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Citations for Active Motif :
51 - 100 of 128 citations for SARS CoV 2 Spike Glycoprotein S2 Sheep Fc Tag HEK293 Biotin since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genomics 2022Quote: ... Soluble material was combined with 50 ng spike-in chromatin (Active Motif 53083) and 5% was reserved as input DNA ...
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bioRxiv - Neuroscience 2022Quote: ... centrifuged 500 g for 5 min and washed a final time with 1 mL 1X CUT&Tag wash buffer (from CUT&Tag IT Assay Kit, Active Motif, #53610) or 1 mL CUT&RUN 1X wash buffer (1 mL HEPES pH 7.5 ...
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bioRxiv - Neuroscience 2022Quote: ... and the CUT&Tag-IT Assay Kit (Active Motif) following the manufacture’s manual ...
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bioRxiv - Cell Biology 2023Quote: ... 100 µg of chromatin supplemented with 10 ng of Spike-in chromatin (Active Motif) were used per ChIP experiment ...
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bioRxiv - Genetics 2024Quote: CUT&Tag analysis of NaBu treated CCD841 cells and mouse intestinal samples were performed using CUT&Tag-IT™ assay kits (Active Motif # 53160, 53170). Approximately 30 mg of tissue was homogenized per reaction by first chopping frozen tissue into smaller pieces in a petri dish with a razor blade on dry ice ...
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bioRxiv - Genomics 2022Quote: ... at a concentration of 10 ng/million cells and spike-in antibody (Active Motif, 61686) at a concentration of 0.4 µg/million cells were added following lysis and sonication but prior to immunoprecipitation ...
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bioRxiv - Cancer Biology 2022Quote: ... we employed Active Motif ChIP-seq spike-in using Drosophila chromatin (Active Motif catalogue # 53083) and an antibody against Drosophila specific histone variant H2Av (Active Motif ...
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bioRxiv - Developmental Biology 2022Quote: ... 0.5-1 μg of primary antibody with 0.25 μg Spike-in antibody (Active Motif 61686) diluted in Digitonin block buffer was added to the bead-nuclei mixture and incubated for 3 hours (histone marks ...
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bioRxiv - Neuroscience 2023Quote: ... nuclei pellet was washed once with 1 mL Nuclei Wash Buffer and then once with 1 mL of 1X CUT&Tag Wash Buffer (from Active Motif CUT&Tag IT Assay kit). Remove CUT&Tag Wash Buffer ...
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bioRxiv - Genomics 2023Quote: ... Each IP was spiked with 90ng of Drosophila spike-in chromatin (Active Motif cat. no. 53083) and 0.5 μg of Drosophila-specific H2Av antibody was added to each sample.
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bioRxiv - Genomics 2023Quote: ... Each IP was spiked with 10ng of Drosophila spike-in chromatin (Active Motif cat. no. 53083) and 0.5 μg of Drosophila-specific H2Av antibody was added to each sample and incubated overnight with rotation at 4°C ...
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bioRxiv - Molecular Biology 2019Quote: ... 25 µg of sample chromatin was mixed with 50 ng spike- in Drosophila chromatin (53083; Active Motif). Mixture of experimental chromatin and spike- in chromatin was then incubated with a mix containing 4 µg of H3K4me3 antibody (CS200580 ...
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bioRxiv - Molecular Biology 2020Quote: ... 4 - 10 µg of target antibodies and 2μg of spike-in antibody (anti-H2Av, Active Motif, #61686) were added and incubated on a rotator at 4°C overnight ...
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bioRxiv - Molecular Biology 2022Quote: ... chromatin from 1 x 107 cells were mixed with 20 ng spike-in chromatin (Active Motif, #53083) and incubated with 4-8 μg primary target antibody and 2 μg spike-in antibody (Active Motif ...
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bioRxiv - Pathology 2023Quote: ... ChIP reactions were supplemented with 10 ng of Drosophila melanogaster chromatin (spike in chromatin, Active motif, 08221011), and 1 μg of an antibody recognizing H2Av ...
