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Citations for Active Motif :
1 - 50 of 107 citations for 7 4 Aminobenzamido 4 hydroxynaphthalene 2 sulfonic acid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2022Quote: ... and rotated at 4°C overnight with 2-5 ug of antibody (H3K27ac, Active Motif Cat #39133 ...
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bioRxiv - Cancer Biology 2023Quote: ... and rotation at 4°C overnight with 2 ug of antibody (H3K27ac, #39133, Active Motif). Transcription factor ChIP-Seq was performed as previously described11 with 2-5 ug of antibody was used per immunoprecipitation (POU2F2 ...
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bioRxiv - Molecular Biology 2022Quote: ... and incubated with 4-8 μg primary target antibody and 2 μg spike-in antibody (Active Motif, #61686) overnight at 4°C ...
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bioRxiv - Immunology 2019Quote: ... trimethylated H3 lysine 4 (Active Motif 39159), and anti-glutathione-S-transferase (GST ...
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bioRxiv - Cancer Biology 2022Quote: ... 4 µg H3K27ac antibody (Active Motif, 39133) was coupled to 40 µl Dynabeads M280 Anti Rabbit Magnetic beads (Thermo Fisher ...
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bioRxiv - Genomics 2024Quote: ... trimethyl histone 3 lysine 4 (Active Motif 39915), CTCF (Creative Diagnostics DMABT-H19813 ...
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bioRxiv - Genomics 2023Quote: ... were pre-coated with antibodies for 4 hours at 4°C and the following antibodies were used: H3K27ac (39685 – Active Motif); H3K9ac (C5B11-9549 – Cell Signalling Technology) ...
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bioRxiv - Molecular Biology 2021Quote: ... Proteins produced in vitro were subjected to interaction assays for 2 hours at 4°C under mild rotation with Drosophila nuclear extracts or recombinant human CTD (Active Motif). Bound proteins were washed 4 times and resuspended in Laemmli buffer for western-blot analysis ...
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bioRxiv - Developmental Biology 2020Quote: ... Beads were then resuspended in Blocking buffer and coated with antibodies for 4 h at 4°C (H4K20me1 [0.5 μg/IP], Active Motif cat. 39727). Once coated beads were magnet-separated and resuspended in 1 mL of lysate ...
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bioRxiv - Cancer Biology 2019Quote: ... or 4 μg of H3K27Ac antibody (Active Motif #39133). Libraries were prepared by Active Motif and sequenced on a NextSeq 500 to a depth of ∼50 M reads (Jefferson University Genomics Laboratory ...
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bioRxiv - Genomics 2021Quote: ... ChIP enrichment was performed by incubation for 16 h at 4°C with the following antibodies: 2 µg H3K27ac antibody (Active Motif #39133), 4 µg H3K4me1 antibody (Millipore 07-436) ...
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bioRxiv - Genomics 2021Quote: ... The DNA was denatured for 10 min at 95°C and immunoprecipitated overnight at 4°C with 4 μL of 5hmC monoclonal antibody (Active Motif Cat No 55010). To allow selective enrichment of immune-captured DNA fragments ...
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bioRxiv - Cancer Biology 2019Quote: ... overnight at 4 °C or H3K27ac (Active motif; Catalog no: 39133). The purified ChiP DNA was used to generate sequencing libraries using Hapa Hyper prep kit from Kapa Biosystems ...
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bioRxiv - Molecular Biology 2024Quote: ... Antibody (4 μl per sample) against H3K27ac (Active Motif, cat# 39133) was conjugated to magnetic beads (Sera-Mag ...
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bioRxiv - Molecular Biology 2023Quote: ... Histone deacetylases were inhibited with Romidepsin (4 nM; Active Motif, # 14083) or Panobinostat (20 nM ...
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bioRxiv - Developmental Biology 2020Quote: ... pre-cleared for 2 h followed by immunoprecipitation with primary antibodies for 12-16 hours at 4°C (H3K27ac, Active motif 39133; H3K27me3, CST 9733s). 5% of samples were used as input DNA ...
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bioRxiv - Molecular Biology 2021Quote: ... samples were incubated with 4 µg of Pol II antibody (Active Motif, 4H8) at 4°C overnight ...
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bioRxiv - Neuroscience 2023Quote: ... they were incubated overnight at 4°C with the primary antibody (5mC, Active Motif, cat.no ...
