Labshake search
Citations for Active Motif :
551 - 600 of 683 citations for SpectraDye Antibody Labeling Kit 490 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Cancer Biology 2019Quote: Cytoplasmic and nuclear RNA were isolated and purified using the RNA Subcellular Isolation Kit (Active Motif) following the manufacturer’s protocol ...
-
bioRxiv - Physiology 2019Quote: ... Ishikawa cell nuclear and cytoplasmic protein fractions were extracted using Nuclear Extraction Kit (Active Motif, Belgium) according to manufacturer’s instructions ...
-
bioRxiv - Cancer Biology 2019Quote: ... Quality of ChIP-enriched DNA was assessed using the ChIP-IT qPCR Analysis Kit (Active Motif).
-
bioRxiv - Genomics 2020Quote: ChIP for small cell numbers was performed with a Low Cell ChIP-Seq Kit (Active Motif) according to the manufacturer’s manual (version A3 ...
-
bioRxiv - Developmental Biology 2020Quote: ... then incubated 3 min with Stop Solution from the ChIP-IT High Sensitivity Kit (Active Motif) and washed three times with PBS before being frozen at −80°C until further use ...
-
bioRxiv - Pharmacology and Toxicology 2020Quote: Nuclear proteins were isolated from RAW264 cells using Nuclear Extract Kit (Active Motif, Carlsbad, CA, USA) according to the manufacturer’s protocols ...
-
bioRxiv - Pathology 2022Quote: Co-immunoprecipitation (Co-IP) was conducted using the Nuclear Complex Co-IP Kit (Active Motif, 54001) according to the manufacturer’s manual ...
-
bioRxiv - Molecular Biology 2023Quote: ... Unmethylated cytosines were converted into uracil by using a MethylDetector kit (55001, Active Motif, CA, USA), whereas methylated cytosines remained unchanged ...
-
bioRxiv - Molecular Biology 2023Quote: ATAC-seq libraries were prepared using ATAC-seq kit from Active Motif (Active Motif, CA, # 53150) according to the manual and sequenced on the Illumina NovaSeq 6000 Sequencer (2×150 ...
-
bioRxiv - Cancer Biology 2023Quote: ... and cytosolic extracts from NES cells were prepared by using the Nuclear Extract Kit (Active Motif) as per the manufacturer’s instructions ...
-
bioRxiv - Molecular Biology 2023Quote: Co-immunoprecipitation (Co-IP) was performed using the Nuclear Complex Co-IP kit (Active Motif, USA) and Dynabeads™ Protein G for Immunoprecipitation according to manufacturer’s protocol (Invitrogen ...
-
bioRxiv - Molecular Biology 2023Quote: ... Tagmentation and indexing was performed according to the instructions of the ATAC-seq Kit (Active Motif). Library quality control was performed on a Bioanalyzer and sequenced at the Genomics Core Facility (GCF ...
-
bioRxiv - Molecular Biology 2024Quote: ... Luciferase activity was measured 24 hours post-transfection using a LightSwitch Luciferase Assay kit (Active Motif) on Spectramax iD3 multi-mode microplate reader (Molecular Devices ...
-
bioRxiv - Cancer Biology 2024Quote: ... Global DNA methylation was studied using the global DNA methylation assay kit - LINE-1 (Active Motif) according to the manufacturer’s instructions.
-
bioRxiv - Cancer Biology 2021Quote: ... Relative 5hmC levels were quantified blotting 500 ng of gDNA on a nitrocellulose membrane with an anti-5hmC antibody (Active Motif, 39769) diluted 1/10000 followed by an anti-rabbit antibody coupled to horseradish peroxydase (Dutscher NA934 ...
-
bioRxiv - Genomics 2020Quote: ... and incubated with the appropriate primary antibody for 1 h at room temperature (anti-RNAPII: 1:500, 61086, Active Motif; anti-H3K27ac: 1:500, 39133, Active Motif; anti-H3K27me3 ...
-
bioRxiv - Microbiology 2022Quote: ... Membranes were blocked in 5% milk in PBS-T for 1 h at room temperature and incubated overnight at 4°C with primary antibodies against Lamin AC (Active Motif 39287), Lamin B1 (Abcam ab16048) ...
