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Citations for Active Motif :
151 - 199 of 199 citations for Recombinant Rabbit IL21 Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2023Quote: ... samples were incubated with 3 µg of rabbit anti-H3K9ac antibody (Active Motif; Carlsbad, CA) overnight at 4°C with rocking ...
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bioRxiv - Cell Biology 2024Quote: ... We used a rabbit anti-Cid/CENP-A antibody (Active Motif cat. 39713, 1:50) and rabbit anti-IgG as negative control (1:100) ...
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bioRxiv - Molecular Biology 2020Quote: ... The histone proteins and peptide used for antibody competition were histone H3K23me3 (Active motif, 31264), histone H3K9me3 (Active motif ...
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bioRxiv - Molecular Biology 2022Quote: ... Nuclear protein fractions were prepared by using Nuclear Complex co-IP kit (Active Motif #5400), according to the manufacturer ...
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bioRxiv - Plant Biology 2019Quote: ... Added antibody-conjugated protein G beads (Active Motif 61085 for Pol II Ser5p, Active Motif for Pol II Ser2p and Ab817 for total Pol II ...
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bioRxiv - Cell Biology 2020Quote: The peptides were introduced into axons using 2 μL Chariot protein transfection system (Active Motif) only in the distal axon compartment of compartmented Campenot chambers immediately after R280H virus removal at DIV2 ...
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bioRxiv - Cancer Biology 2024Quote: Nuclear proteins were extracted using the Nuclear Complex Co-IP Kit (Active Motif, Carlsbad, CA), following the manufacturer’s instructions ...
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bioRxiv - Evolutionary Biology 2020Quote: ... using 100,000-150,000 FANS-separated nuclei and anti-H3K27ac antibody (rabbit polyclonal, Active Motif cat# 39133). We employed the “native” ChIP protocol (N-ChIP ...
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bioRxiv - Cell Biology 2019Quote: ... rabbit anti-Histone H3ac(pan-acetyl) polyclonal antibody(Active Motif, WB 0.2μg/ml, Catalog No: 61637,61638), rabbit anti-Histone H3 polyclonal antibody(Proteintech ...
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CENP-C regulates centromere assembly, asymmetry and epigenetic age in Drosophila germline stem cellsbioRxiv - Cell Biology 2020Quote: ... the following antibodies were used: rabbit anti-CENP-A (CID) antibody (Active Motif 39719; 1:1000), sheep anti-CENP-C (Dattoli et al ...
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bioRxiv - Neuroscience 2023Quote: ... were coated with antibodies (Cell Signaling Technology Rabbit anti-H3K27me3 Cat#9733, RRID:AB_2616029 and Active Motif Rabbit anti-H3K4me3 Cat# 39159 ...
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bioRxiv - Molecular Biology 2020Quote: ... The lysates were transferred to a new tube with 50 μl Protein-G Dynabeads (Active Motif) and further incubated for 1 hr before following the manufacturer’s recommendations for washing of the beads and library preparation ...
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bioRxiv - Physiology 2019Quote: ... Ishikawa cell nuclear and cytoplasmic protein fractions were extracted using Nuclear Extraction Kit (Active Motif, Belgium) according to manufacturer’s instructions ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: Nuclear proteins were isolated from RAW264 cells using Nuclear Extract Kit (Active Motif, Carlsbad, CA, USA) according to the manufacturer’s protocols ...
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bioRxiv - Cell Biology 2021Quote: ... Histone modifications were detected with the following primary antibodies: rabbit polyclonal anti-H2AT120ph antibody (Active Motif, 39391) at a 1:10 dilution ...
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bioRxiv - Developmental Biology 2022Quote: ... the following primary antibodies were used: rabbit anti-CENP-A (CID) antibody (Active Motif 39719; 1:500), sheep anti-CENP-C (Dattoli et al. ...
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bioRxiv - Neuroscience 2023Quote: ... and secondary antibody was guinea pig anti-rabbit (Active Motif, 105465 from CUT&TagIT kit, 1:100).
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bioRxiv - Molecular Biology 2021Quote: Nuclear proteins were prepared using fibroblasts at 80-90% confluency and the Nuclear Extract Kit (Active Motif), according to the manufacturer’s protocol.
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bioRxiv - Genomics 2019Quote: Extraction of nuclear proteins and coimmunopreciptation were performed using the Universal Magnetic Co-IP Kit (Active Motif). Briefly ...
