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Citations for Sanquin :
1 - 50 of 56 citations for Rat T Cell Surface Glycoprotein CD3 Gamma Chain CD3G ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2019Quote: Human T cells were cultured in T-cell mixed media (Miltenyi) supplemented with 5% heat-inactivated human serum (Sanquin) and fetal bovine serum (FBS ...
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bioRxiv - Immunology 2020Quote: ... T cell culture medium consisted of 5% human serum (Sanquin), 5% FCS ...
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bioRxiv - Immunology 2022Quote: ... Antigen-specific T cells were detected using PE-conjugated s598 tetramers (Sanquin Reagents) after an incubation of 20 min at 37 °C ...
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bioRxiv - Immunology 2022Quote: ... Cells were expanded using agonistic mAbs against CD3 (clone CLB-T3/4.E, IgE isotype, Sanquin, 0.1 μg/ml) and CD28 (clone CLB-CD28/1 ...
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bioRxiv - Immunology 2023Quote: Cytokines in cell-free supernatants were measured using enzyme-linked immunosorbent assay (ELISA) for IL-6 (Sanquin), TNF-α (Diaclone ...
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bioRxiv - Immunology 2022Quote: ... and stimulated with plate-bound anti-CD3 (16A9, 1 µg/mL, Sanquin) and soluble anti-CD28 (15E8 ...
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bioRxiv - Immunology 2020Quote: ... CD4+CD45RA+ T cells were activated for 5-6 days with 1.5 µg/ml plate bound αCD3 (Sanquin) and 1 µg/ml soluble αCD28 (Sanquin ...
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bioRxiv - Immunology 2020Quote: ... IFN-γ or GM-CSF release in the cell culture supernatant was measured using ELISA according to the manufacturer’s protocol (Sanquin and BioLegend, respectively).
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bioRxiv - Immunology 2020Quote: ... Cytokine content of cell free supernatants was determined using standard sandwich ELISA according to the manufacturer’s instructions (IFN-γ and GM-CSF; Sanquin and Biolegend). Some target cells were pre-incubated for 2 days in the presence or absence of UGCG inhibitors.
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bioRxiv - Cancer Biology 2022Quote: ... 2-3 wells containing 0.5-1 x106 live cells from the tissue digest were cultured for 10-13 days in 24 wells plates in 20/80 T-cell mixed media (Miltenyi) containing 5% human serum (HS) (Sanquin), 5% FBS ...
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bioRxiv - Immunology 2019Quote: ... 2-3 wells containing 0,5-1*106 live cells from the tissue digest were cultured for 10-13 days in 24 wells plates in 20/80 T-cell mixed media (Miltenyi) containing 5% human serum (HS) (Sanquin), 5% FBS ...
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bioRxiv - Immunology 2024Quote: 1-3×106 cells were cultured in 24-well plates at 37°C and 5% CO2 in 20/80 T-cell mixed media (Miltenyi) containing 5% human serum (HS) (Sanquin), 5% FBS ...
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bioRxiv - Immunology 2020Quote: ... and incubated with culture supernatants (diluted in high-performance ELISA buffer, Sanquin Reagents). Plates were again washed five times and incubated for one hour with horseradish peroxidase-conjugated mouse-anti-human-IgG (1 µg/ ml ...
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bioRxiv - Cancer Biology 2023Quote: ... PE-conjugated streptavidin was diluted in high performance ELISA buffer (HPE) (Sanquin, Amsterdam, The Netherlands). The Biorad FlexMAP3D (Biorad laboratories ...
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bioRxiv - Cancer Biology 2023Quote: ... PE-conjugated streptavidin was diluted in high performance ELISA buffer (HPE) (Sanquin, Amsterdam, The Netherlands). The Biorad FlexMAP3D (Biorad laboratories ...
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bioRxiv - Immunology 2023Quote: ... Purified CD4+ T cells were seeded at 0.5*10^6 cells/ml in plates coated with 0.5 μg/ml anti-CD3 (CLB-T3/4.E, 1XE) and 70 ng/ml soluble anti-CD28 (CLB-CD28/1, 15E8; both from Sanquin). Cells were cultured in a total volume of 1 ml consisting of medium only ...
