Labshake search
Citations for Active Motif :
351 - 400 of 505 citations for B Cell Activating Factor BAFF TNFSF13B Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Cell Biology 2024Quote: Primary antibodies used were: mAb to RNA Pol II CTD S5ph (1:200, rat, Active Motif, 61701); mAb to SUN2 (1:400 ...
-
bioRxiv - Neuroscience 2021Quote: ... and incubated overnight with primary FOXG1 antibody detecting both FOXG1 monomers and dimers (Rabbit polyclonal FOXG1, Active Motif) diluted in 5% BSA in TBST (Tris buffered saline with 0.1% Tween-20) ...
-
bioRxiv - Genetics 2020Quote: ... were pre-blocked in fresh blocking buffer (0.5% BSA in PBS) and incubated with 2.5 µL of polyclonal H3K9me3 antibody (AB_2532132, Active Motif) or Rabbit IgG (negative control ...
-
bioRxiv - Molecular Biology 2019Quote: ... using 10mg of antibody abcam ab992 together with 40ng of Drosophila melanogaster spike-in chromatin (Active motif 53083) and spike-in antibody (Active motif 61686) ...
-
bioRxiv - Molecular Biology 2021Quote: ... All antibodies used in this study have been previously reported as suitable for ChIP: H3K4me3 (39159, Active Motif), H3K27ac (39133 ...
-
bioRxiv - Genetics 2020Quote: ... or 15 µg of chromatin and 2.5 µg of H3K27ac and H3K9me3 antibody (Active Motif #3913, Diagenode #C15410193). Final DNA purification was performed using the GeneJET PCR Purification Kit (Thermo Scientific ...
-
bioRxiv - Cancer Biology 2021Quote: ... The following primary antibodies were used: anti-FOXA1 (GeneTex, catalog no. GTX100308 and Active motif, catalog no. 39837) and GATA3 (Assay Biotech ...
-
bioRxiv - Biochemistry 2020Quote: ... the spike-in chromatin and antibody were added in the reaction as per the manufacturer’s recommendation (Active Motif). The chromatin was incubated with antibodies at 4 °C overnight and then added to 30 μl of protein G-Dyna beads (Thermo Fisher Scientific ...
-
bioRxiv - Immunology 2020Quote: ... Lysates were incubated overnight at 4°C with 5 μg antibody against RNA Pol II (39097, Active Motif), RelA (A301-824A ...
-
bioRxiv - Biochemistry 2020Quote: ... the spike-in chromatin and antibody were added in the reaction as per the manufacturer’s recommendation (Active Motif). The chromatin was incubated with antibodies at 4 °C overnight and then added to 30 μl of protein G-Dyna beads (Thermo Fisher Scientific ...
-
bioRxiv - Systems Biology 2023Quote: ... 2 μg of spikein antibody and 20 ng of spike-in chromatin (Active Motif 61686 and 53083, respectively) were added to chromatin prepared from 2.5×106 MV411 cells 72 hours after RNP-mediated TF knockout ...
-
bioRxiv - Developmental Biology 2022Quote: ... They were then incubated overnight at 4C with the primary antibodies in PBST (Active motif (2AG-6F12-H4) anti-H3K9me3 (1:100) ...
-
bioRxiv - Genomics 2023Quote: ... incubated for 45 min at RT with primary antibody diluted in 1X PBS/1% BSA (H3K27me3, Active motif-61017 ...
-
bioRxiv - Genomics 2024Quote: ... Soluble chromatin fractions were incubated with antibodies (anti-H3K9me2, Abcam ab1220; anti-H3K9me3, Active Motif, catalog no 39161) overnight at 4°C ...
-
bioRxiv - Cancer Biology 2023Quote: 2 × 107 cells for H3K27ac (Active Motif, Cat # 39133) and 3 × 107 cells for IRF4 (Santa Cruz Biotechnology ...
-
bioRxiv - Genomics 2023Quote: ... the Low Cell ChIP-Seq Kit from Active Motif was used (53084) ...
-
bioRxiv - Genetics 2023Quote: ... and ∼1 × 107 cells for H3K27ac (Active Motif, 39133). Cells were crosslinked with 1% formaldehyde for 10 min at room temperature (RT ...
