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Citations for Active Motif :
301 - 350 of 388 citations for BCA Protein Colorimetric Detection Kit 2 Plate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2022Quote: CUT&Tag was performed from 450k nuclei using the Cut&TagIT Kit following manufacturer’s protocol (Active Motif).
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bioRxiv - Immunology 2019Quote: ... Subsequent chromatin preparation steps were performed according to the “High Sensitivity Chromatin Preparation” Kit (Active Motif, 53046). Samples were sonicated two times for 10 minutes (30 seconds on ...
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bioRxiv - Developmental Biology 2021Quote: ChIP experiments were carried out using the ChIP-IT Express Enzymatic kit (Cat. No. 53009, Active Motif) or the True MicroChIP Kit (Cat ...
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bioRxiv - Developmental Biology 2021Quote: ... The supernatant was removed and the beads washed with 665µL of ChIP Buffer (from Active Motif kit) by inverting the tube several times ...
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bioRxiv - Molecular Biology 2021Quote: ATAC-seq was performed in biological duplicate using the ATAC-Seq Kit (Active Motif, catalog No.53150). Briefly ...
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bioRxiv - Cell Biology 2023Quote: ... ChIP was then performed using a ChIP-IT High Sensitivity kit (#53040, Active Motif, Carlsbad, CA, USA) according to the manufacturer’s instructions ...
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bioRxiv - Genomics 2024Quote: ... and Illumina sequencing libraires were prepared using the Low Cell ChIP-Seq kit (Active Motif, Carlsbad, CA) according to the manufacturer instructions ...
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bioRxiv - Neuroscience 2022Quote: For DNA Chromatin Immunoprecipitation (ChIP) we used the ChIP-IT High Sensitivity® kit from Active motif. Lysates were obtained as described in the RIP protocol ...
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bioRxiv - Cell Biology 2023Quote: ... and nuclei was extracted using ice-cold ATAC-lysis buffer (according to the kit protocol; Active Motif) and the pellet resuspended in the Tn5 tagmentation master mix ...
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bioRxiv - Molecular Biology 2023Quote: ... ChIP-seq libraries were constructed using the Next Gen DNA Library Kit (Active Motif, 53216 and 53264). Library quality was assessed using Agilent 2100 Bioanalyzer and Agilent High Sensitivity DNA assay ...
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bioRxiv - Neuroscience 2023Quote: ... and secondary antibody was guinea pig anti-rabbit (Active Motif, 105465 from CUT&TagIT kit, 1:100).
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bioRxiv - Microbiology 2023Quote: ... Histones were isolated from inhibitor treated or untreated cells using the histone extraction kit (Active Motif, 40028) and western blots performed ...
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bioRxiv - Cancer Biology 2024Quote: ... The protocol was followed to produce Illumina compatible libraries using the ATAC-seq kit from Active Motif. Briefly ...
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bioRxiv - Molecular Biology 2024Quote: CUT&Tag experiments were performed in duplicates using the CUT&Tag-IT Assay Kit (Active Motif #53160) and following manufacturer instructions ...
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bioRxiv - Microbiology 2020Quote: ... Chromatin immunoprecipitation was performed using the ChIP-IT Express Enzymatic kit according to the manufacturer’s directions (Active Motif). DNA was enzymatically sheared for 10 min ...
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bioRxiv - Cancer Biology 2021Quote: ... fractionation of MDA-MB-231 and 4T1 cells was first performed using the Nuclear Extract kit (Active Motif). The level of protein binding to DNA consensus sequence was then assessed by ELISA using the TransAM NF-kB Family kit (Active Motif ...
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bioRxiv - Cell Biology 2020Quote: Purified DNA fragments for ChIP-seq were prepared by using the ChIP-IT High Sensitivity Kit (Active Motif) and the related antibodies ...
