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Citations for Active Motif :
1 - 50 of 53 citations for Respiratory Syncytial Virus A Glycoprotein G Human Fc tag since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2020Quote: ... Recombinant Human DNMT1 with an N-terminal GST tag was purchased from Active Motif and Pierce Glutathione Magnetic Beads (Thermo Scientific ...
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bioRxiv - Cancer Biology 2024Quote: CUT&Tag assays were performed using CUT&Tag-IT assay kit (Active Motif) on 50,000 CD34+ cells according to manufacturer’s instructions ...
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bioRxiv - Cell Biology 2023Quote: Cut&Tag was performed using a Complete CUT&Tag-IT Assay Kit (Active Motif) according to manufacturer’s instructions ...
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bioRxiv - Cell Biology 2023Quote: CUT&Tag libraries were prepared using the CUT&Tag-IT Assay Kit (Active Motif, 53160) following to the manufacturer’s protocol ...
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bioRxiv - Genomics 2022Quote: ... The basal kit for CUT&Tag used was a CUT&Tag-IT Assay Kit (Active Motif). After washing the zona-free blastocysts with phosphate-buffered saline containing 0.01% (w/v ...
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bioRxiv - Genomics 2023Quote: ... The basal kit for CUT&Tag was a CUT&Tag-IT Assay Kit (Active Motif, Carlsbad, CA). Dig-Wash buffer and Dig-300 buffer in the kit were supplemented with 0.01% (w/v ...
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bioRxiv - Cell Biology 2022Quote: CUT&Tag-IT assay kit (Active Motif) was used on 3,000 HSC according to manufacturer’s instructions ...
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bioRxiv - Neuroscience 2023Quote: ... 100,000-125,000 nuclei were used for each CUT&Tag reaction using the CUT&Tag IT Assay Kit (Active Motif, #53610) per manufacturer’s instructions ...
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bioRxiv - Neuroscience 2022Quote: ... centrifuged 500 g for 5 min and washed a final time with 1 mL 1X CUT&Tag wash buffer (from CUT&Tag IT Assay Kit, Active Motif, #53610) or 1 mL CUT&RUN 1X wash buffer (1 mL HEPES pH 7.5 ...
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bioRxiv - Neuroscience 2022Quote: ... and the CUT&Tag-IT Assay Kit (Active Motif) following the manufacture’s manual ...
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bioRxiv - Genetics 2024Quote: CUT&Tag analysis of NaBu treated CCD841 cells and mouse intestinal samples were performed using CUT&Tag-IT™ assay kits (Active Motif # 53160, 53170). Approximately 30 mg of tissue was homogenized per reaction by first chopping frozen tissue into smaller pieces in a petri dish with a razor blade on dry ice ...
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bioRxiv - Neuroscience 2023Quote: ... nuclei pellet was washed once with 1 mL Nuclei Wash Buffer and then once with 1 mL of 1X CUT&Tag Wash Buffer (from Active Motif CUT&Tag IT Assay kit). Remove CUT&Tag Wash Buffer ...
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bioRxiv - Biophysics 2022Quote: ... and P1 virus was generated from cells transfected with FuGENE transfection reagent (Active Motif) according to the manufacturer’s instructions ...
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bioRxiv - Genomics 2023Quote: ... 0.5 million cells were processed using the CUT&Tag-IT kit (Active Motif) as per manufacturer’s instructions and the resulting libraries were paired-end sequenced on a NextSeq500 platform (Illumina ...
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bioRxiv - Genomics 2022Quote: ... we used CUT&Tag-IT™ assay kit (Cat. No. 53160) from Active Motif® ...
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bioRxiv - Biochemistry 2023Quote: ... and Protein G beads (Active motif, #53014) were added for an additional 2 hours ...
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bioRxiv - Cancer Biology 2020Quote: ... 30μl of Protein G Magnetic Beads (Active Motif) were washed twice with 500μl CoIP buffer and incubated with Antibody-containing lysat for 1 hour at 4°C with gentle rotation ...