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bioRxiv - Immunology 2023Quote: ... were stimulated as described [6] in flat-bottom 96 well plates with a soluble anti-CD3 IgE subclass (T3/4.E, Sanquin, Amsterdam ...
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bioRxiv - Immunology 2024Quote: ... In a regular 96 ELISA flat bottom plate 15% normal human serum (Sanquin, Amsterdam, The Netherlands) was added to the liposome mixture (R1 from the kit ...
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The transcription factor RUNX2 drives the generation of human NK cells and promotes tissue residencybioRxiv - Immunology 2022Quote: ... after which supernatant was collected and analysed with IFN-γ ELISA assay (PeliKine-Tool Set, Sanquin, Amsterdam, The Netherlands).
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bioRxiv - Immunology 2019Quote: ... were pre-coated overnight at 4°C with 4μg/mL rat α-mouse IgG2a (MW1483, Sanquin) in PBS ...
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bioRxiv - Bioengineering 2019Quote: ... and a subsequent incubation with phycoerythrin-conjugated streptavidin (10 min) which was diluted in high performance ELISA (HPE) buffer (Sanquin). Data acquisition was executed using a FLEXMAP 3D system controlled with xPONENT 4.1 software (Luminex ...
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bioRxiv - Bioengineering 2020Quote: ... the supernatants were consecutively incubated with antibody-conjugated MagPlex microspheres (1 h), biotinylated antibodies (1 h), and streptavidin-phycoerythrin (10 min, diluted in high performance ELISA (HPE) buffer (Sanquin)) ...
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bioRxiv - Neuroscience 2021Quote: ... Plates were washed and incubated at room temperature for 30 minutes with horseradish peroxidase-conjugated streptavidin (strep-HRP; 1:10000 Streptavidin Poly HRP, Sanquin, Amsterdam, dilution in High Performance ELISA Buffer, HPE, Sanquin, Amsterdam). Plates were washed and 100 ul of substrate solution ...
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bioRxiv - Immunology 2022Quote: ... followed by 1 h incubation with biotinylated antibodies and 10 min incubation with phycoerythrin-conjugated streptavidin in high performance ELISA buffer (HPE, Sanquin, Hambug, Germany). Data acquisition was performed with FLEXMAP 3D equipment in combination with xPONENT software (version 4.3 ...
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bioRxiv - Bioengineering 2022Quote: ... followed by 1 h incubation with biotinylated antibodies and 10 min incubation with phycoerythrin-conjugated streptavidin in high performance ELISA buffer (HPE, Sanquin, Hamburg, Germany). Data acquisition was performed with FLEXMAP 3D equipment in combination with xPONENT software (version 4.3 ...
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bioRxiv - Immunology 2019Quote: ... 24-well plates were pre-coated overnight with 0.5 μg/mL rat α-mouse IgG2a (clone MW1483, Sanquin) at 4 °C ...
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bioRxiv - Immunology 2022Quote: ... plates were washed five times with PBS-T and incubated with 1 µg/ml anti-human IgG-horseradish-peroxidase (HRP) (clone: MH16.1, Sanquin, Amsterdam, the Netherlands). After washing ...
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bioRxiv - Immunology 2023Quote: ... Treg cells (105 cells/well) were added and the medium was supplemented with soluble anti-CD3ε [25] (T3/4.E, Sanquin). T3/4.E is able to stimulate Tresp cells directly in soluble form [25] ...
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bioRxiv - Cancer Biology 2021Quote: ... Peripheral blood mononuclear cells (PBMCs) were obtained from Sanquin Blood bank (Amsterdam ...
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bioRxiv - Immunology 2022Quote: ... PB-NK cells were obtained from buffy coats of healthy donors (Sanquin) after written informed consent according to the Declaration of Helsinki and all experimental protocols concur to institutional guidelines ...
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bioRxiv - Immunology 2022Quote: ... The cells were thawed in medium with 71% Human Serum (HS; Sanquin), 0.03% DNAse and 0.1%MgCL2 and were washed (at 300 g for 15 minutes ...
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bioRxiv - Bioengineering 2020Quote: ... Peripheral blood mononuclear cells (PBMCs) were isolated from blood of healthy donors (Sanquin) using standard Ficoll (GE Healthcare ...
