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Citations for Active Motif :
301 - 350 of 408 citations for Mouse Stem Cell kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2021Quote: ... The supernatant was removed and the beads washed with 665µL of ChIP Buffer (from Active Motif kit) by inverting the tube several times ...
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bioRxiv - Molecular Biology 2021Quote: ATAC-seq was performed in biological duplicate using the ATAC-Seq Kit (Active Motif, catalog No.53150). Briefly ...
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bioRxiv - Neuroscience 2022Quote: For DNA Chromatin Immunoprecipitation (ChIP) we used the ChIP-IT High Sensitivity® kit from Active motif. Lysates were obtained as described in the RIP protocol ...
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bioRxiv - Neuroscience 2023Quote: ... and secondary antibody was guinea pig anti-rabbit (Active Motif, 105465 from CUT&TagIT kit, 1:100).
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bioRxiv - Cell Biology 2023Quote: ... and nuclei was extracted using ice-cold ATAC-lysis buffer (according to the kit protocol; Active Motif) and the pellet resuspended in the Tn5 tagmentation master mix ...
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bioRxiv - Molecular Biology 2023Quote: ... ChIP-seq libraries were constructed using the Next Gen DNA Library Kit (Active Motif, 53216 and 53264). Library quality was assessed using Agilent 2100 Bioanalyzer and Agilent High Sensitivity DNA assay ...
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bioRxiv - Cell Biology 2023Quote: ... ChIP was then performed using a ChIP-IT High Sensitivity kit (#53040, Active Motif, Carlsbad, CA, USA) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2020Quote: The following antibodies were used: EZH1 (Cell Signalling, 42088) EZH2 (Active Motif, 39933), H3K27m3 (Cell Signalling ...
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bioRxiv - Cancer Biology 2020Quote: ... cells were incubated with 1:200 dilution of PARP2 antibody (Active Motif, 39743) overnight at 4°C ...
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bioRxiv - Molecular Biology 2022Quote: ... 5 µl (50 ng) of Drosophila chromatin from S2 cells (Active Motif, #53083) was added as spike-in control ...
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bioRxiv - Molecular Biology 2020Quote: ... Cells were incubated with rabbit polyclonal antibodies against H3K9me3 (1:1000; Active Motif) in the 1XPBS for 1 hour at room temperature ...
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bioRxiv - Molecular Biology 2023Quote: ... 0.2 mM dithiothreitol (DTT)) and sonicated for cell lysis (Q120 Sonicator, Active Motif). After centrifugation for 1 h at 4 °C and 45,850 g ...
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bioRxiv - Microbiology 2020Quote: ... Chromatin immunoprecipitation was performed using the ChIP-IT Express Enzymatic kit according to the manufacturer’s directions (Active Motif). DNA was enzymatically sheared for 10 min ...
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bioRxiv - Cell Biology 2020Quote: Purified DNA fragments for ChIP-seq were prepared by using the ChIP-IT High Sensitivity Kit (Active Motif) and the related antibodies ...
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bioRxiv - Genomics 2019Quote: ... The libraries were generated with the Illumina Nextera DNA Library Prep kit and were sequenced by Active Motif and NIEHS Sequencing core by Illumina HiSeq 2000 and NextSeq 500 systems with 42bp paired-end reads on each sample ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... the nucleoproteins were extracted and detected using the Trans-AM NF-κB kits (Active Motif, Carlsbad, CA, USA).
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bioRxiv - Molecular Biology 2022Quote: ... ATAC-seq was performed as described previously (Buenrostro et al., 2015) using an ATAC-seq kit (Active Motif) as described by the manufacturer ...
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bioRxiv - Genomics 2022Quote: ... histones were extracted according to the recommended manufacturer’s one-step protocol using the Histone Extraction Kit (Active Motif). The protein concentration of the lysates was determined using the BCA Protein Assay Kit (Thermo Fisher Scientific) ...
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bioRxiv - Developmental Biology 2020Quote: ... samples were Polytron minced and homogenized by douncing in Prep Buffer (ChIP-IT High Sensitivity Kit, Active Motif). They were then sonicated in 100 µl of sonication buffer (0.1%SDS ...
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bioRxiv - Cell Biology 2021Quote: Cytoplasm and nucleus of keratinocytes were separated by using Nuclear Extract Kit (Active Motif, Inc., Carlsbad, CA, USA) following the manufacturer’s instructions ...
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Small Molecule Screen Identifies Pyrimethamine as an Inhibitor of NRF2-driven Esophageal HyperplasiabioRxiv - Cancer Biology 2022Quote: NRF2-ARE binding assay was performed with an ELISA-based TransAM NRF2 Assay Kit (Active Motif, Carlsbad, CA) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2023Quote: Chromatin immunoprecipitation (ChIP) assays were conducted using the ChIP-IT EXPRESS assay kit protocol (Active Motif, Carlsbad, CA). After exposure to Aβo ...
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bioRxiv - Neuroscience 2024Quote: Nrf2 activity was quantified using nuclear extracts and a TransAm NRF2 activity kit from Active Motif (cat# 50296) following the manufacturers included instructions.
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bioRxiv - Immunology 2023Quote: ... Chromatin immunoprecipitation (ChIP) was carried out using the ChIP-IT Express Enzymatic kit from Active Motif (Rixensart, Belgium). In brief ...
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bioRxiv - Molecular Biology 2023Quote: ... Chromatin for re-ChIP was prepared using the ChIP-IT High Sensitivity Kit (Active Motif, catalog no. 53040), as described above ...
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bioRxiv - Genomics 2024Quote: Human islet ATAC-seq libraries were prepared following the Active motif ATAC prep kit (Active motif catalog# 53150). Briefly ...
