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Citations for Active Motif :
251 - 300 of 311 citations for Human Bcl2 Associated Death Promoter BAD ELISA Kit since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2021Quote: Chromatin was prepared from flash-frozen liver tissue using the Active Motif ChIP-IT High Sensitivity kit (Active Motif), employing a modified protocol described in (47) ...
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bioRxiv - Cancer Biology 2023Quote: ... Nuclear fractions were extracted following the manufacturer’s instruction supplied with the Universal Magnetic co-IP kit (54002, Active Motif). 1000μg of proteins were immunoprecipitated using 5.0μg of antibody followed by protein G magnetic beads ...
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bioRxiv - Molecular Biology 2024Quote: ATAC-seq was performed as previously published (46) using an ATAC-seq kit (53150, Active motif, Carlsbad, CA, USA) and according to the manufacturer’s protocol on 100,000 cells treated with 30 µM of cPVP or 200 µM of cisplatin for two hours ...
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bioRxiv - Genomics 2024Quote: CUT&Tag was performed as previously described75 using the CUT&Tag-IT Assay Kit (Active Motif, Carlsbad, CA, USA) with modifications recommended in the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2024Quote: ChIP assays were performed using ChIP-IT Express Magnetic Chromatin Immunoprecipitation kit following the manufacturer’s protocol (Active motif, France) and 4 × 106 cells per sample and per epitope ...
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bioRxiv - Cancer Biology 2024Quote: ... histones from A172 cells treated with scramble and ZNF638 siRNA were purified using a histone extraction kit (Active Motif) as per the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2020Quote: ... and nuclear extracts were isolated using the reagents and protocol provided in the Active Motif Nuclear Extract Kit (Active Motif). IL-13 effects on NFκB p52 DNA binding was evaluated using the TransAM NFκB Transcription Factor Assay Kit (Active Motif) ...
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bioRxiv - Immunology 2020Quote: ... Nuclear extract from HuT78 cells (endogenous SP140) was prepared following manufacturer’s protocol (Active Motif nuclear extract and co-IP kit). Protein concentration was determined by Bradford Assay (Pierce) ...
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bioRxiv - Immunology 2024Quote: ... and total RNA fractions were prepared from A549 cells according to the RNA Subcellular Isolation Kit (Active Motif, Cat. #25501). cDNA was prepared as described above and qPCR was performed to analyze VILMIR expression in both cellular fractions ...
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bioRxiv - Neuroscience 2023Quote: ... 100,000-125,000 nuclei were used for each CUT&Tag reaction using the CUT&Tag IT Assay Kit (Active Motif, #53610) per manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2023Quote: ... cells were collected after 24 hours of stimulation and performed with cell fractionation using Nuclear Extract Kit (Active Motif 40410). For flow cytometry analysis ...
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bioRxiv - Molecular Biology 2024Quote: Total RNA and subcellular RNA were isolated from cultured cells using the RNA Subcellular Isolation Kit from Active Motif (25501) following the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2024Quote: Nuclear and whole-cell extracts were prepared from 1.5×107 cells per condition with and without DOX treatment for 4 d using the Nuclear Extract Kit (Active Motif). Co-immunoprecipitation was performed with the nuclear fraction using the Dynabeads Co-Immunoprecipitation Kit (Thermo Fisher ...
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bioRxiv - Molecular Biology 2021Quote: ... After quenching with 0.5M glycine the HMGN-nucleosome complexes were immunoprecipitated using ChIP-IT Express kit (Active Motif, Cat. No. 53008) with either anti-HMGN1 ...
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bioRxiv - Molecular Biology 2021Quote: ... The purified DNA was analyzed by ChIP-qPCR data relative to input using the mouse negative control and mouse positive control primer set Gapdh in the ChIP-IT qPCR analysis kit (Active Motif). Reaction conditions were programmed with initial denaturation at 50°C for 2 minutes and 95°C for 10 minutes followed by 40 cycles of 95°C for 15 seconds and 60°C for 1 minute ...
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bioRxiv - Cancer Biology 2022Quote: ... Nuclear extraction of MDA-MB-231 cells was done according to manufactureŕs instructions (Universal Magnetic Co-IP Kit, Active Motif, 54002). Imaging flow cytometry analysis was conducted on an Amnis Imagestream Mk (>100,000 events ...
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bioRxiv - Cancer Biology 2020Quote: ... Immunoprecipitated DNA and input DNA were then purified with phenol/chloroform extraction and amplified using GenoMatrix Whole Genome Amplification kit (Active Motif). Quantitative PCR was performed using PowerUP SYBR Green master mix on an ABI StepOnePlus real-time PCR instrument (Applied Biosystems) ...
