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Citations for Active Motif :
401 - 450 of 505 citations for B Cell Activating Factor BAFF TNFSF13B Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2022Quote: ... ChIP reactions were carried out overnight at 4 °C with 3 µg of sheared chromatin and 10 μg of anti-H3K27me3 rabbit polyclonal antibody (39155, Active Motif) or anti-mouse IgG as negative control (115-001-003 ...
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bioRxiv - Genomics 2023Quote: ... The membrane was then blocked in 5% skimmed milk in TBS containing 0.1% Tween 20 (TBST) for 1 h at room temperature followed by incubation with 1:10,000 dilution of 5hmC antibody (Active Motif, 39069) overnight at 4°C ...
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bioRxiv - Molecular Biology 2023Quote: ... Antibodies against target histone modifications were used to detect respective bands (Anti H3K27Me3 #9733 Cell Signaling, Anti H3K9Me #ABE101 Merck Millipore, Anti H3K56Ac #61061 Active Motif, Anti H3K9Ac #39038 Active Motif, Anti H3 #9715 Cell Signaling), detection protocols have been summarized in Supplemental Table 4 ...
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bioRxiv - Cancer Biology 2021Quote: ... Relative 5hmC levels were quantified blotting 500 ng of gDNA on a nitrocellulose membrane with an anti-5hmC antibody (Active Motif, 39769) diluted 1/10000 followed by an anti-rabbit antibody coupled to horseradish peroxydase (Dutscher NA934 ...
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bioRxiv - Genomics 2020Quote: ... and incubated with the appropriate primary antibody for 1 h at room temperature (anti-RNAPII: 1:500, 61086, Active Motif; anti-H3K27ac: 1:500, 39133, Active Motif; anti-H3K27me3 ...
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bioRxiv - Microbiology 2022Quote: ... Membranes were blocked in 5% milk in PBS-T for 1 h at room temperature and incubated overnight at 4°C with primary antibodies against Lamin AC (Active Motif 39287), Lamin B1 (Abcam ab16048) ...
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bioRxiv - Genomics 2019Quote: ... We performed western blots against both GAPDH (GAPDH antibody (6C5) Life Technologies AM4300) and the Carboxyl Terminal Domain of Pol-II (CTD) (Pol II CTD Ser5-P antibody, Active Motif, 61085). We ran each lysate on Mini-protean TGX precast gels (bioRad 456-1093 ...
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bioRxiv - Molecular Biology 2019Quote: ... and coupled to one of the following antibodies in the same buffer for 1 hr at room temperature with rotation: 3 μl H3 (Active motif 39763), 5 μl H3K4me3 (Millipore 04-745) ...
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Targeted attenuation of elevated histone marks at SNCA alleviates α-synuclein in Parkinson’s diseasebioRxiv - Neuroscience 2020Quote: ... the following primary antibodies were used: (Normal mouse IgG, Millipore, 12-371; H3K4me3, Abcam, ab8580; H3K27me3, Active motif, 39155; H3K27ac, Abcam, ab4729). Anti-Cas9 antibody (Takara ...
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bioRxiv - Developmental Biology 2020Quote: ... Beads were then resuspended in Blocking buffer and coated with antibodies for 4 h at 4°C (H4K20me1 [0.5 μg/IP], Active Motif cat. 39727). Once coated beads were magnet-separated and resuspended in 1 mL of lysate ...
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bioRxiv - Molecular Biology 2022Quote: ... Cell fractionations were verified by western blot as described in 57 with antibodies against RNA polymerase II CTD phospho Ser2 (Active Motif, 61984) and GAPDH (GeneTex GT239 or ThermoFisher MA5-15738) ...
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bioRxiv - Developmental Biology 2023Quote: ... and chromatin was incubated for 2h at 4°C with 5 μg of anti-H3K27ac antibody (Active Motif, Cat# 39133, Lot 01613007). Immuno-complexes were sequentially washed ...
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bioRxiv - Developmental Biology 2023Quote: ... ChIP assay was done using SimpleChIP Plus Enzymatic Chromatin IP kit (magnetic beads) #9005 from CellSignalling and H3K27me3 antibody (Active Motif RRID:AB_2561020). ChIP samples were prepared with Kapa Hyper prep kit ...
