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Citations for Active Motif :
1 - 50 of 389 citations for 6β Fluoro 21 hydroxypregna 1 4 9 11 16 tetraene 3 20 dione 21 acetate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2022Quote: ... with biotinylated 21-mer H3K4me2 peptide (Active Motif, 81041) at 0.5µg/ml of 2h ...
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bioRxiv - Molecular Biology 2023Quote: One μg of histone H3 peptides with or without H3K4me2 (a.a. 1-21, Active Motif) were incubated with 1 μg of purified GST-PHF21A protein fragments in binding buffer (50 mM Tris/Cl at pH 7.5 ...
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bioRxiv - Genetics 2019Quote: ... Binding reactions with 3 × 105 cpm of labeled probe was performed for 20 min at 4°-8°C using Binding Buffer C-1 (Active Motif 37484) together with Stabilizing Solution D (Active Motif 37488) ...
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bioRxiv - Developmental Biology 2020Quote: ... H3K27me2/3 (Active motif, 39535, 1:100) and EZH2(BD43 clone ...
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bioRxiv - Genetics 2020Quote: ... Each ChIP used 2μg sheared chromatin and was incubated overnight (16 hours) at 4°C with either 5μg of anti-H3K9ac (39137, Active Motif, Carlsbad, CA), or 5μg of anti-H3K27ac (39133 ...
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bioRxiv - Genomics 2021Quote: ... ChIP enrichment was performed by incubation for 16 h at 4°C with the following antibodies: 2 µg H3K27ac antibody (Active Motif #39133), 4 µg H3K4me1 antibody (Millipore 07-436) ...
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bioRxiv - Developmental Biology 2020Quote: ... pre-cleared for 2 h followed by immunoprecipitation with primary antibodies for 12-16 hours at 4°C (H3K27ac, Active motif 39133; H3K27me3, CST 9733s). 5% of samples were used as input DNA ...
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bioRxiv - Neuroscience 2024Quote: ... Immunoprecipitation was performed with 9 μg of anti-H4K8ac (Active Motif, 61103) per ChIP sample after preclearing chromatin with protein A-Dynabeads ...
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bioRxiv - Genetics 2023Quote: ... chromatin was sonicated for 8 minutes in Buffer 3 (20 s on, 30 s off, 25% amplitude) using an EpiShear probe sonicator (Active Motif).
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bioRxiv - Bioengineering 2023Quote: ... and mouse anti-MEIS 1/2/3 antibody (1:200, clone 9.2.7, 39796, Active Motif, RRID:AB_2750570). Secondary antibodies (raised in donkey ...
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bioRxiv - Neuroscience 2022Quote: ... ChIP reactions were carried out overnight at 4 °C with 3 µg of sheared chromatin and 10 μg of anti-H3K27me3 rabbit polyclonal antibody (39155, Active Motif) or anti-mouse IgG as negative control (115-001-003 ...
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bioRxiv - Cancer Biology 2023Quote: ... After 1 hour blocking (3% donkey serum, 1% BSA, 0.3% Triton X-100, PBS) the H3.3K27M antibody (1:500, 61803, Active Motif) diluted in a blocking solution was added and incubated for 1 hour ...
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Identification of a PRC2 accessory subunit required for subtelomeric H3K27 methylation in NeurosporabioRxiv - Molecular Biology 2020Quote: H3K27me2/3 ChIP using anti-H3K27me2/3 antibody (Active Motif, 39536) was performed as previously described (12 ...
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bioRxiv - Genetics 2021Quote: H3K27me2/3 ChIP using α-H3K27me2/3 antibody (Active Motif, 39536), which recognizes di- or trimethylated H3K27 ...
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bioRxiv - Genomics 2023Quote: ... The membrane was then blocked in 5% skimmed milk in TBS containing 0.1% Tween 20 (TBST) for 1 h at room temperature followed by incubation with 1:10,000 dilution of 5hmC antibody (Active Motif, 39069) overnight at 4°C ...