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bioRxiv - Cancer Biology 2023Quote: ... 30 μg of shared chromatin and 20 ng of spike-in chromatin from Drosophila (Active Motif, #53083) were incubated with antibodies/beads mixture overnight at 4°C ...
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bioRxiv - Genomics 2023Quote: ... 0.5 million cells were processed using the CUT&Tag-IT kit (Active Motif) as per manufacturer’s instructions and the resulting libraries were paired-end sequenced on a NextSeq500 platform (Illumina ...
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bioRxiv - Molecular Biology 2019Quote: ... using 10mg of antibody abcam ab992 together with 40ng of Drosophila melanogaster spike-in chromatin (Active motif 53083) and spike-in antibody (Active motif 61686) ...
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bioRxiv - Biochemistry 2020Quote: ... the spike-in chromatin and antibody were added in the reaction as per the manufacturer’s recommendation (Active Motif). The chromatin was incubated with antibodies at 4 °C overnight and then added to 30 μl of protein G-Dyna beads (Thermo Fisher Scientific ...
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bioRxiv - Cancer Biology 2020Quote: ... and 10 mM glycerophosphate) with the addition of 40 ng Spike-in Chromatin per reaction (Active Motif 53083). Immunoprecipitation was conducted using 4 µg of anti-H3 (Abcam ab1791) ...
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bioRxiv - Biochemistry 2020Quote: ... the spike-in chromatin and antibody were added in the reaction as per the manufacturer’s recommendation (Active Motif). The chromatin was incubated with antibodies at 4 °C overnight and then added to 30 μl of protein G-Dyna beads (Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2020Quote: ... Recombinant Human DNMT1 with an N-terminal GST tag was purchased from Active Motif and Pierce Glutathione Magnetic Beads (Thermo Scientific ...
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bioRxiv - Genomics 2022Quote: ... we used CUT&Tag-IT™ assay kit (Cat. No. 53160) from Active Motif® ...
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bioRxiv - Cancer Biology 2020Quote: ... the complex was biotin-labeled via incubation with recombinant sortase A5 enzyme (Active Motif) and a custom tri-glycine biotin peptide (Thermo Fisher) ...
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bioRxiv - Biophysics 2019Quote: ... and the mix was incubated overnight at 4 ºC with antibodies against MeCP2 (D4F3, Cell Signalling) plus spike-in antibody (Active Motif). For ChIP-seq library preparation ...
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bioRxiv - Developmental Biology 2020Quote: ... ChIP-Seq of T7 and MED12 were done in the presence of Drosophila spike-in chromatin and antibody following the manufacturers’ protocol (Active Motif). ChIP-Seq library construction was done using NEBNext Ultra™ II DNA Library Prep Kit for Illumina (NEB ...
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bioRxiv - Neuroscience 2022Quote: CUT&Tag was performed from 450k nuclei using the Cut&TagIT Kit following manufacturer’s protocol (Active Motif).
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bioRxiv - Genomics 2022Quote: ... End repair was carried out by adding a 30 μl solution containing 0.5 mM biotin-14-dATP (Active Motif 14138), 0.5 mM biotin-14-dCTP (AAT bio 17019) ...
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bioRxiv - Neuroscience 2024Quote: Cells from proliferating iMOPs and iMOP-derived neurons were harvested for and processed using the CUT&Tag-IT Assay Kit (Active Motif) described by the manufacturer ...
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bioRxiv - Molecular Biology 2022Quote: Cleavage under targets and tagmentation (CUT&Tag [35]) was performed for three biological replicates per experimental condition using the CUT&Tag-IT Assay Kit (Anti-Rabbit) (Active Motif, 53160) according to the manufacturer’s instructions with some modifications ...
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bioRxiv - Genetics 2022Quote: ... the nuclei suspension was diluted in a 1X volume of 1X Wash Buffer from a CUT&Tag-IT Assay Kit (Active Motif 53160). To estimate the yield of nuclei ...
