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Citations for Active Motif :
1 - 50 of 488 citations for GATOR2 complex protein WDR59 WDR59 Antibody since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2021Quote: ... cells were harvested and nuclear protein complex lysates were prepared using the Nuclear Complex Co-IP Kit (Active Motif). Co-immunoprecipitation with two biological replicates was performed according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2022Quote: ... Nuclear protein fractions were prepared by using Nuclear Complex co-IP kit (Active Motif #5400), according to the manufacturer ...
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bioRxiv - Cancer Biology 2024Quote: Nuclear proteins were extracted using the Nuclear Complex Co-IP Kit (Active Motif, Carlsbad, CA), following the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2023Quote: Nuclear complex Co-IP kit (Active Motif) was used for the Co-Immunoprecipitation according to manufacturer’s instructions for most protein interactions ...
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bioRxiv - Cancer Biology 2021Quote: ... or Nuclear Complex Co-IP Kit (Active Motif). Protein concentration was measured using the Pierce BCA Protein Assay Kit (Thermo Fisher Scientific) ...
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bioRxiv - Cancer Biology 2024Quote: ... the Nuclear Complex Co-IP Kit (Active Motif) was used according to manufacturer’s instruction ...
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bioRxiv - Cancer Biology 2024Quote: The Nuclear Complex Co-IP Kit (Active Motif) was utilized for co-immunoprecipitation on nuclear lysates ...
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bioRxiv - Pathology 2022Quote: ... 7.35 nM PRMT5/MEP50 complex (Active motif, Cat#31356), 0.4 mM N-terminal H4 peptides ...
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bioRxiv - Molecular Biology 2023Quote: ... MLL1 complex was purchased from Active Motif (catalog #31423).
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bioRxiv - Genomics 2020Quote: ... and assay was performed with Nuclear Complex Co-IP Kit (Active Motif) as instructed using antibodies against HA-tag (Thermo Fisher cat#26183) ...
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bioRxiv - Cancer Biology 2021Quote: Co-immunoprecipitiation was performed using the Nuclear Complex Co-IP Kit (Active Motif). 4T1 cells were seeded onto 15 cm tissue culture dishes at a seeding density of 5×106 cells/dish ...
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bioRxiv - Cancer Biology 2022Quote: ... nuclear extracts were prepared using the Nuclear Complex Co-IP kit (Active Motif); 2 μg of each extract was loaded for each experiment and histone H3 was examined as loading control ...
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bioRxiv - Cancer Biology 2022Quote: Nuclear extracts were prepared using the Nuclear Complex Co-IP kit (Active Motif) and 10 μg of each extract was loaded to examine protein levels of SMYD3 ...
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bioRxiv - Cancer Biology 2020Quote: ... the complex was biotin-labeled via incubation with recombinant sortase A5 enzyme (Active Motif) and a custom tri-glycine biotin peptide (Thermo Fisher) ...
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bioRxiv - Cancer Biology 2024Quote: 4T1 nuclear lysates were prepared using the Nuclear Complex Co-IP kit (Active Motif) according to the manufacturer’s instruction ...
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bioRxiv - Cancer Biology 2024Quote: ... and nuclear lysates were prepared using the Nuclear Complex Co-IP kit (Active Motif).
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bioRxiv - Immunology 2023Quote: ... and nuclear fraction was acquired using Nuclear Complex co-IP kit (Active Motif, 54001), which allows a gentle release of DNA-bound protein complexes in the nucleus ...
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bioRxiv - Molecular Biology 2023Quote: Subcellular fractionation was performed using the Nuclear Complex Co-IP kit (Active Motif, USA) according to manufacturer’s protocol until isolation of the nucleus ...
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bioRxiv - Molecular Biology 2021Quote: ... Coupled antibody-Dynabeads were prepared by adding Dynabeads protein A/G and 2 μg antibody [H3K9ac (Active Motif) or H3K27ac (Abcam)] to 100 μl ice-cold dilution buffer and rotating the tubes end-over-end at 4°C for 1 hour ...
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bioRxiv - Cancer Biology 2024Quote: Co-immunoprecipitation (co-IP) was performed using the Nuclear Complex Co-IP Kit (Active Motif). 6T1 Smarcd1 OE cells were seeded onto 15 cm tissue culture dishes at a seeding density of 5×106 cells per dish ...
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bioRxiv - Cancer Biology 2022Quote: ... nuclear fraction digestion and collection was performed using Nuclear Complex Co-IP Kit (Active Motif, 54001). 25µl per IP of GFP-Trap Magnetic Agarose beads (Chromotek ...
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bioRxiv - Pathology 2022Quote: Co-immunoprecipitation (Co-IP) was conducted using the Nuclear Complex Co-IP Kit (Active Motif, 54001) according to the manufacturer’s manual ...
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bioRxiv - Molecular Biology 2023Quote: Co-immunoprecipitation (Co-IP) was performed using the Nuclear Complex Co-IP kit (Active Motif, USA) and Dynabeads™ Protein G for Immunoprecipitation according to manufacturer’s protocol (Invitrogen ...
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bioRxiv - Developmental Biology 2024Quote: ... Co-immunoprecipitation was performed 48 h later using Nuclear Complex Co-IP kit (Active Motif #54001) according to kit instruction ...
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bioRxiv - Molecular Biology 2020Quote: ... The histone proteins and peptide used for antibody competition were histone H3K23me3 (Active motif, 31264), histone H3K9me3 (Active motif ...