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bioRxiv - Genomics 2020Quote: ... The DNA samples were processed for library preparation for next-generation sequencing by using a Next Gen DNA Library Kit (Active Motif) following the manufacturer’s manual ...
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bioRxiv - Molecular Biology 2021Quote: DNA from whole brain and neuronal nuclei ChIP samples was purified with the Chromatin IP DNA purification kit (Active Motif, #58002) according to the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2022Quote: ... Tagmentation Master Mix was added to the pellet and reaction was incubated in a themomixer at 600-800 rpm set at 37C for 30 min as indicated by manufacturer (ATAC-Seq kit, 53150, Active Motif).
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Alpha-1-antitrypsin binds to the glucocorticoid receptor with biological significance in macrophagesbioRxiv - Immunology 2022Quote: The binding of the p65 subunit of NFκB was quantified using the TransAM™ NFκB p65 kit (Active Motif, Carlsbad, CA), as we previously reported (Bai et al ...
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Epigenetic Changes in Saccharomyces cerevisiae Alters the Aromatic Profile in Alcoholic FermentationbioRxiv - Microbiology 2022Quote: The HDAC inhibition capacity of candidate epigenetic modifiers was measured by a fluorometric HDAC assay kit (Active Motif, Inc., CA, USA), following the manufacturer’s instructions with slight modification to suit the objectives of this research ...
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bioRxiv - Physiology 2022Quote: ... protein from a subset of muscles was fractionated into nuclear and cytoplasmic components using a Nuclear Extract Kit (Active Motif; 40010) according to manufacturer’s instructions ...
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bioRxiv - Neuroscience 2024Quote: Cells from proliferating iMOPs and iMOP-derived neurons were harvested for and processed using the CUT&Tag-IT Assay Kit (Active Motif) described by the manufacturer ...
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bioRxiv - Molecular Biology 2024Quote: ATAC-seq libraries were generated from liver sinusoidal endothelial (LSEC) and liver-resident immune cryopreserved cells using the ATAC-seq kit (Active Motif). H3K27ac histone modification data was generated from liver sinusoidal endothelial (LSEC ...
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bioRxiv - Cancer Biology 2024Quote: ... GSCs (10 million cells / Co-IP) were collected and prepared for nuclear extraction and purification using a nuclear extraction kit (Active Motif). Nuclear extracts were treated with an enzymatic shearing Cocktail for DNA digestion to release undissociated protein complexes ...
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bioRxiv - Cancer Biology 2023Quote: PB NK cells were stimulated or not with IL-12 as previously described and Ch-IP was performed using ChIP-IT PBMCs Kit (Active Motif) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2023Quote: ... genomic DNA was sonicated (300-700 bp size) and MIRA pull-down was performed using the Methyl Collector Ultra Kit (Active Motif), which uses a MBD2b/MBD3L1 protein complex ...
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bioRxiv - Molecular Biology 2023Quote: ... Reactions were carried out according to the manufacturer’s instructions with the Gelshift™ Chemiluminescent EMSA Kit (#37341, Active Motif, Waterloo, Belgium). Purified M35_S protein was diluted in storage buffer to reach the indicated concentration (0.1 to 10 µM ...
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bioRxiv - Molecular Biology 2024Quote: 100K freshly collected IMR90 cells were subjected to ATAC-seq library preparation using the ATAC-seq Kit (Active Motif Cat #53150) according to manufacturer’s protocol ...
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bioRxiv - Genomics 2024Quote: For anti-CENP-A CUT&Tag library preparation was used the CUT&Tag-IT kit from Active Motif (Cat. No. 53160). Each experiment was performed for 500,000 fresh cells ...
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bioRxiv - Cell Biology 2024Quote: ... HDAC1 activity was measured by the addition of a fluorogenic substrate provided by the HDAC1 Assay Kit (Active Motif, Cat#: 56200) and read using a Spectramax iD5 plate reader (Molecular Devices) ...
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bioRxiv - Cell Biology 2024Quote: ... HDAC1 activity was measured by the addition of a fluorogenic substrate provided by the HDAC Assay Kit (Active Motif, Cat#: 56200) and read using a Spectramax iD5 plate reader (Molecular Devices) ...
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bioRxiv - Cancer Biology 2021Quote: The ChIP was performed by using ChIP-IT® Express Kits according to the manufacturer’s instructions (Active Motif, San Diego, CA, USA). For immunoprecipitation ...
