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Citations for Active Motif :
101 - 150 of 435 citations for Human Nuclear Factor Of Activated T Cells 5 NFAT5 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2022Quote: ... The product was detected by using an anti-5-Hydroxymethylcytosine antibody (5 nM, Active Motif), Eu-Protein A (5 nM ...
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bioRxiv - Developmental Biology 2019Quote: ... Drosophila reads were aligned to dm6 Drosophila genome version and a normalization factor was then applied to zebrafish reads according to manufacturer’s protocol (Active Motif, 53093 and 61686). MACS2 (Zhang et al. ...
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bioRxiv - Plant Biology 2021Quote: ... 30 ml cell suspension were sonicated for 5 min (5s on/10s off, 60% amplitude) with a sonicator (EpiShear, Active Motif) equipped with a ¼” microtip probe ...
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bioRxiv - Neuroscience 2021Quote: Chromatin immunoprecipitation was performed using the Low Cell ChIP-Seq kit (#53048, Active Motif) following the manufacturer’s instructions ...
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bioRxiv - Pathology 2023Quote: ... Cells were lysed to ChIP-IT Express Magnetic Chromatin Immunoprecipitation kit (Active Motif 53008). Optimal sonication conditions were determined previously in order to obtain DNA fragments of about 500 bp ...
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bioRxiv - Cancer Biology 2024Quote: Purified core histone extracts prepared as described above were quantified for histone lysine trimethylation at lysine 9 and lysine 27 following ELISA protocols from Active motif (Catalogue # 53109 and 53106 ...
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bioRxiv - Cell Biology 2024Quote: ... recombinant human PHD2(EGLN1) was purchased from Active Motif (USA, #81065) and HIF1α-ODD peptide (DLDLEALAPYIPADDDFQL ...
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bioRxiv - Synthetic Biology 2021Quote: ... half of each original cell aliquot was incubated with Dynabeads protein-G conjugated with 5 µg of rabbit polyclonal to Histone H3K27me3 (Active motif 39155) or rabbit polyclonal to Histone H3 (acetyl K27 ...
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bioRxiv - Genomics 2023Quote: ... chromatin was extracted from CT26 cells using the Nucleosome Preparation Kit (cat: 53504, Active Motif). The enzymatic digestion time for this cell line was optimized to 30min and the mononucleosome preparation protocol was followed as per manufacturer’s recommendations ...
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bioRxiv - Microbiology 2022Quote: ... 1:1000 dilution rabbit anti-human histone H3 (Active Motif, Cat. 61799), 1:1000 dilution rabbit anti-human acetyl histone H3 (Active Motif ...
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bioRxiv - Genetics 2021Quote: ... 5 μg H3K27ac antibody (Active Motif, 39133), 4 μg H3K36me3 antibody (Abcam ...
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bioRxiv - Developmental Biology 2022Quote: ... or 5 µg EZH2 (#39901, Active Motif) antibody was used ...
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bioRxiv - Cell Biology 2021Quote: ... cells were re-suspended in Glycine Stop-Fix Solution (ChIP-IT Enzymatic Express Kit; Active Motif) and kept shaking for 5 mins at room temperature ...
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bioRxiv - Immunology 2020Quote: ... Chromatin precipitation was performed using rabbit anti-human H3K4me3 IgG Ab (Active Motif) as described previously [48] ...
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bioRxiv - Microbiology 2022Quote: ... 1:1000 dilution rabbit anti-human acetyl histone H3 (Active Motif, Cat. 39040), and 1:500 dilution mouse anti-human β-actin (Cell Signaling ...
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bioRxiv - Immunology 2019Quote: ... Blots were incubated over night at 4°C with the following primary antibodies diluted in 2 % skim milk powder/PBS-T: H3K27ac (1:1,000, rabbit, Active Motif, 39133) and H3 (1:10,000 ...
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bioRxiv - Developmental Biology 2022Quote: ... This DNA was then used in immunoprecipitation (IP) reactions using 5-Methylcytosine (5-mC) mouse monoclonal antibody (39649, Active Motif). Immunoprecipitated DNA and input control (pooled DNA that did not go through the IP step ...
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bioRxiv - Plant Biology 2020Quote: ... 5 μl of H3K36ac antibody (Active Motif: 39379) or 2.5 μl of H3K36me3 (Abcam ...
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bioRxiv - Cancer Biology 2022Quote: ... and 5-methylcytosine (5mC) (Active Motif, Cat. #39649). The secondary antibodies included ...
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bioRxiv - Molecular Biology 2022Quote: ... 5 mg anti-histone H3K27ac (Active Motif, 39133) antibody or 10 μL anti-CTCF (Cell Signaling ...
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bioRxiv - Genetics 2023Quote: ... 5 ug H3K79me2 (Active Motif, 39143, Lot 16918002), or 10 ul of RPB1 NTD D8L4Y (Cell Signaling Technology ...
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bioRxiv - Cancer Biology 2021Quote: Chromatin was isolated from 4T1 and 6DT1 cells using the ChIP-IT Express Enzymatic kit (Active Motif) according to manufacturers’s instructions as mentioned above ...
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bioRxiv - Microbiology 2023Quote: ... Histones were isolated from inhibitor treated or untreated cells using the histone extraction kit (Active Motif, 40028) and western blots performed ...
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bioRxiv - Genomics 2024Quote: ... and Illumina sequencing libraires were prepared using the Low Cell ChIP-Seq kit (Active Motif, Carlsbad, CA) according to the manufacturer instructions ...
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bioRxiv - Microbiology 2022Quote: ... Membranes were blocked in 5% milk in PBS-T for 1 h at room temperature and incubated overnight at 4°C with primary antibodies against Lamin AC (Active Motif 39287), Lamin B1 (Abcam ab16048) ...