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bioRxiv - Plant Biology 2019Quote: ... Added antibody-conjugated protein G beads (Active Motif 61085 for Pol II Ser5p ...
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bioRxiv - Neuroscience 2022Quote: CUT&Tag was performed from 450k nuclei using the Cut&TagIT Kit following manufacturer’s protocol (Active Motif).
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bioRxiv - Molecular Biology 2021Quote: Recombinant Flag-tagged human G9a (Active Motif, 31410), human calpain 1 (Novus ...
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bioRxiv - Cell Biology 2021Quote: ... At the same time protein G agarose beads (Active Motif) were washed and blocked with 5% BSA in ChIP buffer and left to rotate O/N in 4°C ...
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bioRxiv - Genomics 2023Quote: ... Human Negative Control Primer Set 1 (Active Motif, #71001), Human Negative Control Primer Set 3 (Active Motif ...
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bioRxiv - Genomics 2023Quote: ... Human Positive Control Primer Set MYT1 (Active Motif, #71007), Human Negative Control Primer Set 1 (Active Motif ...
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bioRxiv - Genomics 2023Quote: ... Human Negative Control Primer Set 3 (Active Motif, #71023). For analysis ...
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bioRxiv - Neuroscience 2024Quote: Cells from proliferating iMOPs and iMOP-derived neurons were harvested for and processed using the CUT&Tag-IT Assay Kit (Active Motif) described by the manufacturer ...
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bioRxiv - Molecular Biology 2022Quote: Cleavage under targets and tagmentation (CUT&Tag [35]) was performed for three biological replicates per experimental condition using the CUT&Tag-IT Assay Kit (Anti-Rabbit) (Active Motif, 53160) according to the manufacturer’s instructions with some modifications ...
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bioRxiv - Genetics 2022Quote: ... the nuclei suspension was diluted in a 1X volume of 1X Wash Buffer from a CUT&Tag-IT Assay Kit (Active Motif 53160). To estimate the yield of nuclei ...
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bioRxiv - Microbiology 2022Quote: ... parasites were centrifuged 2000xg for 10min and the parasite pellet used directly with the CUT&Tag-IT Assay Kit (Active Motif 53160). Our experimental sample used TgBDP13xHA parasites and 1ul of a 1:50 dilution of the rabbit anti-HA primary antibody (Cell Signaling 3724T) ...
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bioRxiv - Microbiology 2022Quote: ... Parasite pellets were then processed as per the manufacturer’s protocol (CUT&Tag-IT™ Assay Kit, Anti-Rabbit, Active motif, Cat no. 53160). Libraries were sequenced by Azenta at PE 2×150 bp.
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bioRxiv - Immunology 2021Quote: ... Chromatin was pre-cleared using 30 μl protein G agarose beads (Active Motif) pre-blocked with BSA per manufacturer’s instructions ...
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bioRxiv - Cell Biology 2024Quote: ... recombinant human PHD2(EGLN1) was purchased from Active Motif (USA, #81065) and HIF1α-ODD peptide (DLDLEALAPYIPADDDFQL ...
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bioRxiv - Molecular Biology 2021Quote: ... Immunoprecipitations for H2A.Z and HA-tag were performed similarly on benzonase-treated yeast cell extract using 36µl Protein-A sepharose beads coupled with 6µl of appropriate antibody (Anti-H2A.Z, Active Motif 39647; Anti-HA tag, Abcam ab9110). Control immunoprecipitations (mock ...
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bioRxiv - Molecular Biology 2023Quote: CUT&Tag was performed as described previously (Kaya-Okur et al., 2020; Kaya-Okur et al., 2019) using the CUT&Tag-IT Assay Kit from Active Motif (Catalog No. 53160) and following manufacturer’s instructions ...
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bioRxiv - Cell Biology 2023Quote: ... Immune complexes were collected using 20 μL of protein G magnetic beads (Active Motif). After elution from the beads ...
