Labshake search
Citations for Active Motif :
151 - 200 of 202 citations for SARS CoV 2 Spike Glycoprotein S2 aa 800 1000 His Tag E. coli since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Biophysics 2020Quote: ... and incubated with anti-Lamin A/C antibody 1:1000 (Active Motif, 39287) in block overnight ...
-
bioRxiv - Molecular Biology 2020Quote: ... Primary antibodies used are polyclonal Rabbit-anti-H3K23me1 (1:1000, Active Motif 30508001), polyclonal Rabbit-anti-H3K23me2 (1:1000 ...
-
bioRxiv - Molecular Biology 2020Quote: ... Cells were incubated with rabbit polyclonal antibodies against H3K9me3 (1:1000; Active Motif) in the 1XPBS for 1 hour at room temperature ...
-
bioRxiv - Microbiology 2022Quote: ... 1:1000 dilution rabbit anti-human acetyl histone H3 (Active Motif, Cat. 39040), and 1:500 dilution mouse anti-human β-actin (Cell Signaling ...
-
bioRxiv - Neuroscience 2021Quote: ... Primary and secondary antibodies used were: PARP1 (1:1000, 39559, RRID: AB_2793257, Active Motif), Anti-poly-ADP-ribose binding reagent (1:1000 ...
-
bioRxiv - Cell Biology 2021Quote: ... mouse or rabbit anti-H3K27ac (1:250; 39085, Active Motif; 1:1000; ab4729, Abcam), rabbit anti-Lamin B1 (1:100 ...
-
bioRxiv - Genetics 2021Quote: ... Primary antibodies used in this paper were rabbit anti-CID (1:1000, Active motif), rabbit anti-SPC105R (1:4000 ...
-
bioRxiv - Genomics 2023Quote: ... 2 μg of anti-His2Av (Active Motif 61686) were added to each immunoprecipitation for spike-in purposes ...
-
bioRxiv - Cancer Biology 2023Quote: ... Pol II ser 2 phos (Active Motif C1984), yH2AX (abcam ab11174) ...
-
bioRxiv - Molecular Biology 2023Quote: ... and 2 µg H3K9me3 antibody (39161, Active Motif). Samples were incubated for 3h at 4°C ...
-
bioRxiv - Genomics 2019Quote: ... transferred to PVDF membranes and probed with primary antibodies to BAF170 (Active Motif, 1:1000) and Histone H4 (1:6000 ...
-
bioRxiv - Cancer Biology 2023Quote: 2 × 107 cells for H3K27ac (Active Motif, Cat # 39133) and 3 × 107 cells for IRF4 (Santa Cruz Biotechnology ...
-
bioRxiv - Cancer Biology 2023Quote: ... and Negative Primer Set 1 and 2 (Active Motif) as positive and negative controls for p-STAT4 binding (see table S1 for sequences) ...
-
bioRxiv - Molecular Biology 2020Quote: ... Immunoblots were stained overnight with the following primary antibodies: Anti-PARP1 (1:1000 Active motif, 39559), Anti-DNMT1 (1:1000 ...
-
CENP-C regulates centromere assembly, asymmetry and epigenetic age in Drosophila germline stem cellsbioRxiv - Cell Biology 2020Quote: ... the following antibodies were used: rabbit anti-CENP-A (CID) antibody (Active Motif 39719; 1:1000), sheep anti-CENP-C (Dattoli et al ...
-
bioRxiv - Systems Biology 2023Quote: ... Antibodies were used at the following concentrations: Anti H3K27ac 1:1000 (Active Motif Cat No. 39685), and /or Anti H3K27me3 1:1000 (Active Motif Cat No ...
-
bioRxiv - Molecular Biology 2022Quote: ... along with 2 µg of anti-H2Av (Active Motif, #61686) [which recognizes Drosophila-specific histone variant H2Av in spike-in chromatin] ...
-
bioRxiv - Genomics 2021Quote: ... 2 μg for anti-CTCF (Active Motif (Carlsbad, CA, USA) 61312) ...
