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Citations for Active Motif :
451 - 500 of 508 citations for WD Repeat Containing Planar Cell Polarity Effector WDPCP Antibody Biotin since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2020Quote: ... Fibroblast cell nuclear fraction was extracted using a commercially available kit (Active motif, 40010) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2021Quote: Chromatin immunoprecipitation was performed using the Low Cell ChIP-Seq kit (#53048, Active Motif) following the manufacturer’s instructions ...
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bioRxiv - Pathology 2023Quote: ... Cells were lysed to ChIP-IT Express Magnetic Chromatin Immunoprecipitation kit (Active Motif 53008). Optimal sonication conditions were determined previously in order to obtain DNA fragments of about 500 bp ...
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bioRxiv - Microbiology 2023Quote: ... Cells were lysed using 100 µl of ice-cold ATAC lysis buffer (Active Motif), followed immediately by addition of 1 ml pre-chilled wash buffer (10 mM Tris-HCl pH 7.4 ...
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bioRxiv - Developmental Biology 2022Quote: ... Antibody incubation (DUX: sc-137190; Santa Cruz Biotechnology, Santa Cruz, CA, USA; SIRT1: 8469S; CST, Danvers, MA, USA; H3K27ac: 39034; Active Motif, Carlsbad, CA, USA) was performed overnight at 4°C ...
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bioRxiv - Genomics 2020Quote: ... were raised against KLH-conjugated dinucleosides at the Technology Development Laboratory (Babraham Bioscience Technologies Ltd, Cambridge, UK) (anti-mCA antibody clone 2C8H8A6 currently available from Active Motif, Cat. No 61783/4). Alexa Fluor secondary antibodies were purchased from Life Technologies ...
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bioRxiv - Genomics 2020Quote: ... the RevMAb Biosciences (clone RM362, at 1:300 dilution) and the mouse monoclonal recombinant AbFlex m6A antibody (rAb) from Active Motif (at 1:300 dilution). A mixture of reference DNA oligonucleotides that correspond to the position of the modified nucleotides was used to compare with antibody binding (the last base at the 5’ end of the oligonucleotide hybridizes with the modified base ...
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bioRxiv - Developmental Biology 2020Quote: ... pre-cleared for 2 h followed by immunoprecipitation with primary antibodies for 12-16 hours at 4°C (H3K27ac, Active motif 39133; H3K27me3, CST 9733s). 5% of samples were used as input DNA ...
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bioRxiv - Molecular Biology 2022Quote: ... After an overnight incubation at 4°C with the primary antibodies (anti-5mC, Eurogentec, ref BI-MECY-0100, 1/500; anti-5hmC, Active Motif, ref 39569, 1/500), and a 1-hour incubation at room temperature with secondary antibodies (Dylight 488 anti-mouse ...
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bioRxiv - Biochemistry 2021Quote: ... The cells were then washed in 1x PBS twice followed by histone extraction (Active Motif). The extracted histones were then western blotted for H2B-Ub levels (Cell Signaling ...
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bioRxiv - Cancer Biology 2020Quote: Nuclear extracts from Jurkat cells were prepared using a Nuclear Extract Kit (40010, Active Motif), according to manufacturer’s recommendation ...
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bioRxiv - Genomics 2022Quote: ... 0.5 million G1-phase DLD-1 cells were processed according to manufacturer’s instructions (Active Motif). Samples were paired-end sequenced to obtain at least 107 reads ...
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bioRxiv - Developmental Biology 2023Quote: ... Cells were incubated overnight at 4oC with mouse anti-PLZF (0.2 μg/ml, Active Motif) primary antibody ...
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bioRxiv - Genomics 2023Quote: ... chromatin was extracted from CT26 cells using the Nucleosome Preparation Kit (cat: 53504, Active Motif). The enzymatic digestion time for this cell line was optimized to 30min and the mononucleosome preparation protocol was followed as per manufacturer’s recommendations ...
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bioRxiv - Systems Biology 2019Quote: ... Permeabilized cells were incubated overnight at 4°C with 5ug of anti-H3K27me3 (Active Motif 39156) and then washed before incubating with protein A-MNase fusion protein (a gift from S ...
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bioRxiv - Microbiology 2021Quote: Nuclear proteins were isolated from challenged A549 cells using a nuclear protein extraction kit (Active Motif) according to manufacturer’s specifications and protein concentration was determined using a bicinchoninic acid (BCA ...