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bioRxiv - Microbiology 2023Quote: ... Beads were resuspended in 100 μl of IP/wash buffer and coated with 5 μg of rabbit anti-m6A antibody (Active motif, 61495) for 45 min at room temperature with rotation ...
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bioRxiv - Cell Biology 2024Quote: ... The H3K27Ac ChIP reactions were conducted with 10 μg of tissue chromatin and 4 μg of H3K27Ac antibody (Active Motif, cat # 39133). The H3K4me1 ChIP reactions were carried out by using 10 ug of tissue chromatin and 4 ul of H3K4me1 antibody (Active Motif ...
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bioRxiv - Cell Biology 2020Quote: ... cells were processed via the ChIP-IT High Sensitivity kit (Active motif) as per manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2021Quote: ... cells were processed via the ChIP-IT High Sensitivity kit (Active motif) as per manufacturer’s instructions ...
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bioRxiv - Genomics 2020Quote: ... cells were processed via the ChIP-IT High Sensitivity kit (Active motif) as per manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: ... and transformed into Rapid Trans™ chemically competent TAM1 cells (Active Motif).
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bioRxiv - Immunology 2022Quote: ... The cells were then incubated with primary anti-5hmC (10013602; Active Motif) overnight at 4°C and the day after with secondary antibody for 1 hr at room temperature ...
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bioRxiv - Immunology 2022Quote: ... The cells were then incubated with primary anti-5hmC (10013602; Active Motif) overnight at 4°C and the day after with secondary antibody for 1 hr at room temperature ...
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bioRxiv - Genomics 2023Quote: ... cells were processed via the ChIP-IT High Sensitivity kit (Active motif) as per the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2023Quote: ... rat anti-dCENP-A for Kc167 cells (AB_2793749, 1:100, Active motif), rabbit anti-dCENP-A for ovaries (AB_2793320 ...
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bioRxiv - Cell Biology 2020Quote: ... Primary antibodies (DSHB: Pax7: PAX7, eMyHC: F1.652, MHC:20-s, Abcam: Tfr1: ab84036 and Active Motif: MyoD:39991; Ki67:ZM-0167, ZSGB-Bio) were incubated overnight at 4 °C and secondary antibodies at RT for 1 hr at dark ...
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bioRxiv - Molecular Biology 2022Quote: ... The following antibodies and dilutions were used: anti-Esrrb (PP-H6705-00, Perseus Proteomics, 1:1000) and anti-Histone H3 (61476, Active Motif, 1:5000).
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bioRxiv - Genetics 2022Quote: ... Around 20 μg or 10 μg of chromatin were incubated overnight at 4°C with 10 μg H3.X/Y or 2.5 μg H3K9me3 antibody respectively (Active Motif-61161, Abcam-ab8898). Chromatinantibody extracted with incubated with protein G Dynal beads (Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2023Quote: ... All incubations with primary antibodies were performed for either 1h at room temperature or overnight at 4°C with the following antibodies for H3K9me3 (Active Motif, 1:1000), DAXX (Santa Cruz ...
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bioRxiv - Developmental Biology 2023Quote: ... Human induced pluripotent stem cell derived cardiomyocytes (iPSC-CMs) from four HLHS patients were fixed in 4% paraformaldehyde and stained with antibody against 5-methylcytosine (Active Motif, RRID: AB_2687950)31 ...
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bioRxiv - Microbiology 2024Quote: ... The primary antibodies were used at the following dilutions: anti-Cas9 (1:1000 in 5% milk/PBS-T, Active Motif, clone 7A9-3A3); anti-EF1α (1:20,000 in 1% milk/PBS-T ...
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bioRxiv - Cell Biology 2024Quote: ... The H3K4me1 ChIP reactions were carried out by using 10 ug of tissue chromatin and 4 ul of H3K4me1 antibody (Active Motif, cat # 39297). Libraries were prepared by a custom Illumina library with the standard Illumina PE adaptors (Bentley ...
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bioRxiv - Developmental Biology 2021Quote: ... followed by immunoprecipitation according to the Low Cell ChIPseq kit (Active Motif, 53084) with some modifications ...