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bioRxiv - Molecular Biology 2022Quote: ... chromatin from 1 x 107 cells were mixed with 20 ng spike-in chromatin (Active Motif, #53083) and incubated with 4-8 μg primary target antibody and 2 μg spike-in antibody (Active Motif ...
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bioRxiv - Cancer Biology 2020Quote: ... The membranes were blocked in 5 % milk/TBST for 1 h followed by primary antibody blotting at 4 °C overnight incubation – 1: 2000 rabbit anti-hmC (cat# 39069, Active Motif). The next day the blots were washed with TBST followed by incubation with secondary 1:2000 anti-rabbit-HRP for 1 h ...
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bioRxiv - Developmental Biology 2019Quote: ... MEIS1/2/3 (Active Motif 39796) and LTL (Vectorshield FL-1321) ...
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bioRxiv - Cell Biology 2022Quote: ... Histone 3 (H3) (Active Motif 39763), Histone H3K4me3 (Active Motif 39160) ...
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bioRxiv - Cancer Biology 2024Quote: Purified core histone extracts prepared as described above were quantified for histone lysine trimethylation at lysine 9 and lysine 27 following ELISA protocols from Active motif (Catalogue # 53109 and 53106 ...
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bioRxiv - Molecular Biology 2022Quote: ... After an overnight incubation at 4°C with the primary antibodies (anti-5mC, Eurogentec, ref BI-MECY-0100, 1/500; anti-5hmC, Active Motif, ref 39569, 1/500), and a 1-hour incubation at room temperature with secondary antibodies (Dylight 488 anti-mouse ...
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bioRxiv - Cancer Biology 2020Quote: ... Supershift analysis was carried out by antibodies to RUNX 1 or RUNX 3 (Active Motif cat# 39300 and 39301, respectively). The gel was exposed to X ray film and/or phosphorimaging screens ...
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bioRxiv - Biophysics 2022Quote: ... and 3 μM Sortase A5 (Active Motif) in 20 mM Tris ...
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bioRxiv - Cancer Biology 2022Quote: ... (Histone 3 (Active Motif, cat no. 39763); GAPDH (Santa Cruz Biotechnology ...
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bioRxiv - Biophysics 2022Quote: H3K27ac (Active Motif, 39133 Lot 06921014 – Figure 3), biological replicates from Supplementary Figure SI-Fig ...
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bioRxiv - Molecular Biology 2019Quote: ... and coupled to one of the following antibodies in the same buffer for 1 hr at room temperature with rotation: 3 μl H3 (Active motif 39763), 5 μl H3K4me3 (Millipore 04-745) ...
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bioRxiv - Cancer Biology 2020Quote: ... 20 ng Spike-in Drosophila chromatin (Active Motif, 53083) was supplemented to chromatin preps for Spike-in normalisation ...
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bioRxiv - Cancer Biology 2022Quote: ... 20 ng Spike-in Drosophila chromatin (Active Motif, 53083) was supplemented to chromatin preps for Spike-in normalisation ...
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bioRxiv - Neuroscience 2020Quote: ... then placed on rotator and incubated overnight at 4°C with 25 uL of Protein G beads and 4 uL (1 mg/mL) anti-RNA polymerase II (102660; Active Motif). Immune complexes were sequentially washed according to kit instructions ...
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bioRxiv - Molecular Biology 2019Quote: ... Isolated cells were picked in 1 μl of 1× PBS and transferred into 4.4 μl of lysing buffer containing 4 μl mTRAP™ Lysis Buffer (Active Motif) and 0.4 μl (10 ng ...
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bioRxiv - Immunology 2019Quote: ... trimethylated H3 lysine 4 (Active Motif 39159), and anti-glutathione-S-transferase (GST ...
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bioRxiv - Cancer Biology 2022Quote: ... 4 µg H3K27ac antibody (Active Motif, 39133) was coupled to 40 µl Dynabeads M280 Anti Rabbit Magnetic beads (Thermo Fisher ...
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bioRxiv - Cell Biology 2023Quote: 3’UTR reporter vectors were purchased from Active Motif, along with the miR-4787 mimic ...
