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Citations for Sanquin :
51 - 81 of 81 citations for Human Nectin 4 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2023Quote: Human cDC2s were isolated from peripheral blood mononuclear cells derived from healthy individuals (Sanquin, Nijmegen, the Netherlands) using the MACS CD1c+ isolation kit (130-119-475 ...
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bioRxiv - Immunology 2024Quote: ... Human healthy donor peripheral blood mononuclear cells (PBMC) were isolated from buffy coats (Sanquin, Amsterdam, the Netherlands) using Ficoll-Paque according to the manufactureŕs protocol ...
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bioRxiv - Cancer Biology 2021Quote: ... 2011) where TEGs were cultured in ‘TEG culture medium’ (RPMI-Glutamax supplemented with 2,5 – 10 % human serum (Sanquin), 1% pen/strep and 0.5M beta-2-mercaptoethanol ...
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bioRxiv - Bioengineering 2022Quote: Peripheral blood mononuclear cells (PBMCs) were isolated from a human peripheral blood buffy coat (Sanquin, Eindhoven, The Netherlands) of one healthy donor after written informed consent per the Declaration of Helsinki and according to the institutional guidelines ...
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bioRxiv - Cell Biology 2023Quote: Peripheral blood mononuclear cells (PBMCs) were isolated from human peripheral blood buffy coats of two healthy donors (Sanquin, Eindhoven ...
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bioRxiv - Bioengineering 2019Quote: Human peripheral blood mononuclear cells (hPBMCs) were isolated from two different buffy coats of healthy donors (purchased from Sanquin Blood Supply Foundation ...
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bioRxiv - Microbiology 2020Quote: ... Fresh human serum and red blood cells (RBC) for these experiments were obtained from the Dutch National Blood Bank (Sanquin Amsterdam ...
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bioRxiv - Cancer Biology 2022Quote: ... 2-3 wells containing 0.5-1 x106 live cells from the tissue digest were cultured for 10-13 days in 24 wells plates in 20/80 T-cell mixed media (Miltenyi) containing 5% human serum (HS) (Sanquin), 5% FBS ...
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bioRxiv - Immunology 2021Quote: Monocytes were seeded at a density of 100 000 cells per well in 96-well plates and were maturated for six days in Roswel Park Memorial Institute-1640 (RPMI-1640) medium containing 10% pooled human serum (Sanquin), 1% (v/v ...
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bioRxiv - Immunology 2019Quote: ... peripheral blood and uterine Tregs and Tconv were washed in PBS and resuspended in basic medium with 10% human AB serum (Sanquin). Previously isolated and frozen healthy donor (HC ...
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bioRxiv - Immunology 2020Quote: ... CD8+ T cell clones recognizing peptides derived from the endogenously expressed proteins USP11 and SSR1(7, 8) were expanded using a standard feeder mix in IMDM supplemented with 5% human serum (Sanquin) and 5% FCS(9).
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bioRxiv - Immunology 2019Quote: ... 2-3 wells containing 0,5-1*106 live cells from the tissue digest were cultured for 10-13 days in 24 wells plates in 20/80 T-cell mixed media (Miltenyi) containing 5% human serum (HS) (Sanquin), 5% FBS ...
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bioRxiv - Immunology 2020Quote: ... Anti-RBD and anti-NP IgG antibodies were measured in EDTA plasma samples at 100-1200 fold dilutions using ELISA as previously described.11 Plates were coated with RBD or N protein and specific IgG antibodies were detected using anti-human IgG (MH16, Sanquin).
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bioRxiv - Microbiology 2023Quote: Human blood samples were isolated from buffy coats obtained from healthy donors after written informed consent (Sanquin, Amsterdam, the Netherlands). The biological samples were sourced ethically and their research use was in accordance with the terms of the informed consents under an IRB/EC approved protocol ...
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bioRxiv - Cancer Biology 2024Quote: Monocytes were seeded at a density of 1 x 10 cells per well in 96-well plates and incubated to mature for 6 days in RPMI-1640 medium containing 10% pooled human serum (Sanquin), 1% (v/v ...
