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Citations for Constant Systems :
201 - 233 of 233 citations for T Cell Surface Glycoprotein CD3 Gamma Chain CD3G Antibody Biotin since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2024Quote: ... 2 mM β-mercaptoethanol) and disrupted by high pressure using a One Shot Cell Disruptor (Constant Systems) at 1.9 kbar ...
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Uptake of monoaromatic hydrocarbons during biodegradation by FadL channel-mediated lateral diffusionbioRxiv - Microbiology 2020Quote: ... pH 8) and ruptured by one passage at 23,000 psi through a cell disrupter (Constant Systems 0.75 kW). Total membranes were obtained by ultracentrifugation in a Beckman 45Ti rotor for 45 mins at 42,000 rpm at 4°C ...
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Uptake of monoaromatic hydrocarbons during biodegradation by FadL channel-mediated lateral diffusionbioRxiv - Microbiology 2020Quote: ... pH 8] and ruptured by one passage at 23,000 psi through a cell disrupter (Constant Systems 0.75 kW). Inclusion bodies and cell debris were collected by centrifugation at 17,000 × g for 20 mins ...
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bioRxiv - Biophysics 2023Quote: ... 5 mM ý-mercaptoethanol (bME)) using a French pressure cell disrupter (25 kpsi; Constant Systems Ltd, Daventry, UK), and the lysate was cleared by centrifugation at 20,000xg for 45 min at 4 °C ...
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bioRxiv - Microbiology 2024Quote: ... Lysis was performed using the One-shot cell disrupter at 1.5 Kbar (One shot model, Constant Systems Ltd). Lysates were centrifuged for 30 min at 30000 x g in 4 °C and the resulting supernatants were gently mixed with Ni-NTA Agarose beads (Qiagen ...
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bioRxiv - Plant Biology 2019Quote: ... 0.2% v/v TWEEN20 and EDTA-free protease inhibitor tablets and lysed using a cell disruptor (Constant Systems Ltd) at 14,000 psi ...
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bioRxiv - Microbiology 2022Quote: Bacterial cells were washed twice with protein purification buffer and disrupted by a French Press (Constant Systems Limited, UK). Cell debris was separated from the soluble fraction by centrifugation (20 min ...
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bioRxiv - Microbiology 2023Quote: ... Cells were harvested by centrifugation (6,000 g, 10 min, 4°C) and lysed using a French-press (Constant Systems) at 36,000 ps ...
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bioRxiv - Biochemistry 2019Quote: ... the pellet was dissolved in Buffer F (50 mM Tris/HCl, 200 mM NaCl, pH 7.5) and lysed by cell disruption at 35 kPSI (Constant Systems). The lysate was cleared by ultracentrifugation (2 h at 35,000 rpm in a 45 Ti rotor ...
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bioRxiv - Bioengineering 2020Quote: ... Cells were disrupted in a single pass through a bench top high pressure homogenizer (Constant Systems Ltd., Daventry, Northants, UK) operated at 10 kpsi ...
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bioRxiv - Plant Biology 2020Quote: ... 5 mM EDTA before being lysed at 2°C with an automatic cell pressure lysis machine at 0.3 KBar (Constant Systems) in homogenization buffer (50 mM Tris adjusted to pH 8.0 with MES ...
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bioRxiv - Plant Biology 2021Quote: ... in the presence of 5 mM EDTA and lysed by 1-2 passes through a cell disrupter operated at 35-37 kpsi (TS-Series 0.75 kW; Constant Systems). Membranes were collected from the suspension by centrifugation at 200,000 x g for 90 mins (45Ti rotor ...
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bioRxiv - Microbiology 2022Quote: ... Pellets were resuspended in TBS and lysed at a pressure of 23 Kpsi using a cell disrupter (Constant Systems 0.75kW). The membrane fraction was harvested by centrifugation (45 minutes at 42,000 r.p.m ...
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bioRxiv - Cell Biology 2023Quote: ... The cell pellets were resuspended in 50 mM sodium phosphate buffer (pH 7.4) supplemented with 300 mM NaCl and 10% glycerol and lysed by cell homogenization (Constant systems). Then the proteins were purified from the cell lysate through Ni2+-NTA affinity column (ÄKTA Pure ...
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bioRxiv - Biochemistry 2023Quote: ... 30 mM Imidazole and 0.02 mg/ml DNase I and broken with a Constant cell disruptor at 4 °C and 25 kPsi (Constant Systems). The cell lysate was clarified by centrifugation for 30 minutes at 30,000 x g ...