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bioRxiv - Microbiology 2022Quote: ... parasites were centrifuged 2000xg for 10min and the parasite pellet used directly with the CUT&Tag-IT Assay Kit (Active Motif 53160). Our experimental sample used TgBDP13xHA parasites and 1ul of a 1:50 dilution of the rabbit anti-HA primary antibody (Cell Signaling 3724T) ...
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bioRxiv - Microbiology 2022Quote: ... Parasite pellets were then processed as per the manufacturer’s protocol (CUT&Tag-IT™ Assay Kit, Anti-Rabbit, Active motif, Cat no. 53160). Libraries were sequenced by Azenta at PE 2×150 bp.
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bioRxiv - Molecular Biology 2021Quote: ... Immunoprecipitations for H2A.Z and HA-tag were performed similarly on benzonase-treated yeast cell extract using 36µl Protein-A sepharose beads coupled with 6µl of appropriate antibody (Anti-H2A.Z, Active Motif 39647; Anti-HA tag, Abcam ab9110). Control immunoprecipitations (mock ...
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bioRxiv - Molecular Biology 2023Quote: CUT&Tag was performed as described previously (Kaya-Okur et al., 2020; Kaya-Okur et al., 2019) using the CUT&Tag-IT Assay Kit from Active Motif (Catalog No. 53160) and following manufacturer’s instructions ...
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bioRxiv - Neuroscience 2019Quote: ... 2 μM Daminozide (Active Motif), 10 μM Paragyline Hydrochloride (Sigma)) ...
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bioRxiv - Molecular Biology 2024Quote: ... H3K27ac histone modification data was generated from liver sinusoidal endothelial (LSEC) and liver-resident immune cryopreserved cells using the Cut&Tag-IT Assay kit (Active Motif, H3K27ac antibody cat#39133). Library products were assessed by Agilent Bioanalyzer HS DNA assays (Agilent Technologies ...
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bioRxiv - Developmental Biology 2019Quote: ... MEIS1/2/3 (Active Motif 39796) and LTL (Vectorshield FL-1321) ...
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bioRxiv - Biochemistry 2020Quote: ... 2 µL PRC2 (2.8 µM, Active Motif), 42.47 µL water ...
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bioRxiv - Genomics 2021Quote: ... 2 µg H3K4me3 antibody (Active Motif #39915), or 2 µg RNA Polymerase II (PolII ...
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bioRxiv - Molecular Biology 2023Quote: ... and 2 µg H3K9me3 antibody (39161, Active Motif). Samples were incubated for 3h at 4°C ...
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bioRxiv - Cancer Biology 2023Quote: ... Pol II ser 2 phos (Active Motif C1984), yH2AX (abcam ab11174) ...
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bioRxiv - Genomics 2023Quote: ... 2 μg of anti-His2Av (Active Motif 61686) were added to each immunoprecipitation for spike-in purposes ...
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bioRxiv - Cancer Biology 2023Quote: 2 × 107 cells for H3K27ac (Active Motif, Cat # 39133) and 3 × 107 cells for IRF4 (Santa Cruz Biotechnology ...
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bioRxiv - Cancer Biology 2023Quote: ... and Negative Primer Set 1 and 2 (Active Motif) as positive and negative controls for p-STAT4 binding (see table S1 for sequences) ...
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bioRxiv - Molecular Biology 2022Quote: ... along with 2 µg of anti-H2Av (Active Motif, #61686) [which recognizes Drosophila-specific histone variant H2Av in spike-in chromatin] ...
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bioRxiv - Genomics 2021Quote: ... 2 μg for anti-CTCF (Active Motif (Carlsbad, CA, USA) 61312) ...
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bioRxiv - Genomics 2021Quote: ... 0.5% Triton X-100) and 2 μL 5mC antibody (Active Motif 61479) were added ...
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bioRxiv - Genomics 2023Quote: ... then incubated with 2 µg/mL dilution 5hmC antibody (Active Motif, 39092) overnight at 4°C in a humidified chamber ...
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bioRxiv - Developmental Biology 2023Quote: ... mouse anti-MEIS1/2/3 (0.5 µg/ml, 39795, Active Motif, RRID:AB_2750570), rabbit anti-DHRS3 (1:500 ...