Labshake search
Citations for Active Motif :
151 - 200 of 392 citations for NGS ChIP Seq kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Developmental Biology 2020Quote: ... samples were Polytron minced and homogenized by douncing in Prep Buffer (ChIP-IT High Sensitivity Kit, Active Motif). They were then sonicated in 100 µl of sonication buffer (0.1%SDS ...
-
bioRxiv - Cancer Biology 2020Quote: ... The following day the beads were washed once with ChIP Buffer I and twice with ChIP Buffer II (Active Motif) before elution with 50mM Glycine (pH 2.8 ...
-
bioRxiv - Immunology 2023Quote: Approximately 2 million BMDCs were processed according to the ChIP-IT Express Enzymatic Shearing and ChIP protocol (Active Motif, #53009) as previously described75 ...
-
bioRxiv - Molecular Biology 2021Quote: Chromatin was prepared from flash-frozen liver tissue using the Active Motif ChIP-IT High Sensitivity kit (Active Motif), employing a modified protocol described in (47) ...
-
bioRxiv - Cancer Biology 2020Quote: ... ChIP Buffer I (Active Motif, La Hulpe, Belgium), 1X PIC and water ...
-
bioRxiv - Developmental Biology 2022Quote: ... For ChIP-M samples [CTCF (61311, Active Motif), PolII (ab817 ...
-
bioRxiv - Microbiology 2019Quote: ... Cell fixation was performed using the fixation reagents and steps from the Low Cell Chip Kit (Active Motif; Cat#53084) as described in the user manual ...
-
bioRxiv - Microbiology 2019Quote: Immunoprecipitation of both RNA-DNA hybrids (DRIP) and γH2A was performed using the ChIP-IT Enzymatic Express kit (Active Motif) with formaldehyde fixed chromatin samples ...
-
bioRxiv - Genomics 2020Quote: ... rabbit anti-CTCF (Active Motif, 61311, for microChIP-seq), rabbit anti-Rad21 (Santa Cruz ...
-
bioRxiv - Immunology 2024Quote: Approximately >1 million astrocytes were used by cell preparation according to the ChIP-IT Express Enzymatic Shearing and ChIP protocol (Active Motif, #53009). Briefly ...
-
bioRxiv - Genomics 2019Quote: ... ChIP-qPCR was performed by Active Motif (Carlsbad, CA) using an anti-PGR antibody (sc-7208 ...
-
bioRxiv - Molecular Biology 2022Quote: ... and PanH3Ac (5 μl/ChIP used, 39140, Active motif). Complexes were captured with a 1:1 mixture of magnetic Protein A and Protein G Dynabeads (Invitrogen ...
-
bioRxiv - Developmental Biology 2019Quote: ... Antibodies used for ChIP were: H3K27me3 (Active Motif, 39155) and H3K4me3 (Millipore 07-473).
-
bioRxiv - Microbiology 2020Quote: ... Pellets were resuspended in ChIP lysis buffer (Active Motif) supplemented with protease inhibitors and incubated on ice for 30 mins ...
-
bioRxiv - Cell Biology 2022Quote: ChIP was performed using anti-H3K27ac (Active Motif, 39133) and anti-histone H3 (Abcam ...
-
bioRxiv - Microbiology 2023Quote: ChIP-IT® Express Enzymatic (Active Motif, Cat#53035) kit was used for DRIP ...
-
bioRxiv - Developmental Biology 2022Quote: ... Tagmentation Master Mix was added to the pellet and reaction was incubated in a themomixer at 600-800 rpm set at 37C for 30 min as indicated by manufacturer (ATAC-Seq kit, 53150, Active Motif).
-
bioRxiv - Molecular Biology 2024Quote: ATAC-seq libraries were generated from liver sinusoidal endothelial (LSEC) and liver-resident immune cryopreserved cells using the ATAC-seq kit (Active Motif). H3K27ac histone modification data was generated from liver sinusoidal endothelial (LSEC ...
-
bioRxiv - Molecular Biology 2021Quote: ... After quenching with 0.5M glycine the HMGN-nucleosome complexes were immunoprecipitated using ChIP-IT Express kit (Active Motif, Cat. No. 53008) with either anti-HMGN1 ...
-
bioRxiv - Molecular Biology 2021Quote: ... The purified DNA was analyzed by ChIP-qPCR data relative to input using the mouse negative control and mouse positive control primer set Gapdh in the ChIP-IT qPCR analysis kit (Active Motif). Reaction conditions were programmed with initial denaturation at 50°C for 2 minutes and 95°C for 10 minutes followed by 40 cycles of 95°C for 15 seconds and 60°C for 1 minute ...
-
bioRxiv - Molecular Biology 2021Quote: DNA from whole brain and neuronal nuclei ChIP samples was purified with the Chromatin IP DNA purification kit (Active Motif, #58002) according to the manufacturer’s instructions ...
-
bioRxiv - Cancer Biology 2023Quote: PB NK cells were stimulated or not with IL-12 as previously described and Ch-IP was performed using ChIP-IT PBMCs Kit (Active Motif) according to the manufacturer’s instructions ...
-
bioRxiv - Microbiology 2022Quote: ... RNA-seq and data analysis was performed by Active Motif. In short ...
-
bioRxiv - Cancer Biology 2021Quote: ChIP experiments and library preparation was performed by Active Motif using the HistonePath workflow ...
