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Citations for Active Motif :
1 - 50 of 353 citations for 6 Oxa 7 azatricyclo 3.3.0.02 4 oct 7 ene 8 1 methylethyl 9CI since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genetics 2019Quote: ... Binding reactions for super shift assays were performed for 30 min at 4-8°C using the Binding Buffer B-1 (Active Motif 37480) together with Stabilizing Solution D (Active Motif 37488 ...
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bioRxiv - Genetics 2019Quote: ... Binding reactions with 3 × 105 cpm of labeled probe was performed for 20 min at 4°-8°C using Binding Buffer C-1 (Active Motif 37484) together with Stabilizing Solution D (Active Motif 37488) ...
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bioRxiv - Molecular Biology 2022Quote: ... and incubated with 4-8 μg primary target antibody and 2 μg spike-in antibody (Active Motif, #61686) overnight at 4°C ...
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bioRxiv - Bioengineering 2019Quote: ... for 4-6 hours at room temperature and incubated in MAXbind Staining Medium (Active Motif, catalog no. 15251) containing primary antibodies at a concentration of 10 μg/mL overnight at 4□C ...
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bioRxiv - Immunology 2021Quote: ... and 6 μl 5M NaCL (Active Motif) for 30 minutes at 37°C followed by 2 μl proteinase K (Active Motif ...
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bioRxiv - Cancer Biology 2020Quote: ... The membranes were blocked in 5 % milk/TBST for 1 h followed by primary antibody blotting at 4 °C overnight incubation – 1: 2000 rabbit anti-hmC (cat# 39069, Active Motif). The next day the blots were washed with TBST followed by incubation with secondary 1:2000 anti-rabbit-HRP for 1 h ...
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bioRxiv - Molecular Biology 2023Quote: ... Chromatin was immunoprecipitated using 8 μg anti-H3K27ac (Catalogue No: 39133; Active Motif), 10 μL anti-BRD4 (Bethyl Laboratories ...
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bioRxiv - Molecular Biology 2022Quote: ... After an overnight incubation at 4°C with the primary antibodies (anti-5mC, Eurogentec, ref BI-MECY-0100, 1/500; anti-5hmC, Active Motif, ref 39569, 1/500), and a 1-hour incubation at room temperature with secondary antibodies (Dylight 488 anti-mouse ...
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bioRxiv - Molecular Biology 2023Quote: ... 6 μl of antibody (anti-H3K27Me3, Active Motif 39155, RRID: AB_2561020) was incubated with 100 μl of Dynabeads Protein G (Invitrogen 10004D ...
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bioRxiv - Neuroscience 2020Quote: ... then placed on rotator and incubated overnight at 4°C with 25 uL of Protein G beads and 4 uL (1 mg/mL) anti-RNA polymerase II (102660; Active Motif). Immune complexes were sequentially washed according to kit instructions ...
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bioRxiv - Molecular Biology 2019Quote: ... Isolated cells were picked in 1 μl of 1× PBS and transferred into 4.4 μl of lysing buffer containing 4 μl mTRAP™ Lysis Buffer (Active Motif) and 0.4 μl (10 ng ...
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bioRxiv - Immunology 2019Quote: ... trimethylated H3 lysine 4 (Active Motif 39159), and anti-glutathione-S-transferase (GST ...
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bioRxiv - Cancer Biology 2022Quote: ... 4 µg H3K27ac antibody (Active Motif, 39133) was coupled to 40 µl Dynabeads M280 Anti Rabbit Magnetic beads (Thermo Fisher ...
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bioRxiv - Cancer Biology 2021Quote: ... Chromatin corresponding to 8 μg of DNA was incubated with anti-CTCF (Active Motif; Cat#: 61311) antibody overnight at 4 °C ...
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bioRxiv - Microbiology 2022Quote: ... Membranes were blocked in 5% milk in PBS-T for 1 h at room temperature and incubated overnight at 4°C with primary antibodies against Lamin AC (Active Motif 39287), Lamin B1 (Abcam ab16048) ...