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bioRxiv - Immunology 2024Quote: 1-3×106 cells were cultured in 24-well plates at 37°C and 5% CO2 in 20/80 T-cell mixed media (Miltenyi) containing 5% human serum (HS) (Sanquin), 5% FBS ...
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bioRxiv - Immunology 2020Quote: ... coated with 1.5 µg/ml αCD3 (CLB-T3/4.E, 1XE from Sanquin, Amsterdam, The Netherlands) and cultured in RPMI 1640 medium (Gibco ...
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bioRxiv - Immunology 2019Quote: ... were pre-coated overnight at 4°C with 4μg/mL rat α-mouse IgG2a (MW1483, Sanquin) in PBS ...
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bioRxiv - Cell Biology 2021Quote: ... GFP-tagged human vWF plasmid (Romani de Wit et al., 2003) was kindly provided by Jan Voorberg (Stichting Sanquin Bloedvoorziening, Netherland) under Materials Transfer Agreement ...
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bioRxiv - Immunology 2020Quote: ... Plates were again washed five times and incubated for one hour with horseradish peroxidase-conjugated mouse-anti-human-IgG (1 µg/ ml, clone MH16-1, Sanquin Reagents). After a final five-times wash ...
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bioRxiv - Immunology 2020Quote: ... coated with 0.5 – 1.5 µg/ml αCD3 (CLB-T3/4.E, 1XE from Sanquin, Amsterdam, The Netherlands) and cultured with 70 ng/ml – 1 µg/ml soluble αCD28 (CLB-CD28/1 ...
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bioRxiv - Immunology 2022Quote: ... plates were washed five times with PBS-T and incubated with 1 µg/ml anti-human IgG-horseradish-peroxidase (HRP) (clone: MH16.1, Sanquin, Amsterdam, the Netherlands). After washing ...
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bioRxiv - Evolutionary Biology 2020Quote: ... aegypti Liverpool strain mosquitoes (BEI Resources, NR-48921) were starved for 24 h and subsequently fed with human blood (Sanquin Blood Supply Foundation) using a Hemotek membrane feeding system ...
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bioRxiv - Immunology 2022Quote: ... Cells were expanded using agonistic mAbs against CD3 (clone CLB-T3/4.E, IgE isotype, Sanquin, 0.1 μg/ml) and CD28 (clone CLB-CD28/1 ...
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bioRxiv - Immunology 2020Quote: 10×106 cells/ml CD4+CD45RA+ T cells were cultured for 3 days in the presence of 1.5 µg/ml plate bound αCD3 (CLB-T3/4.E, 1XE Sanquin) and 1 µg/ml soluble αCD28 (CLB-CD28/1 ...
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bioRxiv - Immunology 2021Quote: ... diluted in PBS for 10 min at RT and Fc-receptors were subsequently blocked using 5% human serum (HS; Sanquin Blood bank, Amsterdam, The Netherlands) for 45 min at RT ...
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bioRxiv - Genomics 2021Quote: ... 10 ml of blood was obtained from 9 consenting anonymous healthy blood donors (HBDs; 5 females and 4 males) via the Dutch National blood bank (Sanquin) in CellSave tubes ...
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bioRxiv - Immunology 2023Quote: ... Purified CD4+ T cells were seeded at 0.5*10^6 cells/ml in plates coated with 0.5 μg/ml anti-CD3 (CLB-T3/4.E, 1XE) and 70 ng/ml soluble anti-CD28 (CLB-CD28/1, 15E8; both from Sanquin). Cells were cultured in a total volume of 1 ml consisting of medium only ...
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bioRxiv - Immunology 2023Quote: ... Treg cells (105 cells/well) were added and the medium was supplemented with soluble anti-CD3ε [25] (T3/4.E, Sanquin). T3/4.E is able to stimulate Tresp cells directly in soluble form [25] ...
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bioRxiv - Immunology 2023Quote: ... were stimulated as described [6] in flat-bottom 96 well plates with a soluble anti-CD3 IgE subclass (T3/4.E, Sanquin, Amsterdam ...
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bioRxiv - Immunology 2020Quote: ... were coated overnight with anti-IgG1 or anti-IgG4 (2 µg/ml clones MH161-1, MH161-1, MH164-4 respectively, Sanquin Reagents) in PBS ...