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Citations for Active Motif :
51 - 100 of 100 citations for Siglec 10 Mouse HEK293 Fc since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genomics 2019Quote: ... The primary antibodies used were mouse anti-CAS9 (7A9-3A3, Active Motif), and mouse anti-GAPDH (sc-51907 ...
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bioRxiv - Genomics 2019Quote: ... 5µl of rabbit anti-mouse IgG secondary antibody (Active Motif cat.53017) were added and incubated for an additional 2 h at 4 °C with agitation ...
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bioRxiv - Developmental Biology 2023Quote: ... mouse anti-MEIS1/2/3 (0.5 µg/ml, 39795, Active Motif, RRID:AB_2750570), rabbit anti-DHRS3 (1:500 ...
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bioRxiv - Molecular Biology 2023Quote: ... or 10 μL anti-RNA pol II (Active Motif 39097) conjugated to 50 μL Dynabeads Protein G overnight at 4 °C ...
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bioRxiv - Neuroscience 2019Quote: ... at 1:5000 and mouse monoclonal anti-5-methylcytosine antibody (Active Motif, 39649) at 1:2000 ...
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bioRxiv - Genomics 2022Quote: ... secondary antibody conjugates from the TAM-ChIP Rabbit and Mouse kits (Active Motif) were used.
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bioRxiv - Immunology 2022Quote: ... or 10 μL of H3K27Ac antibody (Active Motif #39133, lot: 16119013). Total volume of bead plus antibody was brought to 200 μL using binding buffer and were rotated at room temperature for 30 min ...
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bioRxiv - Cell Biology 2019Quote: ... Antibodies were used at the following dilutions: anti-HIRA mouse monoclonal (WC119, Active Motif) IF 1:200 and Western Blot (WB ...
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bioRxiv - Cell Biology 2021Quote: ... mouse or rabbit anti-H3K27ac (1:250; 39085, Active Motif; 1:1000; ab4729, Abcam), rabbit anti-Lamin B1 (1:100 ...
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bioRxiv - Genomics 2019Quote: ... Membranes were incubated in anti-5-hydroxymethylcytidine (Active Motif, mouse monoclonal, 59.1, 0.4ug/mL) for 3hr at RT in 5% non-fat dry milk in PBS ...
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bioRxiv - Genomics 2023Quote: ... Antibodies used: mouse anti-Srebp1 (39939, Active Motif, Carlsbad, CA, USA, dilution 1:1,000), rabbit anti-Srebp2 (112046 ...
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bioRxiv - Neuroscience 2019Quote: ... rabbit polyclonal anti-histone H2AX phosphor Ser139 (Active Motif, AR-0149-10) at 1:200 ...
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bioRxiv - Neuroscience 2019Quote: ... rabbit polyclonal anti-histone H2AX phosphor Ser139 (Active Motif, AR-0149-10) at 1:200 ...
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bioRxiv - Developmental Biology 2019Quote: ... The primary antibodies used were as follows: H3K27me3 (mouse, 1:500, Active motif, cat. 61017), H3K27ac (rabbit ...
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bioRxiv - Genetics 2019Quote: ... and mouse monoclonal anti-H3K27me3 antibody (Active Motif MABI0323, Lot 17019020; 2 µL per IP). Immunocomplex was captured by Protein A-conjugated sepharose beads (for rabbit antibodies ...
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bioRxiv - Developmental Biology 2023Quote: ... Cells were incubated overnight at 4oC with mouse anti-PLZF (0.2 μg/ml, Active Motif) primary antibody ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... we incubated them with HRP-conjugated goat anti-mouse IgG (0.5 μg/ml, Active Motif, USA) at 25 ◦C for 1 h ...
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bioRxiv - Bioengineering 2023Quote: ... and mouse anti-MEIS 1/2/3 antibody (1:200, clone 9.2.7, 39796, Active Motif, RRID:AB_2750570). Secondary antibodies (raised in donkey ...
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bioRxiv - Cancer Biology 2020Quote: ... RRID:AB_2716801) for Ptch1+/-;BcorΔE9-10 and Ptch1+/- tumor samples was performed by Active Motif. One sample of Ptch1+/-;BcorΔE9-10 was discarded for outlier levels of Igf2 revealed by RNA-seq after ChIP analysis ...
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bioRxiv - Developmental Biology 2020Quote: ... 50 μL beads with 10 μg of the following antibodies – H3K4Me3 (Active Motif 28431), H3K27Me3 (Merck Millipore 07-449) ...
