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Citations for Active Motif :
401 - 440 of 440 citations for Goat Anti Mouse IgG since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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CENP-C regulates centromere assembly, asymmetry and epigenetic age in Drosophila germline stem cellsbioRxiv - Cell Biology 2020Quote: ... the following antibodies were used: rabbit anti-CENP-A (CID) antibody (Active Motif 39719; 1:1000), sheep anti-CENP-C (Dattoli et al ...
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bioRxiv - Cancer Biology 2021Quote: ... Tissues were stained with anti-acetylated histone H3 antibody (1:250; #39140, Active Motif, CA, USA) overnight at 4°C ...
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bioRxiv - Neuroscience 2023Quote: ... were coated with antibodies (Cell Signaling Technology Rabbit anti-H3K27me3 Cat#9733, RRID:AB_2616029 and Active Motif Rabbit anti-H3K4me3 Cat# 39159 ...
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bioRxiv - Cancer Biology 2023Quote: ... 0.01%SDS and 1.1% Triton x100) and incubated with primary antibodies (anti-H3K27Ac, Active Motif #39133) overnight at 4º C ...
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bioRxiv - Systems Biology 2023Quote: ... Antibodies were used at the following concentrations: Anti H3K27ac 1:1000 (Active Motif Cat No. 39685), and /or Anti H3K27me3 1:1000 (Active Motif Cat No ...
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bioRxiv - Molecular Biology 2020Quote: ... 4 - 10 µg of target antibodies and 2μg of spike-in antibody (anti-H2Av, Active Motif, #61686) were added and incubated on a rotator at 4°C overnight ...
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bioRxiv - Cell Biology 2021Quote: ... Histone modifications were detected with the following primary antibodies: rabbit polyclonal anti-H2AT120ph antibody (Active Motif, 39391) at a 1:10 dilution ...
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bioRxiv - Developmental Biology 2022Quote: ... the following primary antibodies were used: rabbit anti-CENP-A (CID) antibody (Active Motif 39719; 1:500), sheep anti-CENP-C (Dattoli et al. ...
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bioRxiv - Neuroscience 2023Quote: ... and secondary antibody was guinea pig anti-rabbit (Active Motif, 105465 from CUT&TagIT kit, 1:100).
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bioRxiv - Molecular Biology 2021Quote: ... The membrane was probed with anti-bodies specific to 5mC (Active motif # 39649; dilution factor 1:1. 000). To control loading ...
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bioRxiv - Cancer Biology 2021Quote: ... The following primary antibodies were used: anti-FOXA1 (GeneTex, catalog no. GTX100308 and Active motif, catalog no. 39837) and GATA3 (Assay Biotech ...
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bioRxiv - Pathology 2020Quote: ... and FAC-sorted β-cells were used for histone H3K27ac ChIPseq with anti-H3K27ac antibody (Active Motif, 39133). ChIP and ChIPseq were performed as previously described (Kuo et al. ...
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bioRxiv - Developmental Biology 2019Quote: ... Drosophila chromatin and anti-H2Av were used according to manufacturer’s instructions were followed (Active Motif, 53093 and 61686). For immunoprecipitation ...
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Transcription factor LSF facilitiates lysine methylation of α-tubulin by microtubule-associated SET8bioRxiv - Biochemistry 2019Quote: ... 1 mg of total HEK293T cellular extract was incubated with 5 μg of anti-SET8 antibody (Active Motif, 61009), anti-LSF antibody (Millipore,17-10252) ...
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bioRxiv - Genomics 2021Quote: ... Rabbit anti-H3K4me3 (1:100 for bulk profiling or 1:10 for single-cell experiments, Active Motif Cat# 39159), Rabbit anti-H3K36me3 (1:100 for bulk profiling or 1:10 for single-cell experimemts ...
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bioRxiv - Genomics 2022Quote: ... aliquots of ∼500,000 cells were added to 20 µl Dynabeads Protein A with 2 µg anti-H3K27ac (Active Motif) and incubated with slow rotation at 4°C overnight ...
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bioRxiv - Microbiology 2023Quote: ... The membranes were blocked in 5% milk and incubated with anti-CTCF antibody (Active Motif 61311; 1:1,000 dilution), anti-β-actin antibody (Sigma-Aldrich ...
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bioRxiv - Neuroscience 2023Quote: ... Chromatin immunoprecipitation was carried out using multiplexed MOWChIP assay (Zhu et al., 2019) with anti-H3K4me3 (39159, Active Motif) and anti-H3K27ac (39135 ...
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bioRxiv - Genomics 2020Quote: ... and incubated with the appropriate primary antibody for 1 h at room temperature (anti-RNAPII: 1:500, 61086, Active Motif; anti-H3K27ac ...
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bioRxiv - Neuroscience 2021Quote: ... ChIP was carried out using multiplexed MOWChIP-seq with MNase digestion for chromatin fragmentation and anti-H3K27ac (39135, Active Motif) antibody ...
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bioRxiv - Molecular Biology 2023Quote: ... The western blots of the chromatin-associated proteins were performed with anti-RNA pol II CTD phospho Tyr1 (Active Motif), anti-Rpb1 (manufactured in our laboratory ...
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bioRxiv - Genetics 2021Quote: ... Coverslips were removed and slides were washed in PBS with 0.1% Triton X-100 (PBST) (3X 10 minutes each) and subsequently incubated with primary rabbit anti-H3K9me3 (Active Motif #39765) antibodies (at 1:500 dilution ...