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bioRxiv - Genomics 2023Quote: ... Human Negative Control Primer Set 3 (Active Motif, #71023). For analysis ...
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bioRxiv - Cancer Biology 2024Quote: ... MARK3 and 14-3-3ε were purchased (Active Motif, 81209 ...
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bioRxiv - Microbiology 2022Quote: ... Membranes were blocked in 5% milk in PBS-T for 1 h at room temperature and incubated overnight at 4°C with primary antibodies against Lamin AC (Active Motif 39287), Lamin B1 (Abcam ab16048) ...
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bioRxiv - Genetics 2019Quote: ... Binding reactions for super shift assays were performed for 30 min at 4-8°C using the Binding Buffer B-1 (Active Motif 37480) together with Stabilizing Solution D (Active Motif 37488 ...
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bioRxiv - Genetics 2020Quote: ... 3 μl of H3K27ac monoclonal antibody (Cat #39685, Active Motif) was added to each sample and rotated overnight at 4°C ...
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bioRxiv - Cell Biology 2020Quote: RelA and IRF-3 transcription factor activation assays (Active Motif) were carried out using the same protocol with the only difference being the 96-well plates and the antibodies used ...
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bioRxiv - Molecular Biology 2023Quote: ... All incubations with primary antibodies were performed for either 1h at room temperature or overnight at 4°C with the following antibodies for H3K9me3 (Active Motif, 1:1000), DAXX (Santa Cruz ...
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bioRxiv - Genomics 2023Quote: ... were pre-coated with antibodies for 4 hours at 4°C and the following antibodies were used: H3K27ac (39685 – Active Motif); H3K9ac (C5B11-9549 – Cell Signalling Technology) ...
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bioRxiv - Cancer Biology 2020Quote: ... Following addition of 20 ng spike-in chromatin (Active Motif 61686) and 2 μg spike-in antibody (Active Motif 53083) ...
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bioRxiv - Developmental Biology 2020Quote: ... Beads were then resuspended in Blocking buffer and coated with antibodies for 4 h at 4°C (H4K20me1 [0.5 μg/IP], Active Motif cat. 39727). Once coated beads were magnet-separated and resuspended in 1 mL of lysate ...
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bioRxiv - Cancer Biology 2019Quote: ... or 4 μg of H3K27Ac antibody (Active Motif #39133). Libraries were prepared by Active Motif and sequenced on a NextSeq 500 to a depth of ∼50 M reads (Jefferson University Genomics Laboratory ...
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bioRxiv - Molecular Biology 2019Quote: ... -H3K27 di-and tri-methyl (H3K27me2/3, Active Motif, cat # 39538), -nuclear pore glycoprotein p62 (NUP62 ...
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bioRxiv - Cell Biology 2022Quote: ... 3 µl of anti-H3K4me3 antibodies (Active Motif, cat. No 39159) were added to the sonicated ChIP reaction and rotated vertically at 4° C overnight ...
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bioRxiv - Neuroscience 2021Quote: ... The following primary antibodies were diluted in blocking buffer and applied to cells overnight at 4°C to label key histone modifications associated with epigenetic regulation: rabbit H3K9me3 (1:500; Active Motif, 39765; Carlsbad, CA, USA); mouse H3K4me1 (1:100 ...
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bioRxiv - Genomics 2021Quote: ... The DNA was denatured for 10 min at 95°C and immunoprecipitated overnight at 4°C with 4 μL of 5hmC monoclonal antibody (Active Motif Cat No 55010). To allow selective enrichment of immune-captured DNA fragments ...
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bioRxiv - Cancer Biology 2020Quote: SDHD 3’UTR LightSwitch Luciferase Reporter Assay was purchased from Active Motif. Cells were co-transfected with 30 nM miRNA and 100 ng reporter using Lipofectamine 3000 in a 96-well plate ...
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bioRxiv - Genomics 2021Quote: ... Chromatin was sonicated for 3 minutes with a probe sonicator (Active Motif). Finally ...