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Citations for Constant Systems :
101 - 150 of 247 citations for Cell Isolation Optimizing System since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2022Quote: ... Resuspended cells were lysed by using the cell disrupter (Constant systems, LTD, UK, TS-Series) and the soluble fraction containing the protein was separated from cell debris by centrifugation at 18,000 rpm for 30 min at 4°C ...
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bioRxiv - Physiology 2020Quote: ... and the cells were lysed by high-pressure cell disruption (Constant Systems Limited, United Kingdom). The His6-MBP-peptide fusion proteins were purified from the clarified soluble lysate over a nickel affinity resin ...
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bioRxiv - Microbiology 2021Quote: ... Cells were then lysed using a high-pressure homogenizer (Multi cycle cell disruptor, Constant Systems). Cell debris was removed by centrifugation at 15,000 × g for 20 minutes at 4°C ...
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bioRxiv - Biophysics 2021Quote: ... and the cells broken with a TS5/48/AE/6□A cell disrupter (Constant Systems) at 31,000□psi at 4°C ...
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bioRxiv - Cell Biology 2022Quote: ... Resuspended cells were lysed by using the cell disrupter (Constant systems, LTD, UK, TS-Series) and the soluble fraction containing the protein was separated from cell debris by centrifugation at 18,000 rpm for 30 min at 4 °C ...
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bioRxiv - Molecular Biology 2021Quote: ... Cells were disrupted by two passages at 207 MPa through a cell disruptor (Constant System). Large debris was removed by centrifugation for 15 min at 15,000 x g ...
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bioRxiv - Molecular Biology 2021Quote: ... Cells were lysed by two passages at 165 MPa in a cell disrupter (Constant System) and the debris separated from supernatant containing the soluble protein by centrifugation (30 min at 30,000 x g) ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... Cells were lysed at 30 kpsi using the One-Shot cell lyser (Constant Systems Ltd.) and upon addition of EDTA-free cOmplete™ protease inhibitor cocktail (Roche) ...
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bioRxiv - Biochemistry 2021Quote: ... Cells were broken with a cell disrupter with 4 cycles at 35 kPsi (Constant Systems) and cellular debris was removed by centrifugation at 90 000 x g for 20 min at 4°C (45 Ti rotor ...
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bioRxiv - Microbiology 2023Quote: ... cells were homogenized using a cell homogenizer at 30kpsi (One Shot Model; Constant Systems LTD).
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bioRxiv - Microbiology 2023Quote: ... Cell lysis was performed by 3 successive passages in a cell disrupter (Constant Systems Ltd) at 2.5 kbar pressure ...
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bioRxiv - Molecular Biology 2023Quote: ... cells were harvested by centrifugation and lysed by One shot cell disrupter (Constant Systems Ltd.) with a pressure of 2 kbar in lysis buffer (1 M NaCl ...
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bioRxiv - Molecular Biology 2024Quote: ... The cells were disrupted at 20 kPsi (one shot) in Cell Disruptor (Constant Systems Ltd.) and centrifuged (20000 × g ...
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bioRxiv - Biochemistry 2020Quote: ... then lysed using a cell disruptor (Constant Systems Ltd., United Kingdom). The lysate was cleared by centrifugation (23,500 × g ...
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bioRxiv - Microbiology 2020Quote: ... DNase-treated and homogenised using a cell disruptor (Constant Systems Ltd). The lysate was centrifuged (35,000 x g ...
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bioRxiv - Microbiology 2021Quote: ... Cells were disrupted twice using a One-Shot disrupter (Constant Systems). The lysate was then centrifuged for 15 minutes at 20,000 rcf ...
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bioRxiv - Plant Biology 2020Quote: ... The cells were then disrupted under French press (Constant systems limited) in a lysis phosphate buffered saline (PBS ...
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bioRxiv - Microbiology 2022Quote: ... passed through a One-Shot cell disruptor (Constant systems, Northants, UK) at 30 kPsi ...
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bioRxiv - Biochemistry 2021Quote: ... Dissolved bacteria were lysed using a cell disrupter (Constant Systems LTD.). Cell debris was centrifuged 2 h at 50’000 x g ...
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bioRxiv - Biochemistry 2022Quote: ... Both were then lysed in a cell disruptor (Constant Systems Ltd.) and the resulting samples were clarified by centrifugation in an SS34 rotor (Sorvall ...
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bioRxiv - Microbiology 2022Quote: ... and lysed in a cell disrupter (Constant Systems 0.75 kW model) at 20-23,000 psi (1 pass ...
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bioRxiv - Biophysics 2024Quote: ... 10% glycerol) using a continuous flow cell disruptor (Constant Systems Ltd.). Lysate was centrifuged at 15.000xg for 30 min ...
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bioRxiv - Biochemistry 2019Quote: ... Cells were lysed using a cell disruptor in one-shot mode (Constant Systems, Daventry, United Kingdom) and lysates were clarified by spinning for 30 min at 16,000 RPM in a Sorvall SS-34 rotor at 4°C ...
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bioRxiv - Biochemistry 2019Quote: ... Cells were lysed using a cell disruptor in one-shot mode (Constant Systems, Daventry, United Kingdom) and lysates were clarified by spinning for 30 min at 16,000 RPM in a Sorvall SS-34 rotor at 4°C ...
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bioRxiv - Biochemistry 2019Quote: ... Cells were lysed using a cell disruptor in one-shot mode (Constant Systems, Daventry, United Kingdom) and lysates were clarified by spinning for 30 min at 16,000 RPM in a Sorvall SS-34 rotor at 4°C ...