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bioRxiv - Cancer Biology 2024Quote: ... Cell pellets used for fractionation were fractionated using the Nuclear Complex Co-IP kit from Active motif (#54001) according to their protocol.
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bioRxiv - Cell Biology 2024Quote: ... after which CTCF protein-RNA complexes were immunoprecipitated using the relevant antibody (anti-CTCF, Active Motif 61331). In parallel to the IP ...
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bioRxiv - Microbiology 2024Quote: ... Protein was harvested and separated via SDS-PAGE to western blot against LexA (antibody purchased from Active Motif). Intensities of intact and cleaved LexA bands were normalized to a background band that was unaltered by treatment using FIJI.
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bioRxiv - Genetics 2023Quote: ... The collected supernatants were incubated overnight with Protein A dynabeads (Life technology, 10001D) associated with H3K27Ac antibody (Active Motif). Next the input sample and chromatin-beads complexes sample were digested ...
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bioRxiv - Molecular Biology 2020Quote: ... Beads were washed three times with PBS and bound proteins were recovered by adding Laemmli buffer and then analysed by immunoblotting with anti-DNMT1 antibody (Active Motif).
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bioRxiv - Cancer Biology 2024Quote: ... Chromatin immunoprecipitation was performed using 15 ug of purified rabbit polyclonal YAP-specific antibody (US Biologicals, Y1200-01D) or rabbit IgG (control) and protein G magnetic beads (Active Motif). Immunoprecipitated chromatin-protein complexes were washed extensively with RIPA buffer (1x ...
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bioRxiv - Molecular Biology 2021Quote: ... Immunoprecipitations for H2A.Z and HA-tag were performed similarly on benzonase-treated yeast cell extract using 36µl Protein-A sepharose beads coupled with 6µl of appropriate antibody (Anti-H2A.Z, Active Motif 39647; Anti-HA tag, Abcam ab9110). Control immunoprecipitations (mock ...
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bioRxiv - Biochemistry 2023Quote: ... recombinant AGO2 protein (Active Motif) was purchased and serially diluted in Protein LoBind tubes (Eppendorf ...
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bioRxiv - Microbiology 2021Quote: Nuclear proteins were isolated from challenged A549 cells using a nuclear protein extraction kit (Active Motif) according to manufacturer’s specifications and protein concentration was determined using a bicinchoninic acid (BCA ...
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bioRxiv - Neuroscience 2023Quote: ... myelin basic protein (Active Motif, 31314) was employed as a substrate for recombinant CDKL5 ...
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bioRxiv - Molecular Biology 2020Quote: ... 0.8μM of purified DNMT1 protein (Active Motif) was incubated with 30000 cpm of 32P-labelled aptamers and single or double-stranded ...
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bioRxiv - Biochemistry 2023Quote: ... and Protein G beads (Active motif, #53014) were added for an additional 2 hours ...
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bioRxiv - Immunology 2024Quote: Recombinant Tn5 transposase protein (Active Motif #81284) lots were assembled with IDT custom oligos mosaic end (ME ...
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bioRxiv - Genomics 2022Quote: ... 1 μg anti-H2Av antibody (spike-in antibody, Active Motif), 10 ng spike-in chromatin (D.melanogaster ...
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bioRxiv - Cell Biology 2020Quote: The following commercially available proteins were used in this study: recombinant SETD2 (a.a. 1418-1714) protein (Active Motif, 31358), rabbit skeletal muscle actin (Cytoskeleton ...
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bioRxiv - Developmental Biology 2023Quote: ... the rest was incubated with primary antibody (H3K4me3 antibody: Cell signaling Technology, 9727; H3K9me3 antibody: Active Motif, 39161) -coated Dynabeads Protein A/G for overnight at 4°C ...
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bioRxiv - Cancer Biology 2020Quote: ... 30μl of Protein G Magnetic Beads (Active Motif) were washed twice with 500μl CoIP buffer and incubated with Antibody-containing lysat for 1 hour at 4°C with gentle rotation ...
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bioRxiv - Cell Biology 2024Quote: ... 30 μl protein-G magnetic beads (Active Motif) and 10 μg CTCF antibody (Active motif ...
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bioRxiv - Cancer Biology 2024Quote: ... primary antibody (Active Motif), secondary antibody ...
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bioRxiv - Cell Biology 2023Quote: ... recombinant CDKL5 and myelin basic protein (Active Motif, 31314) as a substrate were incubated in a kinase buffer (Cell Signaling ...
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bioRxiv - Genomics 2022Quote: ... 5 μg of antibody and 2 µg of spike-in antibody (Active Motif 61686) were bound to 50 μl protein A or protein G Dynabeads (Invitrogen ...
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bioRxiv - Cancer Biology 2020Quote: ... H3K27Ac antibody (39133, Active Motif), or NEUROD1 antibody (sc-1084 ...
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bioRxiv - Neuroscience 2024Quote: ... Antibodies for H3K4me3 (Active Motif), p300 (Developmental Studies Hybridoma Bank) ...
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bioRxiv - Cell Biology 2023Quote: ... H3K27ac antibody (Active Motif #39134), BRD4 antibody (Epicypher #13-2003 ...
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bioRxiv - Cancer Biology 2024Quote: ... and antibody (Active motif, 61686) were included to normalize SMC1A ChIP-seq ...