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bioRxiv - Genomics 2019Quote: ... The libraries were generated with the Illumina Nextera DNA Library Prep kit and were sequenced by Active Motif and NIEHS Sequencing core by Illumina HiSeq 2000 and NextSeq 500 systems with 42bp paired-end reads on each sample ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... the nucleoproteins were extracted and detected using the Trans-AM NF-κB kits (Active Motif, Carlsbad, CA, USA).
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bioRxiv - Molecular Biology 2022Quote: ... ATAC-seq was performed as described previously (Buenrostro et al., 2015) using an ATAC-seq kit (Active Motif) as described by the manufacturer ...
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bioRxiv - Genomics 2022Quote: ... histones were extracted according to the recommended manufacturer’s one-step protocol using the Histone Extraction Kit (Active Motif). The protein concentration of the lysates was determined using the BCA Protein Assay Kit (Thermo Fisher Scientific) ...
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bioRxiv - Developmental Biology 2020Quote: ... samples were Polytron minced and homogenized by douncing in Prep Buffer (ChIP-IT High Sensitivity Kit, Active Motif). They were then sonicated in 100 µl of sonication buffer (0.1%SDS ...
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bioRxiv - Cell Biology 2021Quote: Cytoplasm and nucleus of keratinocytes were separated by using Nuclear Extract Kit (Active Motif, Inc., Carlsbad, CA, USA) following the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2024Quote: Nrf2 activity was quantified using nuclear extracts and a TransAm NRF2 activity kit from Active Motif (cat# 50296) following the manufacturers included instructions.
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bioRxiv - Cancer Biology 2024Quote: ... Cell pellets used for fractionation were fractionated using the Nuclear Complex Co-IP kit from Active motif (#54001) according to their protocol.
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bioRxiv - Genomics 2024Quote: Human islet ATAC-seq libraries were prepared following the Active motif ATAC prep kit (Active motif catalog# 53150). Briefly ...
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Small Molecule Screen Identifies Pyrimethamine as an Inhibitor of NRF2-driven Esophageal HyperplasiabioRxiv - Cancer Biology 2022Quote: NRF2-ARE binding assay was performed with an ELISA-based TransAM NRF2 Assay Kit (Active Motif, Carlsbad, CA) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2023Quote: Chromatin immunoprecipitation (ChIP) assays were conducted using the ChIP-IT EXPRESS assay kit protocol (Active Motif, Carlsbad, CA). After exposure to Aβo ...
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bioRxiv - Immunology 2023Quote: ... Chromatin immunoprecipitation (ChIP) was carried out using the ChIP-IT Express Enzymatic kit from Active Motif (Rixensart, Belgium). In brief ...
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bioRxiv - Molecular Biology 2023Quote: ... Chromatin for re-ChIP was prepared using the ChIP-IT High Sensitivity Kit (Active Motif, catalog no. 53040), as described above ...
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bioRxiv - Molecular Biology 2020Quote: ... Global % 5mC levels were assayed using a Global DNA Methylation LINE-1 Kit (55017; Active Motif, Carlsbad, CA, USA) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2021Quote: Cells from two independent experiments were collected and open chromatin was assessed using an ATACseq kit (Active Motif, 53150). DNAseq library preparation was completed at the Oncogenomics Core Facility at the Sylvester Comprehensive Cancer Center ...
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bioRxiv - Cell Biology 2020Quote: ... IL-13 effects on NFκB p52 DNA binding was evaluated using the TransAM NFκB Transcription Factor Assay Kit (Active Motif). The samples were run in duplicates and absorbance was measured using a spectrophotometer at 450 nm.
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bioRxiv - Molecular Biology 2021Quote: Chromatin was prepared from flash-frozen liver tissue using the Active Motif ChIP-IT High Sensitivity kit (Active Motif), employing a modified protocol described in (47) ...
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bioRxiv - Cancer Biology 2023Quote: ... Nuclear fractions were extracted following the manufacturer’s instruction supplied with the Universal Magnetic co-IP kit (54002, Active Motif). 1000μg of proteins were immunoprecipitated using 5.0μg of antibody followed by protein G magnetic beads ...
