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Citations for Active Motif :
401 - 450 of 475 citations for GA binding protein alpha chain GABPA Antibody since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2022Quote: ... They were then incubated overnight at 4C with the primary antibodies in PBST (Active motif (2AG-6F12-H4) anti-H3K9me3 (1:100) ...
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bioRxiv - Developmental Biology 2024Quote: ... Western blots were performed using the following antibodies: anti-DNMT3A (Active Motif # 39206, anti-pERK1/2 (CST #9101), and anti-histone H3 (Abcam #ab1791).
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bioRxiv - Genomics 2021Quote: ... 2 μg of spike-in antibody and 20 ng of spike-in chromatin (Active Motif 61686 and 53083, respectively) were added to chromatin prepared from 2.5×106 MV411 cells 72 hours after RNP-mediated TF knockout ...
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bioRxiv - Genomics 2021Quote: ... 2 μg of spike-in antibody and 20 ng of spike-in chromatin (Active Motif 61686 and 53083, respectively) were added to chromatin prepared from 2.5×106 MV411 cells 72 hours after RNP-mediated TF knockout ...
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bioRxiv - Cancer Biology 2023Quote: ... the bead/nuclei mixture was incubated overnight with a 1:100 dilution of primary antibody (H3K27ac (39133, Active Motif)) which was followed by secondary antibody (13-1047 ...
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bioRxiv - Cancer Biology 2023Quote: ... After 1 hour blocking (3% donkey serum, 1% BSA, 0.3% Triton X-100, PBS) the H3.3K27M antibody (1:500, 61803, Active Motif) diluted in a blocking solution was added and incubated for 1 hour ...
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bioRxiv - Microbiology 2023Quote: ... The membranes were blocked in 5% milk and incubated with anti-CTCF antibody (Active Motif 61311; 1:1,000 dilution), anti-β-actin antibody (Sigma-Aldrich ...
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bioRxiv - Biochemistry 2024Quote: ... ChIP was carried out overnight at 4°C using IgG control or H4ac Pan-acetylation antibody (Active Motif # 39243) with anti-rabbit Dynabeads blocked with BSA ...
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bioRxiv - Genomics 2020Quote: ... and incubated with the appropriate primary antibody for 1 h at room temperature (anti-RNAPII: 1:500, 61086, Active Motif; anti-H3K27ac ...
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bioRxiv - Cancer Biology 2020Quote: ... Genomic DNA regions of interest were isolated using 4 ug of antibody against H3K27Ac (Active Motif, cat# 39133, Lot# 28518012). Complexes were washed ...
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bioRxiv - Neuroscience 2022Quote: ... 1:50 Dilutions of primary antibodies against the following active and repressive histone modifications were used: H3K4me3 (Active Motif, 39159), H3K27ac (Abcam ...
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bioRxiv - Cancer Biology 2022Quote: ... 1X HALT protease inhibitor cocktail) with 1:100 corresponding antibody (anti-CHD4, Cell Signaling Technology, #12011S; anti-HDAC2, Active motif, #31505 ...
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bioRxiv - Developmental Biology 2022Quote: ... This DNA was then used in immunoprecipitation (IP) reactions using 5-Methylcytosine (5-mC) mouse monoclonal antibody (39649, Active Motif). Immunoprecipitated DNA and input control (pooled DNA that did not go through the IP step ...
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bioRxiv - Molecular Biology 2021Quote: ... Total RNA samples were subjected to immunoprecipitation with m6A specific antibody (m6A-1, Active Motif, #91261; m6A-2, Abcam, #ab151230) as previously described5 ...
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bioRxiv - Genomics 2021Quote: ... 1:50 Dilutions of primary antibodies against the following active and repressive histone modifications were used: H3K4me3 (Active Motif, 39159), H3K27ac (Abcam ...
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bioRxiv - Genetics 2020Quote: ... containing primary antibody (rabbit anti-H3K9me3, Abcam ab8898; rabbit anti-H3K27me3, Cell Signaling Technology C36B11; rabbit anti-H3K4me3 Active Motif 39159 ...
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bioRxiv - Cancer Biology 2020Quote: ... Supershift analysis was carried out by antibodies to RUNX 1 or RUNX 3 (Active Motif cat# 39300 and 39301, respectively). The gel was exposed to X ray film and/or phosphorimaging screens ...
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bioRxiv - Neuroscience 2022Quote: ChIP-seq libraries were constructed from FACS-enriched RGCs (100,000 cells for each library) using an H3K27me3 antibody (1:100, Active Motif) or normal rabbit IgG (1:100 ...