-
bioRxiv - Molecular Biology 2021Quote: ... Primary antibodies used for ChIP included RNAPII (39497, Active Motif); hnRNPL (18354-1-AP ...
-
bioRxiv - Immunology 2019Quote: ... To each sonicated sample 300 µL ChIP Buffer (Active Motif) supplemented with PMSF (Carl Roth) ...
-
bioRxiv - Cancer Biology 2023Quote: ... and H3K27ac (Active Motif, 39133, rabbit polyclonal, 5 µg/ChIP). ChIP-seq libraries were prepped and sequenced as previously described (In vitro organoid ChIP-seq) ...
-
bioRxiv - Neuroscience 2019Quote: ... hiPSC-derived neurons were processed using the Active Motif ChIP-IT High Sensitivity kit according to the manufacturer’s recommendations (Active Motif, Cat. 53040). Briefly ...
-
bioRxiv - Cell Biology 2022Quote: ... Samples in the 300-500bp range were used for immunoprecipitation using the ChIP-IT High Sensitivity kit (Active Motif, cat. no. 53040). DNA was purified using a Zymo DNA Clean & Concentrator-5 kit (Zymo ...
-
bioRxiv - Developmental Biology 2023Quote: ... ChIP assay was done using SimpleChIP Plus Enzymatic Chromatin IP kit (magnetic beads) #9005 from CellSignalling and H3K27me3 antibody (Active Motif RRID:AB_2561020). ChIP samples were prepared with Kapa Hyper prep kit ...
-
bioRxiv - Immunology 2022Quote: ... RNA-Seq libraries and data analysis was performed by Active Motif. DESeq2 was used for differential analysis statistics ...
-
bioRxiv - Genomics 2019Quote: ... The ChIP-validated antibodies H3 pan-acetyl (Active Motif, cat # 39139) and Cas9 antibody (mAb ...
-
Identification of a PRC2 accessory subunit required for subtelomeric H3K27 methylation in NeurosporabioRxiv - Molecular Biology 2020Quote: H3K27me2/3 ChIP using anti-H3K27me2/3 antibody (Active Motif, 39536) was performed as previously described (12 ...
-
bioRxiv - Developmental Biology 2019Quote: ... lysed in RIPA buffer or Prep Buffer (ChIP-IT, Active Motif) and sonicated in Diagenode Bioruptor Pico ...
-
bioRxiv - Genetics 2021Quote: H3K27me2/3 ChIP using α-H3K27me2/3 antibody (Active Motif, 39536), which recognizes di- or trimethylated H3K27 ...
-
bioRxiv - Biochemistry 2021Quote: ... RNA ChIP-IT® was purchased from Active Motif (Tokyo, Japan).
-
bioRxiv - Molecular Biology 2022Quote: ... top up to 1ml for H3K27Ac ChIP (2.5ul, Active Motif, 39133); 3 ...
-
bioRxiv - Molecular Biology 2023Quote: ... Primary antibodies used for ChIP included RNAPII Ser5P (61,986, Active Motif); RNAPII Ser2P (61,984 ...
-
bioRxiv - Cancer Biology 2023Quote: ... we followed the ChIP Cell Fixation protocol provided by Active Motif. Cells were fixed by adding 1/10th volume of freshly prepared solution of 11% Formaldehyde (SIGMA #F-8775) ...
-
bioRxiv - Developmental Biology 2022Quote: ... ChIP was performed using the anti-H3K9me3 antibody (Active motif 39161) on sfGFP-GAF(N ...
-
bioRxiv - Cell Biology 2021Quote: ... while negative control was incubated with mouse IgG and input DNA without antibody using the CHIP—IT Express HT Kit from Active Motif (Cat no: 53018) according to the manufacturer’s instructions [Supplementary Table III] ...
-
bioRxiv - Cell Biology 2021Quote: ... while negative control was incubated with mouse IgG and input DNA without antibody using the CHIP—IT Express HT Kit from Active Motif (Cat no: 53018) according to the manufacturer’s instructions [Supplementary Table III] ...
-
bioRxiv - Molecular Biology 2021Quote: ATAC-seq service was performed on frozen liver tissue by Active Motif, on two biological replicates ...
-
bioRxiv - Cell Biology 2023Quote: Bulk RNA-seq service was provided by Active Motif (Carlsbad, CA, USA). As previously described 34 ...
-
bioRxiv - Neuroscience 2019Quote: ... ChIP assays were then conducted using antisera to CTCF (61311, Active Motif), histone H3K27ac (39133 ...
-
bioRxiv - Developmental Biology 2020Quote: ... All ChIPs were incubated overnight with the respective antibodies (CTCF Active Motif 61311 ...
-
bioRxiv - Molecular Biology 2023Quote: ... γH2AX (Active Motif # 39117; 1 mL per 1 mg DNA for CHIP); Senataxin (Novus biological # NB100-57542 ...
-
bioRxiv - Developmental Biology 2020Quote: H3K27me3 mintbody and H4K20me1 antibody used for nChIP-seq (Active Motif cat. 39727) were validated for sensitivity and specificity using the MODified Histone Peptide Array (ActiveMotif ...
-
bioRxiv - Genomics 2019Quote: ... H3K9Me3 ChIP was performed using an antibody from Active Motif (Cat No-39161), H3K27Me3 (Active Motif ...
-
bioRxiv - Molecular Biology 2019Quote: ... antibody was used for H3 ChIP assays and active motif 39140 (Active Motif) was used for pan-acetyl H3 ChIP assays.