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bioRxiv - Neuroscience 2021Quote: ... and incubated overnight with primary FOXG1 antibody detecting both FOXG1 monomers and dimers (Rabbit polyclonal FOXG1, Active Motif) diluted in 5% BSA in TBST (Tris buffered saline with 0.1% Tween-20) ...
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bioRxiv - Molecular Biology 2021Quote: ... Coupled antibody-Dynabeads were prepared by adding Dynabeads protein A/G and 2 μg antibody [H3K9ac (Active Motif) or H3K27ac (Abcam)] to 100 μl ice-cold dilution buffer and rotating the tubes end-over-end at 4°C for 1 hour ...
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bioRxiv - Genomics 2021Quote: ... Rabbit anti-H3K4me3 (1:100 for bulk profiling or 1:10 for single-cell experiments, Active Motif Cat# 39159), Rabbit anti-H3K36me3 (1:100 for bulk profiling or 1:10 for single-cell experimemts ...
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bioRxiv - Neuroscience 2023Quote: ... or anti-Mouse AbberiorSTAR440 1:200 (Abberior, Göttingen, Germany) and anti-Rabbit Chromeo505 1:200 (Active Motif, Carlsbad, USA)) in 1X Roti®-ImmunoBlock in dark condition at room temperature for 1 hr ...
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bioRxiv - Cancer Biology 2021Quote: ... cells were harvested and nuclear protein complex lysates were prepared using the Nuclear Complex Co-IP Kit (Active Motif). Co-immunoprecipitation with two biological replicates was performed according to the manufacturer’s instructions ...
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bioRxiv - Genomics 2022Quote: ... aliquots of ∼500,000 cells were added to 20 µl Dynabeads Protein A with 2 µg anti-H3K27ac (Active Motif) and incubated with slow rotation at 4°C overnight ...
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bioRxiv - Genetics 2023Quote: ... The collected supernatants were incubated overnight with Protein A dynabeads (Life technology, 10001D) associated with H3K27Ac antibody (Active Motif). Next the input sample and chromatin-beads complexes sample were digested ...
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bioRxiv - Molecular Biology 2023Quote: ... The western blots of the chromatin-associated proteins were performed with anti-RNA pol II CTD phospho Tyr1 (Active Motif), anti-Rpb1 (manufactured in our laboratory ...
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bioRxiv - Genetics 2021Quote: ... Coverslips were removed and slides were washed in PBS with 0.1% Triton X-100 (PBST) (3X 10 minutes each) and subsequently incubated with primary rabbit anti-H3K9me3 (Active Motif #39765) antibodies (at 1:500 dilution ...
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bioRxiv - Biophysics 2021Quote: ... Supplementary Figure 12 and Supplementary Movie 8 show data of a 2% formaldehyde fixed C127 cell immune-labelled with rabbit anti-histone H3K4me3 primary antibodies (Active Motif) and goat-anti rabbit Alexa Fluor 488 secondary antibodies (ThermoFisher) ...
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bioRxiv - Genomics 2020Quote: ... The following primary antibodies were purchased and used for immunofluorescence and CUT&RUN27,28 followed by sequencing: Rabbit anti-H3K4me3 (Active motif, 39159), Rabbit anti-H3K27me3 (Active Motif ...
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bioRxiv - Cancer Biology 2020Quote: ... The membranes were blocked in 5 % milk/TBST for 1 h followed by primary antibody blotting at 4 °C overnight incubation – 1: 2000 rabbit anti-hmC (cat# 39069, Active Motif). The next day the blots were washed with TBST followed by incubation with secondary 1:2000 anti-rabbit-HRP for 1 h ...
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bioRxiv - Immunology 2019Quote: ... Blots were incubated over night at 4°C with the following primary antibodies diluted in 2 % skim milk powder/PBS-T: H3K27ac (1:1,000, rabbit, Active Motif, 39133) and H3 (1:10,000 ...
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bioRxiv - Neuroscience 2022Quote: ... ChIP reactions were carried out overnight at 4 °C with 3 µg of sheared chromatin and 10 μg of anti-H3K27me3 rabbit polyclonal antibody (39155, Active Motif) or anti-mouse IgG as negative control (115-001-003 ...
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bioRxiv - Cancer Biology 2021Quote: ... The level of protein binding to DNA consensus sequence was then assessed by ELISA using the TransAM NF-kB Family kit (Active Motif) according to manufacturer’s instructions.