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bioRxiv - Biochemistry 2019Quote: ... Cells were lysed using a cell disruptor in one-shot mode (Constant Systems, Daventry, United Kingdom) and lysates were clarified by spinning for 30 min at 16,000 RPM in a Sorvall SS-34 rotor at 4°C ...
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bioRxiv - Biochemistry 2021Quote: ... Cells were harvested 2-3 hours later and lysed using a cell disrupter (Constant Systems Ltd.). Proteins were purified from inclusion bodies using Nickel affinity chromatography on prepacked HisTrap FF columns (Cytiva ...
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bioRxiv - Biochemistry 2023Quote: Resuspended cells were further lysed by using the cell disrupter (Constant systems, LTD, UK, TS- Series) and the soluble fraction containing GST-Mint1 was separated from cell debris by centrifugation at 18,000 rpm for 30 min at 4 οC ...
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bioRxiv - Microbiology 2021Quote: ... Cells in this resuspension were lysed by two passes through a One-Shot cell disruptor (Constant Systems) at 23 kpsi and the cell debris was removed by centrifugation (40,000 x g at 4°C for 30 min) ...
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bioRxiv - Biochemistry 2021Quote: ... Cells were lyzed using a cell disruptor with pressure of 1.8 kbar (Constant Systems Ltd., Northants UK). After centrifugation (24 000 x g for 30 min at 4 °C) ...
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bioRxiv - Biochemistry 2021Quote: ... The cell disruption was performed by the cell homogenizer at 15000 psi (Constant Systems Ltd, United Kingdom). Cell lysate was centrifuged at 50000 g ...
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bioRxiv - Microbiology 2022Quote: ... The cells were lysed with a single pass at 25 kpsi through a cell disruptor (Constant Systems). The lysates were supplemented with 1 mM PMSF ...
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bioRxiv - Biophysics 2022Quote: ... Cells were lysed by homogenization in a fluidizer (EmulsiFlex-C3, Avestin or CF1 cell disrupter, Constant Systems) using 3 passes ...
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bioRxiv - Cell Biology 2021Quote: ... Cells were passed one time through a French press (Constant Systems LTD) at 4°C with 2.2 kpsi of pressure ...
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bioRxiv - Microbiology 2022Quote: ... The cells were disrupted using a micro-fluidizer (Constant system LTD, UK) at 14,000 Psi ...
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bioRxiv - Microbiology 2022Quote: ... and lysed by two passages through a cell disruptor (Constant systems Ltd.) at 25,000 psi ...
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bioRxiv - Biochemistry 2022Quote: ... The sample was then passed through a cell disruptor (Constant Systems Ltd.) for lysis (2 passes at 25 kPSI) ...
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bioRxiv - Microbiology 2022Quote: ... and lysed twice using a One Shot cell disrupter (Constant Systems, Inc.) at 27 kpsi and 4 °C ...
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bioRxiv - Biochemistry 2022Quote: ... and were broken by two passages through a cell disruptor (Constant Systems) at 18 000 psi ...
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bioRxiv - Neuroscience 2022Quote: ... Cell pellets were collected and subjected to high pressure lysis (Constant System) in lysis buffer (50 mMTris pH 7.5 ...
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bioRxiv - Cell Biology 2022Quote: ... 500 mM KCl and lysed using a TS Cell Disruptor (Constant Systems) at 172 MPa ...
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bioRxiv - Biochemistry 2023Quote: ... and lysed by a CF2 cell disruptor (Constant Systems Ltd., Daventry, UK). The lysate was centrifuged for one hour at 13,000 ×g at 4 °C ...
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bioRxiv - Cell Biology 2022Quote: ... cracked in a cell disruptor (Constant Systems; 2 cycles at 25 kpsi), and clarified by centrifugation (45 min ...
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bioRxiv - Biochemistry 2023Quote: ... and lysed at 35,000 psi using a CF1 Cell Disruptor (Constant Systems). The lysate was cleared by centrifugation at 20,000 rpm for 30 min at 4 °C then syringe filtered using a 0.45 μm filter ...
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bioRxiv - Immunology 2023Quote: ... Bacteria were harvested by centrifugation and lysed using Cell Disruptor (Constant System) at 1.3 kbar ...
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bioRxiv - Molecular Biology 2023Quote: ... Cells were lysed by a French press (Constant Systems Ltd, Daventry, England). After centrifugation ...
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bioRxiv - Biophysics 2024Quote: ... 0.1 mM PMSF) using a continuous flow cell disruptor (Constant Systems Ltd.). The lysate was centrifuged at 20.000xg for 60 min and the supernatant was further incubated with Ni-NTA beads for 2 h at 4°C ...
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bioRxiv - Biochemistry 2024Quote: ... 5mM β-mercaptoethanol) and homogenized utilizing One Shot Cell Disruptor (Constant Systems) operating at 1.0 kbar ...
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bioRxiv - Molecular Biology 2023Quote: ... the cells were lysed by French press (Constant Systems Ltd, Daventry, England), centrifuged at 10,000 rpm for 1 h ...
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bioRxiv - Cell Biology 2021Quote: Harvested cells were lysed by high-pressure homogenization using a continuous flow cell disruptor (Constant Systems Limited, UK) at 32 kPsi at 5°C with addition of 0.5% w/v Triton X-100 followed by 30 min high-speed centrifugation at 38,000 × g using Beckman JA 25.5 rotor ...