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bioRxiv - Cell Biology 2020Quote: ... and nuclear extracts were isolated using the reagents and protocol provided in the Active Motif Nuclear Extract Kit (Active Motif). IL-13 effects on NFκB p52 DNA binding was evaluated using the TransAM NFκB Transcription Factor Assay Kit (Active Motif) ...
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bioRxiv - Developmental Biology 2019Quote: ... All H3K27ac ChIP experiments were processed as ChIP-seqs using the reagents from ChIP-IT High Sensitive kit (Active Motif). IPs were performed in parallel technical duplicates with 11 to 14µg of chromatin on each ...
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bioRxiv - Microbiology 2019Quote: ... Cell fixation was performed using the fixation reagents and steps from the Low Cell Chip Kit (Active Motif; Cat#53084) as described in the user manual ...
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bioRxiv - Microbiology 2019Quote: Immunoprecipitation of both RNA-DNA hybrids (DRIP) and γH2A was performed using the ChIP-IT Enzymatic Express kit (Active Motif) with formaldehyde fixed chromatin samples ...
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bioRxiv - Immunology 2020Quote: ... Nuclear extract from HuT78 cells (endogenous SP140) was prepared following manufacturer’s protocol (Active Motif nuclear extract and co-IP kit). Protein concentration was determined by Bradford Assay (Pierce) ...
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bioRxiv - Immunology 2024Quote: ... and total RNA fractions were prepared from A549 cells according to the RNA Subcellular Isolation Kit (Active Motif, Cat. #25501). cDNA was prepared as described above and qPCR was performed to analyze VILMIR expression in both cellular fractions ...
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bioRxiv - Cancer Biology 2023Quote: ChIP assays at the HES1 promoter were performed in HT29 cells using the CHIP-IT kit (Active Motif, Carlsbad, USA). Sonicated chromatin was immunoprecipitated with antibodies against IgG (sc-3739 ...
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bioRxiv - Neuroscience 2023Quote: ... 100,000-125,000 nuclei were used for each CUT&Tag reaction using the CUT&Tag IT Assay Kit (Active Motif, #53610) per manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2023Quote: ... cells were collected after 24 hours of stimulation and performed with cell fractionation using Nuclear Extract Kit (Active Motif 40410). For flow cytometry analysis ...
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bioRxiv - Molecular Biology 2024Quote: Total RNA and subcellular RNA were isolated from cultured cells using the RNA Subcellular Isolation Kit from Active Motif (25501) following the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2021Quote: ... After quenching with 0.5M glycine the HMGN-nucleosome complexes were immunoprecipitated using ChIP-IT Express kit (Active Motif, Cat. No. 53008) with either anti-HMGN1 ...
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bioRxiv - Molecular Biology 2021Quote: ... The purified DNA was analyzed by ChIP-qPCR data relative to input using the mouse negative control and mouse positive control primer set Gapdh in the ChIP-IT qPCR analysis kit (Active Motif). Reaction conditions were programmed with initial denaturation at 50°C for 2 minutes and 95°C for 10 minutes followed by 40 cycles of 95°C for 15 seconds and 60°C for 1 minute ...
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bioRxiv - Cancer Biology 2022Quote: ... Nuclear extraction of MDA-MB-231 cells was done according to manufactureŕs instructions (Universal Magnetic Co-IP Kit, Active Motif, 54002). Imaging flow cytometry analysis was conducted on an Amnis Imagestream Mk (>100,000 events ...
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bioRxiv - Cancer Biology 2020Quote: ... Immunoprecipitated DNA and input DNA were then purified with phenol/chloroform extraction and amplified using GenoMatrix Whole Genome Amplification kit (Active Motif). Quantitative PCR was performed using PowerUP SYBR Green master mix on an ABI StepOnePlus real-time PCR instrument (Applied Biosystems) ...
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bioRxiv - Genomics 2020Quote: ... The DNA samples were processed for library preparation for next-generation sequencing by using a Next Gen DNA Library Kit (Active Motif) following the manufacturer’s manual ...