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bioRxiv - Neuroscience 2021Quote: ... The sonicated lysate was added to 20 µl Dynabeads Protein A with 1.5 ug anti-H3K27ac (Active Motif, #39685 Mouse Monoclonal) and incubated with slow rotation at 4°C overnight ...
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bioRxiv - Neuroscience 2020Quote: ... then placed on rotator and incubated overnight at 4°C with 25 uL of Protein G beads and 4 uL (1 mg/mL) anti-RNA polymerase II (102660; Active Motif). Immune complexes were sequentially washed according to kit instructions ...
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bioRxiv - Molecular Biology 2020Quote: ... Beads were washed three times with PBS and bound proteins were recovered by adding Laemmli buffer and then analysed by immunoblotting with anti-DNMT1 antibody (Active Motif).
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bioRxiv - Physiology 2022Quote: ... protein from a subset of muscles was fractionated into nuclear and cytoplasmic components using a Nuclear Extract Kit (Active Motif; 40010) according to manufacturer’s instructions ...
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bioRxiv - Neuroscience 2019Quote: ... The membrane was incubated with the following primary antibodies diluted in 5% nonfat dry milk/TBS-T overnight at 4°C: rabbit polyclonal anti-5-hydroxymethylcytosine antibody (Active Motif, 39769) at 1:5000 and mouse monoclonal anti-5-methylcytosine antibody (Active Motif ...
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bioRxiv - Molecular Biology 2022Quote: Cleavage under targets and tagmentation (CUT&Tag [35]) was performed for three biological replicates per experimental condition using the CUT&Tag-IT Assay Kit (Anti-Rabbit) (Active Motif, 53160) according to the manufacturer’s instructions with some modifications ...
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bioRxiv - Microbiology 2023Quote: ... Beads were resuspended in 100 μl of IP/wash buffer and coated with 5 μg of rabbit anti-m6A antibody (Active motif, 61495) for 45 min at room temperature with rotation ...
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bioRxiv - Molecular Biology 2021Quote: ... and carried out from OSCs according to Fabry and colleagues (Fabry et al., 2019) using commercially available rabbit polyclonal anti-H3K9me3 (Active Motif, cat # 39161) and rabbit polyclonal anti-H3K4me2 (Merck Millipore ...
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bioRxiv - Microbiology 2022Quote: ... Parasite pellets were then processed as per the manufacturer’s protocol (CUT&Tag-IT™ Assay Kit, Anti-Rabbit, Active motif, Cat no. 53160). Libraries were sequenced by Azenta at PE 2×150 bp.
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bioRxiv - Biochemistry 2021Quote: ... rabbit polyclonal anti-Pre6 (gift from D. Wolf, 1:5000) and anti-Rpb1 (RNA Pol II) monoclonal mouse antibody (Active Motif, 4H8, 1:2000). For rabbit primary antibodies ...
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bioRxiv - Molecular Biology 2021Quote: ... Immunoprecipitations for H2A.Z and HA-tag were performed similarly on benzonase-treated yeast cell extract using 36µl Protein-A sepharose beads coupled with 6µl of appropriate antibody (Anti-H2A.Z, Active Motif 39647; Anti-HA tag, Abcam ab9110). Control immunoprecipitations (mock ...
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bioRxiv - Plant Biology 2020Quote: Histone proteins were extracted either from in liquid grown seedlings or from leaf tissue with a Histone Purification Kit (Active Motif, Cat No. 40025) using manufacturing instruction with some modifications ...
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bioRxiv - Cell Biology 2021Quote: ... Equal intensity of different RNA probes were mixed with equal molar of different C/EBPα proteins individually in EMSA buffer B1 (Active Motif, Cat. No. 37480), stabilizing solution D (Active Motif ...
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bioRxiv - Physiology 2022Quote: ... Chromatin preparations were subjected to ChIP using a ChIP-IT High Sensitivity Kit and Protein G Agarose Prepacked Columns (Active Motif, Carlsbad, CA, USA) using a PPARα (Abcam Ab24509 ...
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bioRxiv - Neuroscience 2021Quote: ... The following primary antibodies were diluted in blocking buffer and applied to cells overnight at 4°C to label key histone modifications associated with epigenetic regulation: rabbit H3K9me3 (1:500; Active Motif, 39765; Carlsbad, CA, USA); mouse H3K4me1 (1:100 ...