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bioRxiv - Systems Biology 2020Quote: ... Extracts were incubated overnight at 4°C with 5 μg of anti-H3K27ac (Active Motif) prebound to protein G Dynabeads (Thermo Scientific ...
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bioRxiv - Cell Biology 2020Quote: ... Primary antibodies (Histone H3 – Abcam ab1791, H3k27me3 – Active Motif – 39155 and TLR-4 -Abcam 76B357.1) were then diluted 1:1000 in blocking buffer and were incubated on a rocker overnight at 4C ...
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bioRxiv - Genomics 2020Quote: ... and immunoprecipitation was performed using 4 μg of the appropriate antibody (anti-CTCF: 61311, Active Motif; anti-RAD21 ...
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bioRxiv - Systems Biology 2019Quote: ... Permeabilized cells were incubated overnight at 4°C with 5ug of anti-H3K27me3 (Active Motif 39156) and then washed before incubating with protein A-MNase fusion protein (a gift from S ...
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bioRxiv - Cancer Biology 2021Quote: ... and genomic DNA regions of interest isolated using 4 μg of H3K27Ac antibody (Active Motif, cat# 39133). Complexes were washed ...
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bioRxiv - Molecular Biology 2020Quote: ... 4 - 10 µg of target antibodies and 2μg of spike-in antibody (anti-H2Av, Active Motif, #61686) were added and incubated on a rotator at 4°C overnight ...
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bioRxiv - Genomics 2020Quote: ... 5 - 33 μg of sonicated chromatin was incubated with 4 μg ChIP-validated CTCF antibody (Active Motif) overnight on an end-to-end rotator at 4°C ...
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bioRxiv - Bioengineering 2019Quote: ... for 4-6 hours at room temperature and incubated in MAXbind Staining Medium (Active Motif, catalog no. 15251) containing primary antibodies at a concentration of 10 μg/mL overnight at 4□C ...
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bioRxiv - Immunology 2020Quote: ... Lysates were incubated overnight at 4°C with 5 μg antibody against RNA Pol II (39097, Active Motif), RelA (A301-824A ...
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bioRxiv - Cancer Biology 2019Quote: ... Genomic DNA regions of interest were isolated using 4 ug of antibody against Histone H3K27me3 (clone: 39155, Active Motif) Complexes were washed ...
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bioRxiv - Biochemistry 2019Quote: ... at 4°C with Active Motif enzymatic shearing cocktail in 100 µl Complete Digestion Buffer (both from Active Motif), and centrifuged for 10 min ...
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bioRxiv - Biochemistry 2024Quote: ... ChIP was carried out overnight at 4°C using IgG control or H4ac Pan-acetylation antibody (Active Motif # 39243) with anti-rabbit Dynabeads blocked with BSA ...
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bioRxiv - Genomics 2019Quote: ... 5 million cells per replicate were incubated with 5 ug (Histone) or 7.5 ug (Oct4) antibody overnight at 4’C (antibodies: H3K9me3: abcam AB8898; H3K27Ac: Active Motif 39133 ...
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bioRxiv - Cancer Biology 2020Quote: ... Genomic DNA regions of interest were isolated using 4 ug of antibody against H3K27Ac (Active Motif, cat# 39133, Lot# 28518012). Complexes were washed ...
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bioRxiv - Cancer Biology 2019Quote: ... Genomic DNA regions of interest were isolated using 4 µg of antibody against H3K27Ac (Active Motif, cat# 39133, Lot# 01518010). Complexes were washed ...
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bioRxiv - Molecular Biology 2021Quote: ... precleared chromatin was incubated overnight at 4 °C with 5 μg of the following antibodies: H3K4me2 (39141; Active Motif, Carlsbad, USA), H3K9me3 (39161 ...
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bioRxiv - Neuroscience 2020Quote: ... then placed on rotator and incubated overnight at 4°C with 25 uL of Protein G beads and 4 uL (1 mg/mL) anti-RNA polymerase II (102660; Active Motif). Immune complexes were sequentially washed according to kit instructions ...
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bioRxiv - Molecular Biology 2019Quote: ... Isolated cells were picked in 1 μl of 1× PBS and transferred into 4.4 μl of lysing buffer containing 4 μl mTRAP™ Lysis Buffer (Active Motif) and 0.4 μl (10 ng ...
