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Citations for Active Motif :
401 - 429 of 429 citations for Ectonucleotide pyrophosphatase phosphodiesterase family member 7 ENPP7 Antibody HRP since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2024Quote: ... Primary antibodies (anti-BRD4, Cell Signaling Technologies Mouse mAb #63759 at 1:1000 dilution, anti-H3K27ac, Active Motif #39133, 1:1000 dilution) were prepared in blocking buffer and incubated on the sample overnight at 4 °C ...
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bioRxiv - Microbiology 2023Quote: ... Beads were resuspended in 100 μl of IP/wash buffer and coated with 5 μg of rabbit anti-m6A antibody (Active motif, 61495) for 45 min at room temperature with rotation ...
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bioRxiv - Developmental Biology 2023Quote: ... ChIP assay was done using SimpleChIP Plus Enzymatic Chromatin IP kit (magnetic beads) #9005 from CellSignalling and H3K27me3 antibody (Active Motif RRID:AB_2561020). ChIP samples were prepared with Kapa Hyper prep kit ...
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bioRxiv - Cell Biology 2024Quote: ... For assessing histone modification 70 μg of chromatin from WT and KO cells was taken and 3ug antibodies were added H3K27ac (Active motif, #39134), H3K27me3 (Abcam ...
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bioRxiv - Genomics 2024Quote: ... Cell fractionations were verified by western blot as described in (Mayer and Churchman 2016) with antibodies against RNA polymerase II CTD phospho Ser2 (Active Motif, 61984) and GAPDH (ThermoFisher MA5-15738).
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bioRxiv - Cell Biology 2024Quote: ... Pre-cleared chromatin samples were incubated overnight at 4°C on a rotator with histone modification antibodies–either anti-H3K4me3 (Active Motif, 39159), anti-H3K27ac (Abcam ...
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bioRxiv - Genomics 2024Quote: ... were bound with antibodies of interests (H3K27ac, Active Motif 39133; H3K4me3, Active Motif 39915; H3K9me3, Abcam ab176916 or H3K27me3, Active Motif 39155) and incubated at 4°C for 36 hours ...
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bioRxiv - Developmental Biology 2024Quote: ... using 18 μg of sheared chromatin from 30 pooled embryonic testes and 5 μl of COUP-TFII antibody (Active Motif, CA 61213). Libraries were sequenced by Active Motif as single-end 75-mers on an Illumina NextSeq 500 instrument.
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bioRxiv - Cell Biology 2020Quote: ... Primary antibodies (DSHB: Pax7: PAX7, eMyHC: F1.652, MHC:20-s, Abcam: Tfr1: ab84036 and Active Motif: MyoD:39991; Ki67:ZM-0167, ZSGB-Bio) were incubated overnight at 4 °C and secondary antibodies at RT for 1 hr at dark ...
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bioRxiv - Molecular Biology 2022Quote: ... The following antibodies and dilutions were used: anti-Esrrb (PP-H6705-00, Perseus Proteomics, 1:1000) and anti-Histone H3 (61476, Active Motif, 1:5000).
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bioRxiv - Cell Biology 2024Quote: ... The H3K4me1 ChIP reactions were carried out by using 10 ug of tissue chromatin and 4 ul of H3K4me1 antibody (Active Motif, cat # 39297). Libraries were prepared by a custom Illumina library with the standard Illumina PE adaptors (Bentley ...
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bioRxiv - Microbiology 2024Quote: ... The primary antibodies were used at the following dilutions: anti-Cas9 (1:1000 in 5% milk/PBS-T, Active Motif, clone 7A9-3A3); anti-EF1α (1:20,000 in 1% milk/PBS-T ...
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bioRxiv - Molecular Biology 2023Quote: ... and immunoprecipitation performed over night at 4 °C on a spinning wheel by adding 10 µl of the respective antibody (Anti H3K27Me3 #9733 Cell Signaling, Anti H3 #2650 Cell Signaling, Anti H3K56Ac #61061 Active Motif). Afterwards ...
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bioRxiv - Genetics 2022Quote: ... Around 20 μg or 10 μg of chromatin were incubated overnight at 4°C with 10 μg H3.X/Y or 2.5 μg H3K9me3 antibody respectively (Active Motif-61161, Abcam-ab8898). Chromatinantibody extracted with incubated with protein G Dynal beads (Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2023Quote: ... All incubations with primary antibodies were performed for either 1h at room temperature or overnight at 4°C with the following antibodies for H3K9me3 (Active Motif, 1:1000), DAXX (Santa Cruz ...