-
bioRxiv - Genomics 2019Quote: ... We performed western blots against both GAPDH (GAPDH antibody (6C5) Life Technologies AM4300) and the Carboxyl Terminal Domain of Pol-II (CTD) (Pol II CTD Ser5-P antibody, Active Motif, 61085). We ran each lysate on Mini-protean TGX precast gels (bioRad 456-1093 ...
-
bioRxiv - Molecular Biology 2019Quote: ... and coupled to one of the following antibodies in the same buffer for 1 hr at room temperature with rotation: 3 μl H3 (Active motif 39763), 5 μl H3K4me3 (Millipore 04-745) ...
-
Targeted attenuation of elevated histone marks at SNCA alleviates α-synuclein in Parkinson’s diseasebioRxiv - Neuroscience 2020Quote: ... the following primary antibodies were used: (Normal mouse IgG, Millipore, 12-371; H3K4me3, Abcam, ab8580; H3K27me3, Active motif, 39155; H3K27ac, Abcam, ab4729). Anti-Cas9 antibody (Takara ...
-
bioRxiv - Developmental Biology 2020Quote: ... Beads were then resuspended in Blocking buffer and coated with antibodies for 4 h at 4°C (H4K20me1 [0.5 μg/IP], Active Motif cat. 39727). Once coated beads were magnet-separated and resuspended in 1 mL of lysate ...
-
bioRxiv - Molecular Biology 2022Quote: ... Cell fractionations were verified by western blot as described in 57 with antibodies against RNA polymerase II CTD phospho Ser2 (Active Motif, 61984) and GAPDH (GeneTex GT239 or ThermoFisher MA5-15738) ...
-
bioRxiv - Developmental Biology 2023Quote: ... and chromatin was incubated for 2h at 4°C with 5 μg of anti-H3K27ac antibody (Active Motif, Cat# 39133, Lot 01613007). Immuno-complexes were sequentially washed ...
-
bioRxiv - Microbiology 2023Quote: ... Beads were resuspended in 100 μl of IP/wash buffer and coated with 5 μg of rabbit anti-m6A antibody (Active motif, 61495) for 45 min at room temperature with rotation ...
-
bioRxiv - Molecular Biology 2024Quote: ... Primary antibodies (anti-BRD4, Cell Signaling Technologies Mouse mAb #63759 at 1:1000 dilution, anti-H3K27ac, Active Motif #39133, 1:1000 dilution) were prepared in blocking buffer and incubated on the sample overnight at 4 °C ...
-
bioRxiv - Cell Biology 2024Quote: ... The H3K27Ac ChIP reactions were conducted with 10 μg of tissue chromatin and 4 μg of H3K27Ac antibody (Active Motif, cat # 39133). The H3K4me1 ChIP reactions were carried out by using 10 ug of tissue chromatin and 4 ul of H3K4me1 antibody (Active Motif ...
-
bioRxiv - Cancer Biology 2021Quote: The amount of GTP-bound RAS was determined using the Ras GTPase Chemi ELISA Kit (Active Motif North America ...
-
bioRxiv - Molecular Biology 2021Quote: Nuclear proteins were prepared using fibroblasts at 80-90% confluency and the Nuclear Extract Kit (Active Motif), according to the manufacturer’s protocol.
-
bioRxiv - Genomics 2019Quote: Extraction of nuclear proteins and coimmunopreciptation were performed using the Universal Magnetic Co-IP Kit (Active Motif). Briefly ...
-
bioRxiv - Developmental Biology 2019Quote: ... followed by 3 minutes incubation with Stop Solution from the ChIP-IT High sensitive kit (Active Motif). Samples were then washed 3 times with working Washing Solution (ChIP-IT ...
-
bioRxiv - Cancer Biology 2021Quote: Chromatin immunoprecipitation (ChIP) was carried out using the ChIP-IT Express Enzymatic Chromatin Immunoprecipitation Kit (Active Motif) according to manufacturer’s instructions ...
-
bioRxiv - Cancer Biology 2021Quote: Chromatin was isolated from 4T1 and 6DT1 cells using the ChIP-IT Express Enzymatic kit (Active Motif) according to manufacturers’s instructions as mentioned above ...
-
bioRxiv - Molecular Biology 2020Quote: ... ChIP-seq libraries were constructed using the Next Gen DNA Library Kit (Active Motif 53216 and 53264). Library quality was assessed using Agilent 2100 Bioanalyzer and Agilent High Sensitivity DNA assay ...