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bioRxiv - Cell Biology 2021Quote: ... cells were re-suspended in Glycine Stop-Fix Solution (ChIP-IT Enzymatic Express Kit; Active Motif) and kept shaking for 5 mins at room temperature ...
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bioRxiv - Physiology 2019Quote: ... Ishikawa cell nuclear and cytoplasmic protein fractions were extracted using Nuclear Extraction Kit (Active Motif, Belgium) according to manufacturer’s instructions ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: Nuclear proteins were isolated from RAW264 cells using Nuclear Extract Kit (Active Motif, Carlsbad, CA, USA) according to the manufacturer’s protocols ...
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bioRxiv - Cancer Biology 2023Quote: ... and cytosolic extracts from NES cells were prepared by using the Nuclear Extract Kit (Active Motif) as per the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2023Quote: ATACseq was used to evaluate the chromatin of MG63 cells (Active Motif, Carlsbad, CA, USA; 53150). At the Sylvester Comprehensive Cancer Center’s Oncogenomic Core Facility ...
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bioRxiv - Cell Biology 2020Quote: Histones were purified from cells by growing macrophages to a density of 3×107 (Active Motif - 40025). Histone H3K27me3 conversion was measured using the Active Motif kit (Epiquik Histone Methyltransferase activity assay kit (P-3005).
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bioRxiv - Cancer Biology 2021Quote: Chromatin was isolated from 4T1 and 6DT1 cells using the ChIP-IT Express Enzymatic kit (Active Motif) according to manufacturers’s instructions as mentioned above ...
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bioRxiv - Molecular Biology 2021Quote: ... the cells were washed with 0.1 % PBST three times and blocked in MAXblock Blocking Medium (Active Motif) for 1 hour at room temperature ...
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bioRxiv - Molecular Biology 2022Quote: ... chromatin from 1 x 107 cells were mixed with 20 ng spike-in chromatin (Active Motif, #53083) and incubated with 4-8 μg primary target antibody and 2 μg spike-in antibody (Active Motif ...
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bioRxiv - Microbiology 2023Quote: ... Histones were isolated from inhibitor treated or untreated cells using the histone extraction kit (Active Motif, 40028) and western blots performed ...
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bioRxiv - Genomics 2024Quote: ... and Illumina sequencing libraires were prepared using the Low Cell ChIP-Seq kit (Active Motif, Carlsbad, CA) according to the manufacturer instructions ...
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bioRxiv - Cancer Biology 2021Quote: ... fractionation of MDA-MB-231 and 4T1 cells was first performed using the Nuclear Extract kit (Active Motif). The level of protein binding to DNA consensus sequence was then assessed by ELISA using the TransAM NF-kB Family kit (Active Motif ...
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bioRxiv - Cancer Biology 2024Quote: ... Fixed cells were washed with chilled PBS-Igepal (0.5% Igepal CA-630 in PBS) and PMSF (Active Motif). Washed cells were centrifuged ...
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bioRxiv - Cancer Biology 2024Quote: ... Cell pellets used for fractionation were fractionated using the Nuclear Complex Co-IP kit from Active motif (#54001) according to their protocol.
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bioRxiv - Developmental Biology 2019Quote: ... 5 µl was transformed into chemically competent TAM-1 cells in a 96-well plate format (Active Motif #11096) and selected for Chloramphenicol resistance ...
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bioRxiv - Genomics 2021Quote: ... Rabbit anti-H3K4me3 (1:100 for bulk profiling or 1:10 for single-cell experiments, Active Motif Cat# 39159), Rabbit anti-H3K36me3 (1:100 for bulk profiling or 1:10 for single-cell experimemts ...
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bioRxiv - Cancer Biology 2021Quote: Cells from two independent experiments were collected and open chromatin was assessed using an ATACseq kit (Active Motif, 53150). DNAseq library preparation was completed at the Oncogenomics Core Facility at the Sylvester Comprehensive Cancer Center ...
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bioRxiv - Cancer Biology 2021Quote: ... cells were harvested and nuclear protein complex lysates were prepared using the Nuclear Complex Co-IP Kit (Active Motif). Co-immunoprecipitation with two biological replicates was performed according to the manufacturer’s instructions ...