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bioRxiv - Molecular Biology 2023Quote: ... All incubations with primary antibodies were performed for either 1h at room temperature or overnight at 4°C with the following antibodies for H3K9me3 (Active Motif, 1:1000), DAXX (Santa Cruz ...
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bioRxiv - Genomics 2023Quote: ... were pre-coated with antibodies for 4 hours at 4°C and the following antibodies were used: H3K27ac (39685 – Active Motif); H3K9ac (C5B11-9549 – Cell Signalling Technology) ...
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bioRxiv - Developmental Biology 2020Quote: ... Beads were then resuspended in Blocking buffer and coated with antibodies for 4 h at 4°C (H4K20me1 [0.5 μg/IP], Active Motif cat. 39727). Once coated beads were magnet-separated and resuspended in 1 mL of lysate ...
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bioRxiv - Cancer Biology 2019Quote: ... or 4 μg of H3K27Ac antibody (Active Motif #39133). Libraries were prepared by Active Motif and sequenced on a NextSeq 500 to a depth of ∼50 M reads (Jefferson University Genomics Laboratory ...
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bioRxiv - Neuroscience 2021Quote: ... The following primary antibodies were diluted in blocking buffer and applied to cells overnight at 4°C to label key histone modifications associated with epigenetic regulation: rabbit H3K9me3 (1:500; Active Motif, 39765; Carlsbad, CA, USA); mouse H3K4me1 (1:100 ...
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bioRxiv - Genomics 2021Quote: ... The DNA was denatured for 10 min at 95°C and immunoprecipitated overnight at 4°C with 4 μL of 5hmC monoclonal antibody (Active Motif Cat No 55010). To allow selective enrichment of immune-captured DNA fragments ...
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bioRxiv - Neuroscience 2021Quote: ... Cells were washed once with ice cold PBS and lysed in 130μL Sonication Buffer (10mM Tris-HCl pH 8, 0.25% SDS, 2mM EDTA, 1x protease inhibitor cocktail (Active Motif # 37490), 1mM PMSF) ...
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bioRxiv - Cancer Biology 2019Quote: ... overnight at 4 °C or H3K27ac (Active motif; Catalog no: 39133). The purified ChiP DNA was used to generate sequencing libraries using Hapa Hyper prep kit from Kapa Biosystems ...
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bioRxiv - Molecular Biology 2023Quote: ... Histone deacetylases were inhibited with Romidepsin (4 nM; Active Motif, # 14083) or Panobinostat (20 nM ...
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bioRxiv - Molecular Biology 2024Quote: ... Antibody (4 μl per sample) against H3K27ac (Active Motif, cat# 39133) was conjugated to magnetic beads (Sera-Mag ...
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bioRxiv - Biophysics 2021Quote: ... Supplementary Figure 12 and Supplementary Movie 8 show data of a 2% formaldehyde fixed C127 cell immune-labelled with rabbit anti-histone H3K4me3 primary antibodies (Active Motif) and goat-anti rabbit Alexa Fluor 488 secondary antibodies (ThermoFisher) ...
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bioRxiv - Genetics 2023Quote: ... chromatin was sonicated for 8 minutes in Buffer 3 (20 s on, 30 s off, 25% amplitude) using an EpiShear probe sonicator (Active Motif).
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bioRxiv - Cancer Biology 2019Quote: ... Immunoprecipitations were performed using 30 μg of cell line chromatin and 6 μg of N-Myc antibody (Active Motif # 61185) or 4 μg of H3K27Ac antibody (Active Motif #39133) ...
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bioRxiv - Molecular Biology 2021Quote: ... samples were incubated with 4 µg of Pol II antibody (Active Motif, 4H8) at 4°C overnight ...
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bioRxiv - Neuroscience 2023Quote: ... they were incubated overnight at 4°C with the primary antibody (5mC, Active Motif, cat.no ...
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bioRxiv - Systems Biology 2020Quote: ... Extracts were incubated overnight at 4°C with 5 μg of anti-H3K27ac (Active Motif) prebound to protein G Dynabeads (Thermo Scientific ...
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bioRxiv - Cell Biology 2020Quote: ... Primary antibodies (Histone H3 – Abcam ab1791, H3k27me3 – Active Motif – 39155 and TLR-4 -Abcam 76B357.1) were then diluted 1:1000 in blocking buffer and were incubated on a rocker overnight at 4C ...