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bioRxiv - Cancer Biology 2024Quote: ... incubated overnight at 4 °C with 1: 1000 primary antibody diluted in blocking solution (H3K9me3, Active Motif; H3, Santa Cruz) followed by secondary antibody diluted 1 ...
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bioRxiv - Cancer Biology 2024Quote: ... The membranes were then blocked in 5% milk/TBST for 1 h followed by primary antibody incubation at 4 °C overnight at dilution of 1:10,000 rabbit anti hmC (catalog no. 39069, Active Motif). The blots were washed with TBST followed by incubation with secondary 1:3,000 anti-rabbit-HRP for 5-hmC for 1 h ...
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bioRxiv - Cancer Biology 2024Quote: ... and nuclei were isolated and incubated overnight with Concanavalin A beads and 1.3 µl of the primary anti-H3K27me3 or anti-H3K27Ac antibody (Active Motif 39156 ...
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bioRxiv - Cancer Biology 2024Quote: ... Genomic DNA regions of interest were isolated using 5 µg of antibody against MITF (Active Motif, cat# 39789, lot# 11313002). Complexes were washed ...
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bioRxiv - Cancer Biology 2024Quote: ... 5% of soluble chromatin was kept for input DNA and rest of chromatin was used to perform immunoprecipitation with 7.5μg of H4K20me1 antibody (Active Motif #39727) overnight at 4°C with rotation ...
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bioRxiv - Molecular Biology 2021Quote: ... precleared chromatin was incubated overnight at 4 °C with 5 μg of the following antibodies: H3K4me2 (39141; Active Motif, Carlsbad, USA), H3K9me3 (39161 ...
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bioRxiv - Biophysics 2021Quote: ... Supplementary Figure 12 and Supplementary Movie 8 show data of a 2% formaldehyde fixed C127 cell immune-labelled with rabbit anti-histone H3K4me3 primary antibodies (Active Motif) and goat-anti rabbit Alexa Fluor 488 secondary antibodies (ThermoFisher) ...
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bioRxiv - Immunology 2022Quote: ... 1 × 105 BM pDCs sorted from Hdac3fl/fl control or Hdac3fl/fl CreERT2 littermates were bound to beads and were subjected for immunoprecipitation with primary anti-H3K27ac antibody (Active Motif) at 4°C overnight ...
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bioRxiv - Genomics 2020Quote: ... The following primary antibodies were purchased and used for immunofluorescence and CUT&RUN27,28 followed by sequencing: Rabbit anti-H3K4me3 (Active motif, 39159), Rabbit anti-H3K27me3 (Active Motif ...
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bioRxiv - Developmental Biology 2020Quote: ... ChIP-Seq of T7 and MED12 were done in the presence of Drosophila spike-in chromatin and antibody following the manufacturers’ protocol (Active Motif). ChIP-Seq library construction was done using NEBNext Ultra™ II DNA Library Prep Kit for Illumina (NEB ...
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bioRxiv - Cancer Biology 2021Quote: ... and a p300 antibody (Clone NM11, 1:200 for ChIP, 1:1000 for IB, 1:100 for IP) were purchased from Active Motif. The antibody against cMyc-AcK148 was prepared in a previous study 28 ...
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bioRxiv - Cancer Biology 2020Quote: ... The membranes were blocked in 5 % milk/TBST for 1 h followed by primary antibody blotting at 4 °C overnight incubation – 1: 2000 rabbit anti-hmC (cat# 39069, Active Motif). The next day the blots were washed with TBST followed by incubation with secondary 1:2000 anti-rabbit-HRP for 1 h ...
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bioRxiv - Immunology 2020Quote: ... and then incubated for 2 hours at room temperature with respective primary antibodies (at dilutions of 1:100): H3K27me3 (39155, Active Motif); Ubiquityl-histone H2A Lys119 (8240 ...
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bioRxiv - Genomics 2022Quote: ... 0.25% NP-40) and incubated for 1 h with 10 μg/IP of mouse bridging antibody (Active Motif; 1 μg/μl) at 4°C with end-to-end rotation ...
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bioRxiv - Neuroscience 2022Quote: ... ChIP reactions were carried out overnight at 4 °C with 3 µg of sheared chromatin and 10 μg of anti-H3K27me3 rabbit polyclonal antibody (39155, Active Motif) or anti-mouse IgG as negative control (115-001-003 ...
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bioRxiv - Molecular Biology 2023Quote: ... the cells were incubated with anti-5mC antibody (1:200 diluted in 0.5% goat serum + PBST, 39649, Active Motif, CA, USA) at 4 °C overnight and then washed three times with PBST for 5 min each ...