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bioRxiv - Microbiology 2021Quote: ... and 3-5% human blood type O red blood cells (RBCs) (Sanquin, the Netherlands) at 37°C in 3% O2 ...
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bioRxiv - Bioengineering 2022Quote: Peripheral blood mononuclear cells (PBMCs) were isolated from human peripheral blood buffy coats (Sanquin, Eindhoven ...
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The tumour microenvironment shapes dendritic cell plasticity in a human organotypic melanoma culturebioRxiv - Immunology 2019Quote: Peripheral blood mononuclear cells (PBMCs) were isolated from buffy coats obtained from healthy volunteers (Sanquin) and purified via centrifugation over a Ficoll density gradient (Axis-Shield ...
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bioRxiv - Immunology 2020Quote: Peripheral blood mononuclear cells (PBMCs) were obtained from whole blood of healthy donors (from Sanquin Institute Amsterdam or from GSK Stevenage Blood Donation Unit ...
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bioRxiv - Cancer Biology 2020Quote: ... Peripheral blood mononuclear cells (PBMCs) were isolated from healthy donor buffy coats (Sanquin, Amsterdam, the Netherlands) by density gradient centrifugation using Lymphoprep (Stem cell technologies ...
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bioRxiv - Cancer Biology 2024Quote: Peripheral blood mononuclear cells (PBMCs) were isolated from buffy coats obtained from healthy blood donors (Sanquin). Written informed consent for research use of donated blood was obtained by the Sanquin blood bank ...
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bioRxiv - Cell Biology 2019Quote: Dendritic cells were derived from peripheral blood monocytes isolated from a buffy coat (Sanquin, Nijmegen, The Netherlands) (de Vries et al. ...
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bioRxiv - Cell Biology 2023Quote: Dendritic cells were derived from peripheral blood monocytes isolated from a buffy coat (Sanquin, Nijmegen, The Netherlands) (de Vries et al ...
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bioRxiv - Immunology 2023Quote: Dendritic cells were derived from peripheral blood monocytes isolated from a buffy coat (Sanquin, Nijmegen, The Netherlands) 37 ...
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bioRxiv - Cell Biology 2023Quote: Peripheral blood mononuclear cells (PMBCs) were isolated from buffy coats obtained from healthy donors (Sanquin, The Netherlands) by density gradient centrifugation with Ficoll-Paque PLUS (GE Healthcare) ...
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bioRxiv - Immunology 2024Quote: ... Human healthy donor peripheral blood mononuclear cells (PBMC) were isolated from buffy coats (Sanquin, Amsterdam, the Netherlands) using Ficoll-Paque according to the manufactureŕs protocol ...
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bioRxiv - Immunology 2023Quote: Human cDC2s were isolated from peripheral blood mononuclear cells derived from healthy individuals (Sanquin, Nijmegen, the Netherlands) using the MACS CD1c+ isolation kit (130-119-475 ...
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bioRxiv - Genomics 2021Quote: ... Peripheral blood mononuclear cells (PBMCs) were isolated from buffy coats from healthy volunteers (Sanquin Bloodbank, Nijmegen, the Netherlands). Isolation was performed using Ficoll-Paque ...
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bioRxiv - Bioengineering 2022Quote: Peripheral blood mononuclear cells (PBMCs) were isolated from a human peripheral blood buffy coat (Sanquin, Eindhoven, The Netherlands) of one healthy donor after written informed consent per the Declaration of Helsinki and according to the institutional guidelines ...
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bioRxiv - Microbiology 2021Quote: ... Fresh human serum and human red blood cells (RBC) were obtained from the Dutch National Blood Bank (Sanquin Amsterdam ...
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bioRxiv - Cell Biology 2023Quote: Peripheral blood mononuclear cells (PBMCs) were isolated from human peripheral blood buffy coats of two healthy donors (Sanquin, Eindhoven ...
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bioRxiv - Bioengineering 2019Quote: Human peripheral blood mononuclear cells (hPBMCs) were isolated from two different buffy coats of healthy donors (purchased from Sanquin Blood Supply Foundation ...
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bioRxiv - Immunology 2019Quote: Peripheral mononuclear blood cells (PBMC) were isolated from peripheral blood or buffy coats of healthy individuals collected by Sanquin Blood Supply (Amsterdam ...