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bioRxiv - Molecular Biology 2020Quote: ... Recombinant Human DNMT1 with an N-terminal GST tag was purchased from Active Motif and Pierce Glutathione Magnetic Beads (Thermo Scientific ...
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bioRxiv - Neuroscience 2019Quote: ... and rabbit polyclonal anti 5-hydroxymethylcytosine (Active Motif, 39769) at 1:20000 ...
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bioRxiv - Molecular Biology 2022Quote: ... and PanH3Ac (5 μl/ChIP used, 39140, Active motif). Complexes were captured with a 1:1 mixture of magnetic Protein A and Protein G Dynabeads (Invitrogen ...
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bioRxiv - Developmental Biology 2022Quote: ... 5 µg of either H3K27me3 (#39155, Active Motif, USA) or 5 µg EZH2 (#39901 ...
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bioRxiv - Genomics 2022Quote: ... and 5 µg of anti-H3K27Ac (Active Motif, 39133) were added overnight at 4°C with rotation ...
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bioRxiv - Genomics 2023Quote: ... or with 5 μg of H3K9me3 (Active Motif 39062) per 20 μl of protein A/G MagnaBeads (Merck Millipore) ...
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bioRxiv - Cancer Biology 2021Quote: Cells from two independent experiments were collected and open chromatin was assessed using an ATACseq kit (Active Motif, 53150). DNAseq library preparation was completed at the Oncogenomics Core Facility at the Sylvester Comprehensive Cancer Center ...
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bioRxiv - Neuroscience 2019Quote: ... and rabbit polyclonal anti 5-hydroxymethylcytosine (5hmC) (Active Motif, 39769) at 1:20000 ...
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bioRxiv - Neuroscience 2023Quote: ... and 2µg of 5-hmC (39791, Active Motif, Carlsbad, USA)) ...
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bioRxiv - Cancer Biology 2023Quote: ... and H3K27ac (Active Motif, 39133, rabbit polyclonal, 5 µg/ChIP). ChIP-seq libraries were prepped and sequenced as previously described (In vitro organoid ChIP-seq) ...
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bioRxiv - Developmental Biology 2024Quote: ... Mouse anti-5-methylcytosine monoclonal antibody (Active Motif, 1:500) was added for 2h at room temperature ...
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bioRxiv - Cancer Biology 2023Quote: ChIP assays at the HES1 promoter were performed in HT29 cells using the CHIP-IT kit (Active Motif, Carlsbad, USA). Sonicated chromatin was immunoprecipitated with antibodies against IgG (sc-3739 ...
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bioRxiv - Immunology 2024Quote: ... and total RNA fractions were prepared from A549 cells according to the RNA Subcellular Isolation Kit (Active Motif, Cat. #25501). cDNA was prepared as described above and qPCR was performed to analyze VILMIR expression in both cellular fractions ...
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bioRxiv - Genomics 2019Quote: ... or 0,5 µl of rabbit 5-Hydroxymethylcytosine antibody (Active motif 39769). To recover the antibody-bound DNA fragments ...
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bioRxiv - Molecular Biology 2023Quote: ... and incubated with 5 μg of anti-RELA (#36369, Active Motif), anti HA (H3663 ...
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bioRxiv - Neuroscience 2019Quote: ... In vitro cleavage assays were performed by adding 50nM human recombinant Ago2 (expressed in baculovirus, Active Motif) in a reaction mixture containing 25mM Hepes-KOH pH 7.5 ...
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bioRxiv - Molecular Biology 2024Quote: ATAC-seq libraries were generated from liver sinusoidal endothelial (LSEC) and liver-resident immune cryopreserved cells using the ATAC-seq kit (Active Motif). H3K27ac histone modification data was generated from liver sinusoidal endothelial (LSEC ...
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The G2-phase enriched lncRNA SNHG26 is necessary for proper cell cycle progression and proliferationbioRxiv - Molecular Biology 2021Quote: ... qPCR was performed using human positive and negative control qPCR primer sets from Active Motif (Supplementary Table S6). Furthermore ...
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bioRxiv - Neuroscience 2021Quote: The rest of the ChIP experiment (beginning from “Protein G Agarose Bead Preparation”) was carried out using the reagents and protocols from the Low Cell ChIP-Seq Kit (Active Motif, 53084). In brief ...
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bioRxiv - Developmental Biology 2020Quote: ... and then incubated with 5-methylcytosine antibody (1:200; Active Motif #39649) at 4 °C overnight ...
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bioRxiv - Genomics 2023Quote: ... We used 5 μg of polyclonal anti-H3K4me3 antibodies (Active motif, 39016) per MChIP-C experiment ...
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bioRxiv - Genomics 2023Quote: ... samples were incubated overnight with 5 μg of H3K27ac (Active Motif 39034) or with 5 μg of H3K9me3 (Active Motif 39062 ...
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bioRxiv - Molecular Biology 2023Quote: ... cells were DNase treated and lysed in ice-cold ATAC seq lysis buffer from the ATAC-Seq Kit (Active Motif, Carlsbad, CA, USA) for 2 min ...
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bioRxiv - Molecular Biology 2023Quote: Chromatin immunoprecipitation followed by high throughput sequencing (ChIP-seq)59 was performed from HeLa cells using the ChIP-IT High Sensitivity Kit from Active Motif (reference 53040) according to the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2022Quote: ... One negative control primer pair was used (Unt12, Human negative control primer set 1, Active Motif, catalog number 71001) as well as one positive control (DPF1 for RUNX2 ...