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Citations for Active Motif :
51 - 100 of 108 citations for 6 PHENYL 4 TRIFLUOROMETHYL 2 PYRIDONE since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2020Quote: ... 4 - 10 µg of target antibodies and 2μg of spike-in antibody (anti-H2Av, Active Motif, #61686) were added and incubated on a rotator at 4°C overnight ...
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bioRxiv - Genomics 2020Quote: ... 5 - 33 μg of sonicated chromatin was incubated with 4 μg ChIP-validated CTCF antibody (Active Motif) overnight on an end-to-end rotator at 4°C ...
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bioRxiv - Immunology 2020Quote: ... Lysates were incubated overnight at 4°C with 5 μg antibody against RNA Pol II (39097, Active Motif), RelA (A301-824A ...
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bioRxiv - Genomics 2022Quote: ... 5 μg of antibody and 2 µg of spike-in antibody (Active Motif 61686) were bound to 50 μl protein A or protein G Dynabeads (Invitrogen ...
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bioRxiv - Cell Biology 2023Quote: ... ‘H3K9Me3 antibody 2’ refers to 13969 (CST) and the H3K27Me3 to 39155 (Active Motif). This preceded binding of secondary antibody and CUT&Tag-IT™Assembled pA-Tn5 Transposomes ...
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bioRxiv - Cancer Biology 2019Quote: ... Genomic DNA regions of interest were isolated using 4 ug of antibody against Histone H3K27me3 (clone: 39155, Active Motif) Complexes were washed ...
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bioRxiv - Biochemistry 2019Quote: ... at 4°C with Active Motif enzymatic shearing cocktail in 100 µl Complete Digestion Buffer (both from Active Motif), and centrifuged for 10 min ...
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bioRxiv - Genetics 2019Quote: ... and mouse monoclonal anti-H3K27me3 antibody (Active Motif MABI0323, Lot 17019020; 2 µL per IP). Immunocomplex was captured by Protein A-conjugated sepharose beads (for rabbit antibodies ...
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bioRxiv - Molecular Biology 2019Quote: ... and 2 µl of appropriate antibody (H3K27me2/3, Active Motif 39536, or GFP, MBL 598) was added and incubated with rotation at 4 °C overnight ...
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bioRxiv - Cell Biology 2020Quote: The peptides were introduced into axons using 2 μL Chariot protein transfection system (Active Motif) only in the distal axon compartment of compartmented Campenot chambers immediately after R280H virus removal at DIV2 ...
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bioRxiv - Cancer Biology 2021Quote: ... which had pre-incubated with 2 μg of H3K27me2/me3 antibody (39435, Active Motif, USA) or IgG (Abcam ...
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bioRxiv - Developmental Biology 2022Quote: ... and H3K9ac antibody (Rep 1-1.4 ug or Rep 2-5 ug) (Active Motif, 39917). Enrichment was calculated relative to the geometric mean of two genes highly enriched for both H3K79me2 and H3K9ac in human (h ...
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bioRxiv - Immunology 2023Quote: ... IgG (isotype control) or H4K20me1 samples: 2 µg of the anti-pol II (Active Motif) or 2 µg of isotype control (IgG ...
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bioRxiv - Biophysics 2023Quote: ... melanogaster Schneider cells in presence of 2 µg of anti-His2Av antibody (Active Motif 61686) for each immunoprecipitation ...
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bioRxiv - Genomics 2019Quote: ... 5 million cells per replicate were incubated with 5 ug (Histone) or 7.5 ug (Oct4) antibody overnight at 4’C (antibodies: H3K9me3: abcam AB8898; H3K27Ac: Active Motif 39133 ...
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bioRxiv - Cancer Biology 2020Quote: ... Genomic DNA regions of interest were isolated using 4 ug of antibody against H3K27Ac (Active Motif, cat# 39133, Lot# 28518012). Complexes were washed ...
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bioRxiv - Cancer Biology 2019Quote: ... Genomic DNA regions of interest were isolated using 4 µg of antibody against H3K27Ac (Active Motif, cat# 39133, Lot# 01518010). Complexes were washed ...
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bioRxiv - Immunology 2020Quote: ... GenScript #A02038) and two SARS-CoV-2 spike Antibodies from Active Motif (AM002414, #91349; AM001414, #91361).