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bioRxiv - Neuroscience 2022Quote: ... centrifuged 500 g for 5 min and washed a final time with 1 mL 1X CUT&Tag wash buffer (from CUT&Tag IT Assay Kit, Active Motif, #53610) or 1 mL CUT&RUN 1X wash buffer (1 mL HEPES pH 7.5 ...
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bioRxiv - Molecular Biology 2022Quote: Cleavage under targets and tagmentation (CUT&Tag [35]) was performed for three biological replicates per experimental condition using the CUT&Tag-IT Assay Kit (Anti-Rabbit) (Active Motif, 53160) according to the manufacturer’s instructions with some modifications ...
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bioRxiv - Neuroscience 2021Quote: The rest of the ChIP experiment (beginning from “Protein G Agarose Bead Preparation”) was carried out using the reagents and protocols from the Low Cell ChIP-Seq Kit (Active Motif, 53084). In brief ...
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bioRxiv - Genetics 2022Quote: ... the nuclei suspension was diluted in a 1X volume of 1X Wash Buffer from a CUT&Tag-IT Assay Kit (Active Motif 53160). To estimate the yield of nuclei ...
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bioRxiv - Microbiology 2022Quote: ... parasites were centrifuged 2000xg for 10min and the parasite pellet used directly with the CUT&Tag-IT Assay Kit (Active Motif 53160). Our experimental sample used TgBDP13xHA parasites and 1ul of a 1:50 dilution of the rabbit anti-HA primary antibody (Cell Signaling 3724T) ...
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bioRxiv - Cell Biology 2022Quote: ... Samples in the 300-500bp range were used for immunoprecipitation using the ChIP-IT High Sensitivity kit (Active Motif, cat. no. 53040). DNA was purified using a Zymo DNA Clean & Concentrator-5 kit (Zymo ...
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bioRxiv - Developmental Biology 2023Quote: ... ChIP assay was done using SimpleChIP Plus Enzymatic Chromatin IP kit (magnetic beads) #9005 from CellSignalling and H3K27me3 antibody (Active Motif RRID:AB_2561020). ChIP samples were prepared with Kapa Hyper prep kit ...
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bioRxiv - Cell Biology 2023Quote: ... Reporters and mimics/inhibitors were reverse transfected with Lipofectamine 3000 (Fisher) and luminescence read after 48 hours with the LightSwitch™ Luciferase Assay Kit (Active Motif).
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bioRxiv - Cancer Biology 2021Quote: The effect of CLEFMA on NF-κB p65-DNA interaction was assessed in the nuclear fraction of H441 and CCL151 cells by TransAM NF-κB p65 kit according to the manufacturer’s instructions (Active Motif, Carlsbad, CA, USA). A specific NF-κB inhibitor ...
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bioRxiv - Immunology 2022Quote: ChIP-qPCR analysis were performed using formaldehyde fixed purified BM pDCs with ChIP-IT® Express Chromatin Immunoprecipitation Kits (53035, Active Motif, USA) according to the manufacturer’s instructions ...
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Development of follicular dendritic cells in lymph nodes depends on retinoic acid mediated signalingbioRxiv - Immunology 2020Quote: ... DNA binding capacity of the Nf-κB subunits relB and p52 were determined with the Nf-κB TransAM family kit (Active Motif, Rixensart, Belgium) according to manufacturer’s protocol.
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bioRxiv - Bioengineering 2024Quote: Chromatin immunoprecipitation followed by real-time quantitative PCR (ChIP-qPCR) was performed using the ChIP-IT PBMC kit (53042; Active Motif, Carlsbad CA) according to manufacturer’s instructions ...
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bioRxiv - Microbiology 2022Quote: ... Parasite pellets were then processed as per the manufacturer’s protocol (CUT&Tag-IT™ Assay Kit, Anti-Rabbit, Active motif, Cat no. 53160). Libraries were sequenced by Azenta at PE 2×150 bp.
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bioRxiv - Molecular Biology 2023Quote: Chromatin immunoprecipitation followed by high throughput sequencing (ChIP-seq)59 was performed from HeLa cells using the ChIP-IT High Sensitivity Kit from Active Motif (reference 53040) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2023Quote: ... cells were DNase treated and lysed in ice-cold ATAC seq lysis buffer from the ATAC-Seq Kit (Active Motif, Carlsbad, CA, USA) for 2 min ...
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bioRxiv - Physiology 2024Quote: ... 50,000 nuclei were used for input into a commercially available ATAC-seq kit which includes library preparation (#53150, Active Motif, Inc., CA, USA). We have previously determined that this isolation technique yields ∼70% myosin heavy chain expressing nuclei through RNA-sequencing ...