-
Transcription factor LSF facilitiates lysine methylation of α-tubulin by microtubule-associated SET8bioRxiv - Biochemistry 2019Quote: ... 1 mg of total HEK293T cellular extract was incubated with 5 μg of anti-SET8 antibody (Active Motif, 61009), anti-LSF antibody (Millipore,17-10252) ...
-
bioRxiv - Cancer Biology 2019Quote: ... Genomic DNA regions of interest were isolated using 4 ug of antibody against Histone H3K27me3 (clone: 39155, Active Motif) Complexes were washed ...
-
bioRxiv - Genomics 2021Quote: ... 2 μg of spike-in antibody and 20 ng of spike-in chromatin (Active Motif 61686 and 53083, respectively) were added to chromatin prepared from 2.5×106 MV411 cells 72 hours after RNP-mediated TF knockout ...
-
bioRxiv - Genomics 2021Quote: ... 2 μg of spike-in antibody and 20 ng of spike-in chromatin (Active Motif 61686 and 53083, respectively) were added to chromatin prepared from 2.5×106 MV411 cells 72 hours after RNP-mediated TF knockout ...
-
bioRxiv - Cancer Biology 2023Quote: ... After 1 hour blocking (3% donkey serum, 1% BSA, 0.3% Triton X-100, PBS) the H3.3K27M antibody (1:500, 61803, Active Motif) diluted in a blocking solution was added and incubated for 1 hour ...
-
bioRxiv - Cancer Biology 2023Quote: ... the bead/nuclei mixture was incubated overnight with a 1:100 dilution of primary antibody (H3K27ac (39133, Active Motif)) which was followed by secondary antibody (13-1047 ...
-
bioRxiv - Genetics 2023Quote: ... The collected supernatants were incubated overnight with Protein A dynabeads (Life technology, 10001D) associated with H3K27Ac antibody (Active Motif). Next the input sample and chromatin-beads complexes sample were digested ...
-
bioRxiv - Microbiology 2023Quote: ... The membranes were blocked in 5% milk and incubated with anti-CTCF antibody (Active Motif 61311; 1:1,000 dilution), anti-β-actin antibody (Sigma-Aldrich ...
-
bioRxiv - Cancer Biology 2024Quote: RAS GTPase activity in cell lysate of the WT CaSki or ΔPr-A9 cells was determined by RAS GTPase Chemi ELISA Kit (Active Motif, Carlsbad, CA, # 52097) according to the manufacturer protocol ...
-
bioRxiv - Synthetic Biology 2021Quote: ... Cells were then incubated in MAXBlock Blocking medium (Active Motif) supplemented with final concentrations of 0.1% Triton X-100 and 100 mM glycine for 20 minutes at RT ...
-
bioRxiv - Molecular Biology 2020Quote: ... cells were resuspended in 100ul chromatin sheering buffer (Active Motif) and placed on 96 well plate sealed with PCR film (MiniAMP Optical Adhesive Film ...
-
bioRxiv - Genomics 2020Quote: ... and incubated with the appropriate primary antibody for 1 h at room temperature (anti-RNAPII: 1:500, 61086, Active Motif; anti-H3K27ac ...
-
bioRxiv - Genomics 2019Quote: ... and 1x protease inhibitor) before being resuspended in 50 μL of Buffer 2 containing 0.5 μL antibody (H3K27ac from Active Motif, 39122 ...
-
bioRxiv - Cancer Biology 2020Quote: ... Genomic DNA regions of interest were isolated using 4 ug of antibody against H3K27Ac (Active Motif, cat# 39133, Lot# 28518012). Complexes were washed ...
-
bioRxiv - Neuroscience 2022Quote: ... 1:50 Dilutions of primary antibodies against the following active and repressive histone modifications were used: H3K4me3 (Active Motif, 39159), H3K27ac (Abcam ...
-
bioRxiv - Developmental Biology 2022Quote: ... This DNA was then used in immunoprecipitation (IP) reactions using 5-Methylcytosine (5-mC) mouse monoclonal antibody (39649, Active Motif). Immunoprecipitated DNA and input control (pooled DNA that did not go through the IP step ...
-
bioRxiv - Immunology 2019Quote: ... Protein-DNA complexes were immuno-precipitated using 3μg of H3K27ac antibody from the same lot for all ChIP experiments (Catalogue #39133; Active Motif) coupled to 50μl Protein G Dynabeads (Thermo Fisher ...
