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Citations for Active Motif :
401 - 428 of 428 citations for Indusatumab Anti GUCY2C since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2023Quote: ... The western blots of the chromatin-associated proteins were performed with anti-RNA pol II CTD phospho Tyr1 (Active Motif), anti-Rpb1 (manufactured in our laboratory ...
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bioRxiv - Cancer Biology 2024Quote: ... The membranes were then blocked in 5% milk/TBST for 1 h followed by primary antibody incubation at 4 °C overnight at dilution of 1:10,000 rabbit anti hmC (catalog no. 39069, Active Motif). The blots were washed with TBST followed by incubation with secondary 1:3,000 anti-rabbit-HRP for 5-hmC for 1 h ...
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bioRxiv - Genetics 2021Quote: ... Coverslips were removed and slides were washed in PBS with 0.1% Triton X-100 (PBST) (3X 10 minutes each) and subsequently incubated with primary rabbit anti-H3K9me3 (Active Motif #39765) antibodies (at 1:500 dilution ...
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bioRxiv - Biophysics 2021Quote: ... Supplementary Figure 12 and Supplementary Movie 8 show data of a 2% formaldehyde fixed C127 cell immune-labelled with rabbit anti-histone H3K4me3 primary antibodies (Active Motif) and goat-anti rabbit Alexa Fluor 488 secondary antibodies (ThermoFisher) ...
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bioRxiv - Neuroscience 2021Quote: ... The sonicated lysate was added to 20 µl Dynabeads Protein A with 1.5 ug anti-H3K27ac (Active Motif, #39685 Mouse Monoclonal) and incubated with slow rotation at 4°C overnight ...
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bioRxiv - Neuroscience 2020Quote: ... then placed on rotator and incubated overnight at 4°C with 25 uL of Protein G beads and 4 uL (1 mg/mL) anti-RNA polymerase II (102660; Active Motif). Immune complexes were sequentially washed according to kit instructions ...
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bioRxiv - Immunology 2022Quote: ... 1 × 105 BM pDCs sorted from Hdac3fl/fl control or Hdac3fl/fl CreERT2 littermates were bound to beads and were subjected for immunoprecipitation with primary anti-H3K27ac antibody (Active Motif) at 4°C overnight ...
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bioRxiv - Genetics 2020Quote: ... Chr13.1-cl and Chr13.2-cl KO ES cells was determined by native ChIP-seq and anti-H3K9me3 serum (Active Motif, #39161) as described previously (45) ...
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bioRxiv - Genomics 2020Quote: ... The following primary antibodies were purchased and used for immunofluorescence and CUT&RUN27,28 followed by sequencing: Rabbit anti-H3K4me3 (Active motif, 39159), Rabbit anti-H3K27me3 (Active Motif ...
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bioRxiv - Molecular Biology 2020Quote: ... Beads were washed three times with PBS and bound proteins were recovered by adding Laemmli buffer and then analysed by immunoblotting with anti-DNMT1 antibody (Active Motif).
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bioRxiv - Cancer Biology 2020Quote: ... The membranes were blocked in 5 % milk/TBST for 1 h followed by primary antibody blotting at 4 °C overnight incubation – 1: 2000 rabbit anti-hmC (cat# 39069, Active Motif). The next day the blots were washed with TBST followed by incubation with secondary 1:2000 anti-rabbit-HRP for 1 h ...
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bioRxiv - Neuroscience 2022Quote: ... ChIP reactions were carried out overnight at 4 °C with 3 µg of sheared chromatin and 10 μg of anti-H3K27me3 rabbit polyclonal antibody (39155, Active Motif) or anti-mouse IgG as negative control (115-001-003 ...
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bioRxiv - Molecular Biology 2023Quote: ... the cells were incubated with anti-5mC antibody (1:200 diluted in 0.5% goat serum + PBST, 39649, Active Motif, CA, USA) at 4 °C overnight and then washed three times with PBST for 5 min each ...
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bioRxiv - Immunology 2024Quote: ... Cells bound to the beads were resuspended in Dig-Wash Buffer and incubated with a 1:50 dilution of primary antibodies (rabbit anti-H3K4me3, Active Motif,39016 ...
