Nucleofector X Kit S, Lonza V4XP-4032). 20 μL of resuspended T cells were then gently mixed with 5 µL of RNP complex and incubated for 2 minutes at room temperature ...
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bioRxiv - Cell Biology 2024Quote: ... and sgRNAs (see Key Resources Table) were delivered by nucleofection to a single cell suspension of 105 iBCs in Primary Cell P3 solution (Lonza, #V4XP-3032). Cells were re-cultured and the resulting clonally-derived spheroids were isolated ...
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bioRxiv - Cell Biology 2024Quote: ... Cells were then passaged as previously described and 8×105 cells were resuspended in 100 µl of nucleofector solution (Lonza, VPH-5012). Depending on the experiment ...
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bioRxiv - Cell Biology 2023Quote: ... Approximately 1.0*106 iPSCs were gathered as single cells as described under ‘cell lines and cell culture’ and resuspended in 100 μL NucleofectorTM Solution V (Lonza, Basel, Switzerland) and added to the reaction mixture ...
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bioRxiv - Developmental Biology 2024Quote: ... Cells were resuspended in 20 µl nucleofection buffer (16.4 µl Nucleofector® Solution + 3.6 µl Supplement) provided in P3 Primary Cell 4D-Nucleofector™ X Kit S (Lonza, V4XP-3032). After the addition of 3 µl RNP and 0.5 µl of Alt-R Cas9 Electroporation Enhancer (IDT ...
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bioRxiv - Biochemistry 2024Quote: 1 µL of 63 µM Alt-R™ S.p.Cas9 V3 (IDT: 10007807) was diluted with 18 µL of SE Cell Line Nucleofector® Solution (Lonza V4XC-1032), followed by the addition of 1.8 µL of 100 µM sgRNA (3:1 sgRNA:Cas9) ...
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bioRxiv - Molecular Biology 2022Quote: ... 480 pmol L2 gRNA targeting BFP and 500 pmol BFP to GFP ssODN template using CM-189 and SF solution (Lonza, 4D electroporator) per replicate ...
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bioRxiv - Cell Biology 2023Quote: ... The linearized construct and the purified schizonts were mixed with Nucleofector solution from an Amaxa human T cell Nucleofector Kit and electroporated using the Amaxa Nucleofector II device (Lonza, Köln, Germany). Directly after transfection 50 µl RPMI-1640 complete was added to the transfection reaction followed by intravenous injection into one SWISS mouse ...
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bioRxiv - Microbiology 2023Quote: Colons from infected mice were open longitudinally and gently washed with ice-cold 1X PBS before being placed in Hanks’ balanced salt solution (HBSS) with phenol red and without calcium and magnesium (Lonza, Basel, Switzerland) on ice ...
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bioRxiv - Genomics 2023Quote: ... were washed twice with PBS and resuspended in a solution containing a 4.5:5 mixture of nucleofection buffer (Buffer SE, Lonza Lot #: S-09279) and supplement (Lonza ...
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bioRxiv - Molecular Biology 2023Quote: ... were mixed with 1.49 µL of ssODN (100 µM) and Cas9 complex consisting of 18 µL SF 4D-nucleofector X solution + supplement1 (Lonza V4XC-2012), 6 µL of sgRNA (30 pmol/µL) ...
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bioRxiv - Cell Biology 2023Quote: ... RPE1 cell pellets containing 1×10^6 cells were resuspended in Nucleofection Solution for Primary Mammalian Epithelial Cells (Lonza cat# VPI-1005) in the presence of nucleofection enhancer (cat# 1075915 ...
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bioRxiv - Cell Biology 2024Quote: ... 8 x 105 H9 hESCs in single-cell suspension were resuspended in 100 µL electroporation buffer from the Human Stem Cell Nucleofector Solution 2 kit (Lonza, VPH-5022). Alt-R Cas9 Electroporation enhancer (1.1 µM ...
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bioRxiv - Microbiology 2024Quote: One million K562 cells were electroporated with 3 μg of DENV2-luciferase replicon [38] (provided by Jan Carette, Stanford University) in 100 μL SF Cell Line Nucleofector solution (Lonza V4XC-2012) using pulse code FF-120 (Amaxa 4D Nucleofector ...
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bioRxiv - Cell Biology 2024Quote: ... the Ribonucleoprotein (RNP) complexes (9:1 sgRNA to Cas9 ratio) were assembled in Nucleofector Solution plus Supplement (Lonza Amaxa HMEC Nucleofector Kit) to a total volume of 100µL ...
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bioRxiv - Genetics 2024Quote: ... erythroleukemia K562 cells were washed in 50 mL PBS at RT and approximately 1×106 cells were resuspended in SF solution (Lonza V4XC-2032) and electroporated in presence of pre-assembled Cas9:gRNA complexes plus 12 µg ssDNA (100 µL format ...
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bioRxiv - Genetics 2024Quote: ... CD34+ HSPCs pre-stimulated for two days were washed in 50 mL PBS at 37° C and resuspended in P3 nucleofector solution at RT (Lonza, V4XP-3032). Approximately 2.5×105 CD34+ HSPCs were mixed with gRNA:Cas9 complexes and 3 µg ssDNA donor template (20 μL ...