-
bioRxiv - Neuroscience 2022Quote: CUT&Tag was performed from 450k nuclei using the Cut&TagIT Kit following manufacturer’s protocol (Active Motif).
-
bioRxiv - Immunology 2019Quote: ... Subsequent chromatin preparation steps were performed according to the “High Sensitivity Chromatin Preparation” Kit (Active Motif, 53046). Samples were sonicated two times for 10 minutes (30 seconds on ...
-
bioRxiv - Developmental Biology 2021Quote: ChIP experiments were carried out using the ChIP-IT Express Enzymatic kit (Cat. No. 53009, Active Motif) or the True MicroChIP Kit (Cat ...
-
bioRxiv - Developmental Biology 2021Quote: ... The supernatant was removed and the beads washed with 665µL of ChIP Buffer (from Active Motif kit) by inverting the tube several times ...
-
bioRxiv - Molecular Biology 2021Quote: ATAC-seq was performed in biological duplicate using the ATAC-Seq Kit (Active Motif, catalog No.53150). Briefly ...
-
bioRxiv - Neuroscience 2022Quote: For DNA Chromatin Immunoprecipitation (ChIP) we used the ChIP-IT High Sensitivity® kit from Active motif. Lysates were obtained as described in the RIP protocol ...
-
bioRxiv - Cell Biology 2023Quote: ... and nuclei was extracted using ice-cold ATAC-lysis buffer (according to the kit protocol; Active Motif) and the pellet resuspended in the Tn5 tagmentation master mix ...
-
bioRxiv - Molecular Biology 2023Quote: ... ChIP-seq libraries were constructed using the Next Gen DNA Library Kit (Active Motif, 53216 and 53264). Library quality was assessed using Agilent 2100 Bioanalyzer and Agilent High Sensitivity DNA assay ...
-
bioRxiv - Microbiology 2023Quote: ... Histones were isolated from inhibitor treated or untreated cells using the histone extraction kit (Active Motif, 40028) and western blots performed ...
-
bioRxiv - Genomics 2024Quote: ... and Illumina sequencing libraires were prepared using the Low Cell ChIP-Seq kit (Active Motif, Carlsbad, CA) according to the manufacturer instructions ...
-
bioRxiv - Cell Biology 2023Quote: ... ChIP was then performed using a ChIP-IT High Sensitivity kit (#53040, Active Motif, Carlsbad, CA, USA) according to the manufacturer’s instructions ...
-
bioRxiv - Cell Biology 2020Quote: ... Primary antibodies (DSHB: Pax7: PAX7, eMyHC: F1.652, MHC:20-s, Abcam: Tfr1: ab84036 and Active Motif: MyoD:39991; Ki67:ZM-0167, ZSGB-Bio) were incubated overnight at 4 °C and secondary antibodies at RT for 1 hr at dark ...
-
bioRxiv - Molecular Biology 2022Quote: ... The following antibodies and dilutions were used: anti-Esrrb (PP-H6705-00, Perseus Proteomics, 1:1000) and anti-Histone H3 (61476, Active Motif, 1:5000).
-
bioRxiv - Genetics 2022Quote: ... Around 20 μg or 10 μg of chromatin were incubated overnight at 4°C with 10 μg H3.X/Y or 2.5 μg H3K9me3 antibody respectively (Active Motif-61161, Abcam-ab8898). Chromatinantibody extracted with incubated with protein G Dynal beads (Thermo Fisher Scientific ...
-
bioRxiv - Molecular Biology 2023Quote: ... All incubations with primary antibodies were performed for either 1h at room temperature or overnight at 4°C with the following antibodies for H3K9me3 (Active Motif, 1:1000), DAXX (Santa Cruz ...
-
bioRxiv - Molecular Biology 2023Quote: ... and immunoprecipitation performed over night at 4 °C on a spinning wheel by adding 10 µl of the respective antibody (Anti H3K27Me3 #9733 Cell Signaling, Anti H3 #2650 Cell Signaling, Anti H3K56Ac #61061 Active Motif). Afterwards ...
-
bioRxiv - Developmental Biology 2023Quote: ... Human induced pluripotent stem cell derived cardiomyocytes (iPSC-CMs) from four HLHS patients were fixed in 4% paraformaldehyde and stained with antibody against 5-methylcytosine (Active Motif, RRID: AB_2687950)31 ...