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bioRxiv - Genetics 2023Quote: ... membranes were incubated overnight with the following antibodies diluted at 1:2,500: anti-H3 (clone 1B1B2) (Cell Signalling Technology, #14269]) anti-H3K9ac (Active Motif, #39137) anti-H3K27ac [Active Motif ...
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bioRxiv - Genomics 2024Quote: ... The membrane was then blocked in 5% skimmed milk in TBS containing 0.1% Tween 20 (TBST) for 1 h at room temperature followed by incubation with 1:10,000 dilution of 5hmC antibody (Active Motif, 39069) overnight at 4°C ...
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bioRxiv - Immunology 2024Quote: ... Cells bound to the beads were resuspended in Dig-Wash Buffer and incubated with a 1:50 dilution of primary antibodies (rabbit anti-H3K4me3, Active Motif,39016 ...
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bioRxiv - Cell Biology 2024Quote: ... and incubated with a 1:500 dilution of the appropriate primary antibody for 1 h at room temperature (anti-CTCF, Active Motif 61331 ...
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bioRxiv - Cancer Biology 2024Quote: ... were denatured by heating to 98C for 10min and chilled on ice for 10min and incubated overnight with anti-5hmC antibody (4μl, Active Motif #39791) in 20mM Na-Phosphate pH 7.0 ...
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bioRxiv - Cancer Biology 2021Quote: ... Relative 5hmC levels were quantified blotting 500 ng of gDNA on a nitrocellulose membrane with an anti-5hmC antibody (Active Motif, 39769) diluted 1/10000 followed by an anti-rabbit antibody coupled to horseradish peroxydase (Dutscher NA934 ...
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bioRxiv - Genomics 2020Quote: ... and incubated with the appropriate primary antibody for 1 h at room temperature (anti-RNAPII: 1:500, 61086, Active Motif; anti-H3K27ac: 1:500, 39133, Active Motif; anti-H3K27me3 ...
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bioRxiv - Microbiology 2022Quote: ... Membranes were blocked in 5% milk in PBS-T for 1 h at room temperature and incubated overnight at 4°C with primary antibodies against Lamin AC (Active Motif 39287), Lamin B1 (Abcam ab16048) ...
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Targeted attenuation of elevated histone marks at SNCA alleviates α-synuclein in Parkinson’s diseasebioRxiv - Neuroscience 2020Quote: ... the following primary antibodies were used: (Normal mouse IgG, Millipore, 12-371; H3K4me3, Abcam, ab8580; H3K27me3, Active motif, 39155; H3K27ac, Abcam, ab4729). Anti-Cas9 antibody (Takara ...
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bioRxiv - Developmental Biology 2020Quote: ... Beads were then resuspended in Blocking buffer and coated with antibodies for 4 h at 4°C (H4K20me1 [0.5 μg/IP], Active Motif cat. 39727). Once coated beads were magnet-separated and resuspended in 1 mL of lysate ...
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bioRxiv - Developmental Biology 2023Quote: ... and chromatin was incubated for 2h at 4°C with 5 μg of anti-H3K27ac antibody (Active Motif, Cat# 39133, Lot 01613007). Immuno-complexes were sequentially washed ...
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bioRxiv - Molecular Biology 2022Quote: ... Cell fractionations were verified by western blot as described in 57 with antibodies against RNA polymerase II CTD phospho Ser2 (Active Motif, 61984) and GAPDH (GeneTex GT239 or ThermoFisher MA5-15738) ...
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bioRxiv - Cell Biology 2024Quote: ... The H3K27Ac ChIP reactions were conducted with 10 μg of tissue chromatin and 4 μg of H3K27Ac antibody (Active Motif, cat # 39133). The H3K4me1 ChIP reactions were carried out by using 10 ug of tissue chromatin and 4 ul of H3K4me1 antibody (Active Motif ...
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bioRxiv - Molecular Biology 2024Quote: ... Primary antibodies (anti-BRD4, Cell Signaling Technologies Mouse mAb #63759 at 1:1000 dilution, anti-H3K27ac, Active Motif #39133, 1:1000 dilution) were prepared in blocking buffer and incubated on the sample overnight at 4 °C ...
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bioRxiv - Microbiology 2023Quote: ... Beads were resuspended in 100 μl of IP/wash buffer and coated with 5 μg of rabbit anti-m6A antibody (Active motif, 61495) for 45 min at room temperature with rotation ...
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bioRxiv - Developmental Biology 2023Quote: ... ChIP assay was done using SimpleChIP Plus Enzymatic Chromatin IP kit (magnetic beads) #9005 from CellSignalling and H3K27me3 antibody (Active Motif RRID:AB_2561020). ChIP samples were prepared with Kapa Hyper prep kit ...