Labshake search
Citations for Active Motif :
101 - 141 of 141 citations for 7H Cyclopenta c pyridin 7 one 5 6 dihydro 3 methyl 9CI since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Biochemistry 2019Quote: ... at 4°C with Active Motif enzymatic shearing cocktail in 100 µl Complete Digestion Buffer (both from Active Motif), and centrifuged for 10 min ...
-
bioRxiv - Cancer Biology 2020Quote: ... Supershift analysis was carried out by antibodies to RUNX 1 or RUNX 3 (Active Motif cat# 39300 and 39301, respectively). The gel was exposed to X ray film and/or phosphorimaging screens ...
-
bioRxiv - Molecular Biology 2020Quote: ... 5 µl of specific antibody (H3K4me1: Abcam ab8895; H3K27me3: Active Motif 39155; H3K27ac: Active Motif 39133) and beads incubated at 4°C overnight ...
-
bioRxiv - Molecular Biology 2020Quote: ... 5 µl of specific antibody (H3K4me1: Abcam ab8895; H3K27me3: Active Motif 39155; H3K27ac: Active Motif 39133) and beads incubated at 4°C overnight ...
-
bioRxiv - Molecular Biology 2019Quote: ... Chromatin samples were incubated for 5 min at 37°C followed by a second incubation for 10 min at 37°C with shaking at 1,000 rpm after the addition of 1:100 enzymatic working solution (Active Motif, ChIP-IT Enzymatic Kit ...
-
bioRxiv - Genetics 2023Quote: ... chromatin was sonicated for 8 minutes in Buffer 3 (20 s on, 30 s off, 25% amplitude) using an EpiShear probe sonicator (Active Motif).
-
bioRxiv - Microbiology 2022Quote: ... while the rest of the chromatin was immunoprecipitated using 5 μg of Lamin AC antibody (Active Motif), 5 μg of Lamin B1 antibody (Abcam) ...
-
bioRxiv - Neuroscience 2021Quote: ... 5 microliters of the insoluble fraction were then diluted in Complete Lysis Buffer (Active Motif; Carlsbad, CA) and nuclear protein concentration was determined using a Bradford assay (Bio-rad ...
-
bioRxiv - Cancer Biology 2020Quote: ... Nuclear lysates of each tissue specimen were incubated with 5 μg of H3K27ac antibody (Active Motif, 39133) pre-bound to 50 µL magnetic protein A dynabeads (Thermo Fisher Scientific ...
-
bioRxiv - Genomics 2020Quote: ... 5 - 33 μg of sonicated chromatin was incubated with 4 μg ChIP-validated CTCF antibody (Active Motif) overnight on an end-to-end rotator at 4°C ...
-
bioRxiv - Cancer Biology 2023Quote: ... 5 μg of antibodies were used along with 2 μg of Spike-in antibodies (Active Motif, #61686) were bound to 50 μl of Dynabeads Protein G (Thermo Fisher ...
-
bioRxiv - Neuroscience 2020Quote: ... then placed on rotator and incubated overnight at 4°C with 25 uL of Protein G beads and 4 uL (1 mg/mL) anti-RNA polymerase II (102660; Active Motif). Immune complexes were sequentially washed according to kit instructions ...
-
bioRxiv - Molecular Biology 2021Quote: ... Proteins produced in vitro were subjected to interaction assays for 2 hours at 4°C under mild rotation with Drosophila nuclear extracts or recombinant human CTD (Active Motif). Bound proteins were washed 4 times and resuspended in Laemmli buffer for western-blot analysis ...
-
bioRxiv - Cancer Biology 2020Quote: ... The membranes were blocked in 5 % milk/TBST for 1 h followed by primary antibody blotting at 4 °C overnight incubation – 1: 2000 rabbit anti-hmC (cat# 39069, Active Motif). The next day the blots were washed with TBST followed by incubation with secondary 1:2000 anti-rabbit-HRP for 1 h ...