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bioRxiv - Cell Biology 2023Quote: ... 100 µg of chromatin supplemented with 10 ng of Spike-in chromatin (Active Motif) were used per ChIP experiment ...
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bioRxiv - Genomics 2022Quote: ... at a concentration of 10 ng/million cells and spike-in antibody (Active Motif, 61686) at a concentration of 0.4 µg/million cells were added following lysis and sonication but prior to immunoprecipitation ...
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bioRxiv - Immunology 2024Quote: ... ChIP-validated anti-mouse Ikaros antibody (cat #39355) and H3K27Ac antibody (cat #39133) were purchased from Active Motif. For Foxp3 ChIP-seq ...
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bioRxiv - Molecular Biology 2020Quote: ... or 10 μl anti- H3K56ac for the early-S time point (Active Motif, 39281 (lot# 14013003). Immunoprecipitation ...
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bioRxiv - Neuroscience 2023Quote: ... or anti-Mouse AbberiorSTAR440 1:200 (Abberior, Göttingen, Germany) and anti-Rabbit Chromeo505 1:200 (Active Motif, Carlsbad, USA)) in 1X Roti®-ImmunoBlock in dark condition at room temperature for 1 hr ...
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bioRxiv - Developmental Biology 2022Quote: ... This DNA was then used in immunoprecipitation (IP) reactions using 5-Methylcytosine (5-mC) mouse monoclonal antibody (39649, Active Motif). Immunoprecipitated DNA and input control (pooled DNA that did not go through the IP step ...
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bioRxiv - Molecular Biology 2020Quote: ... 4 - 10 µg of target antibodies and 2μg of spike-in antibody (anti-H2Av, Active Motif, #61686) were added and incubated on a rotator at 4°C overnight ...
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bioRxiv - Pathology 2023Quote: ... ChIP reactions were supplemented with 10 ng of Drosophila melanogaster chromatin (spike in chromatin, Active motif, 08221011), and 1 μg of an antibody recognizing H2Av ...
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bioRxiv - Cancer Biology 2020Quote: ... cells were blocked using LI-COR blocking buffer for 1 hour followed by overnight incubation with 1:50 mouse H4K20me3 (39672, Active Motif) at 4°C ...
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bioRxiv - Neuroscience 2021Quote: ... The sonicated lysate was added to 20 µl Dynabeads Protein A with 1.5 ug anti-H3K27ac (Active Motif, #39685 Mouse Monoclonal) and incubated with slow rotation at 4°C overnight ...
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bioRxiv - Cancer Biology 2020Quote: ... and 10 mM glycerophosphate) with the addition of 40 ng Spike-in Chromatin per reaction (Active Motif 53083). Immunoprecipitation was conducted using 4 µg of anti-H3 (Abcam ab1791) ...
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bioRxiv - Cancer Biology 2023Quote: Reactions (10 μL) containing 150 nM of PRC2 (comprising EZH2, SUZ12, EED and RbAp46/48; Active Motif, 31887), 1 µM recombinant nucleosomes wrapped with 187bp DNA (EpiCypher 16-2004) ...
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Targeted attenuation of elevated histone marks at SNCA alleviates α-synuclein in Parkinson’s diseasebioRxiv - Neuroscience 2020Quote: ... the following primary antibodies were used: (Normal mouse IgG, Millipore, 12-371; H3K4me3, Abcam, ab8580; H3K27me3, Active motif, 39155; H3K27ac, Abcam, ab4729). Anti-Cas9 antibody (Takara ...
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bioRxiv - Molecular Biology 2024Quote: ... Primary antibodies (anti-BRD4, Cell Signaling Technologies Mouse mAb #63759 at 1:1000 dilution, anti-H3K27ac, Active Motif #39133, 1:1000 dilution) were prepared in blocking buffer and incubated on the sample overnight at 4 °C ...
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bioRxiv - Genomics 2021Quote: ... Rabbit anti-H3K4me3 (1:100 for bulk profiling or 1:10 for single-cell experiments, Active Motif Cat# 39159), Rabbit anti-H3K36me3 (1:100 for bulk profiling or 1:10 for single-cell experimemts ...
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bioRxiv - Cell Biology 2021Quote: ... while negative control was incubated with mouse IgG and input DNA without antibody using the CHIP—IT Express HT Kit from Active Motif (Cat no: 53018) according to the manufacturer’s instructions [Supplementary Table III] ...