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bioRxiv - Immunology 2024Quote: ... sgRNAs targeting Mavs and NLSx2-Cas9 protein were mixed with FLAMs in P2 primary cell nucleofection solution (Lonza, catalog no. V4XP-2024). Nucleofection was carried out using the 4D-Nucleofector® Core and X Unit (Lonza ...
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bioRxiv - Cell Biology 2024Quote: ... An amount of 106 cells were resuspended in 100 µl of SE Cell Line Solution (Cat. No. V4XC-2024, Lonza, Basel, Switzerland) containing 3.5 µg of the respective plasmid and electroprated with pulse DG113 ...
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bioRxiv - Genomics 2024Quote: ... Approximately 12 million cells per replicate were spun down and resuspended in nucleofection solution from the Lonza SF Cell Line 4D-Nucleofector X Kit L (Lonza, V4XC-2012) and nucleofected in 12 reactions with 5 μg pLJK07 or pLJK08 per 1 million cells according to manufacturer’s instructions ...
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bioRxiv - Immunology 2024Quote: ... One million NK cells were then resuspended in 20 µl of 4D Nucleofector Master Mix (82% P3 solution + 18% Supplement 1; Lonza, V4XP-3032) and mixed with the Cas9 RNP complex for electroporation using the CM137 program ...
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bioRxiv - Cancer Biology 2024Quote: ... 1 × 105 OVCAR8 landing pad cells were resuspended in 20 µl of nucleofector solution combined with supplement at 4.5:1 from the Amaxa SE Cell Line 4D-Nucleofector X Kit S (Lonza, Cat #V4XC-1032), before addition of 5 µl RNP complex and 1 µl of Electroporation enhancer ...
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bioRxiv - Developmental Biology 2024Quote: ... Transfection was performed by electroporation using an Amaxa Nucleofector II combined with Human Stem Cell Nucleofector Solution 2 (Lonza, ref #: VPH-5022) and programme B-16 ...
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bioRxiv - Cell Biology 2021Quote: ... The solution was transferred into a certified cuvette and cell electroporation was conducted using Nucleofector® program X-013 (Amaxa® Nucleofector®, Lonza). Electroporated cells were immediately resuspended in pre-warmed phenol-free Leibovitz’s-L-15 medium (Fisher ...
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bioRxiv - Neuroscience 2021Quote: ... 1 × 106 cells were resuspended in 100 μl of the nucleofection hESC solution 1 (Human Stem Cell Nucleofector® Kit 1/ Lonza #VPH-5012) where 5 μg of plasmids were added previously ...
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bioRxiv - Cancer Biology 2020Quote: ... A total of 1.5×106 tumor cells (td-tomato MDA-MB-231 SORE6>GFP) were transfected with 10 μl of 20 μM siRNA stock solution using Nucleofector Kit V from Lonza (cat# VCA-1003) for 48 h ...
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bioRxiv - Genomics 2021Quote: ... Growing CH12F3 cells were transfected with 1.5 μg pX330-Cas9 or pX330-Cas9-mCherry expression vector per million by 4D-nucleofector X (Lonza, solution M1, procedure DN100) and seeding at 0.5 million cells/mL in fresh medium with 1μg/mL anti-CD40 ...
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bioRxiv - Microbiology 2022Quote: ... Each pellet was carefully resuspended in 20 μL of SF Cell Line Nucleofector Solution with Supplement 1 (Lonza Cat. #V4XC-2032, Basel, Switzerland) and 1.5 μL of pX458 plasmids (150 ng each of BAX g2 ...
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bioRxiv - Molecular Biology 2022Quote: ... Cell pellets of 1E6 cells were resuspended with 20ul supplemented nucleofector solution of the P3 Primary Cell 4D-nucleofector® X Kit S (Lonza, V4XP-3032) and 2ug plasmid (1ug/ul) ...
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bioRxiv - Cell Biology 2023Quote: ... Next 1 × 106 cells resuspended in 93 μL of P3 Primary Cell Nucleofector Solution (P3 Primary Cell 4D-Nucleofector X Kit, Lonza, Catalog #: V4XP-3024) were mixed with 7 μL of RNP and 12 μg of single-stranded oligodeoxynucleotide (ssODN ...
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bioRxiv - Cancer Biology 2023Quote: ... The RNP complex was mixed with 8 x 10e5 GBM1 cells resuspended in 100 μl of SG solution from the SG Cell Line 4D-NucleofectorTM X Kit (Lonza, Cat# V4XC-3024). This entire mix was then placed in a NucleocuvetteTM and was nucleofected using a Lonza Amaxa 4D-Nucleofector TM and the EN138 nucleofection programme ...
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bioRxiv - Cancer Biology 2024Quote: ... 1 x 106 REH cells were transfected with 200 pmol of each siRNA pool (siCtrl, siH1-0_1, siH1-0_2) in the 100 µl nucleocuvette format using the 4D Nucleofector system (Lonza, SF solution, program DS-150).
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bioRxiv - Cell Biology 2024Quote: ... This mixture was incubated for 10 min-at room temperature and further diluted with 20 μL of P3 Primary Cell solution (Lonza, Walkersville, MD, USA). For the nucleofection process ...
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bioRxiv - Synthetic Biology 2024Quote: ... 1-5x105 iPSCs were resuspended in 20μL of P3 primary cell nucleofector solution plus supplement (cat.: V4XP-3032; Lonza Bioscience, Walkersville, MD, USA) along with the RNP complex and nucleofected using Lonza 4D Nucleofector (program CA-137) ...