-
bioRxiv - Immunology 2019Quote: ... Blots were incubated over night at 4°C with the following primary antibodies diluted in 2 % skim milk powder/PBS-T: H3K27ac (1:1,000, rabbit, Active Motif, 39133) and H3 (1:10,000 ...
-
bioRxiv - Genomics 2023Quote: ... were pre-coated with antibodies for 4 hours at 4°C and the following antibodies were used: H3K27ac (39685 – Active Motif); H3K9ac (C5B11-9549 – Cell Signalling Technology) ...
-
bioRxiv - Molecular Biology 2019Quote: ... and coupled to one of the following antibodies in the same buffer for 1 hr at room temperature with rotation: 3 μl H3 (Active motif 39763), 5 μl H3K4me3 (Millipore 04-745) ...
-
bioRxiv - Microbiology 2022Quote: ... Membranes were blocked in 5% milk in PBS-T for 1 h at room temperature and incubated overnight at 4°C with primary antibodies against Lamin AC (Active Motif 39287), Lamin B1 (Abcam ab16048) ...
-
bioRxiv - Molecular Biology 2020Quote: ... 5ug antibodies were added to the supernatant and incubated overnight at 4°C with gentle rotation (CTCF, ab70303; RAD21, ab992; H3K4me3, Millipore, 04-745; H3K27ac, Active Motif,39685.). Chromatin was pulled down by protein G Sepharose beads (#17061801 ...
-
bioRxiv - Genetics 2020Quote: ... Each ChIP used 2μg sheared chromatin and was incubated overnight (16 hours) at 4°C with either 5μg of anti-H3K9ac (39137, Active Motif, Carlsbad, CA), or 5μg of anti-H3K27ac (39133 ...
-
bioRxiv - Genomics 2021Quote: ... ChIP enrichment was performed by incubation for 16 h at 4°C with the following antibodies: 2 µg H3K27ac antibody (Active Motif #39133), 4 µg H3K4me1 antibody (Millipore 07-436) ...
-
bioRxiv - Genetics 2019Quote: ... Binding reactions for super shift assays were performed for 30 min at 4-8°C using the Binding Buffer B-1 (Active Motif 37480) together with Stabilizing Solution D (Active Motif 37488 ...
-
bioRxiv - Developmental Biology 2020Quote: ... Beads were then resuspended in Blocking buffer and coated with antibodies for 4 h at 4°C (H4K20me1 [0.5 μg/IP], Active Motif cat. 39727). Once coated beads were magnet-separated and resuspended in 1 mL of lysate ...
-
bioRxiv - Molecular Biology 2019Quote: We performed immunoprecipitations with 5 μg 1°Ab (Target: SATB2, Abcam, positive control: RNA pol II, Active Motif, negative control ...
-
bioRxiv - Genetics 2022Quote: ... Around 20 μg or 10 μg of chromatin were incubated overnight at 4°C with 10 μg H3.X/Y or 2.5 μg H3K9me3 antibody respectively (Active Motif-61161, Abcam-ab8898). Chromatinantibody extracted with incubated with protein G Dynal beads (Thermo Fisher Scientific ...
-
bioRxiv - Molecular Biology 2023Quote: ... All incubations with primary antibodies were performed for either 1h at room temperature or overnight at 4°C with the following antibodies for H3K9me3 (Active Motif, 1:1000), DAXX (Santa Cruz ...
-
Transcription factor LSF facilitiates lysine methylation of α-tubulin by microtubule-associated SET8bioRxiv - Biochemistry 2019Quote: ... 1 mg of total HEK293T cellular extract was incubated with 5 μg of anti-SET8 antibody (Active Motif, 61009), anti-LSF antibody (Millipore,17-10252) ...
-
bioRxiv - Developmental Biology 2019Quote: ... 5 µl was transformed into chemically competent TAM-1 cells in a 96-well plate format (Active Motif #11096) and selected for Chloramphenicol resistance ...