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bioRxiv - Biophysics 2019Quote: ... and the mix was incubated overnight at 4 ºC with antibodies against MeCP2 (D4F3, Cell Signalling) plus spike-in antibody (Active Motif). For ChIP-seq library preparation ...
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bioRxiv - Cancer Biology 2020Quote: ... The membranes were blocked in 5 % milk/TBST for 1 h followed by primary antibody blotting at 4 °C overnight incubation – 1: 2000 rabbit anti-hmC (cat# 39069, Active Motif). The next day the blots were washed with TBST followed by incubation with secondary 1:2000 anti-rabbit-HRP for 1 h ...
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bioRxiv - Neuroscience 2022Quote: ... ChIP reactions were carried out overnight at 4 °C with 3 µg of sheared chromatin and 10 μg of anti-H3K27me3 rabbit polyclonal antibody (39155, Active Motif) or anti-mouse IgG as negative control (115-001-003 ...
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bioRxiv - Microbiology 2022Quote: ... Membranes were blocked in 5% milk in PBS-T for 1 h at room temperature and incubated overnight at 4°C with primary antibodies against Lamin AC (Active Motif 39287), Lamin B1 (Abcam ab16048) ...
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bioRxiv - Neuroscience 2019Quote: ... The membrane was incubated with the following primary antibodies diluted in 5% nonfat dry milk/TBS-T overnight at 4°C: rabbit polyclonal anti-5-hydroxymethylcytosine antibody (Active Motif, 39769) at 1:5000 and mouse monoclonal anti-5-methylcytosine antibody (Active Motif ...
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bioRxiv - Molecular Biology 2020Quote: ... 5ug antibodies were added to the supernatant and incubated overnight at 4°C with gentle rotation (CTCF, ab70303; RAD21, ab992; H3K4me3, Millipore, 04-745; H3K27ac, Active Motif,39685.). Chromatin was pulled down by protein G Sepharose beads (#17061801 ...
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bioRxiv - Genetics 2020Quote: ... Each ChIP used 2μg sheared chromatin and was incubated overnight (16 hours) at 4°C with either 5μg of anti-H3K9ac (39137, Active Motif, Carlsbad, CA), or 5μg of anti-H3K27ac (39133 ...
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bioRxiv - Genetics 2019Quote: ... Binding reactions for super shift assays were performed for 30 min at 4-8°C using the Binding Buffer B-1 (Active Motif 37480) together with Stabilizing Solution D (Active Motif 37488 ...
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bioRxiv - Genetics 2019Quote: ... Binding reactions with 3 × 105 cpm of labeled probe was performed for 20 min at 4°-8°C using Binding Buffer C-1 (Active Motif 37484) together with Stabilizing Solution D (Active Motif 37488) ...
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bioRxiv - Cell Biology 2024Quote: ... The H3K27Ac ChIP reactions were conducted with 10 μg of tissue chromatin and 4 μg of H3K27Ac antibody (Active Motif, cat # 39133). The H3K4me1 ChIP reactions were carried out by using 10 ug of tissue chromatin and 4 ul of H3K4me1 antibody (Active Motif ...
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bioRxiv - Developmental Biology 2023Quote: ... and chromatin was incubated for 2h at 4°C with 5 μg of anti-H3K27ac antibody (Active Motif, Cat# 39133, Lot 01613007). Immuno-complexes were sequentially washed ...
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bioRxiv - Molecular Biology 2024Quote: ... 1 mL of diluted nucleosomes were incubated with 4 μL of anti-H3K27ac (Cell Signalling Technology D5E4) or 10 μL of anti-H2BK5ac (Active Motif 39123). Libraries were prepared for ChIP and matched input samples using the NEBNext Ultra II DNA library kit and indexed using NEBNext Multiplex Oligos ...
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bioRxiv - Genetics 2022Quote: ... Around 20 μg or 10 μg of chromatin were incubated overnight at 4°C with 10 μg H3.X/Y or 2.5 μg H3K9me3 antibody respectively (Active Motif-61161, Abcam-ab8898). Chromatinantibody extracted with incubated with protein G Dynal beads (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2024Quote: ... The H3K4me1 ChIP reactions were carried out by using 10 ug of tissue chromatin and 4 ul of H3K4me1 antibody (Active Motif, cat # 39297). Libraries were prepared by a custom Illumina library with the standard Illumina PE adaptors (Bentley ...