-
bioRxiv - Genomics 2021Quote: ... 0.5% Triton X-100) and 2 μL 5mC antibody (Active Motif 61479) were added ...
-
bioRxiv - Genomics 2023Quote: ... then incubated with 2 µg/mL dilution 5hmC antibody (Active Motif, 39092) overnight at 4°C in a humidified chamber ...
-
bioRxiv - Developmental Biology 2023Quote: ... mouse anti-MEIS1/2/3 (0.5 µg/ml, 39795, Active Motif, RRID:AB_2750570), rabbit anti-DHRS3 (1:500 ...
-
bioRxiv - Cancer Biology 2021Quote: ... and a p300 antibody (Clone NM11, 1:200 for ChIP, 1:1000 for IB, 1:100 for IP) were purchased from Active Motif. The antibody against cMyc-AcK148 was prepared in a previous study 28 ...
-
bioRxiv - Cell Biology 2023Quote: ... ‘H3K9Me3 antibody 2’ refers to 13969 (CST) and the H3K27Me3 to 39155 (Active Motif). This preceded binding of secondary antibody and CUT&Tag-IT™Assembled pA-Tn5 Transposomes ...
-
bioRxiv - Genetics 2019Quote: ... and mouse monoclonal anti-H3K27me3 antibody (Active Motif MABI0323, Lot 17019020; 2 µL per IP). Immunocomplex was captured by Protein A-conjugated sepharose beads (for rabbit antibodies ...
-
bioRxiv - Molecular Biology 2019Quote: ... and 2 µl of appropriate antibody (H3K27me2/3, Active Motif 39536, or GFP, MBL 598) was added and incubated with rotation at 4 °C overnight ...
-
bioRxiv - Cell Biology 2020Quote: The peptides were introduced into axons using 2 μL Chariot protein transfection system (Active Motif) only in the distal axon compartment of compartmented Campenot chambers immediately after R280H virus removal at DIV2 ...
-
bioRxiv - Cancer Biology 2021Quote: ... which had pre-incubated with 2 μg of H3K27me2/me3 antibody (39435, Active Motif, USA) or IgG (Abcam ...
-
bioRxiv - Cancer Biology 2022Quote: ... and rotated at 4°C overnight with 2-5 ug of antibody (H3K27ac, Active Motif Cat #39133 ...
-
bioRxiv - Developmental Biology 2022Quote: ... and H3K9ac antibody (Rep 1-1.4 ug or Rep 2-5 ug) (Active Motif, 39917). Enrichment was calculated relative to the geometric mean of two genes highly enriched for both H3K79me2 and H3K9ac in human (h ...
-
bioRxiv - Cancer Biology 2023Quote: ... and rotation at 4°C overnight with 2 ug of antibody (H3K27ac, #39133, Active Motif). Transcription factor ChIP-Seq was performed as previously described11 with 2-5 ug of antibody was used per immunoprecipitation (POU2F2 ...
-
bioRxiv - Biophysics 2023Quote: ... melanogaster Schneider cells in presence of 2 µg of anti-His2Av antibody (Active Motif 61686) for each immunoprecipitation ...
-
bioRxiv - Immunology 2023Quote: ... IgG (isotype control) or H4K20me1 samples: 2 µg of the anti-pol II (Active Motif) or 2 µg of isotype control (IgG ...
-
bioRxiv - Molecular Biology 2022Quote: ... The following antibodies and dilutions were used: anti-Esrrb (PP-H6705-00, Perseus Proteomics, 1:1000) and anti-Histone H3 (61476, Active Motif, 1:5000).
-
bioRxiv - Genomics 2021Quote: ... The resulting sheared chromatin fragments in a size range between 200 to 500 base pairs were incubated with H3K27me3 (Millipore #07-473,1:1000; Active Motif #39160, 1:500) or H4K20me3 (Abcam ...
-
bioRxiv - Molecular Biology 2023Quote: ... All incubations with primary antibodies were performed for either 1h at room temperature or overnight at 4°C with the following antibodies for H3K9me3 (Active Motif, 1:1000), DAXX (Santa Cruz ...
