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Citations for Sanquin :
51 - 80 of 80 citations for HLA A*0201 NY ESO 1 complex Protein Human HEK293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2021Quote: Monocytes were seeded at a density of 100 000 cells per well in 96-well plates and were maturated for six days in Roswel Park Memorial Institute-1640 (RPMI-1640) medium containing 10% pooled human serum (Sanquin), 1% (v/v ...
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bioRxiv - Immunology 2019Quote: ... peripheral blood and uterine Tregs and Tconv were washed in PBS and resuspended in basic medium with 10% human AB serum (Sanquin). Previously isolated and frozen healthy donor (HC ...
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bioRxiv - Immunology 2020Quote: ... CD8+ T cell clones recognizing peptides derived from the endogenously expressed proteins USP11 and SSR1(7, 8) were expanded using a standard feeder mix in IMDM supplemented with 5% human serum (Sanquin) and 5% FCS(9).
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bioRxiv - Immunology 2019Quote: ... 2-3 wells containing 0,5-1*106 live cells from the tissue digest were cultured for 10-13 days in 24 wells plates in 20/80 T-cell mixed media (Miltenyi) containing 5% human serum (HS) (Sanquin), 5% FBS ...
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bioRxiv - Microbiology 2023Quote: Human blood samples were isolated from buffy coats obtained from healthy donors after written informed consent (Sanquin, Amsterdam, the Netherlands). The biological samples were sourced ethically and their research use was in accordance with the terms of the informed consents under an IRB/EC approved protocol ...
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bioRxiv - Cancer Biology 2024Quote: Monocytes were seeded at a density of 1 x 10 cells per well in 96-well plates and incubated to mature for 6 days in RPMI-1640 medium containing 10% pooled human serum (Sanquin), 1% (v/v ...
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bioRxiv - Immunology 2024Quote: 1-3×106 cells were cultured in 24-well plates at 37°C and 5% CO2 in 20/80 T-cell mixed media (Miltenyi) containing 5% human serum (HS) (Sanquin), 5% FBS ...
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bioRxiv - Cell Biology 2021Quote: ... GFP-tagged human vWF plasmid (Romani de Wit et al., 2003) was kindly provided by Jan Voorberg (Stichting Sanquin Bloedvoorziening, Netherland) under Materials Transfer Agreement ...
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bioRxiv - Evolutionary Biology 2020Quote: ... aegypti Liverpool strain mosquitoes (BEI Resources, NR-48921) were starved for 24 h and subsequently fed with human blood (Sanquin Blood Supply Foundation) using a Hemotek membrane feeding system ...
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bioRxiv - Immunology 2021Quote: ... diluted in PBS for 10 min at RT and Fc-receptors were subsequently blocked using 5% human serum (HS; Sanquin Blood bank, Amsterdam, The Netherlands) for 45 min at RT ...
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bioRxiv - Immunology 2020Quote: ... and 1 µg/ml soluble αCD28 (CLB-CD28/1, 15E8 Sanquin) in the presence or absence of milk EVs or EV depleted control ...
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bioRxiv - Immunology 2020Quote: ... and cultured with 70 ng/ml – 1 µg/ml soluble αCD28 (CLB-CD28/1, 15E8 from Sanquin), RPMI 1640 medium (Gibco ...
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bioRxiv - Cell Biology 2022Quote: ... CD63/Lamp3 (EM 1:20, #M1544, Sanquin), claudin 3 (IF 1:200 ...
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bioRxiv - Immunology 2020Quote: ... and 1 µg/ml soluble αCD28 (Sanquin) in medium ...
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bioRxiv - Bioengineering 2020Quote: ... the supernatants were consecutively incubated with antibody-conjugated MagPlex microspheres (1 h), biotinylated antibodies (1 h), and streptavidin-phycoerythrin (10 min, diluted in high performance ELISA (HPE) buffer (Sanquin)) ...
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bioRxiv - Immunology 2020Quote: ... and IgG-DyLight 650 (clone MH16-1, Sanquin Reagents ...
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bioRxiv - Immunology 2020Quote: ... were coated overnight with anti-IgG1 or anti-IgG4 (2 µg/ml clones MH161-1, MH161-1, MH164-4 respectively, Sanquin Reagents) in PBS ...
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bioRxiv - Cancer Biology 2021Quote: ... Granzyme B-PE (1:200, clone GB11, Sanquin Amsterdam). To detect cytokine production ...
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bioRxiv - Immunology 2022Quote: ... and CD28 (clone CLB-CD28/1, Sanquin, 0.2 μg/ml) in solution ...
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bioRxiv - Immunology 2022Quote: ... and soluble anti-CD28 (15E8, 1 µg/mL, Sanquin (10)) ...
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bioRxiv - Immunology 2022Quote: ... and stimulated with plate-bound anti-CD3 (16A9, 1 µg/mL, Sanquin) and soluble anti-CD28 (15E8 ...
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bioRxiv - Immunology 2024Quote: ... Anti-CD103-BV711 (Ber-ACT8) and anti-Hobit-Alexa647 (Sanquin-Hobit/1) were purchased from BD Biosciences ...
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bioRxiv - Immunology 2022Quote: ... and anti-IgG (clone G18-145, BD Bioscience; clone MH16-1, Sanquin Reagents). Samples were measured on LSRII and analyzed using Flowjo software (Treestar).
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bioRxiv - Cell Biology 2020Quote: ... Sections were blocked for 30 min with 1% bovine serum albumin (BSA; Sanquin, The Netherlands) in tris-buffered saline (TBS) ...
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bioRxiv - Immunology 2019Quote: ... Platelets were re-pelleted and resuspended in 1 mL SSP+ (Storage solution for platelets, Sanquin, Amsterdam, the Netherlands). Platelet count and platelet activation were determined by flow cytometry (FACS Calibur ...
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bioRxiv - Immunology 2021Quote: ... biotinylated recombinant antibodies that were able to bind the antigen were detected with 1/10,000 poly-HRP-conjugated streptavidin (Sanquin) diluted in PTA buffer (100 μL/well ...
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bioRxiv - Neuroscience 2021Quote: ... Plates were washed and incubated at room temperature for 30 minutes with horseradish peroxidase-conjugated streptavidin (strep-HRP; 1:10000 Streptavidin Poly HRP, Sanquin, Amsterdam ...
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bioRxiv - Immunology 2023Quote: ... Purified CD4+ T cells were seeded at 0.5*10^6 cells/ml in plates coated with 0.5 μg/ml anti-CD3 (CLB-T3/4.E, 1XE) and 70 ng/ml soluble anti-CD28 (CLB-CD28/1, 15E8; both from Sanquin). Cells were cultured in a total volume of 1 ml consisting of medium only ...
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bioRxiv - Neuroscience 2021Quote: ... Plates were washed and incubated at room temperature for 30 minutes with horseradish peroxidase-conjugated streptavidin (strep-HRP; 1:10000 Streptavidin Poly HRP, Sanquin, Amsterdam, dilution in High Performance ELISA Buffer, HPE, Sanquin, Amsterdam). Plates were washed and 100 ul of substrate solution ...
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bioRxiv - Immunology 2020Quote: ... FcγRIII were immunoprecipitated from the neutrophil cell lysate using a mouse anti-CD16 monoclonal IgG2a antibody (Ref M9087, Clone CLB-FcR gran/1, 5D2, Sanquin, Amsterdam, The Netherlands). Prior to usage ...