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bioRxiv - Genetics 2024Quote: ... followed by a 1 hour incubation with the appropriate primary antibody (H3K27me1; Active Motif #61015, H3K27me3, Millipore #07-449, H3K27M, Active Motif #61803) diluted in Abdil-Tx ...
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bioRxiv - Molecular Biology 2021Quote: ... Immunoprecipitations for H2A.Z and HA-tag were performed similarly on benzonase-treated yeast cell extract using 36µl Protein-A sepharose beads coupled with 6µl of appropriate antibody (Anti-H2A.Z, Active Motif 39647; Anti-HA tag, Abcam ab9110). Control immunoprecipitations (mock ...
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bioRxiv - Genomics 2021Quote: ... The DNA was denatured for 10 min at 95°C and immunoprecipitated overnight at 4°C with 4 μL of 5hmC monoclonal antibody (Active Motif Cat No 55010). To allow selective enrichment of immune-captured DNA fragments ...
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bioRxiv - Cell Biology 2021Quote: ... while negative control was incubated with mouse IgG and input DNA without antibody using the CHIP—IT Express HT Kit from Active Motif (Cat no: 53018) according to the manufacturer’s instructions [Supplementary Table III] ...
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bioRxiv - Biochemistry 2021Quote: ... rabbit polyclonal anti-Pre6 (gift from D. Wolf, 1:5000) and anti-Rpb1 (RNA Pol II) monoclonal mouse antibody (Active Motif, 4H8, 1:2000). For rabbit primary antibodies ...
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bioRxiv - Cell Biology 2021Quote: ... while negative control was incubated with mouse IgG and input DNA without antibody using the CHIP—IT Express HT Kit from Active Motif (Cat no: 53018) according to the manufacturer’s instructions [Supplementary Table III] ...
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bioRxiv - Immunology 2024Quote: ... cells were incubated overnight with Concanavalin A beads and 1 µl of the primary anti-H3K4me1 antibody per reaction (Active Motif, catalog number 39297) and 1 µl of the primary anti-H3K27ac antibody per reaction (Active Motif ...
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bioRxiv - Cancer Biology 2024Quote: ... and nuclei were isolated and incubated overnight with Concanavalin A beads and 1.3 µl of the primary anti-H3K27me3 or anti-H3K27Ac antibody (Active Motif 39156, 23254116-11; Active Motif 39135, 23061102-11) per reaction ...
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bioRxiv - Developmental Biology 2022Quote: ... Antibody incubation (DUX: sc-137190; Santa Cruz Biotechnology, Santa Cruz, CA, USA; SIRT1: 8469S; CST, Danvers, MA, USA; H3K27ac: 39034; Active Motif, Carlsbad, CA, USA) was performed overnight at 4°C ...
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bioRxiv - Genomics 2020Quote: ... were raised against KLH-conjugated dinucleosides at the Technology Development Laboratory (Babraham Bioscience Technologies Ltd, Cambridge, UK) (anti-mCA antibody clone 2C8H8A6 currently available from Active Motif, Cat. No 61783/4). Alexa Fluor secondary antibodies were purchased from Life Technologies ...
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bioRxiv - Genomics 2020Quote: ... the RevMAb Biosciences (clone RM362, at 1:300 dilution) and the mouse monoclonal recombinant AbFlex m6A antibody (rAb) from Active Motif (at 1:300 dilution). A mixture of reference DNA oligonucleotides that correspond to the position of the modified nucleotides was used to compare with antibody binding (the last base at the 5’ end of the oligonucleotide hybridizes with the modified base ...
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bioRxiv - Developmental Biology 2020Quote: ... pre-cleared for 2 h followed by immunoprecipitation with primary antibodies for 12-16 hours at 4°C (H3K27ac, Active motif 39133; H3K27me3, CST 9733s). 5% of samples were used as input DNA ...
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bioRxiv - Molecular Biology 2024Quote: ... H3K27ac histone modification data was generated from liver sinusoidal endothelial (LSEC) and liver-resident immune cryopreserved cells using the Cut&Tag-IT Assay kit (Active Motif, H3K27ac antibody cat#39133). Library products were assessed by Agilent Bioanalyzer HS DNA assays (Agilent Technologies ...
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bioRxiv - Molecular Biology 2022Quote: ... After an overnight incubation at 4°C with the primary antibodies (anti-5mC, Eurogentec, ref BI-MECY-0100, 1/500; anti-5hmC, Active Motif, ref 39569, 1/500), and a 1-hour incubation at room temperature with secondary antibodies (Dylight 488 anti-mouse ...