-
bioRxiv - Molecular Biology 2021Quote: ... Total RNA samples were subjected to immunoprecipitation with m6A specific antibody (m6A-1, Active Motif, #91261; m6A-2, Abcam, #ab151230) as previously described5 ...
-
bioRxiv - Cancer Biology 2019Quote: ... Genomic DNA regions of interest were isolated using 4 µg of antibody against H3K27Ac (Active Motif, cat# 39133, Lot# 01518010). Complexes were washed ...
-
bioRxiv - Genomics 2021Quote: ... 1:50 Dilutions of primary antibodies against the following active and repressive histone modifications were used: H3K4me3 (Active Motif, 39159), H3K27ac (Abcam ...
-
bioRxiv - Cancer Biology 2020Quote: ... Supershift analysis was carried out by antibodies to RUNX 1 or RUNX 3 (Active Motif cat# 39300 and 39301, respectively). The gel was exposed to X ray film and/or phosphorimaging screens ...
-
bioRxiv - Cancer Biology 2021Quote: ... Genomic DNA from in vitro methylated Jurkat cells were (Active Motif) served as positive control and genomic DNA from H1993 cells served as negative control ...
-
bioRxiv - Cancer Biology 2023Quote: ... we followed the ChIP Cell Fixation protocol provided by Active Motif. Cells were fixed by adding 1/10th volume of freshly prepared solution of 11% Formaldehyde (SIGMA #F-8775) ...
-
bioRxiv - Molecular Biology 2021Quote: ... precleared chromatin was incubated overnight at 4 °C with 5 μg of the following antibodies: H3K4me2 (39141; Active Motif, Carlsbad, USA), H3K9me3 (39161 ...
-
bioRxiv - Immunology 2022Quote: ... 1 × 105 BM pDCs sorted from Hdac3fl/fl control or Hdac3fl/fl CreERT2 littermates were bound to beads and were subjected for immunoprecipitation with primary anti-H3K27ac antibody (Active Motif) at 4°C overnight ...
-
bioRxiv - Genomics 2020Quote: ... The following primary antibodies were purchased and used for immunofluorescence and CUT&RUN27,28 followed by sequencing: Rabbit anti-H3K4me3 (Active motif, 39159), Rabbit anti-H3K27me3 (Active Motif ...
-
bioRxiv - Developmental Biology 2020Quote: ... ChIP-Seq of T7 and MED12 were done in the presence of Drosophila spike-in chromatin and antibody following the manufacturers’ protocol (Active Motif). ChIP-Seq library construction was done using NEBNext Ultra™ II DNA Library Prep Kit for Illumina (NEB ...
-
bioRxiv - Molecular Biology 2020Quote: ... Beads were washed three times with PBS and bound proteins were recovered by adding Laemmli buffer and then analysed by immunoblotting with anti-DNMT1 antibody (Active Motif).
-
bioRxiv - Cancer Biology 2021Quote: ... and a p300 antibody (Clone NM11, 1:200 for ChIP, 1:1000 for IB, 1:100 for IP) were purchased from Active Motif. The antibody against cMyc-AcK148 was prepared in a previous study 28 ...
-
bioRxiv - Cancer Biology 2020Quote: ... The membranes were blocked in 5 % milk/TBST for 1 h followed by primary antibody blotting at 4 °C overnight incubation – 1: 2000 rabbit anti-hmC (cat# 39069, Active Motif). The next day the blots were washed with TBST followed by incubation with secondary 1:2000 anti-rabbit-HRP for 1 h ...
-
bioRxiv - Immunology 2019Quote: ... Blots were incubated over night at 4°C with the following primary antibodies diluted in 2 % skim milk powder/PBS-T: H3K27ac (1:1,000, rabbit, Active Motif, 39133) and H3 (1:10,000 ...
-
bioRxiv - Immunology 2020Quote: ... and then incubated for 2 hours at room temperature with respective primary antibodies (at dilutions of 1:100): H3K27me3 (39155, Active Motif); Ubiquityl-histone H2A Lys119 (8240 ...
-
bioRxiv - Genomics 2022Quote: ... 0.25% NP-40) and incubated for 1 h with 10 μg/IP of mouse bridging antibody (Active Motif; 1 μg/μl) at 4°C with end-to-end rotation ...