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bioRxiv - Cancer Biology 2022Quote: ... and rotated at 4°C overnight with 2-5 ug of antibody (H3K27ac, Active Motif Cat #39133 ...
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bioRxiv - Cancer Biology 2023Quote: ... and rotation at 4°C overnight with 2 ug of antibody (H3K27ac, #39133, Active Motif). Transcription factor ChIP-Seq was performed as previously described11 with 2-5 ug of antibody was used per immunoprecipitation (POU2F2 ...
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bioRxiv - Cell Biology 2019Quote: Isolation of nuclear protein extracts from ATI-like cells after 6 days of culture was performed with a Nuclear Extract Kit according to the manufacturer’s instructions (Active Motif, 40010, La Hulpe, Belgium) as described (Hofmann et al. ...
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bioRxiv - Genomics 2020Quote: ... and immunoprecipitation was performed using 4 μg of the appropriate antibody (anti-CTCF: 61311, Active Motif; anti-RAD21 ...
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bioRxiv - Systems Biology 2019Quote: ... Permeabilized cells were incubated overnight at 4°C with 5ug of anti-H3K27me3 (Active Motif 39156) and then washed before incubating with protein A-MNase fusion protein (a gift from S ...
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bioRxiv - Cancer Biology 2021Quote: ... and genomic DNA regions of interest isolated using 4 μg of H3K27Ac antibody (Active Motif, cat# 39133). Complexes were washed ...
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bioRxiv - Molecular Biology 2020Quote: ... 4 - 10 µg of target antibodies and 2μg of spike-in antibody (anti-H2Av, Active Motif, #61686) were added and incubated on a rotator at 4°C overnight ...
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bioRxiv - Genomics 2020Quote: ... 5 - 33 μg of sonicated chromatin was incubated with 4 μg ChIP-validated CTCF antibody (Active Motif) overnight on an end-to-end rotator at 4°C ...
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bioRxiv - Cancer Biology 2024Quote: ... anti-H3.1/2 (Active Motif #61629; 1/250, 1/1000 and 1/4000) and anti-PTM (EMD Millipore #04-263 ...
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bioRxiv - Biophysics 2024Quote: ... anti-H3.1/2 (Active Motif #61629; 1/250, 1/1000 and 1/4000) and anti-PTM (EMD Millipore #04-263 ...
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bioRxiv - Immunology 2020Quote: ... Lysates were incubated overnight at 4°C with 5 μg antibody against RNA Pol II (39097, Active Motif), RelA (A301-824A ...
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bioRxiv - Immunology 2021Quote: ... 1:1000 (Active Motif #61647, aa 1-19); Histone H4 C-terminal ...
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bioRxiv - Cancer Biology 2019Quote: ... Genomic DNA regions of interest were isolated using 4 ug of antibody against Histone H3K27me3 (clone: 39155, Active Motif) Complexes were washed ...
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bioRxiv - Biochemistry 2019Quote: ... at 4°C with Active Motif enzymatic shearing cocktail in 100 µl Complete Digestion Buffer (both from Active Motif), and centrifuged for 10 min ...
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bioRxiv - Genomics 2019Quote: ... 5 million cells per replicate were incubated with 5 ug (Histone) or 7.5 ug (Oct4) antibody overnight at 4’C (antibodies: H3K9me3: abcam AB8898; H3K27Ac: Active Motif 39133 ...
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bioRxiv - Cancer Biology 2020Quote: ... Genomic DNA regions of interest were isolated using 4 ug of antibody against H3K27Ac (Active Motif, cat# 39133, Lot# 28518012). Complexes were washed ...
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bioRxiv - Cancer Biology 2019Quote: ... Genomic DNA regions of interest were isolated using 4 µg of antibody against H3K27Ac (Active Motif, cat# 39133, Lot# 01518010). Complexes were washed ...
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bioRxiv - Neuroscience 2023Quote: ... Drosophila chromatin (Active Motif #53083; 1 ng per 1 μg chromatin) was added to the samples ...