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Citations for Active Motif :
101 - 131 of 131 citations for Rat Cell Lysates since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2020Quote: Fibroblast cells were lysed in ice cold buffer (PBS, 0.05% Tween 20, pH 7.4) containing protease inhibitors (Active motif, 37491) and phosphatase inhibitor (Active motif ...
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bioRxiv - Genetics 2020Quote: ... containing primary antibody (rabbit anti-H3K9me3, Abcam ab8898; rabbit anti-H3K27me3, Cell Signaling Technology C36B11; rabbit anti-H3K4me3 Active Motif 39159 ...
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bioRxiv - Immunology 2020Quote: ... Nuclear extract from HuT78 cells (endogenous SP140) was prepared following manufacturer’s protocol (Active Motif nuclear extract and co-IP kit). Protein concentration was determined by Bradford Assay (Pierce) ...
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bioRxiv - Cell Biology 2020Quote: ... Sandwich ELISA to quantify phosphorylated IκBα in infected and non-infected cells at 0-120 minutes after infection (Function ELISA IκBα, S32/S36, Active Motif). iii ...
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bioRxiv - Neuroscience 2022Quote: ChIP-seq libraries were constructed from FACS-enriched RGCs (100,000 cells for each library) using an H3K27me3 antibody (1:100, Active Motif) or normal rabbit IgG (1:100 ...
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bioRxiv - Cancer Biology 2023Quote: ChIP assays at the HES1 promoter were performed in HT29 cells using the CHIP-IT kit (Active Motif, Carlsbad, USA). Sonicated chromatin was immunoprecipitated with antibodies against IgG (sc-3739 ...
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bioRxiv - Immunology 2024Quote: ... and total RNA fractions were prepared from A549 cells according to the RNA Subcellular Isolation Kit (Active Motif, Cat. #25501). cDNA was prepared as described above and qPCR was performed to analyze VILMIR expression in both cellular fractions ...
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bioRxiv - Immunology 2021Quote: ... Antibody of interest was added to sonicated chromatin aliquot and mixed (anti-H3K27ac Cell Signaling, Catalog # 8173, 5μl; anti-H3K27me3 Active Motif, Catalog # 39155 ...
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bioRxiv - Biophysics 2021Quote: ... Supplementary Figure 12 and Supplementary Movie 8 show data of a 2% formaldehyde fixed C127 cell immune-labelled with rabbit anti-histone H3K4me3 primary antibodies (Active Motif) and goat-anti rabbit Alexa Fluor 488 secondary antibodies (ThermoFisher) ...
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bioRxiv - Plant Biology 2021Quote: ... 30 ml cell suspension were sonicated for 5 min (5s on/10s off, 60% amplitude) with a sonicator (EpiShear, Active Motif) equipped with a ¼” microtip probe ...
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bioRxiv - Cancer Biology 2022Quote: ... Nuclear extraction of MDA-MB-231 cells was done according to manufactureŕs instructions (Universal Magnetic Co-IP Kit, Active Motif, 54002). Imaging flow cytometry analysis was conducted on an Amnis Imagestream Mk (>100,000 events ...
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bioRxiv - Genetics 2020Quote: ... Chr13.1-cl and Chr13.2-cl KO ES cells was determined by native ChIP-seq and anti-H3K9me3 serum (Active Motif, #39161) as described previously (45) ...
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bioRxiv - Biophysics 2019Quote: ... and the mix was incubated overnight at 4 ºC with antibodies against MeCP2 (D4F3, Cell Signalling) plus spike-in antibody (Active Motif). For ChIP-seq library preparation ...
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bioRxiv - Molecular Biology 2023Quote: ... the cells were incubated with anti-5mC antibody (1:200 diluted in 0.5% goat serum + PBST, 39649, Active Motif, CA, USA) at 4 °C overnight and then washed three times with PBST for 5 min each ...
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bioRxiv - Genetics 2023Quote: ... membranes were incubated overnight with the following antibodies diluted at 1:2,500: anti-H3 (clone 1B1B2) (Cell Signalling Technology, #14269]) anti-H3K9ac (Active Motif, #39137) anti-H3K27ac [Active Motif ...
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bioRxiv - Molecular Biology 2024Quote: ATAC-seq libraries were generated from liver sinusoidal endothelial (LSEC) and liver-resident immune cryopreserved cells using the ATAC-seq kit (Active Motif). H3K27ac histone modification data was generated from liver sinusoidal endothelial (LSEC ...