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bioRxiv - Molecular Biology 2022Quote: ... 5 µl (50 ng) of Drosophila chromatin from S2 cells (Active Motif, #53083) was added as spike-in control ...
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bioRxiv - Microbiology 2021Quote: ... RASGTP levels in cells were quantified using RASGTP ELISA kit (Active Motif, 52097) per manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2020Quote: ... Cell lysates were immunoprecipitated using Universal Magnetic Co-IP Kit (Active Motif 54002), then analyzed by western blot ...
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bioRxiv - Molecular Biology 2023Quote: ... 0.2 mM dithiothreitol (DTT)) and sonicated for cell lysis (Q120 Sonicator, Active Motif). After centrifugation for 1 h at 4 °C and 45,850 g ...
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bioRxiv - Genomics 2023Quote: ... 0.5 million cells were processed using the CUT&Tag-IT kit (Active Motif) as per manufacturer’s instructions and the resulting libraries were paired-end sequenced on a NextSeq500 platform (Illumina ...
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bioRxiv - Genomics 2023Quote: ... Cells were then processed with the ChIP-IT High Sensitivity kit (Active motif) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2023Quote: ... cells were washed and blocked using the MAXpack Immunostaining Media Kit (Active Motif). Cells were incubated overnight at 4 °C in primary antibody (1:100 Abcam Rabbit anti-Atrx ab97508) ...
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bioRxiv - Genomics 2021Quote: ... The DNA was denatured for 10 min at 95°C and immunoprecipitated overnight at 4°C with 4 μL of 5hmC monoclonal antibody (Active Motif Cat No 55010). To allow selective enrichment of immune-captured DNA fragments ...
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bioRxiv - Cell Biology 2021Quote: ... while negative control was incubated with mouse IgG and input DNA without antibody using the CHIP—IT Express HT Kit from Active Motif (Cat no: 53018) according to the manufacturer’s instructions [Supplementary Table III] ...
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bioRxiv - Biochemistry 2021Quote: ... rabbit polyclonal anti-Pre6 (gift from D. Wolf, 1:5000) and anti-Rpb1 (RNA Pol II) monoclonal mouse antibody (Active Motif, 4H8, 1:2000). For rabbit primary antibodies ...
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bioRxiv - Cell Biology 2021Quote: ... while negative control was incubated with mouse IgG and input DNA without antibody using the CHIP—IT Express HT Kit from Active Motif (Cat no: 53018) according to the manufacturer’s instructions [Supplementary Table III] ...
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bioRxiv - Cell Biology 2020Quote: ... Fibroblast cell nuclear fraction was extracted using a commercially available kit (Active motif, 40010) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2019Quote: Cytoplasmic extracts were generated by disrupting cells using hypotonic buffer (Active Motif, Carlsbad, CA) and pelleting the cell debris by centrifugation at 14000 × g for 10 min at 4 °C ...
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bioRxiv - Biophysics 2022Quote: ... and P1 virus was generated from cells transfected with FuGENE transfection reagent (Active Motif) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2020Quote: ... Fibroblast cell nuclear fraction was extracted using a commercially available kit (Active motif, 40010) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2021Quote: Chromatin immunoprecipitation was performed using the Low Cell ChIP-Seq kit (#53048, Active Motif) following the manufacturer’s instructions ...
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bioRxiv - Pathology 2023Quote: ... Cells were lysed to ChIP-IT Express Magnetic Chromatin Immunoprecipitation kit (Active Motif 53008). Optimal sonication conditions were determined previously in order to obtain DNA fragments of about 500 bp ...
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bioRxiv - Microbiology 2023Quote: ... Cells were lysed using 100 µl of ice-cold ATAC lysis buffer (Active Motif), followed immediately by addition of 1 ml pre-chilled wash buffer (10 mM Tris-HCl pH 7.4 ...
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bioRxiv - Developmental Biology 2022Quote: ... Antibody incubation (DUX: sc-137190; Santa Cruz Biotechnology, Santa Cruz, CA, USA; SIRT1: 8469S; CST, Danvers, MA, USA; H3K27ac: 39034; Active Motif, Carlsbad, CA, USA) was performed overnight at 4°C ...