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bioRxiv - Biophysics 2021Quote: ... Supplementary Figure 12 and Supplementary Movie 8 show data of a 2% formaldehyde fixed C127 cell immune-labelled with rabbit anti-histone H3K4me3 primary antibodies (Active Motif) and goat-anti rabbit Alexa Fluor 488 secondary antibodies (ThermoFisher) ...
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bioRxiv - Neuroscience 2020Quote: ... then placed on rotator and incubated overnight at 4°C with 25 uL of Protein G beads and 4 uL (1 mg/mL) anti-RNA polymerase II (102660; Active Motif). Immune complexes were sequentially washed according to kit instructions ...
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bioRxiv - Immunology 2022Quote: ... 1 × 105 BM pDCs sorted from Hdac3fl/fl control or Hdac3fl/fl CreERT2 littermates were bound to beads and were subjected for immunoprecipitation with primary anti-H3K27ac antibody (Active Motif) at 4°C overnight ...
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bioRxiv - Genetics 2020Quote: ... Chr13.1-cl and Chr13.2-cl KO ES cells was determined by native ChIP-seq and anti-H3K9me3 serum (Active Motif, #39161) as described previously (45) ...
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bioRxiv - Genomics 2020Quote: ... The following primary antibodies were purchased and used for immunofluorescence and CUT&RUN27,28 followed by sequencing: Rabbit anti-H3K4me3 (Active motif, 39159), Rabbit anti-H3K27me3 (Active Motif ...
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bioRxiv - Molecular Biology 2020Quote: ... Beads were washed three times with PBS and bound proteins were recovered by adding Laemmli buffer and then analysed by immunoblotting with anti-DNMT1 antibody (Active Motif).
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bioRxiv - Cancer Biology 2020Quote: ... The membranes were blocked in 5 % milk/TBST for 1 h followed by primary antibody blotting at 4 °C overnight incubation – 1: 2000 rabbit anti-hmC (cat# 39069, Active Motif). The next day the blots were washed with TBST followed by incubation with secondary 1:2000 anti-rabbit-HRP for 1 h ...
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bioRxiv - Neuroscience 2022Quote: ... ChIP reactions were carried out overnight at 4 °C with 3 µg of sheared chromatin and 10 μg of anti-H3K27me3 rabbit polyclonal antibody (39155, Active Motif) or anti-mouse IgG as negative control (115-001-003 ...
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bioRxiv - Cancer Biology 2021Quote: ... Relative 5hmC levels were quantified blotting 500 ng of gDNA on a nitrocellulose membrane with an anti-5hmC antibody (Active Motif, 39769) diluted 1/10000 followed by an anti-rabbit antibody coupled to horseradish peroxydase (Dutscher NA934 ...
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bioRxiv - Neuroscience 2019Quote: ... The membrane was incubated with the following primary antibodies diluted in 5% nonfat dry milk/TBS-T overnight at 4°C: rabbit polyclonal anti-5-hydroxymethylcytosine antibody (Active Motif, 39769) at 1:5000 and mouse monoclonal anti-5-methylcytosine antibody (Active Motif ...
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bioRxiv - Genetics 2020Quote: ... Each ChIP used 2μg sheared chromatin and was incubated overnight (16 hours) at 4°C with either 5μg of anti-H3K9ac (39137, Active Motif, Carlsbad, CA), or 5μg of anti-H3K27ac (39133 ...
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bioRxiv - Molecular Biology 2022Quote: Cleavage under targets and tagmentation (CUT&Tag [35]) was performed for three biological replicates per experimental condition using the CUT&Tag-IT Assay Kit (Anti-Rabbit) (Active Motif, 53160) according to the manufacturer’s instructions with some modifications ...
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bioRxiv - Developmental Biology 2023Quote: ... and chromatin was incubated for 2h at 4°C with 5 μg of anti-H3K27ac antibody (Active Motif, Cat# 39133, Lot 01613007). Immuno-complexes were sequentially washed ...
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bioRxiv - Microbiology 2023Quote: ... Beads were resuspended in 100 μl of IP/wash buffer and coated with 5 μg of rabbit anti-m6A antibody (Active motif, 61495) for 45 min at room temperature with rotation ...
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bioRxiv - Molecular Biology 2021Quote: ... and carried out from OSCs according to Fabry and colleagues (Fabry et al., 2019) using commercially available rabbit polyclonal anti-H3K9me3 (Active Motif, cat # 39161) and rabbit polyclonal anti-H3K4me2 (Merck Millipore ...
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bioRxiv - Microbiology 2022Quote: ... Parasite pellets were then processed as per the manufacturer’s protocol (CUT&Tag-IT™ Assay Kit, Anti-Rabbit, Active motif, Cat no. 53160). Libraries were sequenced by Azenta at PE 2×150 bp.
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bioRxiv - Microbiology 2024Quote: ... The primary antibodies were used at the following dilutions: anti-Cas9 (1:1000 in 5% milk/PBS-T, Active Motif, clone 7A9-3A3); anti-EF1α (1:20,000 in 1% milk/PBS-T ...
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bioRxiv - Genomics 2020Quote: ... were raised against KLH-conjugated dinucleosides at the Technology Development Laboratory (Babraham Bioscience Technologies Ltd, Cambridge, UK) (anti-mCA antibody clone 2C8H8A6 currently available from Active Motif, Cat. No 61783/4). Alexa Fluor secondary antibodies were purchased from Life Technologies ...