-
bioRxiv - Microbiology 2024Quote: ... The primary antibodies were used at the following dilutions: anti-Cas9 (1:1000 in 5% milk/PBS-T, Active Motif, clone 7A9-3A3); anti-EF1α (1:20,000 in 1% milk/PBS-T ...
-
bioRxiv - Bioengineering 2023Quote: ... and mouse anti-MEIS 1/2/3 antibody (1:200, clone 9.2.7, 39796, Active Motif, RRID:AB_2750570). Secondary antibodies (raised in donkey ...
-
bioRxiv - Genetics 2023Quote: ... and nuclei were resuspended in 50 μl transposition mix (25 μl 2× transposition buffer (Active Motif), 10 μl transposase (Active Motif) ...
-
bioRxiv - Immunology 2021Quote: ... for 30 minutes at 37°C followed by 2 μl proteinase K (Active Motif, 0.2 mg/ml) at 65°C for 2 hours ...
-
bioRxiv - Molecular Biology 2021Quote: ... Coupled antibody-Dynabeads were prepared by adding Dynabeads protein A/G and 2 μg antibody [H3K9ac (Active Motif) or H3K27ac (Abcam)] to 100 μl ice-cold dilution buffer and rotating the tubes end-over-end at 4°C for 1 hour ...
-
bioRxiv - Genomics 2022Quote: ... aliquots of ∼500,000 cells were added to 20 µl Dynabeads Protein A with 2 µg anti-H3K27ac (Active Motif) and incubated with slow rotation at 4°C overnight ...
-
bioRxiv - Genomics 2019Quote: ... and 1x protease inhibitor) before being resuspended in 50 μL of Buffer 2 containing 0.5 μL antibody (H3K27ac from Active Motif, 39122 ...
-
bioRxiv - Molecular Biology 2021Quote: ... Total RNA samples were subjected to immunoprecipitation with m6A specific antibody (m6A-1, Active Motif, #91261; m6A-2, Abcam, #ab151230) as previously described5 ...
-
bioRxiv - Immunology 2023Quote: Approximately 2 million BMDCs were processed according to the ChIP-IT Express Enzymatic Shearing and ChIP protocol (Active Motif, #53009) as previously described75 ...
-
bioRxiv - Developmental Biology 2021Quote: ... IP sample was incubated with 1 µg H3K4me3 antibody (in-house) (89) and 2 µg H3K27me3 antibody (Active Motif, Cat 61017) overnight with rotation at 4℃ ...
-
Transcription factor LSF facilitiates lysine methylation of α-tubulin by microtubule-associated SET8bioRxiv - Biochemistry 2019Quote: ... Two μg of each peptide and 2 μg of purified wild type or mutant SET8 or full-length SETD2 (Active Motif) were incubated with radioactively labeled [3H] AdoMet at room temperature overnight ...
-
bioRxiv - Biophysics 2021Quote: ... Supplementary Figure 12 and Supplementary Movie 8 show data of a 2% formaldehyde fixed C127 cell immune-labelled with rabbit anti-histone H3K4me3 primary antibodies (Active Motif) and goat-anti rabbit Alexa Fluor 488 secondary antibodies (ThermoFisher) ...
-
bioRxiv - Molecular Biology 2021Quote: ... Proteins produced in vitro were subjected to interaction assays for 2 hours at 4°C under mild rotation with Drosophila nuclear extracts or recombinant human CTD (Active Motif). Bound proteins were washed 4 times and resuspended in Laemmli buffer for western-blot analysis ...
-
bioRxiv - Immunology 2019Quote: ... Blots were incubated over night at 4°C with the following primary antibodies diluted in 2 % skim milk powder/PBS-T: H3K27ac (1:1,000, rabbit, Active Motif, 39133) and H3 (1:10,000 ...
-
bioRxiv - Immunology 2020Quote: ... and then incubated for 2 hours at room temperature with respective primary antibodies (at dilutions of 1:100): H3K27me3 (39155, Active Motif); Ubiquityl-histone H2A Lys119 (8240 ...