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bioRxiv - Microbiology 2022Quote: ... 1:1000 dilution rabbit anti-human histone H3 (Active Motif, Cat. 61799), 1:1000 dilution rabbit anti-human acetyl histone H3 (Active Motif ...
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bioRxiv - Molecular Biology 2024Quote: ... H3K27ac histone modification data was generated from liver sinusoidal endothelial (LSEC) and liver-resident immune cryopreserved cells using the Cut&Tag-IT Assay kit (Active Motif, H3K27ac antibody cat#39133). Library products were assessed by Agilent Bioanalyzer HS DNA assays (Agilent Technologies ...
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bioRxiv - Plant Biology 2019Quote: ... Added antibody-conjugated protein G beads (Active Motif 61085 for Pol II Ser5p, Active Motif for Pol II Ser2p and Ab817 for total Pol II ...
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bioRxiv - Immunology 2020Quote: ... Chromatin precipitation was performed using rabbit anti-human H3K4me3 IgG Ab (Active Motif) as described previously [48] ...
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bioRxiv - Microbiology 2022Quote: ... 1:1000 dilution rabbit anti-human acetyl histone H3 (Active Motif, Cat. 39040), and 1:500 dilution mouse anti-human β-actin (Cell Signaling ...
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bioRxiv - Molecular Biology 2020Quote: ... The lysates were transferred to a new tube with 50 μl Protein-G Dynabeads (Active Motif) and further incubated for 1 hr before following the manufacturer’s recommendations for washing of the beads and library preparation ...
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bioRxiv - Genetics 2019Quote: ... containing either 5 μg human liver or embryonic kidney nuclear extracts (Active Motif 36042; 36033), 2 μg of either anti c/EBPbeta (CEBPB ...
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bioRxiv - Molecular Biology 2021Quote: ... Coupled antibody-Dynabeads were prepared by adding Dynabeads protein A/G and 2 μg antibody [H3K9ac (Active Motif) or H3K27ac (Abcam)] to 100 μl ice-cold dilution buffer and rotating the tubes end-over-end at 4°C for 1 hour ...
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bioRxiv - Neuroscience 2019Quote: ... In vitro cleavage assays were performed by adding 50nM human recombinant Ago2 (expressed in baculovirus, Active Motif) in a reaction mixture containing 25mM Hepes-KOH pH 7.5 ...
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The G2-phase enriched lncRNA SNHG26 is necessary for proper cell cycle progression and proliferationbioRxiv - Molecular Biology 2021Quote: ... qPCR was performed using human positive and negative control qPCR primer sets from Active Motif (Supplementary Table S6). Furthermore ...
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bioRxiv - Genomics 2024Quote: Human islet ATAC-seq libraries were prepared following the Active motif ATAC prep kit (Active motif catalog# 53150). Briefly ...
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bioRxiv - Neuroscience 2020Quote: ... then placed on rotator and incubated overnight at 4°C with 25 uL of Protein G beads and 4 uL (1 mg/mL) anti-RNA polymerase II (102660; Active Motif). Immune complexes were sequentially washed according to kit instructions ...
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bioRxiv - Developmental Biology 2022Quote: ... One negative control primer pair was used (Unt12, Human negative control primer set 1, Active Motif, catalog number 71001) as well as one positive control (DPF1 for RUNX2 ...
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bioRxiv - Synthetic Biology 2021Quote: ... half of each original cell aliquot was incubated with Dynabeads protein-G conjugated with 5 µg of rabbit polyclonal to Histone H3K27me3 (Active motif 39155) or rabbit polyclonal to Histone H3 (acetyl K27 ...
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bioRxiv - Neuroscience 2021Quote: The rest of the ChIP experiment (beginning from “Protein G Agarose Bead Preparation”) was carried out using the reagents and protocols from the Low Cell ChIP-Seq Kit (Active Motif, 53084). In brief ...
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bioRxiv - Molecular Biology 2021Quote: ... qPCR was performed using human positive and negative control qPCR primer sets from Active Motif (Additional file 1: Table S5). Immunoprecipitated material and input chromatin were submitted to the Genomics Core Facility (GCF ...