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bioRxiv - Molecular Biology 2021Quote: ... supplemented with 50 μg/mL DNase I and EDTA-free protease inhibitors) and lysed by passage through a cell disruptor at 24 kPSI (Constant Systems). Lysate was cleared by centrifugation (39,000 × g ...
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bioRxiv - Biochemistry 2020Quote: ... Cells were incubated on ice for 30 min before being lysed by two passages through a TS series 1.1kW cell disruptor (Constant System Ltd) at 30,000 PSI ...
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bioRxiv - Microbiology 2021Quote: ... The cell suspensions were incubated with DNase I (50 μg ml-1) and MgCl2 (2 mM) for 10 min at room temperature before extraction with a continuous-flow cell disruptor (Constant Systems) at 277 K and a pressure between 17-18 KPSI ...
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bioRxiv - Biochemistry 2022Quote: ... Cell pellets containing different subcomplexes were mixed in defined ratio (see below) and lysed at 4°C with a one-shot cell disruptor (Constant Systems). The lysate was clarified by centrifugation at 30 000 g for 30 min at 18°C ...
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bioRxiv - Microbiology 2022Quote: ... Thawed cell pellets were supplemented with an additional 1 mM PMSF and lysed using a One Shot homogeniser (Constant Systems Ltd) at 20 KPSI at 4°C and then centrifuged at 19 000 RPM in a JA-25.50 rotor (Beckman Coulter ...
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bioRxiv - Biochemistry 2023Quote: ... Cell pellets were resuspended in lysis buffer and lysed with a high pressure homogenizer (French press cell disruptor, CF1 series Constant Systems) at 4°C ...
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bioRxiv - Biochemistry 2022Quote: The thawed cell pellet was resuspended in 100 ml 1x phosphate-buffered saline (PBS) and passed through a continuous flow cell disruptor (Constant Systems) at 25 KPSI ...
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bioRxiv - Cell Biology 2023Quote: ... 20 mM imidazole) supplemented with protease inhibitor cocktail (cat. PIC002.1, BioShop) and lysed using a benchtop cell disruption unit (Constant Systems Ltd.). Affinity chromatography (HIS-Select™ Nickel Affinity Gel ...
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bioRxiv - Biochemistry 2023Quote: ... Cells were resuspended in membrane resuspension buffer (75mM Tris pH 8.0, 150 mM NaCl, and 5% glycerol) and then lysed using a cell disruptor (Constant Systems Ltd) at 28 ...
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bioRxiv - Biochemistry 2023Quote: ... Cell pellets were resuspended in lysis buffer and lysed with a high pressure homogenizer (French press cell disruptor, CF1 series Constant Systems) at 4°C ...
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bioRxiv - Microbiology 2023Quote: ... pH 7.6 sodium phosphate buffer (supplemented with 300 mM NaCl and DNase) and lysed using the high-pressure cell disrupter (Constant Systems), by running the sample twice under 1.9 kbar with subsequent cell fractionation using ultracentrifugation ...
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bioRxiv - Biochemistry 2023Quote: Cell pellets were resuspended in lysis buffer and lysed with a high pressure homogenizer (French press cell disruptor, CF1 series Constant Systems) at 4°C ...
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bioRxiv - Biochemistry 2024Quote: ... in a ratio of 7g/100 ml and lysed at 4 °C and 25 kPsi using a Cell Disruptor (Constant System). Cell debris was removed by centrifugation for 30 minutes at 13,000 × g ...
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bioRxiv - Microbiology 2020Quote: ... Bacterial pellets were resuspended in 1/50th of the original culture volume and lyzed using a cell disruptor (One shot model, Constant System Inc.). Proteins were purified by affinity chromatography using a GSTrap HP affinity column (GE Healthcare ...
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bioRxiv - Molecular Biology 2023Quote: ... cell pellets were lysed at 4°C using high-pressure homogenization at 25 Kpsi (1 Kpsi = 69 _ 103 MPa) (Constant System Ltd, UK) in lysis buffer [50 mM Tris-HCl pH:7.5 ...
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bioRxiv - Cell Biology 2022Quote: ... the pellet was resuspended in one volume of RP buffer and passed through a French press (One shot cell disruptor, Constant Systems LTD, Daventry, UK) at 2.2 Kpsi ...
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bioRxiv - Microbiology 2024Quote: ... The bacteria were harvested by centrifugation and lysed using a One-Shot cell disrupter following the manufacturer’s instructions (Constant Systems Ltd., Daventry, United Kingdom). Cell lysates were cleared by centrifugation ...
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bioRxiv - Cell Biology 2024Quote: ... the pellet was resuspended in one volume of RP buffer and passed through a French press (One shot cell disruptor, Constant Systems LTD, Daventry, UK) at 2.2 Kpsi ...