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bioRxiv - Bioengineering 2023Quote: ... and mouse anti-MEIS 1/2/3 antibody (1:200, clone 9.2.7, 39796, Active Motif, RRID:AB_2750570). Secondary antibodies (raised in donkey ...
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bioRxiv - Genetics 2023Quote: ... and nuclei were resuspended in 50 μl transposition mix (25 μl 2× transposition buffer (Active Motif), 10 μl transposase (Active Motif) ...
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bioRxiv - Molecular Biology 2021Quote: ... precleared chromatin was incubated overnight at 4 °C with 5 μg of the following antibodies: H3K4me2 (39141; Active Motif, Carlsbad, USA), H3K9me3 (39161 ...
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bioRxiv - Neuroscience 2020Quote: ... then placed on rotator and incubated overnight at 4°C with 25 uL of Protein G beads and 4 uL (1 mg/mL) anti-RNA polymerase II (102660; Active Motif). Immune complexes were sequentially washed according to kit instructions ...
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bioRxiv - Molecular Biology 2019Quote: ... Isolated cells were picked in 1 μl of 1× PBS and transferred into 4.4 μl of lysing buffer containing 4 μl mTRAP™ Lysis Buffer (Active Motif) and 0.4 μl (10 ng ...
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bioRxiv - Biophysics 2019Quote: ... and the mix was incubated overnight at 4 ºC with antibodies against MeCP2 (D4F3, Cell Signalling) plus spike-in antibody (Active Motif). For ChIP-seq library preparation ...
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bioRxiv - Cancer Biology 2020Quote: ... The membranes were blocked in 5 % milk/TBST for 1 h followed by primary antibody blotting at 4 °C overnight incubation – 1: 2000 rabbit anti-hmC (cat# 39069, Active Motif). The next day the blots were washed with TBST followed by incubation with secondary 1:2000 anti-rabbit-HRP for 1 h ...
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bioRxiv - Neuroscience 2022Quote: ... ChIP reactions were carried out overnight at 4 °C with 3 µg of sheared chromatin and 10 μg of anti-H3K27me3 rabbit polyclonal antibody (39155, Active Motif) or anti-mouse IgG as negative control (115-001-003 ...
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bioRxiv - Immunology 2021Quote: ... for 30 minutes at 37°C followed by 2 μl proteinase K (Active Motif, 0.2 mg/ml) at 65°C for 2 hours ...
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bioRxiv - Cancer Biology 2023Quote: ... 5 μg of antibodies were used along with 2 μg of Spike-in antibodies (Active Motif, #61686) were bound to 50 μl of Dynabeads Protein G (Thermo Fisher ...
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bioRxiv - Microbiology 2022Quote: ... Membranes were blocked in 5% milk in PBS-T for 1 h at room temperature and incubated overnight at 4°C with primary antibodies against Lamin AC (Active Motif 39287), Lamin B1 (Abcam ab16048) ...
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bioRxiv - Neuroscience 2019Quote: ... The membrane was incubated with the following primary antibodies diluted in 5% nonfat dry milk/TBS-T overnight at 4°C: rabbit polyclonal anti-5-hydroxymethylcytosine antibody (Active Motif, 39769) at 1:5000 and mouse monoclonal anti-5-methylcytosine antibody (Active Motif ...
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bioRxiv - Molecular Biology 2020Quote: ... 5ug antibodies were added to the supernatant and incubated overnight at 4°C with gentle rotation (CTCF, ab70303; RAD21, ab992; H3K4me3, Millipore, 04-745; H3K27ac, Active Motif,39685.). Chromatin was pulled down by protein G Sepharose beads (#17061801 ...
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bioRxiv - Genetics 2020Quote: ... Each ChIP used 2μg sheared chromatin and was incubated overnight (16 hours) at 4°C with either 5μg of anti-H3K9ac (39137, Active Motif, Carlsbad, CA), or 5μg of anti-H3K27ac (39133 ...
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bioRxiv - Genetics 2019Quote: ... Binding reactions for super shift assays were performed for 30 min at 4-8°C using the Binding Buffer B-1 (Active Motif 37480) together with Stabilizing Solution D (Active Motif 37488 ...
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bioRxiv - Genetics 2019Quote: ... Binding reactions with 3 × 105 cpm of labeled probe was performed for 20 min at 4°-8°C using Binding Buffer C-1 (Active Motif 37484) together with Stabilizing Solution D (Active Motif 37488) ...