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bioRxiv - Cancer Biology 2024Quote: ... GSCs (10 million cells / Co-IP) were collected and prepared for nuclear extraction and purification using a nuclear extraction kit (Active Motif). Nuclear extracts were treated with an enzymatic shearing Cocktail for DNA digestion to release undissociated protein complexes ...
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bioRxiv - Synthetic Biology 2021Quote: ... half of each original cell aliquot was incubated with Dynabeads protein-G conjugated with 5 µg of rabbit polyclonal to Histone H3K27me3 (Active motif 39155) or rabbit polyclonal to Histone H3 (acetyl K27 ...
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bioRxiv - Neuroscience 2021Quote: The rest of the ChIP experiment (beginning from “Protein G Agarose Bead Preparation”) was carried out using the reagents and protocols from the Low Cell ChIP-Seq Kit (Active Motif, 53084). In brief ...
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bioRxiv - Molecular Biology 2023Quote: ... Antibodies against target histone modifications were used to detect respective bands (Anti H3K27Me3 #9733 Cell Signaling, Anti H3K9Me #ABE101 Merck Millipore, Anti H3K56Ac #61061 Active Motif, Anti H3K9Ac #39038 Active Motif ...
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bioRxiv - Immunology 2024Quote: Approximately >1 million astrocytes were used by cell preparation according to the ChIP-IT Express Enzymatic Shearing and ChIP protocol (Active Motif, #53009). Briefly ...
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bioRxiv - Molecular Biology 2024Quote: ... Primary antibodies (anti-BRD4, Cell Signaling Technologies Mouse mAb #63759 at 1:1000 dilution, anti-H3K27ac, Active Motif #39133, 1:1000 dilution) were prepared in blocking buffer and incubated on the sample overnight at 4 °C ...
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bioRxiv - Cancer Biology 2021Quote: The effect of CLEFMA on NF-κB p65-DNA interaction was assessed in the nuclear fraction of H441 and CCL151 cells by TransAM NF-κB p65 kit according to the manufacturer’s instructions (Active Motif, Carlsbad, CA, USA). A specific NF-κB inhibitor ...
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bioRxiv - Molecular Biology 2023Quote: Chromatin immunoprecipitation followed by high throughput sequencing (ChIP-seq)59 was performed from HeLa cells using the ChIP-IT High Sensitivity Kit from Active Motif (reference 53040) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2023Quote: ... cells were DNase treated and lysed in ice-cold ATAC seq lysis buffer from the ATAC-Seq Kit (Active Motif, Carlsbad, CA, USA) for 2 min ...
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bioRxiv - Molecular Biology 2021Quote: ... Immunoprecipitations for H2A.Z and HA-tag were performed similarly on benzonase-treated yeast cell extract using 36µl Protein-A sepharose beads coupled with 6µl of appropriate antibody (Anti-H2A.Z, Active Motif 39647; Anti-HA tag, Abcam ab9110). Control immunoprecipitations (mock ...
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bioRxiv - Molecular Biology 2020Quote: Chromatin immunoprecipitation followed by high throughput sequencing (ChIP-seq) (23) was performed from HeLa cells using the ChIP-IT High Sensitivity® kit from Active motif (ref #53040) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2019Quote: Isolation of nuclear protein extracts from ATI-like cells after 6 days of culture was performed with a Nuclear Extract Kit according to the manufacturer’s instructions (Active Motif, 40010, La Hulpe, Belgium) as described (Hofmann et al. ...
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bioRxiv - Genetics 2024Quote: CUT&Tag analysis of NaBu treated CCD841 cells and mouse intestinal samples were performed using CUT&Tag-IT™ assay kits (Active Motif # 53160, 53170). Approximately 30 mg of tissue was homogenized per reaction by first chopping frozen tissue into smaller pieces in a petri dish with a razor blade on dry ice ...
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bioRxiv - Neuroscience 2021Quote: ... The following primary antibodies were diluted in blocking buffer and applied to cells overnight at 4°C to label key histone modifications associated with epigenetic regulation: rabbit H3K9me3 (1:500; Active Motif, 39765; Carlsbad, CA, USA); mouse H3K4me1 (1:100 ...
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bioRxiv - Molecular Biology 2024Quote: ... H3K27ac histone modification data was generated from liver sinusoidal endothelial (LSEC) and liver-resident immune cryopreserved cells using the Cut&Tag-IT Assay kit (Active Motif, H3K27ac antibody cat#39133). Library products were assessed by Agilent Bioanalyzer HS DNA assays (Agilent Technologies ...