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bioRxiv - Genomics 2024Quote: For anti-CENP-A CUT&Tag library preparation was used the CUT&Tag-IT kit from Active Motif (Cat. No. 53160). Each experiment was performed for 500,000 fresh cells ...
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bioRxiv - Cell Biology 2024Quote: ... and incubated with a 1:500 dilution of the appropriate primary antibody for 1 h at room temperature (anti-CTCF, Active Motif 61331 ...
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bioRxiv - Cancer Biology 2024Quote: ... were denatured by heating to 98C for 10min and chilled on ice for 10min and incubated overnight with anti-5hmC antibody (4μl, Active Motif #39791) in 20mM Na-Phosphate pH 7.0 ...
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bioRxiv - Cancer Biology 2021Quote: ... Relative 5hmC levels were quantified blotting 500 ng of gDNA on a nitrocellulose membrane with an anti-5hmC antibody (Active Motif, 39769) diluted 1/10000 followed by an anti-rabbit antibody coupled to horseradish peroxydase (Dutscher NA934 ...
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bioRxiv - Genetics 2020Quote: ... Each ChIP used 2μg sheared chromatin and was incubated overnight (16 hours) at 4°C with either 5μg of anti-H3K9ac (39137, Active Motif, Carlsbad, CA), or 5μg of anti-H3K27ac (39133 ...
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bioRxiv - Molecular Biology 2022Quote: Cleavage under targets and tagmentation (CUT&Tag [35]) was performed for three biological replicates per experimental condition using the CUT&Tag-IT Assay Kit (Anti-Rabbit) (Active Motif, 53160) according to the manufacturer’s instructions with some modifications ...
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bioRxiv - Developmental Biology 2023Quote: ... and chromatin was incubated for 2h at 4°C with 5 μg of anti-H3K27ac antibody (Active Motif, Cat# 39133, Lot 01613007). Immuno-complexes were sequentially washed ...
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bioRxiv - Microbiology 2023Quote: ... Beads were resuspended in 100 μl of IP/wash buffer and coated with 5 μg of rabbit anti-m6A antibody (Active motif, 61495) for 45 min at room temperature with rotation ...
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bioRxiv - Cell Biology 2024Quote: ... Pre-cleared chromatin samples were incubated overnight at 4°C on a rotator with histone modification antibodies–either anti-H3K4me3 (Active Motif, 39159), anti-H3K27ac (Abcam ...
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bioRxiv - Molecular Biology 2021Quote: ... and carried out from OSCs according to Fabry and colleagues (Fabry et al., 2019) using commercially available rabbit polyclonal anti-H3K9me3 (Active Motif, cat # 39161) and rabbit polyclonal anti-H3K4me2 (Merck Millipore ...
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bioRxiv - Microbiology 2022Quote: ... Parasite pellets were then processed as per the manufacturer’s protocol (CUT&Tag-IT™ Assay Kit, Anti-Rabbit, Active motif, Cat no. 53160). Libraries were sequenced by Azenta at PE 2×150 bp.
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bioRxiv - Microbiology 2024Quote: ... The primary antibodies were used at the following dilutions: anti-Cas9 (1:1000 in 5% milk/PBS-T, Active Motif, clone 7A9-3A3); anti-EF1α (1:20,000 in 1% milk/PBS-T ...
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bioRxiv - Immunology 2024Quote: ... cells were incubated overnight with Concanavalin A beads and 1 µl of the primary anti-H3K4me1 antibody per reaction (Active Motif, catalog number 39297) and 1 µl of the primary anti-H3K27ac antibody per reaction (Active Motif ...
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bioRxiv - Genomics 2020Quote: ... were raised against KLH-conjugated dinucleosides at the Technology Development Laboratory (Babraham Bioscience Technologies Ltd, Cambridge, UK) (anti-mCA antibody clone 2C8H8A6 currently available from Active Motif, Cat. No 61783/4). Alexa Fluor secondary antibodies were purchased from Life Technologies ...