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bioRxiv - Physiology 2020Quote: ... Cells were then washed twice more with ice cold PBS before homoengisation with a Dounce homogenizer (Active Motif, 40415) in Fast IP buffer (150mM NaCl ...
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bioRxiv - Genomics 2022Quote: ... aliquots of ∼500,000 cells were added to 20 µl Dynabeads Protein A with 2 µg anti-H3K27ac (Active Motif) and incubated with slow rotation at 4°C overnight ...
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bioRxiv - Cancer Biology 2021Quote: ... MM.1S cells were exposed to Dex or EtOH for 1 hour and fixed according to the manufacturer’s instructions (RIME Cell Fixation protocol, Active Motif). Analysis were performed by Active motif and resuslts are given as supplementary table (see Additional file 2 ...
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bioRxiv - Immunology 2020Quote: ... Nuclear extract from HuT78 cells (endogenous SP140) was prepared following manufacturer’s protocol (Active Motif nuclear extract and co-IP kit). Protein concentration was determined by Bradford Assay (Pierce) ...
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bioRxiv - Cell Biology 2020Quote: ... Sandwich ELISA to quantify phosphorylated IκBα in infected and non-infected cells at 0-120 minutes after infection (Function ELISA IκBα, S32/S36, Active Motif). iii ...
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bioRxiv - Cancer Biology 2023Quote: ChIP assays at the HES1 promoter were performed in HT29 cells using the CHIP-IT kit (Active Motif, Carlsbad, USA). Sonicated chromatin was immunoprecipitated with antibodies against IgG (sc-3739 ...
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bioRxiv - Immunology 2024Quote: ... and total RNA fractions were prepared from A549 cells according to the RNA Subcellular Isolation Kit (Active Motif, Cat. #25501). cDNA was prepared as described above and qPCR was performed to analyze VILMIR expression in both cellular fractions ...
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bioRxiv - Immunology 2021Quote: ... Antibody of interest was added to sonicated chromatin aliquot and mixed (anti-H3K27ac Cell Signaling, Catalog # 8173, 5μl; anti-H3K27me3 Active Motif, Catalog # 39155 ...
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bioRxiv - Plant Biology 2021Quote: ... 30 ml cell suspension were sonicated for 5 min (5s on/10s off, 60% amplitude) with a sonicator (EpiShear, Active Motif) equipped with a ¼” microtip probe ...
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bioRxiv - Cancer Biology 2022Quote: ... Nuclear extraction of MDA-MB-231 cells was done according to manufactureŕs instructions (Universal Magnetic Co-IP Kit, Active Motif, 54002). Imaging flow cytometry analysis was conducted on an Amnis Imagestream Mk (>100,000 events ...
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bioRxiv - Genetics 2020Quote: ... Chr13.1-cl and Chr13.2-cl KO ES cells was determined by native ChIP-seq and anti-H3K9me3 serum (Active Motif, #39161) as described previously (45) ...
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bioRxiv - Molecular Biology 2024Quote: ATAC-seq libraries were generated from liver sinusoidal endothelial (LSEC) and liver-resident immune cryopreserved cells using the ATAC-seq kit (Active Motif). H3K27ac histone modification data was generated from liver sinusoidal endothelial (LSEC ...
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bioRxiv - Cancer Biology 2024Quote: ... GSCs (10 million cells / Co-IP) were collected and prepared for nuclear extraction and purification using a nuclear extraction kit (Active Motif). Nuclear extracts were treated with an enzymatic shearing Cocktail for DNA digestion to release undissociated protein complexes ...
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bioRxiv - Synthetic Biology 2021Quote: ... half of each original cell aliquot was incubated with Dynabeads protein-G conjugated with 5 µg of rabbit polyclonal to Histone H3K27me3 (Active motif 39155) or rabbit polyclonal to Histone H3 (acetyl K27 ...
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bioRxiv - Neuroscience 2021Quote: The rest of the ChIP experiment (beginning from “Protein G Agarose Bead Preparation”) was carried out using the reagents and protocols from the Low Cell ChIP-Seq Kit (Active Motif, 53084). In brief ...
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bioRxiv - Molecular Biology 2023Quote: ... Antibodies against target histone modifications were used to detect respective bands (Anti H3K27Me3 #9733 Cell Signaling, Anti H3K9Me #ABE101 Merck Millipore, Anti H3K56Ac #61061 Active Motif, Anti H3K9Ac #39038 Active Motif ...