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bioRxiv - Developmental Biology 2023Quote: ... and chromatin was incubated for 2h at 4°C with 5 μg of anti-H3K27ac antibody (Active Motif, Cat# 39133, Lot 01613007). Immuno-complexes were sequentially washed ...
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bioRxiv - Cell Biology 2024Quote: ... The H3K27Ac ChIP reactions were conducted with 10 μg of tissue chromatin and 4 μg of H3K27Ac antibody (Active Motif, cat # 39133). The H3K4me1 ChIP reactions were carried out by using 10 ug of tissue chromatin and 4 ul of H3K4me1 antibody (Active Motif ...
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bioRxiv - Molecular Biology 2021Quote: ... Coupled antibody-Dynabeads were prepared by adding Dynabeads protein A/G and 2 μg antibody [H3K9ac (Active Motif) or H3K27ac (Abcam)] to 100 μl ice-cold dilution buffer and rotating the tubes end-over-end at 4°C for 1 hour ...
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bioRxiv - Molecular Biology 2020Quote: ... 2% of the mixture was set aside as input and 20ng of spike-in chromatin (Active Motif, #53083) was added to the rest ...
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bioRxiv - Systems Biology 2023Quote: ... 2 μg of spikein antibody and 20 ng of spike-in chromatin (Active Motif 61686 and 53083, respectively) were added to chromatin prepared from 2.5×106 MV411 cells 72 hours after RNP-mediated TF knockout ...
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bioRxiv - Genetics 2022Quote: ... Around 20 μg or 10 μg of chromatin were incubated overnight at 4°C with 10 μg H3.X/Y or 2.5 μg H3K9me3 antibody respectively (Active Motif-61161, Abcam-ab8898). Chromatinantibody extracted with incubated with protein G Dynal beads (Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2023Quote: ... All incubations with primary antibodies were performed for either 1h at room temperature or overnight at 4°C with the following antibodies for H3K9me3 (Active Motif, 1:1000), DAXX (Santa Cruz ...
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bioRxiv - Developmental Biology 2023Quote: ... Human induced pluripotent stem cell derived cardiomyocytes (iPSC-CMs) from four HLHS patients were fixed in 4% paraformaldehyde and stained with antibody against 5-methylcytosine (Active Motif, RRID: AB_2687950)31 ...
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bioRxiv - Cell Biology 2024Quote: ... The H3K4me1 ChIP reactions were carried out by using 10 ug of tissue chromatin and 4 ul of H3K4me1 antibody (Active Motif, cat # 39297). Libraries were prepared by a custom Illumina library with the standard Illumina PE adaptors (Bentley ...
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bioRxiv - Genomics 2021Quote: ... 2 μg of spike-in antibody and 20 ng of spike-in chromatin (Active Motif 61686 and 53083, respectively) were added to chromatin prepared from 2.5×106 MV411 cells 72 hours after RNP-mediated TF knockout ...
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bioRxiv - Genomics 2021Quote: ... 2 μg of spike-in antibody and 20 ng of spike-in chromatin (Active Motif 61686 and 53083, respectively) were added to chromatin prepared from 2.5×106 MV411 cells 72 hours after RNP-mediated TF knockout ...
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bioRxiv - Genomics 2022Quote: ... aliquots of ∼500,000 cells were added to 20 µl Dynabeads Protein A with 2 µg anti-H3K27ac (Active Motif) and incubated with slow rotation at 4°C overnight ...
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bioRxiv - Genomics 2019Quote: ... and 1x protease inhibitor) before being resuspended in 50 μL of Buffer 2 containing 0.5 μL antibody (H3K27ac from Active Motif, 39122 ...
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bioRxiv - Molecular Biology 2021Quote: ... Total RNA samples were subjected to immunoprecipitation with m6A specific antibody (m6A-1, Active Motif, #91261; m6A-2, Abcam, #ab151230) as previously described5 ...
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bioRxiv - Immunology 2023Quote: Approximately 2 million BMDCs were processed according to the ChIP-IT Express Enzymatic Shearing and ChIP protocol (Active Motif, #53009) as previously described75 ...
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bioRxiv - Genomics 2020Quote: ... were raised against KLH-conjugated dinucleosides at the Technology Development Laboratory (Babraham Bioscience Technologies Ltd, Cambridge, UK) (anti-mCA antibody clone 2C8H8A6 currently available from Active Motif, Cat. No 61783/4). Alexa Fluor secondary antibodies were purchased from Life Technologies ...