-
bioRxiv - Microbiology 2023Quote: ... The membranes were blocked in 5% milk and incubated with anti-CTCF antibody (Active Motif 61311; 1:1,000 dilution), anti-β-actin antibody (Sigma-Aldrich ...
-
bioRxiv - Cell Biology 2021Quote: ... Equal intensity of different RNA probes were mixed with equal molar of different C/EBPα proteins individually in EMSA buffer B1 (Active Motif, Cat. No. 37480), stabilizing solution D (Active Motif ...
-
bioRxiv - Neuroscience 2021Quote: ... The following primary antibodies were diluted in blocking buffer and applied to cells overnight at 4°C to label key histone modifications associated with epigenetic regulation: rabbit H3K9me3 (1:500; Active Motif, 39765; Carlsbad, CA, USA); mouse H3K4me1 (1:100 ...
-
bioRxiv - Developmental Biology 2020Quote: ... pre-cleared for 2 h followed by immunoprecipitation with primary antibodies for 12-16 hours at 4°C (H3K27ac, Active motif 39133; H3K27me3, CST 9733s). 5% of samples were used as input DNA ...
-
bioRxiv - Molecular Biology 2022Quote: ... After an overnight incubation at 4°C with the primary antibodies (anti-5mC, Eurogentec, ref BI-MECY-0100, 1/500; anti-5hmC, Active Motif, ref 39569, 1/500), and a 1-hour incubation at room temperature with secondary antibodies (Dylight 488 anti-mouse ...
-
bioRxiv - Plant Biology 2021Quote: ... 30 ml cell suspension were sonicated for 5 min (5s on/10s off, 60% amplitude) with a sonicator (EpiShear, Active Motif) equipped with a ¼” microtip probe ...
-
bioRxiv - Genetics 2021Quote: ... quenched by 0.125M glycine for 5 min at RT and chromatin was then sheared by 5 min sonication (25% amplitude, 30sec off and 30 sec on) using a probe sonicator (Epishear, Active Motif). Immunoprecipitation was performed with Protein G Dynabeads (Life Technologies) ...
-
bioRxiv - Synthetic Biology 2021Quote: ... half of each original cell aliquot was incubated with Dynabeads protein-G conjugated with 5 µg of rabbit polyclonal to Histone H3K27me3 (Active motif 39155) or rabbit polyclonal to Histone H3 (acetyl K27 ...
-
bioRxiv - Microbiology 2023Quote: ... Beads were resuspended in 100 μl of IP/wash buffer and coated with 5 μg of rabbit anti-m6A antibody (Active motif, 61495) for 45 min at room temperature with rotation ...
-
bioRxiv - Genomics 2019Quote: ... 5 million cells per replicate were incubated with 5 ug (Histone) or 7.5 ug (Oct4) antibody overnight at 4’C (antibodies: H3K9me3: abcam AB8898; H3K27Ac: Active Motif 39133; H3K4me3: Active Motif 39159; Oct4: Santa Cruz sc-8629). Antibody-bound chromatin was incubated with Protein G Dynabeads (Invitrogen ...
-
bioRxiv - Molecular Biology 2023Quote: ... 100 mM NaCl. 5 nM of full length purified ORC1 (Cusabio Catalog N. CSB-BP621667HU(A4) and H2A.Z (Active Motif, Cat. No. 81072) proteins were incubated with 1.1 nM of 32P-labeled single-stranded (ss ...
-
bioRxiv - Developmental Biology 2023Quote: ... Human induced pluripotent stem cell derived cardiomyocytes (iPSC-CMs) from four HLHS patients were fixed in 4% paraformaldehyde and stained with antibody against 5-methylcytosine (Active Motif, RRID: AB_2687950)31 ...
-
bioRxiv - Microbiology 2024Quote: ... The primary antibodies were used at the following dilutions: anti-Cas9 (1:1000 in 5% milk/PBS-T, Active Motif, clone 7A9-3A3); anti-EF1α (1:20,000 in 1% milk/PBS-T ...