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bioRxiv - Biochemistry 2021Quote: ... rabbit polyclonal anti-Pre6 (gift from D. Wolf, 1:5000) and anti-Rpb1 (RNA Pol II) monoclonal mouse antibody (Active Motif, 4H8, 1:2000). For rabbit primary antibodies ...
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bioRxiv - Cell Biology 2021Quote: ... while negative control was incubated with mouse IgG and input DNA without antibody using the CHIP—IT Express HT Kit from Active Motif (Cat no: 53018) according to the manufacturer’s instructions [Supplementary Table III] ...
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bioRxiv - Genetics 2024Quote: CUT&Tag analysis of NaBu treated CCD841 cells and mouse intestinal samples were performed using CUT&Tag-IT™ assay kits (Active Motif # 53160, 53170). Approximately 30 mg of tissue was homogenized per reaction by first chopping frozen tissue into smaller pieces in a petri dish with a razor blade on dry ice ...
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bioRxiv - Genetics 2021Quote: ... Coverslips were removed and slides were washed in PBS with 0.1% Triton X-100 (PBST) (3X 10 minutes each) and subsequently incubated with primary rabbit anti-H3K9me3 (Active Motif #39765) antibodies (at 1:500 dilution ...
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bioRxiv - Plant Biology 2021Quote: ... 30 ml cell suspension were sonicated for 5 min (5s on/10s off, 60% amplitude) with a sonicator (EpiShear, Active Motif) equipped with a ¼” microtip probe ...
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bioRxiv - Neuroscience 2022Quote: ... ChIP reactions were carried out overnight at 4 °C with 3 µg of sheared chromatin and 10 μg of anti-H3K27me3 rabbit polyclonal antibody (39155, Active Motif) or anti-mouse IgG as negative control (115-001-003 ...
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bioRxiv - Cancer Biology 2024Quote: ... GSCs (10 million cells / Co-IP) were collected and prepared for nuclear extraction and purification using a nuclear extraction kit (Active Motif). Nuclear extracts were treated with an enzymatic shearing Cocktail for DNA digestion to release undissociated protein complexes ...
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bioRxiv - Genomics 2020Quote: ... the RevMAb Biosciences (clone RM362, at 1:300 dilution) and the mouse monoclonal recombinant AbFlex m6A antibody (rAb) from Active Motif (at 1:300 dilution). A mixture of reference DNA oligonucleotides that correspond to the position of the modified nucleotides was used to compare with antibody binding (the last base at the 5’ end of the oligonucleotide hybridizes with the modified base ...
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bioRxiv - Cell Biology 2024Quote: ... The H3K27Ac ChIP reactions were conducted with 10 μg of tissue chromatin and 4 μg of H3K27Ac antibody (Active Motif, cat # 39133). The H3K4me1 ChIP reactions were carried out by using 10 ug of tissue chromatin and 4 ul of H3K4me1 antibody (Active Motif ...
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bioRxiv - Genetics 2022Quote: ... Around 20 μg or 10 μg of chromatin were incubated overnight at 4°C with 10 μg H3.X/Y or 2.5 μg H3K9me3 antibody respectively (Active Motif-61161, Abcam-ab8898). Chromatinantibody extracted with incubated with protein G Dynal beads (Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2023Quote: ... and immunoprecipitation performed over night at 4 °C on a spinning wheel by adding 10 µl of the respective antibody (Anti H3K27Me3 #9733 Cell Signaling, Anti H3 #2650 Cell Signaling, Anti H3K56Ac #61061 Active Motif). Afterwards ...
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bioRxiv - Cell Biology 2024Quote: ... The chromatin was subjected to 3 x 10 minute cycles of ultrasonic shearing using a PIXUL multi-sample sonicator (Matchstick Technologies Inc., Kirkland, WA, and Active Motif, Carlsbad, CA).(Bomsztyk et al. ...
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bioRxiv - Cell Biology 2024Quote: ... The H3K4me1 ChIP reactions were carried out by using 10 ug of tissue chromatin and 4 ul of H3K4me1 antibody (Active Motif, cat # 39297). Libraries were prepared by a custom Illumina library with the standard Illumina PE adaptors (Bentley ...
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bioRxiv - Genomics 2021Quote: ... The DNA was denatured for 10 min at 95°C and immunoprecipitated overnight at 4°C with 4 μL of 5hmC monoclonal antibody (Active Motif Cat No 55010). To allow selective enrichment of immune-captured DNA fragments ...