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bioRxiv - Immunology 2019Quote: Cytoplasmic extracts were generated by disrupting cells using hypotonic buffer (Active Motif, Carlsbad, CA) and pelleting the cell debris by centrifugation at 14000 × g for 10 min at 4 °C ...
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bioRxiv - Biophysics 2022Quote: ... and P1 virus was generated from cells transfected with FuGENE transfection reagent (Active Motif) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: ... Cells were lysed using 100 µl of ice-cold ATAC lysis buffer (Active Motif), followed immediately by addition of 1 ml pre-chilled wash buffer (10 mM Tris-HCl pH 7.4 ...
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bioRxiv - Molecular Biology 2020Quote: ... Global % 5mC levels were assayed using a Global DNA Methylation LINE-1 Kit (55017; Active Motif, Carlsbad, CA, USA) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2020Quote: ... IL-13 effects on NFκB p52 DNA binding was evaluated using the TransAM NFκB Transcription Factor Assay Kit (Active Motif). The samples were run in duplicates and absorbance was measured using a spectrophotometer at 450 nm.
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bioRxiv - Molecular Biology 2021Quote: Chromatin was prepared from flash-frozen liver tissue using the Active Motif ChIP-IT High Sensitivity kit (Active Motif), employing a modified protocol described in (47) ...
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bioRxiv - Cancer Biology 2023Quote: ... Nuclear fractions were extracted following the manufacturer’s instruction supplied with the Universal Magnetic co-IP kit (54002, Active Motif). 1000μg of proteins were immunoprecipitated using 5.0μg of antibody followed by protein G magnetic beads ...
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bioRxiv - Cell Biology 2020Quote: ... and nuclear extracts were isolated using the reagents and protocol provided in the Active Motif Nuclear Extract Kit (Active Motif). IL-13 effects on NFκB p52 DNA binding was evaluated using the TransAM NFκB Transcription Factor Assay Kit (Active Motif) ...
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bioRxiv - Developmental Biology 2019Quote: ... All H3K27ac ChIP experiments were processed as ChIP-seqs using the reagents from ChIP-IT High Sensitive kit (Active Motif). IPs were performed in parallel technical duplicates with 11 to 14µg of chromatin on each ...
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bioRxiv - Microbiology 2019Quote: Immunoprecipitation of both RNA-DNA hybrids (DRIP) and γH2A was performed using the ChIP-IT Enzymatic Express kit (Active Motif) with formaldehyde fixed chromatin samples ...
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bioRxiv - Neuroscience 2023Quote: ... 100,000-125,000 nuclei were used for each CUT&Tag reaction using the CUT&Tag IT Assay Kit (Active Motif, #53610) per manufacturer’s instructions ...
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bioRxiv - Genomics 2022Quote: ... at a concentration of 10 ng/million cells and spike-in antibody (Active Motif, 61686) at a concentration of 0.4 µg/million cells were added following lysis and sonication but prior to immunoprecipitation ...
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bioRxiv - Biochemistry 2021Quote: ... The cells were then washed in 1x PBS twice followed by histone extraction (Active Motif). The extracted histones were then western blotted for H2B-Ub levels (Cell Signaling ...
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bioRxiv - Genomics 2022Quote: ... 0.5 million G1-phase DLD-1 cells were processed according to manufacturer’s instructions (Active Motif). Samples were paired-end sequenced to obtain at least 107 reads ...
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bioRxiv - Cancer Biology 2023Quote: ... 150k cells were used per sample using the anti-H3K9me3 primary antibody (Active Motif, AB_2532132), the anti-γH2AX primary antibody (Abcam ...
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bioRxiv - Biophysics 2023Quote: ... melanogaster Schneider cells in presence of 2 µg of anti-His2Av antibody (Active Motif 61686) for each immunoprecipitation ...
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bioRxiv - Molecular Biology 2024Quote: ... 200’000 cells were used per sample using the anti-H3K9me3 primary antibody (Active Motif, AB_2532132). A homemade purified ProtA-Tn5 protein (3XFlag-pA-Tn5-Fl ...
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bioRxiv - Cancer Biology 2021Quote: ... The level of protein binding to DNA consensus sequence was then assessed by ELISA using the TransAM NF-kB Family kit (Active Motif) according to manufacturer’s instructions.
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bioRxiv - Molecular Biology 2021Quote: ... After quenching with 0.5M glycine the HMGN-nucleosome complexes were immunoprecipitated using ChIP-IT Express kit (Active Motif, Cat. No. 53008) with either anti-HMGN1 ...
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bioRxiv - Cancer Biology 2020Quote: ... Immunoprecipitated DNA and input DNA were then purified with phenol/chloroform extraction and amplified using GenoMatrix Whole Genome Amplification kit (Active Motif). Quantitative PCR was performed using PowerUP SYBR Green master mix on an ABI StepOnePlus real-time PCR instrument (Applied Biosystems) ...
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bioRxiv - Genomics 2020Quote: ... The DNA samples were processed for library preparation for next-generation sequencing by using a Next Gen DNA Library Kit (Active Motif) following the manufacturer’s manual ...
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bioRxiv - Molecular Biology 2021Quote: DNA from whole brain and neuronal nuclei ChIP samples was purified with the Chromatin IP DNA purification kit (Active Motif, #58002) according to the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2022Quote: ... Tagmentation Master Mix was added to the pellet and reaction was incubated in a themomixer at 600-800 rpm set at 37C for 30 min as indicated by manufacturer (ATAC-Seq kit, 53150, Active Motif).
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Alpha-1-antitrypsin binds to the glucocorticoid receptor with biological significance in macrophagesbioRxiv - Immunology 2022Quote: The binding of the p65 subunit of NFκB was quantified using the TransAM™ NFκB p65 kit (Active Motif, Carlsbad, CA), as we previously reported (Bai et al ...